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Identification Of Proteins Involved In Chloroplast Dna Replication, Matthew G. Lassen
Identification Of Proteins Involved In Chloroplast Dna Replication, Matthew G. Lassen
Theses and Dissertations
Chapter 1
Chloroplast nucleoids (ct-nucleoids) are DNA/protein complexes involved in compacting the chloroplast genome, and may play a role in regulating DNA replication. Ct-nucleoids were isolated from young soybean plants and separated by 2-D gel electrophoresis. Gel spots were excised and analyzed by MALDI-ToF mass spectrometry, resulting in several protein identifications. The proteins identified all have functions unrelated to DNA replication. While some of these proteins may be due to contamination, it is possible that some of these proteins are dual-functional, playing direct roles in the regulation of DNA replication.
Chapter 2
A 28 kDa soybean protein was isolated by …