Life Sciences Commons^™

Providing Proteins To Belizean Residents Through Poultry Products, Ellis Freel

Poultry Science Undergraduate Honors Theses

Belize is a food insecure, developing country. Although Belize has plenty of nutritious food in-country, the exportation of that food to generate income contributed to a distribution issue of that nutritious food. More impoverished areas of Belize do not have access to nutritious food because of the lack of affordability and high export rates. Diets with a lack of food, or lack of balanced, nutritious food can negatively impact growth and cognitive development, especially in children. Poultry is one of the only products in Belize not able to be exported. Implementing poultry into Belizean diets would affordably alleviate food insecurity …

The Effects Of Two Levels Of Concentrates Supplying The Same Amount Of Protein On Silage Intake And Milk Production In Cows Given Two Grass Silages, H Khalili, P Huhtanen, K Rinne, M Suvitie

IGC Proceedings (1997-2023)

The objective of this trial was to investigate the effects of two levels of concentrate (5 and 10 kg) supplying the same amount of protein on feed intake, milk production and rumen fermentation in cows given two different grass silages. The silages were ensiled either with a formic acid based additive (F) or with a mixture of acids and lignosulphonates (L). Fermentation quality and digestibility were better in F silage than in L silage. There were no significant differences between the silages in feed intake, milk yield and milk composition. Feeding F silage increased rumen ammonia concentration and molar proportion …

Proteomic Characterization And Cytotoxic Potential Of Proteins From Cuscuta (Cuscuta Epithymum (L.) Crude Herbal Product Against Mcf-7 Human Breast Cancer Cell Line, Umaima Akhtar, Yamna Khurshid, Bishoy El-Aarag, Basir Syed, Ishtiaq A. Khan, Keykavous Parang, Aftab Ahmed

Pharmacy Faculty Articles and Research

Background

The burden of breast cancer, the second leading cause of death worldwide, is increasing at an alarming rate. Cuscuta, used in traditional medicine for different ailments, including cancer, is known for containing phytochemicals that exhibit anticancer activity; however, the bioactivities of proteins from this plant remain unexplored. This study aimed to screen the cytotoxic potential of proteins from the crude herbal product of Cuscuta epithymum(L.) (CE) harvested from the host plants Alhagi maurorum and Medicago sativa.

Methods

The proteins from CE were extracted using a salting-out method, followed by fractionation with a gel filtration chromatography column. Gel-free shotgun …

Identifying Key Evolving Residues That Drive Cid/Cal1 Incompatibility In Drosophila Species, Hayden Yuan, Barbara Mellone

Honors Scholar Theses

The centromere, an essential locus on the chromosome critically important for faithful chromosome segregation during cell division, requires the incorporation of centromere protein A (CENP-A/CID) orchestrated by the chaperone CAL1. This thesis investigates the structural requirements governing the interaction between CAL1 and CID, with a focus on two diverged Drosophila species. Using a combination of structural analysis prediction and cellular assays, I explored the compatibility between the N-terminal region of CAL1 and the L1 domain of CID. Previous results demonstrate species specific interactions between CAL1 and CID, with endogenous Drosophila melanogaster (mel) CAL1 efficiently depositing Drosophila melanogaster CID …

Winter Survival And Physiology Of Contrasting Fall Dormancy Selections Of Alfalfa, J J. Volenec, S M. Cunningham, L R. Teuber

IGC Proceedings (1997-2023)

Our objective was to determine the physiological changes that accompany selection from within a germplasm for contrasting fall dormancy reaction. Selection for greater fall dormancy improved winter survival of CUF 101 from 1 to 93%. The more fall dormant CUF 101 had higher sugar concentrations in buds and roots. Roots of the more fall dormant CUF 101 also contained higher soluble protein concentrations when compared to the other CUF 101 germplasms. Root protein extracts obtained in Dec. from the more fall dormant CUF 101 contained at least one polypeptide not found in protein extracts of the other CUF 101 germplasms. …

Root Nitrogen Cycling And Alfalfa Stress Tolerance, J J. Volenec, B C. Joern, L D. Barber, S M. Cunningham, A Ourry

IGC Proceedings (1997-2023)

Our hypothesis is that certain root N pools are utilized preferentially during the early shoot regrowth. Our objective was to determine the relative contribution of crown N, root N, and specific root N pools to shoot regrowth after defoliation. We used 15N to follow N into and out of crowns, roots, and specific root N pools, to regrowing shoots after defoliation. The low molecular weight N pool (amino acids, inorganic N,...) acquired 15N rapidly within 2 d of N application. Movement of 15N into the protein-N and insoluble-N pools was delayed initially, but continued until 8 d after N application. …

In-Silico Analysis For Structural And Functional Characterization Of Phosphorus-Starvation Tolerance 1 (Pstol1) Gene, Quratulain Mehdi Khan, Muhammad Aqeel, Maryam Murtaza, Wajya Ajmal, Muhammad Uzair, Sajid Fiaz, Kotb A. Attia, Asmaa M. Abushady, Itoh Kimiko, Muhammad Ramzan Khan, Ghulam Muhammad Ali

Turkish Journal of Agriculture and Forestry

As an important macro element for all living cells, phosphorus is essential in agricultural production systems as well and is required in large quantities by elite varieties of crops to maintain yields. Approximately 70% of worldwide cultivated land suffers from phosphorous deficiency, and it has recently been estimated that worldwide phosphorous resources will be shattered by the end of this century thus increasing the demand for crops to be more efficient in their P usage. A greater understanding of how plants can maintain yield with lower phosphorous availability is highly desirable to both breeders and farmers, therefore, significant to develop …

Effects On Intake Of Supplementing Low-Quality Roughage With Protein-Rich Feeds, J.J. M.H. Ketelaars, G A. Kaasschieter, M Kane

IGC Proceedings (1997-2023)

Intake responses of ruminants to supplementation with protein-rich concentrates or legume hays have been related to the ratio of nitrogen (N) content and organic matter digestibility (OMD) of the basal feed. Marginal intake effect of supplements, i.e. change of organic matter intake (OMI) from the basal feed per unit OMI from supplement, decreased on average from 1.7 to 0 and -0.8 g. g-1 at N/OMD of 0.010, 0.016 and > 0.030 g. g-1, respectively. Marginal effect of supplements defined as change of total digestible organic matter intake (DOMI) per g DOMI from supplement was 2.5, 1 and 0.3 g. g-1 for …

Forage Production Potential Of Poa Arachnifera Torr In Semi-Arid Climates, J C. Read, M A. Sanderson, G W. Evers, P W. Voigt, J A. Reinert

IGC Proceedings (1997-2023)

This study was undertaken to determine the forage potential of Texas bluegrass (Poa arachnifera Torr). Texas bluegrass is a perennial coolseason grass native to Kansas, Oklahoma, western Arkansas, and the central region of Texas, USA (Gould, 1975). Thirteen different accessions were established in 1988 at the Texas A&M University Centers at Dallas and Stephenville and tested for 2 years. Mean yields at Stephenville and Dallas were 3610 and 6022 kg ha-1. Two synthetic varieties (Syn-1 and Syn-2) were tested from 1990 to 1995 at four locations in Texas. Yields of Syn-1 was greater than Syn-2 whereas Syn- 2 provided …

Chlorophyll And Mineral Dynamics Of Perennial Grass Species Found In Semi- Arid Rangelands Of South Africa, N. H. Msiza, K. E. Ravhuhali, H. K. Mokoboki, Sydney Mavengahama, L. E. Motsei

IGC Proceedings (1997-2023)

Minerals are essential for ruminants and are mostly provided by grass species. Their growth stage is vital in the supply of these minerals. Perennial grass species have been mostly undervalued, owing to the scarce information concerning their potential feeding value and little documentation about their potential nutritive value in different growth stages. This study was conducted to investigate the chlorophyll and mineral concentration of grass species at different growth stages under greenhouse conditions at the North-West University Experimental farm, North West province, South Africa. The soil, collected from different villages, was mixed and samples were drawn for chemical analysis before …

Pathogenicity Of Acinetobacter Calcoaceticus, Kaitlan A. Sullivan

MUSC Theses and Dissertations

Acinetobacter is a genus of gram-negative bacteria that have been appearing frequently in hospitals contributing to infections in the blood, lungs, urinary tract, and other parts of the body. It infects patients with weakened immune systems that are placed on ventilators, after the use of catheters, or have any other open wounds produced by prolonged hospital stays. This genus of bacteria is problematic due to its high probability of becoming resistant to multiple classes of antibiotics. Thus, we are determining the pathogenicity of clinical isolates of Acinetobacter calcoaceticus using the organism Caenorhabditis elegans as a model.

We are testing …

Caulobacter Clpxp Adaptor Popa’S Domain Interactions In The Adaptor Hierarchy Of Ctra Degradation, Thomas P. Scudder

Masters Theses

The degradation and recycling of protein is a process essential for the maintenance and regulation of cellular function. More specifically, in Caulobacter crescentus, the ClpXP protease is responsible for driving progression through the cell cycle and protein quality control. This protease utilizes three known adaptors to selectively degrade proteins that initiate different stages of development. This thesis will elaborate on the specific binding interface on one of these adaptors, PopA, with another, RcdA, and focus in on specific residues on PopA and investigate their roles in adaptor binding and delivery of CtrA, the master regulator of Caulobacter. Finally, I …

Nutrition, Allergenicity And Physicochemical Qualities Of Food-Grade Protein Extracts From Nannochloropsis Oculata, Sara Hamzelou, Damien Belobrajdic, Angéla Juhász, Henri Brook, Utpal Bose, Michelle L. Colgrave, James A. Broadbent

Research outputs 2022 to 2026

Microalgae offer an opportunity to act as a sustainable source of dietary protein. This study aimed to evaluate the impact of different protein extraction methods on the nutritional and physicochemical properties of Nannochloropsis oculata. Food-grade protein extracts were obtained by hypotonic osmotic shock using milli-Q water. Food grade (FG) and non-food grade (NFG) extraction buffers were compared along with three cell disruption methods including bead beating, probe sonication and a combination of both methods for protein extraction. Mass spectrometry was used for protein and putative allergen identification in FG extracts. Bead beating led to a slightly higher number of identifiable …

Irrigation Increases On-Farm Soybean Yields In Water-Limited Environments Without A Trade-Off In Seed Protein Concentration, Walter D. Carciochi, Patricio Grassini, Seth Naeve, James E. Specht, Mitiku Mamo, Ron Seymour, Aaron Nygren, Nathan Mueller, Sarah Sivits, Christopher A. Proctor, Jenny Rees, Todd Whitney, N. Cafaro La Menza

Department of Agronomy and Horticulture: Faculty Publications

Context or problem: A trade-off between seed protein concentration (SPC) and yield has been reported for soybean. Therefore, assessing management practices that can nullify this trade-off is relevant to avoid further declines in SPC in the future as yield continues to increase. While the positive effect of irrigation on yield is well documented, only a few studies have assessed the impact of irrigation on SPC, showing conflicting results.

Objective or research question: The objective was to determine if the trade-off between seed yield and SPC persists when irrigation is applied and how management, soil, and weather factors influence the trade-off. …

International Society Of Sports Nutrition Position Stand: Effects Of Essential Amino Acid Supplementation On Exercise And Performance, Arny A. Ferrnado, Robert R. Wolfe, Katie R. Hirsch, David D. Church, Shiloah A. Kviatkovsky, Michael D. Roberts, Jeffrey R. Stout, Drew E. Gonzalez, Ryan J. Sowinski, Richard B. Kreider, Chad Kerksick, Nicholas A. Burd, Stefan M. Pasiakos, Michael J. Ormsbee, Shawn M. Arent, Paul J. Arciero, Bill I. Campbell, Trisha A. Van Dusseldorp, Ralf Jager, Darryn S. Willoughby, Douglas S. Kalman, Jose Antonio

Faculty Scholarship

Position Statement: The International Society of Sports Nutrition (ISSN) presents this position based on a critical examination of literature surrounding the effects of essential amino acid (EAA) supplementation on skeletal muscle maintenance and performance. This position stand is intended to provide a scientific foundation to athletes, dietitians, trainers, and other practitioners as to the benefits of supplemental EAA in both healthy and resistant (aging/clinical) populations. EAAs are crucial components of protein intake in humans, as the body cannot synthesize them. The daily recommended intake (DRI) for protein was established to prevent deficiencies due to inadequate EAA consumption. The following conclusions …

Kluyveromyces Marxianus Prepared As A Ready To Use Supplemental Food (Rusf), Zachary Christman

Applied Science Program: Theses

Ready to Use Supplemental Food (RUSF) is a nutrient dense paste or compressed bar used to supplement a person’s nutritional needs because of malnutrition or due to food shortages. The purpose of this article is to demonstrate some methods of how the dairy organism Kluyveromyces marxianus can be used to enrich the protein value of bread or ferment a substrate such as wheat bran into a more digestible form.

Protein Enriched Bread Using Spent Wine Yeast, Zachary Christman

Applied Science Program: Theses

Yeast is a common byproduct of wine production. Wine yeast may be used as a food source. It does not contain bitter and astringent compounds such as those present in spent yeast from making beer The use of nutritional yeast and yeast extract for protein enrichment of bread are presented in this article.

Saturating Growth Rate Against Phosphorus Concentration Explained By Macromolecular Allocation, Gabrielle Armin, Jongsun Kim, Keisuke Inomura

School of Earth, Environmental, and Marine Sciences Faculty Publications and Presentations

The saturating relationship between phytoplankton growth rate and environmental nutrient concentration has been widely observed, yet the mechanisms behind the relationship remain elusive. Here, we use a mechanistic model of phytoplankton and show that the saturating relationship between growth rate and phosphorous concentration can be interpreted by intracellular macromolecular allocation. At low nutrient levels, the diffusive nutrient transport linearly increases with the phosphorous concentration, while the internal phosphorous requirement increases with the growth rate, leading to a non-linear increase in the growth rate with phosphorous. This increased phosphorous requirement is due to the increased allocation to biosynthetic and photosynthetic molecules. …

An Appraisal Of The Potential For Soybeans In The United Kingdom, C. A. Sawyer, G. P. F. Lane, W. P. Davies

IGC Proceedings (1997-2023)

Soybean is a most important crop worldwide, accounting for 56% of world oilseed production and 69% of world protein meal consumption in 2003 (Soystats, 2004). Since their introduction in the early 1800's, forage soybeans have been grown widely in USA. Used originally as a forage crop, this use largely had been forgotten until Dr T. E. Devine (United States Department of Agriculture, Agricultural Research Service; USDA-ARS) released 4 new forage cultivars. Soybeans potentially offer UK farmers a high quality protein source in a short season and also meet the requirements of supermarket chains to remain GM free. This paper aims …

Towards Truly “Global” Near Infrared Calibrations For Protein And Neutral Detergent Fibre In Dried Ground Forages, D. Undersander, P. Berzaghi, P. Dardenne, P. Flinn, N. P. Martin, C. Paul, B. N. B. Büchmann, F. Mazeris, M. Lagerholm, I. A. Cowe

IGC Proceedings (1997-2023)

Over the past five years, Foss and DeLaval have sponsored the activities of a group of forage analysts with the aim of developing "global" Near Infrared (NIR) calibrations for parameters that are important in ruminant nutrition. The approach adopted has been based on the amalgamation of historical databases from centres worldwide and calibrations for protein and neutral detergent fibre (NDF) in dried ground forages have been developed based on databases that currently comprise approximately 30,000 records. Protein and NDF, while not the most important parameters in ruminant nutrition, were chosen for the initial calibration development exercise because of the amount …

Cry1 Expression During Postnatal Development Is Critical For The Establishment Of Normal Circadian Period, Aaron Schirmer, Vivek Kumar, Andrew Schook, Eun Joo Song, Michael S. Marshall, Joseph S. Takahashi

Biology Faculty Publications

The mammalian circadian system generates an approximate 24-h rhythm through a complex autoregulatory feedback loop. Four genes, Period1 (Per1), Period2 (Per2), Cryptochrome1 (Cry1), and Cryptochrome2 (Cry2), regulate the negative feedback within this loop. Although these proteins have distinct roles within the core circadian mechanism, their individual functions are poorly understood. Here, we used a tetracycline trans-activator system (tTA) to examine the role of transcriptional oscillations in Cry1 and Cry2 in the persistence of circadian activity rhythms. We demonstrate that rhythmic Cry1 expression is an important regulator of circadian period. We then define a critical period from birth to postnatal day …

Study On Antioxidant Properties Of Glycosylated Products Of Wheat Germ Protein, Niu Qiu-Yun

Food and Machinery

Objective: This study aimed to investigate the antioxidant properties of wheat germ protein glycosylation product. Methods: The glycation product (WGP-G) of wheat germ protein (WGP) and glucose was prepared by ultrasonic assisted moist heat method. Different ultrasonic time (10, 20, 30, 40, 50 and 60 min) and reaction temperature (70, 80 and 90 ℃) were measured. The browning degree, grafting degree, reducing power, hydroxyl radical scavenging capacity, superoxide anion radical scavenging capacity, DPPH radical scavenging capacity and ABTS+ radical scavenging capacity of WGP-G at 90 ℃ were evaluated comprehensively. Results: The browning degree of WGP-G increased with the increase of …

Research Progress On The Influence Of Protein On Physicochemical Properties, Microstructures And Digestive Properties Of Starch And Its Application, Zhao Hai-Bo, Sun Chun-Rui, Qiu Hong-Wei, Cui Bo, Cheng Yun-Hui, Chen Ling

Food and Machinery

Starch and protein are two important components in food system. The interactions between starch and protein have important influence on the structure, physicochemical properties of starch and the texture and digestibility of starch-based food. In recent years, the effect of exogenous protein on starch-protein interaction in mixed system and its application have become a hot research topic in the field of food. This article reviews the interaction mode between starch and protein, the effect of protein on physicochemical properties （gelatinization properties, retrogradation properties, rheological properties, thermal properties and texture properties） and microstructure of starch-protein mixed system, summarizes the regulation effect …

Biochemical Characterization Of Date Palm (Phoniex Dactylifera L) Seeds Maintained At Microgravity In Outer Space Iss, Shamma Khalid Aldhaheri

Theses

The UAE’s vision to reach space dates to 1976 when Sheikh Zayed Bin Sultan Al Nahyan met with the National Aeronautics and Space Administration (NASA) Apollo group; this meeting marked the first attempt of UAE to enter the field of space science. The UAE is currently one of the top countries executing space missions and research. This research focuses on the biochemical characterization of date palm seeds that are sent to the International Space Station (ISS) in outer space. The research methodology involves biochemical analysis of seeds maintained under two different environmental conditions-microgravity and standard gravity. In total, 30–45 seeds …

Effect Of Condensed Tannins In Sainfoin On In Vitro Protein Solubility Of Lucerne, J. Aufrère, M. Dudilieu, C. Poncet, R. Baumont

IGC Proceedings (1997-2023)

Proteins of fresh legume forages such as lucerne are highly degraded in the rumen, resulting in their inefficient use by the animal. The condensed tannins (CT) present in some forages can improve the nutritional value of these forages and of associated feeds in the diet. Previous in vitro work (Waghorn & Shelton, 1997) showed that CT from Lotus corniculatus are able to bind with and precipitate protein from a ryegrass/clover pasture, but when these forages were fed to sheep, the CT effects on digestion and animal performance were weak. This revealed a need for a better understanding of the mechanism …

Usage And Influence Of Dietary Supplementation Amongst Adolescent Athletes, Allison Harlow, Jennifer Walker, Aubrey Wagstaff

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

OBJECTIVE The purpose of this study was to investigate the use of dietary supplements or ergogenic aids amongst high school athletes.

Progress Towards Peptide And Protein Synthesis Involving Thiol Moieties, Ryan Lo Chen

Biological Sciences

Polypeptides and proteins play crucial roles in biological systems, including enzymatic activity regulation, molecule transport, and cell and tissue structural support. Amino acids are the building blocks of peptides and proteins, and peptides can act as hormones, neurotransmitters, and enzymes. Proteins are made up of one or more polypeptide chains and have diverse functions such as catalyzing chemical reactions, transporting molecules, and providing structural support. Protein preparation can be done biologically by expression systems or isolation and purification from natural sources. While these methods have their advantages, they also have their shortcomings. Chemical synthesis is an alternative method that allows …

Optimizing Soil Nutrient Management To Improve Dry Edible Bean Yield And Protein Quality, Emily Jundt

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Soil nutrient management is of the utmost importance to ensure crop yields are maximized to feed the world’s growing population. Micro and macronutrient deficiencies can be detrimental to plant growth and cause a drastic reduction in yield. Optimization of nutrient management needs extensive research. Nutrients such as nitrogen increase crop yield, even in legumes capable of using a large amount of nitrogen (N) via biological N fixation. Iron (Fe) fertilizers are also crucial for legumes as they are susceptible to Fe deficiency induced chlorosis.

Dry edible beans (Phaseolus vulgaris) are leguminous plants and excellent dietary protein sources. Great …

Investigating The Helicase Activity Of Methylated Vs Unmethylated Ded1, Hannah Lukow

Honors Theses

Ded1 is an RNA helicase protein of the DEAD-box subfamily in eukaryotic organisms (Sharma & Jankowsky, 2014) which can act as an activator or initiation factor, during translation (Hilliker et al., 2011). Ded1 has several functions in yeast including assembly of translational initiation factors, scanning the mRNA for the start codon, and unwinding any double stranded segments of mRNA with its helicase ability. Ded1 was discovered to be methylated at four arginine sites in vivo (Low et al., 2013), with a fifth methylation site being discovered recently (Low et al., 2020), however the purpose of such post-translational modifications is still …

Study On Process Optimization And Antioxidant Properties Of Passion Fruit And Apple Compound Fermented Milk Beverage, Jia Qing-Chao

Food and Machinery

Objective: Using passion fruit, apple and pure milk as raw materials, the optimum preparation technology and antioxidant activity of passion fruit apple flavor fermented milk were studied. Methods: Taking sensory evaluation and protein content as indicators, the effects of apple juice addition, passion fruit juice addition, sucrose addition, fermentation time and starter inoculation on sensory quality of passion fruit apple flavor fermented milk were studied by single factor test using fuzzy mathematics evaluation method. The fermentation process conditions of passion fruit apple flavor fermented milk were optimized by response surface methodology, and the antioxidant activity and various indexes of fermented …