Life Sciences Commons^™

Ohio Wesleyan Bacillus Collection Student Research, Madeline Russell, Nandini Arora, Emily Bode, Morgan Chmielenski, Rebecca Coppenbarger, Ginny Faeth, Carianne Hutchison, Mindi Klaus, Jonna Mendenhall, Natalia Molotievskiy, Jess Nichols, Jennifer Paul, Brynn Schlesinger, Olivia Smith, Myles Steed, Abby Thierauf, Ivan Vore

Student Symposium

This semester students in BIOL 328 Bacterial Physiology lab worked in teams on a laboratory project that they devised, developed, and conducted. Each group met weekly with Dr. Tuhela-Reuning and Maddy Russell during the first third of the semester to develop a research project that involved the Bacillus culture collection at OWU. The Bacillus collection contains over 3000 isolates of Bacillus obtained from wild songbirds in Ohio, Arizona, and Washington, and many of these isolates degrade feathers. Student projects involved quantification of bird feather degradation by several Bacillus isolates, scanning electron microscopy (SEM) of biofilms and feather, possible correlation of …

Comparative Leaf Water Absorption Between Chaparral Island And Mainland Taxa: A Common Garden Experiment, Humera S. Mirza, Lauren M. Tucker, Rodrigo Méndez-Alonzo, Edward G. Bobich, Frank W. Ewers

Aliso: A Journal of Systematic and Floristic Botany

In California and other Mediterranean-type ecosystems, island species are typically exposed to more fog but less rain than mainland species. Because adaptations to absorb water from fog may conflict with those to minimize water loss, we hypothesized that island species should have greater fog absorption than their mainland congeners due to foliar uptake but at the cost of modifying other leaf structural and functional traits. To determine whether foliar water absorption is an adaptation to insularity, we compared seven physiological and anatomical leaf traits between congeneric island and mainland species of two genera, Ceanothus and Arctostaphylos, in a common …

Seed Morphology Of Some Plantago (Plantaginaceae) Species In Iran And Its Systematic And Phylogenetic Implications, Saeed Mohsenzadeh, Masoud Sheidai, Fahimeh Koohdar

Turkish Journal of Botany

Plantago is a cosmopolitan genus with approximately 200 species and various infrageneric classifications have been reported. In this study, the seed morphologies of 19 species of Plantago from Iran were studied and documented in detail using scanning electron microscopy and light microscopy. The studied species were grouped by using statistical analyses such as a minimum spherical cluster method based on Euclidean distance, principal coordinate analysis, and multidimentional scaling. We have described four types based on the seed shape and states of the inner side of the studied seeds: convex- angular type, convex-elliptic type, flat-elliptic type, and concave-elliptic type. In this …

Arterial Vascularization Of The Spleen In Merino Sheep, Melek Tosun, Şükrü Hakan Atalgin

Turkish Journal of Veterinary & Animal Sciences

In this study, it is aimed to reach detailed information about the anatomy of arteries in the sheep spleen, which can be used as an example and model for the success of planned or emergency surgical interventions related to the spleen. In spleen injuries, the spleen may be divided into two or three parts, or it may be limited to cracks on the spleen. In particular, it is aimed to protect the organs that have been damaged by trauma recently. Operations such as splenectomy and splenography are surgical procedures that require good knowledge of the pathways of artery and its …

Key To Isotomidae (Collembola) Of Iran And A Photographic Guide Of Key Characters Of Species, Elham Yoosefi Lafooraki, Jalil Hajizadeh, Masoumeh Shayanmehr, Reza Hosseini, Pietro Paolo Fanciulli

Turkish Journal of Zoology

Present work deals with providing an identification key to 60 Iranian species of the family Isotomidae. The photographic support provided here, both light micrographs and scanning electron microscopy (SEM) pictures, can be used as a practical tool for researchers who are not well acquainted with the morphology of Isotomidae.

“Revisiting The Past”: A Redescription Of Physaloptera Retusa (Nemata, Physalopteridae) From Material Deposited In Museums And New Material From Amazon Lizards = “Revisitando O Passado”: Uma Redescrição De Physaloptera Retusa (Nemata, Physalopteridae) A Partir De Material Depositado Em Museus E Novo Material De Lagartos Amazônicos, Lílian Cristina Macedo, Yuri Willkens, Leandro Maurício Oliveira Da Silva, Scott Lyell Gardner, Francisco Tiago De Vasconcelos Melo, Jeannie Nascimento Dos Santos

Scott L. Gardner Publications

Abstract

Physaloptera Rudolphi, 1819 is a genus of nematodes that includes approximately 100 species parasitic in vertebrates around the world. From these, approximately 30 occur in the Neotropical region, with nine reported from neotropical reptiles. Physaloptera spp. are recognized by their distinct morphology of the apical end and characters of the reproductive system. However, despite the fact that the morphological characters for species diagnosis have been firmly established, we frequently find identification problems regarding poorly detailed descriptions and poorly preserved specimens. These may lead to taxonomic incongruencies. Physaloptera retusa (Rudolphi, 1819) is the most common species of the genus and …

A Preliminary Investigation Into The Effects Of Lucilia Sericata (Meigen) Blow Fly Larval Feeding On Sharp Force Trauma (Sft) Wound Patterns In Decomposing Bones Of Sus Scrofa Domesticus, Erica L. Klafehn

Student Theses

An important aspect of forensic investigations involves the characterization and analysis of bone related trauma as this can provide valuable information regarding the manner and circumstances of death. Bone trauma can be classified into three main categories, which include Sharp Force Trauma (SFT), Blunt Force Trauma (BFT), and Gunshot Trauma. Previous experiments have explored SFT on flesh and bones, specifically made with knives or saws, coupled with various microscopic analytical methods. Minimal research has been done integrating both a forensic entomology and forensic anthropology approach, so it is imperative to understand previous literature detailing the behaviors of insects (blow flies), …

Achene Micromorphology Of The Genus Potentilla L. (Rosaceae) In Iran And Its Systematicapplication, Samira Sadeghi, Marzieh Beygom Faghir, Farideh Attar, Ali Alaami

Turkish Journal of Botany

Achene micromorphological evidence belonging to 32 species of Potentilla from Iran were studied using scanning electron microscopy (SEM). Multivariate analysis was used to estimate the potential contribution of the quantitative and qualitative traits to the species relationships. Based on the present results, the achene micromorphological traits exhibited considerable variations. The SEM observations revealed 4 types of achene shapes, 4 main types and 6 sub types of surface patterns, 3 types of wax, 8 types, 15 subtypes, and 4 categories of sculpturing, 2 types of anticlinal cell boundaries, and 3 types of outer periclinal walls. In addition, the results demonstrated variations …

Fibrinogen-Conjugated Gold-Coated Magnetite Nanoparticles For Targeting Activated Platelets In A Whole Blood System, Cammy Nu Truong

Theses and Dissertations

Activated platelets are a key component of the arterial thrombi responsible for heart attack and stroke. Tissue plasminogen activator (tPA) is currently the only FDA-approved drug for ischemic stroke. It works by dissolving fibrin, thus breaking down the clot and restoring blood flow to the brain. However, it must be administered within 4.5 hours of the onset of stroke. This leaves a significant number of patients who are ineligible for intervention and who have poor clinical outcomes. Additionally, tPA is not injury site-specific; consequently, the side effects range from mild to severe. We are investigating targeting activated platelets in occlusive …

Preparation Of Selenium Nanoparticles Based On Fish Gelatin Dispersion System And Investigation Of Their Anti-Oxidant Activities, Du Peng-Cheng, Wang Hui, Hu Yue-Ming, Tu Zong-Cai

Food and Machinery

Selenium nano particles (SeNPs) were prepared using fish gelatin dispersion system, and its particle size, morphology, and digestive stability were studied by dynamic light scattering (DLS) and scanning electron microscopy (SEM). The antioxidant capacity of SeNPs were determined. The results showed that fish gelatin was excellent dispersion system for preparation of SeNPs. The particle size of SeNPs and its morphology was barely affected by the concentration of fish gelatin dispersion system. Moreover, SeNPs presented good antioxidant ability. Among them, the DPPH radical scavenging ability of SeNPs prepared by 1.000%, 0.500%, 0.125% fish gelatin dispersion system, and IC50 value were 1.846 …

Characterization Of Extracellular Vesicles Isolated From Human Milk Using A Precipitation-Based Method, Diana C. Bickmore, John Miklavcic

Food Science Faculty Articles and Research

Extracellular vesicles (EV) function in intercellular communication, and those in human milk may confer immunologic benefits to infants. Methods of EV isolation such as ultracentrifugation (UC) may not be feasible for the study of EVs in human milk due to the need for large sample volume. A technique to isolate EVs from a small volume of human milk using a precipitation reagent is described herein. Electron microscopy, nanoparticle tracking analysis, and semi-quantitative antibody array were conducted to confirm isolation of human milk EVs. Count, size, protein content, and fatty acid quantification of EVs were determined. This isolation technique yielded 8.9 …

Light And Scanning Electron Microscopic Examination Of The Saanen Goat Heart, Sedef Selvi̇ler Si̇zer, Yonca Beti̇l Kabak, Murat Kabak

Turkish Journal of Veterinary & Animal Sciences

It is important to know the morphological structure of the heart, which is a vital organ of the body, in detail. For this reason, we aimed to examine the morphological structure of the Saanen goat heart using light and scanning electron microscopy (SEM). A total of thirteen Saanen goat hearts were used in the study. To demonstrate the histological structure of the heart, samples taken from 9 hearts were fixed in 10% formaldehyde solution and blocked in paraffin after routine histological procedures. Histological staining was performed on the sections taken from the blocks. Four hearts were fixed in a 2.5% …

Insecticidal Effect Of Diatomaceous Earth And Dolomite Powder Against Corn Weevilsitophilus Zeamais Motschulsky, 1855 (Coleoptera: Curculionidae), Alyssa Cristina Oliveira Freitas, Adriana Aparecida Sinopólis Gigliolli, Ronaldo Roberto Tait Caleffe Caleffe, Helio Conte

Turkish Journal of Zoology

Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae) which develops inside the grain, is an aggressive pest of stored grains. Here, we evaluated the efficiency of diatomaceous earth (DE), Diatomita CI/325 CX, and dolomite (DOL), Dolomita Dr. Kanyo, against S. zeamais reared on corn under laboratory conditions and observed external alterations on treated insects using scanning electron microscopy (SEM). Insects treated with both DE and DOL were impregnated with particles on the body surface, and they showed 100% mortality after 10 days of exposure. Damages caused by inert dusts may have contributed to dehydration and desiccation, consequently, leading insects to death. Thus, …

On The Use Of Vibrational Spectroscopy And Scanning Electron Microscopy To Study Phenolic Extractability Of Cooperage Byproducts In Wine, Berta Baca‑Bocanegra, Julio Nogales‑Bueno, Brian Gorey, Francisco José Heredia, Hugh Byrne, José Miguel Hernández‑Hierro

Articles

Wood is an important source of phenolic compounds, which can be transferred to wine during aging process, improving its properties, from an organoleptic point of view. Therefore, understanding and optimizing the extractability of phenolic compounds from wood are crucial in the oenological field. The structural composition of oak wood samples has been evaluated using Raman and attenuated total reflectance Fourier transform infrared (ATR–FTIR) spectroscopies, and their main spectral features have been linked to phenolic compound extractabilities, as measured by classic chemical analyses. To support the analysis, microscopic images of the samples were also recorded using scanning electron microscopy (SEM). The …

Fruit And Seed Macro- And Micromorphologies Of The Genus Matthiola (Brassicaceae) In Turkey And Their Taxonomic Value, Ayla Kaya, Murat Ünal, Abdurrahman Sefali, Esra Marti̇n

Turkish Journal of Botany

Macro- and micromorphological features of fruit and seeds belonging to 12 taxa genera of Matthiola W.T.Aiton were studied via stereomicroscope and scanning electron microscope. In macromorphological studies, the following variables were investigated: the shape, size, and color of fruit and seeds; the length of fruiting pedicel; the structure of the median vein; stigma (horns); and trichome properties and density in fruit. In micromorphological studies, the presence or absence of seed wings, seed coat pattern in disc and wing, and epidermal cell shape in disc and wing were determined. According to our findings, all characters of fruit and seeds are extremely …

Nutlet Structures Of Subsection Fragiles Of The Genus Stachys (Lamiaceae) From Turkey And Their Systematic Applications, Mehmet Cengi̇z Karai̇smai̇loğlu, Özal Güner

Turkish Journal of Botany

This paper includes the morphological and anatomical characters of the nutlets of 7 endemic species of the subsection Fragiles of the genus Stachys (Lamiaceae) from Turkey and the systematic significance of these characteristics, using one-way analysis of variance and cluster and principal component analyses. Morphological characters such as the dimensions, shapes, colors, and surfaces of the nutlets were studied using light and scanning electron microscopes. The structures and thicknesses of the pericarp and endosperm layers, as well as the sizes of the cotyledon in the nutlets, were anatomically studied. The outcomes revealed that the taxa varyfrom each other in terms …

Coagulation Factor Xiiia: Biochemical Properties Underlying Physiological Function., Boris Anokhin

Electronic Theses and Dissertations

Factor XIIIA (FXIIIA) is a transglutaminase that crosslinks intra- and extracellular protein substrates. The oligomeric state of active FXIIIA remains controversial, and the present work commenced with addressing this issue. The results of size exclusion chromatography and analytical ultracentrifugation confirmed a dimeric state for zymogen and, for the first time, indicated a monomeric state for the active FXIIIA in solution. Comparing sedimentation properties of proteolytically and nonproteolytically activated FXIIIA suggested conformational and functional differences between the two forms. Those differences were further assessed in a series of catalytic activity studies. Kinetic analysis revealed affinity for the glutamine substrate was higher …

Pollen Evidence Of Medicine From An Embalming Jar Associated With Vittoria Della Rovere, Florence, Italy, Karl Reinhard, Kelsey B. Lynch, Annie Larsen, Braymond Adams, Leon Higley, Marina Milanello Do Amaral, Julia Russ, Donatella Lippi, Johnica J. Morrow, Dario Piombino-Mascali

Karl Reinhard Publications

Various samples of human viscera fragments, sponges, and cloth were collected from embalming jars belonging to members of the Medici family of Florence. One jar was labeled with the name Vittoria della Rovere, who died in March of 1694. This jar contained viscera fragments that were identified as a section of collapsed intestine. The intestine of the Vittoria della Rovere sample contained a large concentration of pollen belonging to the Myrtaceae family. The Myrtaceae pollen was sometimes observed in clusters during analysis, which is indicative of purposeful ingestion of flowers, buds, or a substance derived from floral structures. Thus, the …

Microstructural Changes To Proso Millet Protein Bodies Upon Cooking And Digestion, Paridhi Gulati, You Zhou, Christian Elowsky, Devin J. Rose

Department of Food Science and Technology: Faculty Publications

Cooking results in a drastic decline in digestibility of proso millet proteins, panicins. Scanning electron and confocal microscopy were used to observe morphological changes in proso millet protein bodies upon cooking and digestion that could be associated with the loss in digestibility. Spherical protein bodies (1–2.5 mm) were observed in proso millet flour and extracted protein. Cooking did not result in any noticeable change in the size or shape of the protein bodies. However, upon digestion with pepsin the poor digestibility of cooked proso millet protein was clearly evident from the differences in microstructure of the protein bodies: large cavities …

Seed Structure And Its Taxonomic Implications For Genus Thlaspi Sensu Lato Sectionsnomisma, Thlaspi, And Pterotropis (Brassicaceae), Mehmet Cengi̇z Karai̇smai̇loğlu, Osman Erol

Turkish Journal of Botany

We studied morphological and anatomical characteristics of seeds of 22 taxa in the sections Nomisma, Thlaspi, and Pterotropis of Thlaspi sensu lato from Turkey and the significance of these characters in a taxonomical context. The results show that the taxa differ from each other in terms of seed shape and color. The seed size ranges from 1.25 mm to 2.99 mm in length and from 0.66 mm to 2.16 mm in width, Thlaspi rosulare and T. tatianae having the largest and T. annuum having the smallest seeds. The seed surface ornamentation is classified into nine types: reticulate-areolate, scalariform, reticulate, reticulate-foveate, …

Seed Structure Of Some Taxa Of The Genus Crocus L. (Iridaceae) Series Crocus, Mehmet Cengi̇z Karai̇smai̇loğlu, Levent Şik, Almila Gemi̇ci̇oğlu, Osman Erol

Turkish Journal of Botany

In this study, the macro- and micromorphological seed characteristics of the genus Crocus L. series Crocus taxa, including C. pallasii , C. turcicus , C. dispathaceus , C. asumaniae , C. mathewii , C. thomasii , C. oreocreticus , and C. cartwrightianus , were observed. Morphological characteristics, such as the size, shape, color, and seed surface structures, were analyzed using stereo and scanning electron microscopes. Testa layer structures including the outer and inner testae, phytomelan layer structure and thickness, and seed endosperm cell features were studied. Differences in the seed, raphe size, seed surface patterns, inner testa features, and phytomelan …

Biochemical And Histological Differences Between Costal And Articular Cartilages, Michael W. Stacey

Bioelectrics Publications

Biologically, costal cartilage is an understudied tissue type and much is yet to be learned regarding underlying mechanisms related to form and function, and how these relate to disease states, specifically chest wall deformity. Chest wall deformities have a component of inheritance, implying underlying genetic causes; however the complexity of inheritance suggests multiple genetic components. At our Centre investigations were performed on gene expression of key select genes from costal cartilage removed at surgery of patients with chest wall deformity to show high expression of decorin, a key player in collagen fiber formation and growth. Also, the degree of tissue …

Micromorphological Studies On The Genus Lotus L. (Fabaceae: Loteae) From Egypt, Momen Zareh, Ahmed Faried, Nashwa Farghaly

Turkish Journal of Botany

Trichomes, epidermal cell features, and seed coat sculpturing of 12 Lotus taxa from Egypt were studied using scanning electron microscopy. In addition, statistical analysis for the taxa under investigation was carried out. The results revealed that trichomes have high taxonomic significances between studied taxa. Anticlinal and periclinal cell walls and epidermal cell shape for the stem, leaf, and calyx for each taxon were examined. Seed coat sculpturing exhibited 4 main different surface patterns categories: rugose, reticulate, sulcate, and verrucate. These features can serve as good diagnostic characters at the species level of the genus Lotus.

Regulation Of Functional Activities And Essential Oil Production In Vetiveria Zizanioides L. Nash After γ-Irradiated Sodium Alginate Elicitation, Asfia Shabbir, Mohammad Masroor Akhtar Khan, Yawar Sadiq, Hassan Jaleel, Bilal Ahmad, Moin Uddin

Turkish Journal of Biology

Upon irradiation, marine polysaccharides undergo depolymerization, leading to formation of oligosaccharides that elicit various biological activities in plants. Taking a step further on the previously established growth-promoting activity of irradiated sodium alginate (ISA), structural rearrangements in ISA were analyzed using complementary techniques to develop an understanding of the structure-property relationship. The essential oil (EO) of vetiver (Vetiveria zizanioides L. Nash) immensely benefits the perfumery industry, proving itself as an economically important crop. A pot experiment was designed to test the effect of water-soluble ISA fractions on the growth, physiology, and EO production of vetiver. The structural characterization of radiation-induced sodium …

Needle Characteristics Of Lebanon Cedar (Cedrus Libani A.Rich.): Degradation Of Epicuticular Waxes And Decrease Of Photosynthetic Rates With Increasing Needle Age, Ayli̇n Güney, Reiner Zimmermann, Anna Krupp, Klaus Haas

Turkish Journal of Agriculture and Forestry

The evergreen conifer Cedrus libani A.Rich. İs frost- and drought-tolerant and of economic and ecological importance. It has valuable timber and thus has promising potential for cultivation within and outside its natural distribution. Our aim was to determine the morphological and physiological characteristics of C. Libani needle leaves with respect to epicuticular waxes, photosynthetic assimilation rates, and needle age. The study was conducted in the Cedar Research Forest near Elmalı, Antalya (SW Turkey) at 1650 m a.s.l. An equation to calculate needle surface area from needle length was created (R2 = 0.96) for adult cedar needles. The form factor for …

Vietnamese Ganoderma: Growth, Peculiarities, And Low-Molecular Composition Compared To European And Siberian Strains, Olga Tsivileva, Thao Nguyen, Long Vu, Nikolai Yurasov, Marina Chernyshova, Alexander Petrov, Viktor Galushka, Alexei Markin, Oleg Koftin

Turkish Journal of Botany

The species from the genus Ganoderma are known to be extremely important macrofungi in fundamental biological, medicinal, and socioeconomic aspects. The present communication describes the brief morphological description, macro- and microscopic details, and chemical constituents of the representatives of five species of Ganoderma mushroom collected from different sites in Vietnamese National Parks. These species were Ganoderma colossus, G. Neojaponicum, G. Cattienensis, G. Lucidum, and G. Applanatum. Three additional herbarium strains of Ganoderma from European and Siberian regions have been implemented in the present framework for the purposes of comparison. Cultural characterization on solid and liquid fermentation, and scanning electron microscopy …

Application Of 3d Printing Technology In Porous Anode Fabrication For Enhanced Power Output Of Microbial Fuel Cells, Bin Bian

Electronic Thesis and Dissertation Repository

Microbial fuel cells (MFCs) are widely researched for application in wastewater treatment. However, the current anodes used in MFCs often suffer from high fabrication cost and uncontrollable pore sizes. In this thesis, three-dimensional printing technique was utilized to fabricate anodes with different micro pore sizes for MFCs. Copper coating and carbonization were applied to the printed polymer anodes to increase the conductivity and specific surface area. Voltages of MFCs with various anodes were measured as well as other electrochemical tests such as linear sweep voltammetry and electrochemical impedance spectroscopy. 3D copper porous anode produced higher maximum voltages and power densities …

An Evaluation Of Biofield Treatment On Thermal, Physical And Structural Properties Of Cadmium Powder, Mahendra Kumar Trivedi, Gopal Nayak, Shrikant Patil, Rama Mohan Tallapragada, Omprakash Latiyal, Snehasis Jana

Mahendra Kumar Trivedi

Characterization Of Physical And Structural Properties Of Brass Powder After Biofield Treatment, Mahendra Kumar Trivedi, Gopal Nayak, Rama Mohan Tallapragada, Omprakash Latiyal, Snehasis Jana

Mahendra Kumar Trivedi

Impact Of Biofield Treatment On Physical, Structural And Spectral Properties Of Antimony Sulfide, Mahendra Kumar Trivedi, Gopal Nayak, Shrikant Patil, Rama Mohan Tallapragada, Omprakash Latiyal

Mahendra Kumar Trivedi