Life Sciences Commons^™

Effect Of Hydrogen Peroxide On The Biosynthesis Of Heme And Proteins: Potential Implications For The Partitioning Of Glu-TrnaGlu Between These Pathways, Carolina Farah, Gloria Levicán, Michael Ibba, Omar Orellana

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Glutamyl-tRNA (Glu-tRNA^Glu) is the common substrate for both protein translation and heme biosynthesis via the C5 pathway. Under normal conditions, an adequate supply of this aminoacyl-tRNA is available to both pathways. However, under certain circumstances, Glu-tRNA^Glu can become scarce, resulting in competition between the two pathways for this aminoacyl-tRNA. In Acidithiobacillus ferrooxidans, glutamyl-tRNA synthetase 1 (GluRS1) is the main enzyme that synthesizes Glu-tRNA^Glu. Previous studies have shown that GluRS1 is inactivated in vitro by hydrogen peroxide (H₂O₂). This raises the question as to whether H₂O₂ negatively affects …

What’S Cookin’ Good Lookin’: The Rise And Phenomena Of The Female Foodie Performer Through Social Media, Alison Weiss

Student Scholar Symposium Abstracts and Posters

Once seen in black and white terms as human sustenance or luxury, eating has become not only a hobby, but an obsession. Whereas chefs and cooks were previously regarded as average, behind-the-scenes workers, they have now stepped out from the kitchen and into the spotlight, becoming celebrated public idols – and performers. With images tailored to different demographics right down to their clothing and hairstyles, chefs and cooks no longer merely prepare food: they put on a show. The foodie phenomena has been pioneered by females, largely in part to the parallel-running infatuation with health, fitness, and food trends that …

Dancing Through Life: Allosteric Transitions And Structural Analysis Of Hsp70 And Hsp110 Chaperone Proteins, Gabrielle Stetz, Gennady M. Verkhivker

Student Scholar Symposium Abstracts and Posters

The molecular chaperone protein Hsp70 is centrally involved in cellular homeostasis by assisting in the folding and degradation of protein substrates. Hsp70 is joined by co-chaperones, such as Hsp110, which contribute to specialized tasks of the Hsp70 complex. Imbalances of this heat shock protein system are believed to be involved with the deregulation of cancer pathways and other human diseases. Better understanding of how these heat shock proteins work at the molecular level, which has been investigated using molecular docking tools, will give more clues about biological function. Simulating the formation and function of Hsp70 based chaperone complexes could provide …

The Non-Lethal Effects Of Climate Change On The Territoriality Of Lottia Gigantea, Tracey Gunanto, Christina Chavez, Jessica Martinez, William G. Wright

Student Scholar Symposium Abstracts and Posters

The intertidal zone has been described as ground zero for global warming. Here, the owl limpet, Lottia gigantea, adapted to the cool ocean temperatures, must withstand a few hours of baking sun during day-time low tides. This hardship is predicted to increase in frequency and severity in the future as the globe warms. Our research hypothesized that heat events compromise territorial behavior of L. gigantea. All observations and experiments were performed at Inspiration Point near Newport Beach, California. We measured the natural radiant temperature of tagged limpets during day-time low tides using a field-calibrated infrared “thermogun”. We also …

Theatre For Development: “The Wanna Be”, Joshua Dominguez

Student Scholar Symposium Abstracts and Posters

The college experience in American culture is a popular topic that is being questioned throughout the media. It is being questioned on a weekly basis in today’s media and brings to light issues that have not been questioned for decades. Some of the main issues such as diversity within institutions, the "Greek System", and sexual assault are all being spotlighted and widely advertised as problems that need focusing on putting an end to. This new era of college students are being challenged to recognize these heavy, yet important issues that are effecting campuses across the nation. Through Theatre for Development …

Toxicity And Cosmesis Outcomes For Single Fraction Intra-Operative Electron Radiotherapy (Ioert) For Breast Cancer, Monica Hanna, Robert Ash, Wesley Babaran, Michele M. Carpenter, Afshin Forouzannia, Jay K. Harness, Brian Kaltenecker, Snehith Maddula, Venita Williams, Lawrence Wagman

Student Scholar Symposium Abstracts and Posters

Background: Adjuvant radiation therapy is proven to reduce local recurrence in patients with early stage breast cancer. To reduce toxicity, improve geographic accuracy, and reduce treatment time, IOERT can be utilized as an alternative to external beam radiation therapy. The study’s objective was to determine the short term toxicity and cosmesis profile of single fraction IOERT given as definitive treatment in a community setting. Materials and Methods: From Mar 2012 to Jul 2014, 84 patients (3 bilateral), ages 45-91 y.o. with stage 0-II were treated with IOERT (Mobetron, IntraOp Medical, Sunnyvale, CA). A single 21 Gy fraction was administered to …

Assessment Of The Effects Of Caffeine, Gallic Acid, And Epigallocatechin-3-Gallate On Cell Inhibition, Pim-3 And E. Cadherin Protein Levels In Two Lines Of Pancreatic Cancer Cells, Lena Haddad, Melissa Rowland-Goldsmith

Student Scholar Symposium Abstracts and Posters

According to the American Cancer Society, pancreatic cancer is currently the fourth leading cause of cancer related deaths in the United States. In addition to being an exceptionally aggressive form of cancer, it is particularly difficult to treat because it is usually diagnosed in late stages after the onset of metastasis (1). Consequently, the current treatments used, including chemotherapy and radiation, have been rendered ineffective (2). As a result, focus has been placed on using dietary alternatives which are known to possess chemopreventive properties (3). Previous studies have indicated that Gallic acid (an important phytochemical in pomegranates) and Epigallocatechin-3-Gallate (the …

Molecular Insights Into Prostate Field Cancerization: Telomere Length, Egr-­‐1 Expression, And Regulation Of Mic-­‐1, Pdgf-­‐A, And Fas, Emily Frisch, Kristin Gabriel, Marco Bisoffi

Student Scholar Symposium Abstracts and Posters

The diagnosis of prostate cancer (adenocarcinoma) relies on screening for elevated prostate-specific antigen (PSA) in blood samples and on digital rectal examination (DRE). With high PSA levels and/or abnormal DRE, physicians recommend a biopsy, which often misses the location of the adenocarcinoma and results in false negatives. Previous studies have shown expression of the key transcription factor early growth response 1 (EGR-1), the pro-survival factor macrophage inhibitor cytokine 1 (MIC-1), and the growth stimulatory platelet derived growth factor A (PDGF-A) to be up-regulated in histologically normal tissues 1 centimeter adjacent to prostate adenocarcinomas. We hypothesize that tumors emerge from “field …

Prostate Field Cancerization -- Thinking Outside The Tumor, Dor Shoshan, Marco Bisoffi

Student Scholar Symposium Abstracts and Posters

Prostate field cancerization (or field effect) is characterized by the presence of molecular alterations in histologically normal tissues adjacent to adenocarcinomas. Accordingly, our research indicates deregulated expression of several proteins that define this type of molecular pathology. The scope of the present study was to determine the expression of the key transcription factor and potential marker of field cancerization early growth response 1 (EGR-1) in human prostate tissues derived from prostatectomies and biopsy cores.

EGR-1 was detected by immunofluorescence using a polyclonal anti-human EGR-1 and Alexa Fluor 488-conjugated secondary antibodies. EGR-1 expression was quantitated by determining the pixel count per …

Engineered Plga Nanoparticles For Delivery Of Sirna In Mcf-7 Breast Cancer Cells, Sydney Pong, Samit Shah, Vivek Gupta

Student Scholar Symposium Abstracts and Posters

Small interfering RNAs have been an emerging medical treatment for molecular based diseases as they are capable of gene-specific knockdown. Appropriate and efficient delivery remains one of the biggest challenges in the development of siRNA as an anti-cancer treatment. Nanoparticles containing siRNA were characterized and the efficacy of various peptides in the transfection of the nanoparticles were tested. A gene silencing assay was developed in order to determine the effect of siRNA therapeutics on gene functionality in breast cancer cells.

Cognitive Neurosciene And Its Applications To Contemporary Acting Theory, Ryan Stiffelman

Student Scholar Symposium Abstracts and Posters

I personally believe that anyone can be a performer so long as they have the determination, dedication, and refuse to take no for an answer. There are all kinds of training out there designed to help turn hopeful wannabes into the “stars” that we know them as, however, not every “star” we see has had this kind of training. We often hear of young actors like Jennifer Lawrence hailed for their “natural talent”. Does this mean there’s some kind of birth-right, a specific natural inclination or ability that makes someone a potentially great actor from the start? I will refer …

Comparison Of Tetrad And Degree Of Difference Sensory Testing Methods In Evaluating The Quality Of Flour Tortillas, Kristine Futalan, Criselda Toto, Heather Mcdonald

Student Scholar Symposium Abstracts and Posters

The Tetrad test is a forced choice discrimination method that has recently gained popularity in the sensory evaluation of foods. It has demonstrated superiority over the triangle test both in theory and practice with its proven relatively large statistical power. Tetrad does have one potential drawback, the use of a fourth sample that may cause panelist fatigue. There have been many studies comparing the Tetrad method with other difference tests such as triangle, 3- AFC, and 2- AFC however, there has been little research comparing the Tetrad and Degree of Difference test (DOD). This project compared the precision and power …

How The Presence Of Plastic In The North Pacific Gyre Affects The Growth Of Thalassiosira Through Remote Sensing And Laboratory Replication, Jordynn Brennan, Hesham El-Askary

Student Scholar Symposium Abstracts and Posters

Through the use of remote sensing, we are able to determine the approximate location of the garbage patch in the North Pacific Gyre. Though remote sensing does not penetrate the surface of the ocean, monthly satellite images can be analyzed to determine the rate of growth or rate of decrease of certain parameters, such as atmospheric gases, phytoplankton, and dissolved organic matter. Over the past decade, data from the Goddard Earth Sciences Data and Information Services Center (Giovanni program) has shown a significant increase in dissolved organic matter and chlorophyll a content in the area of the North Pacific Garbage …

Structure-Based Network Analysis Of Activation Mechanisms In The Erbb Family Of Receptor Tyrosine Kinases: The Regulatory Spine Residues Are Global Mediators Of Structural Stability And Allosteric Interactions, Kevin A. James, Gennady M. Verkhivker

Mathematics, Physics, and Computer Science Faculty Articles and Research

The ErbB protein tyrosine kinases are among the most important cell signaling families and mutation-induced modulation of their activity is associated with diverse functions in biological networks and human disease. We have combined molecular dynamics simulations of the ErbB kinases with the protein structure network modeling to characterize the reorganization of the residue interaction networks during conformational equilibrium changes in the normal and oncogenic forms. Structural stability and network analyses have identified local communities integrated around high centrality sites that correspond to the regulatory spine residues. This analysis has provided a quantitative insight to the mechanism of mutation-induced ‘‘superacceptor’’ activity …

Peptide Arrays For Detecting Naphthenic Acids In Oil Sands Process Affected Water, Kamaljit Kaur, Subir Bhattacharjee, Rajesh G. Pillai, Sahar Ahmed, Sarfuddin Azmi

Pharmacy Faculty Articles and Research

Naphthenic acids (NAs) are water-soluble components of petroleum. The characterization and quantification of NAs by analytical methods have proved quite challenging, whilst the toxic effects of these water-soluble compounds on a variety of organisms adversely affecting reproduction and steroid production is becoming apparent. In this study, we report a fluorescence-based competitive binding method for rapid sensing of the presence of NAs using cellulosic peptide array strips as sensors. The peptide array was designed from sequences derived from the estrogen receptor (ER). Several of these peptides were able to detect the presence of NAs at low micromolar (∼5 mg L^{−1 …}

Design Of Randomized Experiments In Networks, Dylan Walker, Lev Muchnik

Business Faculty Articles and Research

Over the last decade, the emergence of pervasive online and digitally enabled environments has created a rich source of detailed data on human behavior. Yet, the promise of big data has recently come under fire for its inability to separate correlation from causation-to derive actionable insights and yield effective policies. Fortunately, the same online platforms on which we interact on a day-to-day basis permit experimentation at large scales, ushering in a new movement toward big experiments. Randomized controlled trials are the heart of the scientific method and when designed correctly provide clean causal inferences that are robust and reproducible. However, …

Improving The Efficacy Of Web-Based Educational Outreach In Ecology, Gregory R. Goldsmith, Andrew D. Fulton, Colin D. Witherill, Javier F. Espeleta

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Scientists are increasingly engaging the web to provide formal and informal science education opportunities. Despite the prolific growth of web-based resources, systematic evaluation and assessment of their efficacy remains limited. We used clickstream analytics, a widely available method for tracking website visitors and their behavior, to evaluate 60,000 visits over three years to an educational website focused on ecology. Visits originating from search engine queries were a small proportion of the traffic, suggesting the need to actively promote websites to drive visitation. However, the number of visits referred to the website per social media post varied depending on the social …

Citrus Notes-Agricultural Advantages Of So. California, Charles C. Chapman

Charles C. Chapman Citrus Speeches

These are notes from a speech given by Mr. Chapman at a banquet at the Pomona Farmer's Club Institute. He details the environmental advantages that Southern California has in relation to the rest of the world.

Citrus Talks-Welcoming Dr. Wickerson (Of U. Of C.), Charles C. Chapman

Charles C. Chapman Citrus Speeches

In this speech Mr. Chapman welcomes Dr. Wickerson of the University of California to a gathering of Farmers in Placentia. He makes some comments about the usefulness of science and technology to horticulture. He also laments the loss of many young men from farming families to employment in the city.

Citrus Notes-The Value Of The Tariff To Citrus, Charles C. Chapman

Charles C. Chapman Citrus Speeches

Mr Chapman talks about the importance of the tariff on foreign-grown citrus fruits to the California citrus industry. He details some of the history of the battle to inact the tariff as well as the threats to it at the time this speech was given. He claims that the tariff is the most important factor in the profitabilty of California citrus fruits and that this in turn provides a major support to the economy of the state.

Citrus Talks-Soils And Subsoils, Charles C. Chapman

Charles C. Chapman Citrus Speeches

Mr. Chapman discusses the cultivation and maintenence of healthy, productive soils for citrus fruit farming. Topics he touches on include soil testing, soil composition, cover crops, fertilizers, drainage, irrigation, aeration and mulching. He emphasizes that neglect of any of these aspects of a growers' soil will lead to unhealthy, diseased trees.

Citrus Notes-Fertilization, Charles C. Chapman

Charles C. Chapman Citrus Speeches

This speech discusses the necessity of fertilizing citrus crops and delves into which kinds and how much per tree. Some of the soil amendments Mr. Chapman discusses are potash, bone meal, nitrogen, nitrate of soda, and phosphoric acid. He also stresses the importance of knowing your orchard.

Citrus Notes-Fertilizers, Charles C. Chapman

Charles C. Chapman Citrus Speeches

Charles C. Chapman discusses experimentation with various fertilizers on his Santa Isabel Ranch in Fullerton and fertilizing methods he discussed with orchardists in Fullerton and Placentia. He mentions trying sheep manure, lime cake, bone meal, commercial fertilizer, wood ashes and a combination of each. He concludes that he cannot discern any difference in his crops based on the type of fertilizer employed. He goes on to say that he feels that there is already much plant food in soil and that aeration and frequent cultivation are the key to utilize these nutrients.

Citrus Talks-The Orange, Charles C. Chapman

Charles C. Chapman Citrus Speeches

This talk examines the citrus industry, delves into various types of citrus such as Valencia Lates, and several varieties of the navel orange. It also covers the nuances of fumigation and dealing with pests such as Fuller's Rose Beetle and the red spider. Production, costs, and marketing are also discussed.

Citrus Talks-The Value Of The Farmer, Charles C. Chapman

Charles C. Chapman Citrus Speeches

This talk explores how farmers are sometimes preyed upon by big city folks. It also praises farmers as being strong, dependable, and resourceful and how these qualities add to the integrity of cities when farmers move there.

Citrus Talks-The Citrus Industry, Charles C. Chapman

Charles C. Chapman Citrus Speeches

While Orange County, California, is one of the smallest in area, it supports a more diversified farming industry than any other, due to the climate, soil, abundant water sources, and the progresses in technology for handling, packing, and shipping the fruit.

Chapman Speech Index, Charles C. Chapman

Charles C. Chapman Citrus Speeches

Index for Speeches indexes the following speeches: Citrus, Clubs & Organizations, College, Dedications, Funerals, Masonic, Memorial Addresses, Miscellaneous, Politics, Sermons (Applied Christianity, The Bible, The Church, Conventions, Doctrinal, Jesus, Miscellaneous), Miracles & Parables, Temperance, Y.M.C.A.

The Non-Canonical Hydroxylase Structure Of Yfcm Reveals A Metal Ion-Coordination Motif Required For Ef-P Hydroxylation, Kan Kobayashi, Assaf Katz, Andrei Rajkovic, Ryohei Ishii, Owen E. Branson, Michael A. Freitas, Ryuichiro Ishitani, Michael Ibba, Osamu Nureki

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

EF-P is a bacterial tRNA-mimic protein, which accelerates the ribosome-catalyzed polymerization of poly-prolines. In Escherichia coli, EF-P is post-translationally modified on a conserved lysine residue. The post-translational modification is performed in a two-step reaction involving the addition of a β-lysine moiety and the subsequent hydroxylation, catalyzed by PoxA and YfcM, respectively. The β-lysine moiety was previously shown to enhance the rate of poly-proline synthesis, but the role of the hydroxylation is poorly understood. We solved the crystal structure of YfcM and performed functional analyses to determine the hydroxylation mechanism. In addition, YfcM appears to be structurally distinct from any …

Mapping Regions Of Rnf168 Required For Its Degradation By Icp0, Andrea Cyr, Matthew Weitzman

e-Research: A Journal of Undergraduate Work

Viruses establish infection by overtaking host cell processes and developing mechanisms that promote viral replication. Herpes simplex virus undergoes lytic and latent cycles of infection throughout the lifespan of its host. The viral genome is transcriptionally silent during latency, but viral proteins are produced upon reactivation. Herpes simplex virus type 1 encodes the ICP0 protein, an E3 ubiquitin ligase required for reactivation from latency of the infectious virus. The immediate-early protein ICP0 regulates the herpes simplex virus by activating viral gene expression thereby initiating lytic infection. Cellular proteins are degraded by ICP0, promoting the virus to enter the lytic cycle. …

Sleep Patterns In Collegiate Dancers, Beth Nicks, Jacque Price

e-Research: A Journal of Undergraduate Work

As college dance majors, the investigators have witnessed firsthand the effects that sleep and the lack thereof can have on the performance quality of University level dancing. It has been shown that lack of sleep can compromise nearly every physiological process such as memory, coordination, metabolism, and new learning (Wozny 2008). Studies on disruptions of the human circadian rhythms have shown that athletes involved in endeavors where sleep is restricted or totally deprived have severe consequences on behavior and performance. (Edwards 2007). However, the environment of college dance programs is different than athletics due to there being no off season. …