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Non-Antibiotic Selection Systems For Soybean Somatic Embryos: The Lysine Analog Aminoethyl-Cysteine As A Selection Agent, Suryadevara S. Rao, Lewamy Mamadou, Matt Mcconnell, Raghuveer Polisetty, Prachuab Kwanyuen, David Hildebrand
Non-Antibiotic Selection Systems For Soybean Somatic Embryos: The Lysine Analog Aminoethyl-Cysteine As A Selection Agent, Suryadevara S. Rao, Lewamy Mamadou, Matt Mcconnell, Raghuveer Polisetty, Prachuab Kwanyuen, David Hildebrand
Plant and Soil Sciences Faculty Publications
BACKGROUND: In soybean somatic embryo transformation, the standard selection agent currently used is hygromycin. It may be preferable to avoid use of antibiotic resistance genes in foods. The objective of these experiments was to develop a selection system for producing transgenic soybean somatic embryos without the use of antibiotics such as hygromycin.
RESULTS: When tested against different alternate selection agents our studies show that 0.16 microg/mL glufosinate, 40 mg/L isopropylamine-glyphosate, 0.5 mg/mL (S-(2 aminoethyl)-L-cysteine) (AEC) and the acetolactate synthase (ALS) inhibitors Exceed and Synchrony both at 150 microg/mL inhibited soybean somatic embryo growth. Even at the concentration of 2 mg/mL, …