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Dartmouth College

Series

1998

Arabidopsis

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The Carg Boxes In The Promoter Of The Arabidopsis Floral Organ Identity Gene Apetala3 Mediate Diverse Regulatory Effects, Joline J. Tilly, David W. Allen, Thomas Jack Apr 1998

The Carg Boxes In The Promoter Of The Arabidopsis Floral Organ Identity Gene Apetala3 Mediate Diverse Regulatory Effects, Joline J. Tilly, David W. Allen, Thomas Jack

Dartmouth Scholarship

APETALA3 is a MADS box gene required for normal development of the petals and stamens in the Arabidopsis flower. Studies in yeast, mammals and plants demonstrate that MADS domain transcription factors bind with high affinity to a consensus sequence called the CArG box. The APETALA3 promoter contains three close matches to the consensus CArG box sequence. To gain insights into the APETALA3 regulatory circuitry, we have analyzed the APETALA3 promoter using AP3::uidA(GUS) fusions. 496 base pairs of APETALA3 promoter sequence 5′ to the transcriptional start directs GUS activity in the same temporal and spatial expression pattern as the APETALA3 RNA …