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Full-Text Articles in Life Sciences
Wrinkled1, A Ubiquitous Regulator In Oil Accumulating Tissues From Arabidopsis Embryos To Oil Palm Mesocarp, Wei Ma, Que Kong, Vincent Arondel, Aruna Kilaru, Philip D. Bates, Nicholas A. Thrower, Christoph Benning, John B. Ohlrogge
Wrinkled1, A Ubiquitous Regulator In Oil Accumulating Tissues From Arabidopsis Embryos To Oil Palm Mesocarp, Wei Ma, Que Kong, Vincent Arondel, Aruna Kilaru, Philip D. Bates, Nicholas A. Thrower, Christoph Benning, John B. Ohlrogge
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WRINKLED1 (AtWRI1) is a key transcription factor in the regulation of plant oil synthesis in seed and non-seed tissues. The structural features of WRI1 important for its function are not well understood. Comparison of WRI1 orthologs across many diverse plant species revealed a conserved 9 bp exon encoding the amino acids “VYL”. Site-directed mutagenesis of amino acids within the ‘VYL’ exon of AtWRI1 failed to restore the full oil content of wri1-1 seeds, providing direct evidence for an essential role of this small exon in AtWRI1 function. Arabidopsis WRI1 is predicted to have three alternative splice …