Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
Light And Electron Microscopy Of Resistant And Susceptible Alfalfa Roots Infected By Meloidogyne Hapla, Bernard Al Tait
Light And Electron Microscopy Of Resistant And Susceptible Alfalfa Roots Infected By Meloidogyne Hapla, Bernard Al Tait
Theses and Dissertations
Resistant (65-410 and 65-393) and susceptible (breeders Lahontan) alfalfa seedlings were infected with the Northern root-knot nematode, Meloidogyne hapla. Infected roots were fixed, stained, and embedded in plastic for ultrastructural investigations. Sections of plastic embedded tissue, cut at 0.5-1.5 microns for light microscopy investigations, were stained with various histochemical stains to determine the chemical composition of the nematode-altered tissues. In the susceptible cultivar, giant cells were formed by enlargement of nematode-stimulated cells without cell wall lysis. The giant cells became multinucleate by karyonkinesis without cytokinesis. Proplastids in the giant cells became structurally altered to form tubular complexes. The resistant plants …