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Full-Text Articles in Life Sciences
Fgf4 Induced Wnt5a Gradient In The Limb Bud Mediates Mesenchymal Cell Directed Migration And Division, John C. Allen
Fgf4 Induced Wnt5a Gradient In The Limb Bud Mediates Mesenchymal Cell Directed Migration And Division, John C. Allen
Theses and Dissertations
The AER has a vital role in directing embryonic limb development. Several models have been developed that attempt to explain how the AER directs limb development, but none of them are fully supported by existing data. I provide evidence that FGFs secreted from the AER induce a gradient of Wnt5a. I also demonstrate that limb mesenchyme grows toward increasing concentrations of Wnt5a. We hypothesize that the changing shape of the AER is critical for patterning the limb along the proximal to distal axis. To better understand the pathway through which Wnt5a elicits its effects, we have performed various genetic studies. …
Molecular And Cellular Mechanisms Whereby The Apical Ectodermal Ridge (Aer), Via Wnt5a, Mediates Directional Migration Of The Adjacent Mesenchyme During Vertebrate Limb Development, Kate E. Kmetzsch
Theses and Dissertations
The vertebrate embryonic limb is a key model in elucidating the genetic basis underlying the three dimensional morphogenesis of structures. Despite the wealth of insights that have been generated from this model, many long-standing questions remain. For example, it has been known for over 70 years that the apical ectodermal ridge (AER) of the embryonic limb is essential for distal outgrowth and patterning of the adjacent limb mesenchyme. The mechanisms whereby the AER does accomplish outgrowth and patterning are still poorly understood. We propose that secreted FGFs from the AER activate Wnt5a expression in gradient fashion, which in turn provides …
Wnt5a Signaling Independently Of The Planar Cell Polarity Pathway Resulting In Convergent Extension And Neural Tube Closure During Vertebrate Development, Jared James Barrott
Wnt5a Signaling Independently Of The Planar Cell Polarity Pathway Resulting In Convergent Extension And Neural Tube Closure During Vertebrate Development, Jared James Barrott
Theses and Dissertations
Vertebrate development is regulated by cellular communication by mechanisms of cell fate and cell behavior. These crucial mechanisms are regulated by cellular signaling and in the case of cell fate, cellular signaling results in transcription of developmentally important genes. Communication between cells can also result in regulation of cell behavior by acting on cytoskeletal elements rather than nuclear factors. One of the cellular signals that regulate both cell fate and cell behavior is the family of Wnt signaling molecules. Wnt5a is one of 19 Wnt molecules and has been previously demonstrated to play critical roles in many important processes in …
Determining The Role Of Wnt5a Signaling In Embryonic Limb Outgrowth Via Clonal Analysis, Whitney Herrod Sowby
Determining The Role Of Wnt5a Signaling In Embryonic Limb Outgrowth Via Clonal Analysis, Whitney Herrod Sowby
Theses and Dissertations
The exact mechanisms that regulate limb outgrowth the mouse embryo are unknown. Although there are several models, we favor a hypothesis where cells become polarized by signals secreted from the AER which orient their cell migration and/or divisions causing limb outgrowth. Clonal analysis has provided a mechanism to study cell behavior. We have generated a targeting construct containing the Fgf8 inhibitor, Sprouty2, in order to generate mutant clones for behavioral analyses in the limb. In order to more effectively study clonal behavior we report the modification of a novel clonal analysis approach, exo-utero surgery. We have modified, enhanced and proven …