Life Sciences Commons^™

Identifying Topographic Controls Of Terrestrial Vegetation Using Remote Sensing Data In A Semiarid Mountain Watershed, Idaho, Usa, Ricci Loughridge

Boise State University Theses and Dissertations

Global climate change is a significant research focus area in contemporary Earth science. Changes in climatic patterns have already resulted in shifting energy flows with associated changes in hydrologic and ecologic systems. More specifically, changes in vegetation distribution and abundance are one of the most visible and potentially significant effects of a changing climatic regime. However, to monitor and predict future changes in vegetation, the initial conditions must be well characterized. This thesis examines the distribution of vegetation in a semiarid mountain watershed in three important ways: (1) quantifying the factors affecting the distribution of broad classes of vegetation at …

Traffic Noise Decreases Body Condition And Stopover Efficiency Of Migrating Songbirds, Heidi Elise Ware

Boise State University Theses and Dissertations

Numerous studies document impacts of roads on wildlife, and suggest traffic noise as a primary cause of population declines near roads. For migratory birds faced with increasingly human-altered habitats, noise may pose a serious threat. Using an array of speakers, we applied traffic noise to a roadless landscape, directly testing the effect of noise alone on an entire songbird community. Focusing on individuals that stayed despite the noise, we demonstrate that songbirds show a near halving of ability to gain body condition when exposed to traffic noise during migratory stopover. This marked degradation in stopover efficiency may help explain dramatic …

Ciliates Of Sagebrush-Steppe Soils Of Southwestern Idaho: Species Richness And Its Relationship To Changed Soil Variables After Wildfire Events, Laura G. Wendell

Boise State University Theses and Dissertations

I conducted a pilot study to examine species richness of terrestrial ciliates associated with big sagebrush habitat (Artemisia tridentata) in southwestern Idaho, USA. As wildland fires are a naturally occurring disturbance in this area, soil variables associated with these fires (% C, % N, pH, soil texture, and % clay) were measured at three sites, both in burned and unburned areas. These soil characteristics were compared to corresponding ciliate diversity and ratios of r-selected colpodeans to K-selected polyhymenophorans (C/P) in non-flooded Petri dish preparations. A total of 85 ciliate taxa were observed across the three sites, including …

Evolutionary Search For Models Of Planarian Regeneration Using Experimental Data, Marianna Viktorovna Budnikova

Boise State University Theses and Dissertations

The ability of science to produce experimental data greatly surpasses our current ability to effectively visualize, conceptualize, and integrate the vast volumes of available data into a unified understanding of how complex biological systems work. This inability is a hindrance to scientific progress, and is particularly daunting when one considers multidimensional and shape-based observations as in the field of regenerative biology. For example, for at least the last 200 years, scientists have been interested in the exceptional ability of Planaria to regenerate lost tissues from damage, and there is a large amount of experimental data available on this organism. However, …

The Dilemma Of Foraging Herbivores: Dealing With Food And Fear, Clare Mcarthur, Peter B. Banks, Rudy Boonstra, Jennifer Sorensen Forbey

Biology Faculty Publications and Presentations

For foraging herbivores, both food quality and predation risk vary across the landscape. Animals should avoid low-quality food patches in favour of high-quality ones, and seek safe patches while avoiding risky ones. Herbivores often face the foraging dilemma, however, of choosing between high-quality food in risky places or low-quality food in safe places. Here, we explore how and why the interaction between food quality and predation risk affects foraging decisions of mammalian herbivores, focusing on browsers confronting plant toxins in a landscape of fear. We draw together themes of plant–herbivore and predator–prey interactions, and the roles of animal ecophysiology, behaviour …

Construction And Characterization Of Non-Toxic Bacterial Enterotoxins As Vaccine Adjuvants, Lavanya Vempati

Boise State University Theses and Dissertations

The development of adjuvants that can promote the delivery of purified subunit vaccines by mucosal routes, such as the nose or the mouth, is recognized as a top priority for vaccine research. The bacterial enterotoxins; cholera toxin (CT) and E.coli heat-labile toxin (LTI), have long been recognized as powerful adjuvants with the ability to stimulate specific immune responses to co-administered antigens when delivered to mucosal surfaces. Shiga toxin 1 (ST1) and pertussis toxin (PT) are structurally homologous bacterial toxins secreted by Escherichia coli 0157:H7 and Bordetella pertussis respectively. ST1 and PT also have reported adjuvant activity but it is less …

Redescription Of Atopospira Galeata (Kahl, 1927) Nov. Comb. And A. Violacea (Kahl, 1926) Nov. Comb. With Redefinition Of Atopospira Jankowski, 1964 Nov. Stat. And Brachonella Jankowski, 1964 (Ciliophora, Armophorida), William A. Bourland, Laura Wendell

Biology Faculty Publications and Presentations

The taxonomy of the Metopidae (Ciliophora, Armophorida) remains poorly understood since most of its members have not been studied by modern morphologic and molecular methods. Recent molecular investigations have indicated that the two most species-rich genera, Metopus and Brachonella, are likely nonmonophyletic with at least one well-supported 18S rDNA clade comprised of a species from each of these genera (Brachonella galeata and Metopus violaceus). We investigated these two species with silver impregnation and scanning electron microscopy. Both taxa share important morphologic characteristics not described in other species of Metopus or Brachonella. These synapomorphies include: (1) a …

Mechanistic And Substrate Specificity Studies On Burkholderia Mallei Quorum Sensing Signal Synthesis Enzyme, Aubrey N. Montebello

Boise State University Theses and Dissertations

Gram-negative bacteria use acyl-homoserine lactone (AHL) based quorum sensing (QS) to regulate the expression of genes that give the bacteria a selective advantage over host defenses and antibiotic treatment. Burkholderia mallei is an antibiotic resistant pathogen that causes Glanders disease. B. mallei BmaI1 AHL-synthase uses octanoyl-Acyl Carrier Protein (C8ACP) and S-adenosyl-L-methionine (SAM) to synthesize the AHL, octanoyl-homoserine lactone (C8HSL). Inhibiting AHL-synthases has been difficult because mechanistic and substrate specificity details for these enzymes are not well understood. Our goal was to determine how BmaI1 activity and enzymatic mechanism changes with nonspecific, variable acyl chain acyl-ACP substrates. We found that catalytic …

Exploration Into The Cellular Effects Of Aziridinomitosenes, Christopher M. Mallory

Boise State University Theses and Dissertations

Aziridinomitosenes (AZMs) are organic compounds structurally related to the mitomycins, a class of anti-tumor agents and antibiotics. The cytotoxicity of the mitomycins is correlated to their ability to covalently link complimentary strands of DNA, forming DNA interstrand cross-links (ICLs). Currently, there has been limited investigation into the biological activity of AZMs, likely due to difficulties in their synthesis. Our lab has synthesized and evaluated the cellular effects of two AZMs, (1S, 2S)-6-desmethyl(methylaziridino)mitosene (H/H-AZM) and (1S, 2S)-6-methyl(methylaziridino)mitosene (Me/H-AZM). We hypothesize that AZMs exhibit their cytotoxicity and cellular effects following a similar pathway to that of mitomycin C (MC) including …

The Effect Of Attentional Focus Instructions On Golf Swing Performance In Recreational Golfers, Jeffrey D. Eggleston

Boise State University Theses and Dissertations

Previous research has addressed the effectiveness of attentional focus instructions in improving golf performance with a single training session. The purpose of the current study was to investigate the effect of external (EF) attentional focus instructions on recreational golfers’ performance over a distributed training period and extended retention interval. Performance was measured by club head velocity (CHV) and X-factor as both have been correlated with greater performance. The current study extends the work of An, Wulf, and Kim (2013), by increasing the training period and retention interval. It was hypothesized EF group would have greater CHV and X-factor measurements during …

Effect Of Weight Loss Training Protocol Using Two Different Treadmills For Obese Individuals, Kristi Lynn Bercier

Boise State University Theses and Dissertations

The AlterG Anti-Gravity Treadmill allows individuals to walk at reduced body weight by using lower body positive pressure (LBPP). PURPOSE: The purpose of this study was to discern if the AlterG Anti-Gravity Treadmill was an effective tool to use in a weight loss walking program with obese individuals when compared to walking on a traditional treadmill. METHODS: Fifteen male (n = 3) and female (n = 12) obese participants, aged 18-55 years old with an average body mass index [BMI] ≥ 30 kg/m²), were randomly assigned to the AlterG treadmill (AlterG; n = 6) and traditional treadmill (TT; …

Quantifying Habitat Relationships Of Songbirds In Quaking Aspen (Populus Tremuloides) And Other Montane Communities Of The Jarbidge Mountains, Nevada, Kevin Patrick Glueckert

Boise State University Theses and Dissertations

Quaking aspen (Populus tremuloides) woodlands are expected to be sensitive to climate change, and have declined in parts of the West. Great Basin mountain ranges may be near the limits of aspen’s climatic threshold, in terms of temperature and aridity, and thus are particularly vulnerable to climate change. Birds associating with aspen are likely to undergo regional population fluctuations and changes in distribution as a result of changes in aspen availability or distribution. Thus, understanding the habitat relationships of avian communities in aspen and other montane cover types is important for tracking the impacts of future landscape change. …

Behavioral Responses Of Burrowing Owls To Experimental Clutch Manipulations: An Investigation Of Conspecific Brood Parasitism And Laying Determinacy, Jamie Lynn Groves

Boise State University Theses and Dissertations

During 2012-2013, I investigated the behavioral responses of western burrowing owls (Athene cunicularia hypugaea) in southwestern Idaho to experimental conspecific brood parasitism to determine if they were capable of egg discrimination, and if they would accept or reject a parasitic egg introduced into their nest. I experimentally added one egg derived from source nests to host nests either during the egg-laying period or just after adult females initiated incubation and examined acceptance or rejection of the parasitic egg in relation to control nests where eggs were handled but not exchanged. Burrowing owl responses to parasitic eggs varied with …

Osteoblast Oncostatin M Signaling In Modeled Spaceflight, Jacob Goyden

Boise State University Theses and Dissertations

Bone deterioration is a challenge in long-term spaceflight with significant connections to terrestrial disuse bone loss. Prolonged unloading and radiation exposure, defining characteristics of space travel, have both been associated with changes in inflammatory signaling via IL-6 class cytokines in bone. While there is also some evidence for perturbed IL-6 class signaling in spaceflight, there has been scant examination of the connections between free fall, radiation, and inflammatory stimuli in bone. Our lab and others have shown that the IL-6 class cytokine oncostatin M (OSM) is an important regulator of bone remodeling. We hypothesize that spaceflight alters osteoblast OSM signaling, …

An Observation Of Apparent Teaching Behavior In The Pallid Bat, Antrozous Pallidus, Jessie P. Bunkley, Jesse R. Barber

Biology Faculty Publications and Presentations

During a laboratory study of pallid bat (Antrozous pallidus) hunting behavior, we observed an interaction wherein an adult female appeared to aid a juvenile male in learning a novel foraging task. This single observation adheres to the 3 requirements of teaching outlined by Caro and Hauser (1992). A female bat experienced with a hunting task modified her behavior in the presence of a naïve observing male, resulting in a cost of reduced food availability to the female when she was hungry, while directing the male to food resources and accelerating his learning of a foraging task. …

Movements Of A Juvenile Crowned Eagle (Harpyhaliaetus Coronatus) Tracked By Satellite Telemetry In Central Argentina, Vicente Urios, Maria Pilar Donat-Torres, Marc J. Bechard, Miguel Ferrer

Biology Faculty Publications and Presentations

Background: A juvenile Crowned Eagle was tagged at its nest with a satellite transmitter. The Crowned Eagle (Harpyhaliaetus coronatus) is one of the most unknown raptor species from the American continent. Their current distribution ranges from central Brazil to central Argentina, with a total population of 350–1500 individuals across this large area, being thus largely fragmented.

Results: During the three years of tracking the bird concentrated its movements in a range spanning for 12845 km², but concentrating mainly in four smaller areas accounting for 3073 km². The locations were recorded mainly over shrubland habitats …

Diet Of A Cryptic But Widespread Invader, The Oriental Weatherfish (Misgurnus Anguillicaudatus) In Idaho, Usa, Alexander N. Urquhart, Peter Koetsier

Biology Faculty Publications and Presentations

The oriental weatherfish (Misgurnus anguillicaudatus) is invasive to 13 U.S. states and at least 9 other countries. Although this cryptic fish species is widespread, very little is known regarding its ecology. We used baited minnow traps to collect 237 individuals from water bodies connected to the Boise River, Idaho. To determine the diet of the fish within this invasive population, we dissected and examined stomach contents of the sampled fish. Most of the stomachs (158 of 237) were empty, and 42 contained only the trap bait. However, 37 contained natural food items. Gravimetric analysis, frequency of occurrence, and …

Factors Associated With Flammulated Owl And Northern Saw-Whet Owl Occupancy In Southern Idaho, Micah N. Scholer, Matthias Leu, James R. Belthoff

Biology Faculty Publications and Presentations

Spatially explicit models depicting species occupancy offer a useful conservation tool for land managers. Using occurrence data collected in 2009 and 2010 from the Boise National Forest, Idaho, we developed distribution models for Flammulated Owls (Psiloscops flammeolus) and Northern Saw-whet Owls (Aegolius acadicus) to explore associations between habitat factors and owl occupancy. We then spatially applied these models in a Geographic Information System. We considered land cover and topographic variables at three spatial scales: 0.4-km, 1-km, or 3-km-radius plots centered on point-count locations (n  =  150) with resolution of land covers at 30 m. Flammulated …

Population Response To Climate Change: Wintering Strategy Has Carryover Effects On The Timing Of Nest Initiation And Mate Choice In A Partial Migrant, The American Kestrel (Falco Sparverius), Alexandra Marie Anderson

Boise State University Theses and Dissertations

The ability of a population to respond to climate change will depend on phenotypic plasticity, adaptation, or both. Bird populations have already responded to warming temperatures by shifting their distributions, adjusting migration distance and timing, and breeding earlier. A population of American kestrels (Falco sparverius) in southwestern Idaho has advanced its nesting by approximately 30 days, on average, in the last 30 years and this has been correlated with changes in winter climate. The mechanisms allowing for this shift, however, are not clearly understood. I investigated if assortative mating of wintering kestrels and non-wintering kestrels is accompanying to …

Influence Of Wildfire Disturbance And Post-Fire Seeding On Vegetation And Insects In Sagebrush Habitats, Ashley T. Rohde

Boise State University Theses and Dissertations

Disturbance events alter community composition and structure because of differences in resistance and resilience of individual taxa, changes in habitat resulting in colonization by new taxa and alteration of biotic interaction patterns. Recent changes in disturbance types, frequencies and intensities caused by anthropogenic activities may further alter community composition and structure if these disturbances exceed the tolerances or adaptations of some taxa. In sagebrush steppe habitats of the western United States, wildfire is the current dominant disturbance type, burning millions of hectares annually. Further, up to 90% of sagebrush-steppe ecosystems are affected by anthropogenic influences such as invasive species. Post-fire …

Making More Sensitive Dna Sensors Using Gold Nanoparticles And Dna-Based Amplification Networks, Herbert M. Huttanus

Boise State University Theses and Dissertations

This study focuses on the use of gold nanoparticles with DNA-based signal amplification as a detection method for low concentrations of DNA bio-markers. Biotechnology is a rapidly evolving field with primarily medical applications. Early detection is a challenging process for some cancers and other diseases yet is so critical to successful treatment. Increasingly sensitive detection techniques are being developed, but the current gold standard for detecting nucleotide biomarkers at low concentrations is polymerase chain reaction. While this technique is sensitive, it requires the use of active enzymes, a thermocycler, and trained personnel working in a clean environment, and is thus …

The First Successful Use Of A Low Stringency Familial Match In A French Criminal Investigation, Emmanuel Pham-Hoai, Frank Crispino, Greg Hampikian

Biology Faculty Publications and Presentations

We describe how a very simple application of familial searching resolved a decade-old, high-profile rape/murder in France. This was the first use of familial searching in a criminal case using the French STR DNA database, which contains approximately 1,800,000 profiles. When an unknown forensic profile (18 loci) was searched against the French arrestee/offender database using CODIS configured for a low stringency search, a single low stringency match was identified. This profile was attributed to the father of the man suspected to be the source of the semen recovered from the murder victim Elodie Kulik. The identification was confirmed using Y-chromosome …

Immunogenicity Of A West Nile Virus Diii-Cholera Toxin A2/B Chimera After Intranasal Delivery, Juliette K. Tinker, Jie Yan, Reece J. Knippel, Panos Panayiotou, Kenneth A. Cornell

Biology Faculty Publications and Presentations

West Nile virus (WNV) causes potentially fatal neuroinvasive disease and persists at endemic levels in many parts of the world. Despite advances in our understanding of WNV pathogenesis, there remains a significant need for a human vaccine. The domain III (DIII) region of the WNV envelope protein contains epitopes that are the target of neutralizing antibodies. We have constructed a chimeric fusion of the non-toxic cholera toxin (CT) CTA₂/B domains to DIII for investigation as a novel mucosally-delivered WNV vaccine. Purification and assembly of the chimera, as well as receptor-binding and antigen delivery, were verified by western blot, …

Collagen Xiα1 And The Stem Cell Theory Of Cancer, Cody Roell, Kenneth Weekes, Julia Oxford

College of Arts and Sciences Presentations

Cancer stem cell (CSC) theory hypothesizes that heterogeneity within tumors is not a mere consequence of random mutation and clonal evolution, but results from an intrinsic hierarchy of cells. The KeWe cell line was isolated and characterized in Dr. Oxford’s lab. Characterization has included the determination of conditions for maintenance in cell culture for extended periods of time and using different techniques to count the cells to characterize cellular proliferation rates. Collagen XIα1 can be found throughout the body in a variety of places including tendons, skin, ligaments, interstitial tissue, dentin, blood vessels, the cornea, intervertebral discs, muscle, bone, and …

Expediting Drug Discovery: Fast And Accurate Prediction Of Coupling Constants For Nitrogen Heterocycles, Petr Malek

College of Arts and Sciences Presentations

Nitrogen containing heterocyclic drugs that regulate the JAK-STAT pathway have proven therapeutic usage for a variety of disorders from hematological cancers to rheumatoid arthritis. The synthesis of these drugs to selectively inhibit specific JAK-STAT has become an active area of research for pharmaceutical companies. Nitrogen heterocycles are commonly hydrogen deficient, presenting challenges for their characterization. The method of choice to determine the structure of these novel drugs is Heteronuclear Multiple Bond Correlation (HMBC) Nuclear Magnetic Resonance (NMR) spectroscopy. The limitation of this method is that it requires prior knowledge of the molecular orbital electron density of the molecule to be …

Temporal And Spatial Variation Of Nitrogen In Wyoming Big Sagebrush, Stephanie Williams, Jordan Nobler, Kristina Gehlken, Janet Rachlow, Lisa Shipley

College of Arts and Sciences Presentations

Sagebrush steppe systems represent one of North America’s greatest conservation challenges. Shrinking habitat and declining animal populations have prompted researchers to fill gaps in our understanding of the ecology of this system, particularly at a scale relevant to individual animals. What animals eat and why are fundamental questions linked to habitat quality and use. We investigated the temporal and spatial heterogeneity of the dietary quality of food to better understand habitat use by a sagebrush specialist, the pygmy rabbit (Brachylagus idahoensis). Within a foraging patch, pygmy rabbits selectively browse on individual Wyoming big sagebrush (Artemisia tridentata wyomingensis) that are …

Effects Of Zinc Oxide Nanoparticles On Carrot Roots And Arbuscular Mycorrhizae, Brian Husler

College of Arts and Sciences Presentations

Increased use of engineered nanoparticles (NPs) is likely to result in their continued release into the environment. Some nanoparticles including zinc oxide nanoparticles (ZnO-NPs) can be toxic to eukaryotic cells, but presently little is known about ZnO-NPs effects on plants and organisms symbiotically associated them. Under in vitro conditions, we examined the effect of 4 nm ZnO-NPs on carrots roots and arbuscular mycorrhizal fungi (AMF), which are obligate biotrophs that play an essential role in mineral uptake. During 4 weeks of incubation, roots in the control treatment (0μg ml^-1 ZnO) grew 30 ± 8 cm, while growth was negligible …

Barn Owl Sex Determination, Lillian Mckinley

College of Arts and Sciences Presentations

The goal of the study is to develop this model of sex determination in Barn Owls. The sex of birds of prey is usually determined using one of two methods: plumage or size. Plumage is often the most obvious way to tell the difference between male and female birds, because male birds often have bright and colorful plumage and female birds generally have drab and dull colored plumage. In species that do not have plumage variation, size difference is used for sex determination. The most common measurement of size is body mass, but other measurements can also be used including: …

Comparing And Informing Morphological Species Identifications And Boundaries In Arthropod Gut-Dwelling Protists Using Molecular Phylogenetic Analyses, Mason Hinchcliff, Nicole Reynolds, Eric Tretter, Dustin Heeney, Justin Gause, Tyler Pickell, Prasanna Kandel

College of Arts and Sciences Presentations

Trichomycetes are fungal and protistan symbionts of arthropods and have been found in marine, freshwater, and terrestrial habitats on every continent except Antarctica. Minimally, trichomycetes associate commensally with their immature aquatic hosts (including black flies, mayflies, stoneflies, isopods, and others) attaching to the chitinous lining of the mid or hindgut. Classified with trichomycetes are Paramoebidium, a group of protists which associate in an obligate manner with their hosts. To date, 16 species of Paramoebidium have been identified by morphological approaches. These descriptions have included characterization and discrimination of species based on thallus (body) length and width, differences in the …

Muscle Synergies During A Single-Leg Drop-Landing In Boys And Girls, Kristof Kipp, Ronald Pfeiffer, Michelle Sabick, Chad Harris, Jeanie Sutter, Seth Kuhlman, Kevin Shea

Kinesiology Faculty Publications and Presentations

The purpose of this study was to investigate muscle activation patterns during a landing task in boys and girls through the use of muscle synergies. Electromyographical (EMG) data from six lower extremity muscles were collected from 11 boys and 16 girls while they performed single-leg drop-landings. EMG data from six leg muscles were rectified, smoothed, and normalized to maximum dynamic muscle activity during landing. Data from 100 ms before to 100 ms after touchdown were submitted to factor analyses to extract muscle synergies along with the associated activation and weighing coefficients. Boys and girls both used three muscle synergies. The …