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De Novo Sequencing And Analysis Of Salvia Hispanica Transcriptome And Identification Of Genes Involved In The Biosynthesis Of Secondary Metabolites, James Wimberley
De Novo Sequencing And Analysis Of Salvia Hispanica Transcriptome And Identification Of Genes Involved In The Biosynthesis Of Secondary Metabolites, James Wimberley
Computational and Data Sciences (MS) Theses
Salvia hispanica L. (commonly known as chia) is gaining popularity worldwide and specially in US as a healthy oil and food supplement for human and animal consumption due to its favorable oil composition, and high protein, fiber, and antioxidant contents. Despite these benefits and its growing public demand, very limited gene sequence information is currently available in public databases. In this project, we generated 90 million high quality 150 bp paired-end sequences from the chia leaf and root tissues. The sequences were de novo assembled into 103,367 contigs with average length of 1,445 bp. The resulted assembly represented 92.2% …