Open Access. Powered by Scholars. Published by Universities.®
- Discipline
- Keyword
-
- Amino acid sequence (2)
- Base sequence (2)
- Fungal (2)
- Genetics (2)
- Metabolism (2)
-
- Molecular sequence data (2)
- Saccharomyces cerevisiae (2)
- American redstart (1)
- Binding sites (1)
- Biological transport (1)
- Biosynthesis (1)
- Cell nucleus (1)
- Chromosome mapping (1)
- Dna (1)
- Dna primers (1)
- Dna-binding proteins (1)
- Fluorescence (1)
- Foraging behavior (1)
- Fungal proteins (1)
- Gene deletion (1)
- Gene expression regulation (1)
- Genes (1)
- Growth & development (1)
- In situ hybridization (1)
- Jamaica (1)
- Membrane proteins (1)
- Messenger (1)
- Mutation (1)
- Neotropical migrant (1)
- Nuclear envelope (1)
Articles 1 - 11 of 11
Full-Text Articles in Life Sciences
Mutation Or Deletion Of The Saccharomyces Cerevisiae Rat3/Nup133 Gene Causes Temperature-Dependent Nuclear Accumulation Of Poly(A)+ Rna And Constitutive Clustering Of Nuclear Pore Complexes, Ou Li, Catherine V. Heath, David C. Amberg, Thomas C. Dockendorff, Connie S. Copeland, Michael Snyder, Charles N. Cole
Mutation Or Deletion Of The Saccharomyces Cerevisiae Rat3/Nup133 Gene Causes Temperature-Dependent Nuclear Accumulation Of Poly(A)+ Rna And Constitutive Clustering Of Nuclear Pore Complexes, Ou Li, Catherine V. Heath, David C. Amberg, Thomas C. Dockendorff, Connie S. Copeland, Michael Snyder, Charles N. Cole
Dartmouth Scholarship
To identify genes whose products play potential roles in the nucleocytoplasmic export of messenger RNA, we isolated temperature-sensitive strains of Saccharomyces cerevisiae and examined them by fluorescent in situ hybridization. With the use of a digoxigen-tagged oligo-(dT)50 probe, we identified those that showed nuclear accumulation of poly(A)+ RNA when cells were shifted to the nonpermissive temperature. We describe here the properties of yeast strains bearing the rat3-1 mutation (RAT-ribonucleic acid trafficking) and the cloning of the RAT3 gene. When cultured at the permissive temperature of 23 degrees C, fewer than 10% of cells carrying the rat3-1 allele showed nuclear accumulation …
Foraging Behavior Of American Redstarts In Breeding And Wintering Habitats: Implications For Relative Food Availability, Irby J. Lovette, Richard T. Holmes
Foraging Behavior Of American Redstarts In Breeding And Wintering Habitats: Implications For Relative Food Availability, Irby J. Lovette, Richard T. Holmes
Dartmouth Scholarship
We investigated food availability for a long-distance migrant species, the American Redstart (Setophaga ruticilla), in both its summer breeding habitat in New Hampshire and in its winter habitat in Jamaica. We used four components of foraging behavior (prey attack rate, foraging speed, time spent foraging, and foraging maneuver use) as indicators of the relative availability of prey in the two seasons. Redstarts attacked prey at a significantly greater rate in summer than in winter, indicating that foraging birds encountered prey more frequently in summer. The winter prey-encounter rate was low even though redstarts moved almost twice as fast …
Transactivation Of The Moloney Murine Leukemia Virus And T-Cell Receptor Beta-Chain Enhancers By Cbf And Ets Requires Intact Binding Sites For Both Proteins., Wanwen Sun, Barbara J. Graves, Nancy A. Speck
Transactivation Of The Moloney Murine Leukemia Virus And T-Cell Receptor Beta-Chain Enhancers By Cbf And Ets Requires Intact Binding Sites For Both Proteins., Wanwen Sun, Barbara J. Graves, Nancy A. Speck
Dartmouth Scholarship
The Moloney murine leukemia virus (Mo-MLV) enhancer contains binding sites (LVb and LVc) for the ets gene family of proteins and a core site that binds the polyomavirus enhancer-binding protein 2/core-binding factor (cbf) family of proteins. The LVb and core sites in the Mo-MLV enhancer contribute to its constitutive activity in T cells. All three binding sites (LVb, LVc, and core) are required for phorbol ester inducibility of the Mo-MLV enhancer. Adjacent binding sites for the ets and cbf proteins likewise constitute a phorbol ester response element within the human T-cell receptor beta-chain (TCR beta) enhancer and contribute to constitutive …
Oxygen Control Of The Bradyrhizobium Japonicum Hema Gene., Karen M. Page, Mary Lou Guerinot
Oxygen Control Of The Bradyrhizobium Japonicum Hema Gene., Karen M. Page, Mary Lou Guerinot
Dartmouth Scholarship
The hemA gene of Bradyrhizobium japonicum, which encodes the first enzyme in the heme biosynthetic pathway, is regulated by oxygen. Up to ninefold induction of beta-galactosidase activity is seen when cultures of B. japonicum containing either a plasmid-encoded or a chromosomally integrated hemA-lacZ fusion are shifted to restricted aeration. The oxygen effect is mediated via the FixLJ two-component regulatory system, which regulates the expression of a number of genes involved in the nitrogen fixation process in response to low-oxygen conductions; oxygen induction is lost when the hemA-lacZ fusion is expressed in strains of B. japonicum carrying mutations in fixL or …
A Tef-1-Independent Mechanism For Activation Of The Simian Virus 40 (Sv40) Late Promoter By Mutant Sv40 Large T Antigens., Paul Casaz, Phillip W. Rice, Charles N. Cole, Ulla Hansen
A Tef-1-Independent Mechanism For Activation Of The Simian Virus 40 (Sv40) Late Promoter By Mutant Sv40 Large T Antigens., Paul Casaz, Phillip W. Rice, Charles N. Cole, Ulla Hansen
Dartmouth Scholarship
Simian virus 40 (SV40) large tumor antigen (T antigen) stimulates the activity of the SV40 late promoter and a number of cellular and other viral promoters. We have characterized the ability of T antigens with mutations in the DNA-binding domain and within the N-terminal 85 residues to activate the SV40 late promoter. T antigens lacking both nonspecific and sequence-specific DNA-binding activities were able to induce the late promoter. Mutations within the N-terminal 85 residues of T antigen diminished activation by less than twofold. Activation by wild-type and most of the mutant T antigens required intact binding sites for the cellular …
3 Genes Of The Map Kinase Cascade, Mek-2, Mpk-1/Sur-1 And Let-60 Ras, Are Required For Meiotic Cell-Cycle Progression In Caenorhabditis-Elegans, Diane L. Church, Kun Liang Guan, Eric J. Lambie
3 Genes Of The Map Kinase Cascade, Mek-2, Mpk-1/Sur-1 And Let-60 Ras, Are Required For Meiotic Cell-Cycle Progression In Caenorhabditis-Elegans, Diane L. Church, Kun Liang Guan, Eric J. Lambie
Dartmouth Scholarship
In the germline of Caenorhabditis elegans hermaphrodites, meiotic cell cycle progression occurs in spatially restricted regions. Immediately after leaving the distal mitotic region, germ cells enter meiosis and thereafter remain in the pachytene stage of first meiotic prophase for an extended period. At the dorsoventral gonadal flexure, germ cells exit pachytene and subsequently become arrested in diakinesis. We have found that exit from pachytene is dependent on the function of three members of the MAP kinase signaling cascade. One of these genes, mek-2, is a newly identified C. elegans MEK (MAP kinase kinase). The other two genes, mpk-1/sur-1 (MAP kinase) …
Transcriptional Activity Of Core Binding Factor-Alpha (Aml1) And Beta Subunits On Murine Leukemia Virus Enhancer Cores., Ari L. Zaiman, Amy F. Lewis, Barbara E. Crute, N. A. Speck, Jack Lenz
Transcriptional Activity Of Core Binding Factor-Alpha (Aml1) And Beta Subunits On Murine Leukemia Virus Enhancer Cores., Ari L. Zaiman, Amy F. Lewis, Barbara E. Crute, N. A. Speck, Jack Lenz
Dartmouth Scholarship
Core binding factor (CBF), also known as polyomavirus enhancer-binding protein 2 and SL3 enhancer factor 1, is a mammalian transcription factor that binds to an element termed the core within the enhancers of the murine leukemia virus family of retroviruses. The core elements of the SL3 virus are important genetic determinants of the ability of this virus to induce T-cell lymphomas and the transcriptional activity of the viral long terminal repeat in T lymphocytes. CBF consists of two subunits, a DNA binding subunit, CBF alpha, and a second subunit, CBF beta, that stimulates the DNA binding activity of CBF alpha. …
Protein-Peptide Interactions Analyzed With The Yeast Two-Hybrid System, Meijia Yang, Zining Wu, Stanley Fields
Protein-Peptide Interactions Analyzed With The Yeast Two-Hybrid System, Meijia Yang, Zining Wu, Stanley Fields
Dartmouth Scholarship
The yeast two-hybrid system was used to screen a library of random peptides fused to a transcriptional activation domain in order to identify peptides capable of binding to the retinoblastoma protein (Rb). Seven peptides were identified, allof which contain the Leu-X-Cys-X-Glu motif found in Rb-binding proteins, although their activity in the yeast assay variedover a 40-fold range. Mutagenesis of the DNA encoding two of these peptides followed by screening in the two-hybrid systemallowed the delineation of residues apart from the invariant Leu, Cys and Glu that affect binding to Rb. Binding affinities of a peptide and one of its variants …
Strain-Dependent Variation In Carbon Source Regulation Of Nucleus-Encoded Mitochondrial Proteins Of Saccharomyces Cerevisiae., Timothy A. Brown, Bernard L. Trumpower
Strain-Dependent Variation In Carbon Source Regulation Of Nucleus-Encoded Mitochondrial Proteins Of Saccharomyces Cerevisiae., Timothy A. Brown, Bernard L. Trumpower
Dartmouth Scholarship
Nuclear genes encoding mitochondrial proteins are regulated by carbon source with significant heterogeneity among four Saccharomyces cerevisiae strains. This strain-dependent variation is seen both in respiratory capacity of the cells and in the expression of beta-galactosidase reporter fusions to the promoters of CYB2, CYC1, CYC3, MnSOD, and RPO41.
Richard Goldschmidt's "Heresies" And The Evolutionary Synthesis, Michael Dietrich
Richard Goldschmidt's "Heresies" And The Evolutionary Synthesis, Michael Dietrich
Dartmouth Scholarship
No abstract provided.
A Novel Translational Regulation Function For The Simian Virus 40 Large-T Antigen Gene., Prithi Rajan, Sathyamagalam Swaminathan, Jiyue Zhu, Charles N. Cole
A Novel Translational Regulation Function For The Simian Virus 40 Large-T Antigen Gene., Prithi Rajan, Sathyamagalam Swaminathan, Jiyue Zhu, Charles N. Cole
Dartmouth Scholarship
Cells use the interferon-induced, double-stranded-RNA-dependent protein kinase PKR as a defense against virus infections. Upon activation, PKR phosphorylates and thereby inactivates the protein synthesis initiation factor eIF-2, resulting in the cessation of protein synthesis. Viruses have evolved various strategies to counteract this cellular defense. In this paper, we show that simian virus 40 (SV40) large-T antigen can antagonize the translational inhibitory effect resulting from the activation of PKR in virus-infected cells. Unlike the situation with other virus-host cell interactions, SV40 large-T antigen does not block the activation of PKR, suggesting that SV40 counteracts the cellular antiviral response mediated by PKR …