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Full-Text Articles in Life Sciences
Common-Path Multimodal Optical Microscopy, Marla Tipping, Chandra S. Yelleswarapu, Sri-Rajasekhar Kothapalli, Alexey Veraksa, D.V.G.L.N. Rao
Common-Path Multimodal Optical Microscopy, Marla Tipping, Chandra S. Yelleswarapu, Sri-Rajasekhar Kothapalli, Alexey Veraksa, D.V.G.L.N. Rao
Biology Faculty Publications
We have developed a common-path multimodal optical microscopy system that is capable of using a single optical source and a single camera to image amplitude, phase, and fluorescence features of a biological specimen. This is achieved by varying either contrast enhancement filters at the Fourier plane and/or neutral density/fluorescence filters in front of the CCD camera. The feasibility of the technique is demonstrated by obtaining brightfield, fluorescence, phase-contrast, spatially filtered, brightfield + fluorescence, phase +fluorescence, and edge-enhanced+fluorescence images of the same Drosophila embryo without the need for image registration and fusion. This comprehensive microscope has the capability of providing both …
Localization Of Ufd2a Transcripts In Adult And Fry Zebrafish, Serena J. Kankash
Localization Of Ufd2a Transcripts In Adult And Fry Zebrafish, Serena J. Kankash
Honors Projects
Examines the spacio-temporal expression of the three alternative splice forms of UFD2A in zebrafish to further understanding of UFD2A's role in muscle development
Expression Of Three Novel Alternative Splice Forms Of Ufd2a During Zebrafish Development, Nicole Gadbois
Expression Of Three Novel Alternative Splice Forms Of Ufd2a During Zebrafish Development, Nicole Gadbois
Honors Projects
Ufd2a is a ubiquitylation enzyme that is involved in protein degradation, cell division, and apoptosis signaling. Data has shown that cardiac and skeletal muscle cells of adult rodents and humans express alternatively spliced isoforms of Ufd2a. Ufd2a I (exons 1-6, 8-27) has been found to be expressed in murine myoblasts, but, upon differentiation, myotubes began to express Ufd2a II (includes exon 7), followed by Ufd2a III (includes exon 7a). Since zebrafish are an ideal in vivo model organism, this study examines the spatial-temporal expression patterning of Ufd2a and its isoforms in zebrafish embyros during development.