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Full-Text Articles in Life Sciences
Uv Resonance Raman And Circular Dichroism Studies Of A Dna Duplex Containing An A3t3 Tract: Evidence For A Premelting Transition And Three-Centered H-Bonds, Ishita Mukerji
Ishita Mukerji
The presence of A(n) and A(n)T(n) tracts in double-helical sequences perturbs the structural properties of DNA molecules, resulting in the formation of an alternate conformation to standard B-DNA known as B'-DNA. Evidence for a transition occurring prior to duplex melting in molecules containing A(n) tracts was previously detected by circular dichroism (CD) and calorimetric studies. This premelting transition was attributed to a conformational change from B'- to B-DNA. Structural features of A(n) and A(n)T(n) tracts revealed by X-ray crystallography include a large degree of propeller twisting of adenine bases, narrowed minor grooves, and the formation of three-centered H-bonds between dA …
Spectroscopic And Molecular Dynamics Evidence For A Sequential Mechanisms For The A-To-B Transition In Dna, Ishita Mukerji
Spectroscopic And Molecular Dynamics Evidence For A Sequential Mechanisms For The A-To-B Transition In Dna, Ishita Mukerji
Ishita Mukerji
The A-to-B form transition has been examined in three DNA duplexes, d(CGCGAATTCGCG)2, d(CGCGAATTGCGC), and d(CGCAAATTTCGC), using circular dichroism spectroscopy, ultraviolet resonance Raman (UVRR) spectroscopy, and molecular dynamics (MD) simulation. Circular dichroism spectra confirm that these molecules adopt the A form under conditions of reduced water activity. UVRR results, obtained under similar conditions, suggest that the transition involves a series of intermediate forms between A and B. Cooperative and distinct transitions were observed for the bases and the sugars. Independent MD simulations on d(CGCGAATTCGCG)2 show a spontaneous change from the A to B form in aqueous solution and describe a kinetic …
Integration Host Factor (Ihf) Dictates The Structure Of Polyamine-Dna Condensates: Implications For The Role Of Ihf In The Compaction Of Bacterial Chromatin, Ishita Mukerji
Ishita Mukerji
No abstract provided.
Effects Of Hu Binding On The Equilibrium Cyclization Of Mismatched, Curved And Normal Dna, Ishita Mukerji
Effects Of Hu Binding On The Equilibrium Cyclization Of Mismatched, Curved And Normal Dna, Ishita Mukerji
Ishita Mukerji
No abstract provided.