Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
Differential Activation Of The Tcpph Promoter By Aphb Determines Biotype Specificity Of Virulence Gene Expression In Vibrio Cholerae, Gabriela Kovacikova, Karen Skorupski
Differential Activation Of The Tcpph Promoter By Aphb Determines Biotype Specificity Of Virulence Gene Expression In Vibrio Cholerae, Gabriela Kovacikova, Karen Skorupski
Dartmouth Scholarship
Vibrio cholerae strains of the classical biotype express the genes encoding cholera toxin (CT) and toxin- coregulated pilus (TCP) under a variety of environmental conditions in vitro, whereas El Tor biotype strains express these genes only under specialized culture conditions. We show here that a single base-pair difference at positions 2 65 and 2 66 of the classical and El Tor tcpPH promoters, respectively, is responsible for the differential regulation of virulence gene expression in these two disease-causing biotypes. Analysis of tcpP-lacZ fusions in both V. cholerae and Escherichia coli indicated that transcriptional activation of the El Tor tcpPH promoter …