Life Sciences Commons^™

Sagestep News, Winter 2009, No. 8, Sagestep

Newsletters

Seasonal newsletter of SageSTEP.

Sagestep News, Summer/Fall 2009, No. 10, Sagestep

Newsletters

Seasonal newsletter of SageSTEP.

Sagestep News, Spring 2009, No. 9, Sagestep

Newsletters

Seasonal newsletter of SageSTEP.

Rapid Screening For Temperature-Sensitive Alleles In Plants, L Vidali, Rc Augustine, Sn Fay, P Franco, Ka Pattavina, M Bezanilla

Magdalena Bezanilla

We developed a simple and fast method to identify temperature-sensitive alleles of essential plant genes. We used primary and tertiary structure information to identify residues in the core of the protein of interest. These residues were mutated and tested for temperature sensitivity, taking advantage of the exceptionally rapid 1-week complementation assay in the moss Physcomitrella patens. As test molecules, we selected the actin-binding proteins profilin and actin-depolymerizing factor, because they are essential and their loss-of-function phenotype can be fully rescued. Screening a small number of candidate mutants, we successfully identified temperature-sensitive alleles of both profilin and actin-depolymerizing factor. Plants harboring …

Lifeact-Megfp Reveals A Dynamic Apical F-Actin Network In Tip Growing Plant Cells, L Vidali, Cm Rounds, Pk Hepler, M Bezanilla

Magdalena Bezanilla

Background

Actin is essential for tip growth in plants. However, imaging actin in live plant cells has heretofore presented challenges. In previous studies, fluorescent probes derived from actin-binding proteins often alter growth, cause actin bundling and fail to resolve actin microfilaments.

Methodology/Principal Findings

In this report we use Lifeact-mEGFP, an actin probe that does not affect the dynamics of actin, to visualize actin in the moss Physcomitrella patens and pollen tubes from Lilium formosanum and Nicotiana tobaccum. Lifeact-mEGFP robustly labels actin microfilaments, particularly in the apex, in both moss protonemata and pollen tubes. Lifeact-mEGFP also labels filamentous actin structures in …

Blue Mountain Site Quick Facts, Sagestep

Fact Sheets

This site is part of the study of woodland encroachment into sagebrush communities. The primary objective is to find out how much native sagebrush and bunchgrasses need to be present at the time of treatment in order for managers to improve land health without having to conduct expensive re-seeding. The Doublehead Ranger District implemented treatments in the fall of 2007.

Greenville Bench Site Quick Facts, Sagestep

Fact Sheets

This site is part of the study of woodland encroachment into sagebrush communities. The primary objective is to find out how much native sagebrush and bunchgrasses need to be present at the time of treatment in order for managers to improve land health without having to conduct expensive re-seeding. Treatments were implemented at this site in fall 2007.

Owyhee Site Quick Facts, Sagestep

Fact Sheets

This site is part of the study of cheatgrass invasion of sagebrush communities. The primary objective is to find out how much native bunchgrasses need to be present at the time of treatment in order for managers to improve land health without having to conduct expensive re-seeding. Treatments were implemented at this site in fall 2008.

Marking Corral Site Quick Facts, Sagestep

Fact Sheets

This site is part of the study of woodland encroachment into sagebrush communities. The primary objective is to find out how much native sagebrush and bunchgrasses need to be present at the time of treatment in order for managers to improve land health without having to conduct expensive reseeding. Treatments were implemented at this site in fall 2006.

Saddle Mountain Quick Facts, Sagestep

Fact Sheets

This site is part of the study of cheatgrass invasion of sagebrush communities. The primary objective is to find out how much native bunchgrasses need to be present at the time of treatment in order for managers to improve land health without having to conduct expensive re-seeding. Treatments were implemented at this site in fall 2008.

Castlehead Site Quick Facts, Sagestep

Fact Sheets

This site was partially burned by a wildfire in the summer of 2007. A prescribed burn on the remainder of the plot was attempted in fall 2007 but was unsuccessful due to weather. As a result, post-treatment data collection will be limited to the wildlife and hydrology studies.

Spruce Mountain Site Quick Facts, Sagestep

Fact Sheets

This site is part of the study of woodland encroachment into sagebrush communities. The primary objective is to find out how much native sagebrush and bunchgrasses need to be present at the time of treatment in order for managers to improve land health without having to conduct expensive reseeding. Treatments are planned for 2009.

Stansbury Big Pole Site Quick Facts, Sagestep

Fact Sheets

This site is part of the study of woodland encroachment into sagebrush communities. The primary objective is to find out how much native sagebrush and bunchgrasses need to be present at the time of treatment in order for managers to improve land health without having to conduct expensive re-seeding. Treatments were implemented at this site in fall 2007.

Bridge Creek Site Quick Facts, Sagestep

Fact Sheets

This site is part of the study of woodland encroachment into sagebrush communities. The primary objective is to find out how much native sagebrush and bunchgrasses need to be present at the time of treatment in order for managers to improve land health without having to conduct expensive re-seeding. The BLM Prineville District Office implemented treatments in fall 2006.

Devine Ridge Site Quick Facts, Sagestep

Fact Sheets

This site is part of the study of woodland encroachment into sagebrush communities. The primary objective is to find out how much native sagebrush and bunchgrasses need to be present at the time of treatment in order for managers to improve land health without having to conduct expensive re-seeding. The BLM Burns District implemented treatments at in the fall of 2007.

Five Creeks Site Quick Facts, Sagestep

Fact Sheets

This site is part of the study of woodland encroachment into sagebrush communities. The BLM Burns District Office implemented treatments in fall 2008.

Hart Mountain Gray Butte And Rock Creek Sites Quick Facts, Sagestep

Fact Sheets

These sites are part of the study of cheatgrass invasion of sagebrush communities. The primary objective is to find out how much native bunchgrasses need to be present at the time of treatment in order for managers to improve land health without having to conduct expensive reseeding. Treatments were implemented at Rock Creek in 2007 and Gray Butte in 2008.

Moses Coulee Site Quick Facts, Sagestep

Fact Sheets

This site is part of the study of cheatgrass invasion of sagebrush communities. The primary objective is to find out how much native bunchgrasses need to be present at the time of treatment in order for managers to improve land health without having to conduct expensive re-seeding. Treatments were implemented at this site in fall 2008, except for the burn, which is planned for 2009.

Onaqui Research Sites, Sagestep

Fact Sheets

Two of the SageSTEP study sites are located in Rush Valley, Utah, just 90 minutes from Salt Lake City. The nearest towns are Vernon (~5 miles) and Tooele (~25 miles ), and the land is managed by the Bureau of Land Management (BLM) Salt Lake Field Office. Onaqui is the only SageSTEP network location with two sites of different vegetation composition adjacent to each other: a sagebrush/cheatgrass site, and a sagebrush/Utah juniper site.

Roberts Site Quick Facts, Sagestep

Fact Sheets

This site is part of the study of cheatgrass invasion of sagebrush communities. The primary objective is to find out how much native bunchgrasses need to be present at the time of treatment in order for managers to improve land health without having to conduct expensive re-seeding. Treatments were implemented at this site in fall 2007.

Scipio Site Quick Facts, Sagestep

Fact Sheets

This site is part of the study of woodland encroachment into sagebrush communities. The primary objective is to find out how much native sagebrush and bunchgrasses need to be present at the time of treatment in order for managers to improve land health without having to conduct expensive re-seeding. Treatments were implemented at this site in fall 2007.

Seven Mile Site Quick Facts, Sagestep

Fact Sheets

This site is part of the study of woodland encroachment into sagebrush communities. The primary objective is to find out how much native sagebrush and bunchgrasses need to be present at the time of treatment in order for managers to improve land health without having to conduct expensive reseeding. Treatments were implemented at this site in fall 2007.

South Ruby Mountain Quick Facts, Sagestep

Fact Sheets

This site is part of the study of woodland encroachment into sagebrush communities. The primary objective is to find out how much native sagebrush and bunchgrasses need to be present at the time of treatment in order for managers to improve land health without having to conduct expensive reseeding. Treatments were implemented at this site in fall 2008.

Stansbury Site Quick Facts, Sagestep

Fact Sheets

This site is part of the study of woodland encroachment into sagebrush communities. The primary objective is to find out how much native sagebrush and bunchgrasses need to be present at the time of treatment in order for managers to improve land health without having to conduct expensive re-seeding. Treatments were implemented at this site in fall 2007.

Walker Butte Site Quick Facts, Sagestep

Fact Sheets

This site is part of the study of woodland encroachment into sagebrush communities. The primary objective is to find out how much native sagebrush and bunchgrasses need to be present at the time of treatment in order for managers to improve land health without having to conduct expensive re-seeding. The BLM Lakeview Resource Area implemented treatments in fall 2006.

Southwest Research-Extension Center, Field Day 2009

Kansas Agricultural Experiment Station Research Reports

Report of agricultural research from Southwest Research-Extension Center of Kansas State University.