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Full-Text Articles in Life Sciences
Mapping Auditory And Vestibular Response Neurons In The Optic Tectum Of Larval Zebrafish, Erika Marks, Arminda Suli
Mapping Auditory And Vestibular Response Neurons In The Optic Tectum Of Larval Zebrafish, Erika Marks, Arminda Suli
Undergraduate Honors Theses
Cellular mapping of the sensory receptive fields of brain structures is a critical step in understanding function and dysfunction in development. Deficits in the ability to receive, process and integrate the senses leads to difficulties recognizing and responding appropriately to stimuli. To understand how the senses are integrated, it is first necessary to map the neurons receptive to inputs from individual senses. The optic tectum of zebrafish is a structure known for its homology to the superior colliculus of mammals and is a center of multisensory integration – including visual, auditory, and somatosensory stimuli. However, the microcircuitry of this important …
Identifying And Knocking Out Non-Visual Opsins In The Optic Tectum Of Zebrafish Larvae, Sarah Jarrett
Identifying And Knocking Out Non-Visual Opsins In The Optic Tectum Of Zebrafish Larvae, Sarah Jarrett
Undergraduate Honors Theses
Deep brain photoreceptors (DBPs) are non-visual photoreceptors found in the brains of non-mammalian vertebrates like zebrafish. While using optogenetic techniques in our lab to photo-activate the ear (by expressing the light-gated channel: channelrhodopsin) in efforts of identifying optic tectum (OT) neurons that respond to and integrate auditory and visual stimuli, we found that violet light was able to elicit OT neuronal activity. OT activity was also detected in cases when visual and auditory stimulation was not possible due to removal of the eyes and absence of the exogenously expressed channelrhodopsin, respectively. Analyzing OT single-cell sequencing data of 7day post fertilization …
Governing Dynamics Of Divalent Copper Binding By Influenza A Matrix Protein 2 His37 Imidazole, Kelly Lewis Mcguire
Governing Dynamics Of Divalent Copper Binding By Influenza A Matrix Protein 2 His37 Imidazole, Kelly Lewis Mcguire
Theses and Dissertations
Influenza A is involved in hundreds of thousands of deaths globally every year resulting from viral infection-related complications. Previous efforts to subdue the virus by preventing proper function of wild-type (WT) neuraminidase (N), and M2 proteins using oseltamivir and amantadine (AMT) or rimantadine (RMT), respectively, exhibited success initially. Over time, these drugs began exhibiting mixed success as the virus developed drug resistance. M2 is a proton channel responsible for the acidification of the viral interior which facilitates release of the viral RNA into the host. M2 has a His37-tetrad that is the selective filter for protons. This protein has been …
A Crispr/Cas9 Tissue Specific Forward Genetic Screening Method In Danio Rerio, Joshua Don Yates
A Crispr/Cas9 Tissue Specific Forward Genetic Screening Method In Danio Rerio, Joshua Don Yates
Theses and Dissertations
Many of the cutting-edge innovations on the CRISPR/Cas9 system involve the use of complex sgRNA libraries. For example, such libraries have recently been used in functional genomics applications to screen for specific genes in both cell lines and living organisms, as well as in subcellular imaging to fluorescently label chromatin in living cells. However, chemically synthesizing customized libraries can be cost prohibitive, requires precise genomic information, and can takes several weeks. We developed a rapid and efficient enzymatic method to generate sgRNA libraries from arbitrary DNA substrates, significantly reducing the cost of library generation. A type IIS restriction enzyme binding …
Identifying The 5’End Of The Camta1 Genes In Zebrafish, Morgan Fronk
Identifying The 5’End Of The Camta1 Genes In Zebrafish, Morgan Fronk
Undergraduate Honors Theses
Congenital heart diseases (CHDs) are a significant cause of infant death and are frequently caused by mutations in transcription factors. Camta1 (calmodulin binding transcription activator 1) is a transcription factor that has been proposed as a modulator in embryonic heart development and a possible cause of CHDs. The only other known member of its family in vertebrates is involved in activating a hypertrophy gene program in adult heart failure. Unlike camta2, camta1 is expressed in the embryonic heart during heart looping. However, few studies have been done on camta1. In zebrafish, there are two camta1 ohnologs (homologs created through …