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Full-Text Articles in Life Sciences
Research Progress Of Triplex Nucleic Acid In Food Safety Detection, Sun Hai-Yan, Lei Yan-Li, Chen Wei-Ju, Quan Ke
Research Progress Of Triplex Nucleic Acid In Food Safety Detection, Sun Hai-Yan, Lei Yan-Li, Chen Wei-Ju, Quan Ke
Food and Machinery
This paper reviews the structure and stability of triplex nucleic acid, the progress of triplex nucleic acid-based fluorescence, electrochemical, colorimetric platform in detecting food borne hazards, and discusses the challenges and future development prospect of triplex nucleic acid-based detection technology in food safety field.
Study On The Stability Of Lipase Of Terminally Cyclized Thermomyces Lanuginosus, Song Wen, Wu Xia-Xia, Lou Hai-Wei, Fang Hui-Min
Study On The Stability Of Lipase Of Terminally Cyclized Thermomyces Lanuginosus, Song Wen, Wu Xia-Xia, Lou Hai-Wei, Fang Hui-Min
Food and Machinery
Objective: To obtain industrial standard lipase with high activity and stability, Butelase 1 ligase was used to connect the N- and C-termini of Thermomyces lanuginosus lipase (TLL), and then the protein characteristics, including heat stability, protease resistance, and enzymatic kinetics were analyzed. Methods: His6 affinity column was used to purify recombinant Butelase 1 (rButelase 1) and TLL (rTLL) from mammalian cells and E. co- li; rButelase 1 to was utilized connect the N- and C-termini of rTLL to generate circular rTLL (cTLL); the enzymatic activity, the heat stability, the protease resistance, the resistance to heat-induced precipitation, and …
Effect Of Compound Thickener On The Quality Of Red Jujube And Aloe Low-Sugar Jam, Lin Zhi-Rong
Effect Of Compound Thickener On The Quality Of Red Jujube And Aloe Low-Sugar Jam, Lin Zhi-Rong
Food and Machinery
Objective: This study aimed to solve the poor viscosity of red jujube and aloe low-sugar jam. Methods: Using CMC-Na and AGAR as thickeners, the effects of compound thickeners on texture properties and sensory quality of red jujube and aloe low-sugar jam under different storage time were investigated. The technological parameters of jam were optimized by orthogonal test and rheological test. Results: When the ratio of CMC-Na and AGAR in the compound thickener was 7∶3, no significant difference in hardness and viscosity were found between fresh jam and preserved 90 d jam, and the jam was delicate and …
Preparation, Selenium Content And Stability Of Antihypertensive Peptides From Selenium-Enriched Moringa Oleifera Seed Protein By Enzymatic Hydrolysis, Chen Bing-Bing, Yang Yi, Li Jia-Yi, Jin Chang-Yan, Cheng Bin-Pei, Deng Yong-Qi, Lin Bi-Min, Liang Dong, Tang De-Jian, Meng Li, Miao Jian-Yin
Preparation, Selenium Content And Stability Of Antihypertensive Peptides From Selenium-Enriched Moringa Oleifera Seed Protein By Enzymatic Hydrolysis, Chen Bing-Bing, Yang Yi, Li Jia-Yi, Jin Chang-Yan, Cheng Bin-Pei, Deng Yong-Qi, Lin Bi-Min, Liang Dong, Tang De-Jian, Meng Li, Miao Jian-Yin
Food and Machinery
Objective: To develop selenium-enriched (Se-enriched) Moringa oleifera seed protein resources. Methods: Using Se-enriched Moringa oleifera seed protein powder as raw material, the enzymatic hydrolysis conditions of Se-enriched Moringa oleifera seed protein antihypertensive peptides from Se-enriched Moringa oleifera seed protein were obtained by single factor and response surface optimization, and the ACE inhibitory activity, Se content and stability of the optimal enzymatic hydrolysate were analyzed. Results: The optimal enzymatic hydrolysis conditions of Se-enriched Moringa oleifera were as follows: enzymatic hydrolysis time 3 h, temperature 34 ℃, pH 8, concentration of substrate 7%, enzyme to substrate ratio 0.3%. Under …
Antioxidant And Stability Of Polysaccharides From Purple Sweet Potato By Fractional Alcohol Precipitation, Lin Jun, Li Gao-Yang, Huang Fan, Xie Qiu-Tao, Yuan Hong-Yan
Antioxidant And Stability Of Polysaccharides From Purple Sweet Potato By Fractional Alcohol Precipitation, Lin Jun, Li Gao-Yang, Huang Fan, Xie Qiu-Tao, Yuan Hong-Yan
Food and Machinery
bjective:This study aimed to explore the composition, antioxidant activity and stability of purple potato polysaccharides and improve the efficiency of separation purification.Methods:The free radical scavenging capacity, antioxidant capacity and chemical stability of the crude polysaccharides from purple sweet potato were compared and analyzed by fractional alcohol precipitation.Results:The contents of polysaccharide, protein, sulfate group and uronic acid in the three fractions were in the order of PPSP-80% ＞ PPSP-60% ＞ PPSP-40%. Among them, PPSP-80% fraction had the strongest scavenging ability on DPPH, ABTS and superoxide anion free radicals, while PPSP-60% fraction had the strongest scavenging ability on …
Preliminary Study Of Encapsulation To Improve Stability Of Aldh Of Wickerhamomyces Anomalus, Chen Geng, Ni Yu-Jun, Zhong Min, Xu Li-Na, Guo Wei-Dan
Preliminary Study Of Encapsulation To Improve Stability Of Aldh Of Wickerhamomyces Anomalus, Chen Geng, Ni Yu-Jun, Zhong Min, Xu Li-Na, Guo Wei-Dan
Food and Machinery
Objective: This study aimed to improve the stability Wickerhamomyces anomalus aldehyde dehydrogenase in cold storage and gastrointestinal environment, so as to develop products that reduce active aldehydes in gastrointestinal tract. Methods: ALDH was extracted from 6 Wickerhamomyces anomalus strains with the highest activity of aldehyde dehydrogenase (ALDH), and encapsulated by sodium alginate and calcium chloride method. The stability of ALDH by embedding was studied in cold storage and simulated gastrointestinal environment. Results: The specific activity of ALDH produced by W. anomalus J3, J7, J8, J9, J12 and J18 ranged from 86.77 U/mg to 482.14 U/mg, and that of J9 was …