Life Sciences Commons^™

Photo Highlights Of The 10th Kentucky Grazing Conference, Kentucky Grazing Conference

Kentucky Grazing Conference

No abstract provided.

Forage Spokesman Information, Tom Flowers, Vince Rawe, Caldwell Willig

Kentucky Grazing Conference

No abstract provided.

Animal Welfare--Animal Rights: Role Of Grazing, Roy Burris

Kentucky Grazing Conference

The care and handling of farm animals has drawn a lot of media attention lately. Some well-publicized events have given our industry the proverbial black-eye and led to a chain of events that has left livestock producers feeling very vulnerable to negative attacks. Although these events have been very isolated, they have had some very farreaching effects. Could they have been prevented? How? What do we do now?

Extended Grazing And Reduced Stored Feed, Edward N. Ballard

Kentucky Grazing Conference

Feed costs represent the major cost in most livestock production systems. A recently completed analysis of 225 Standardized Performance Analysis (SPA) Beef Cow Records on herds in Illinois and Iowa showed that feed cost was the overriding factor determining profitability, explaining over 57 percent of the herd-to-herd variation. Typically the cost of supplying nutrients to ruminant livestock is much greater using harvested feedstuffs as opposed to grazing pastures or crop residues. The primary function of a grassland farm is to convert solar energy to marketable livestock products in the most efficient manner. The fewer steps between the animal product and …

Stored Feed Needs: How Much? What Quality?, Jeff Lehmkuhler

Kentucky Grazing Conference

Feed resources are the largest variable expense in beef production systems outside the initial investment in the livestock. When we look at Standardized Performance Analysis (SPA) information from Texas and Minnesota, states that are at the extreme from an environment stance, the projected costs for maintaining a beef cow are strikingly similar. It is important to note that these costs include all expenses and opportunity costs. Getting back to the SPA information, in 2007 the annual cost to maintain a cow in Texas was determined to be roughly $590 while the Minnesota value was near $610 or only about $20/cow …

Tall Fescue Grazing Research, Glen Aiken

Kentucky Grazing Conference

‘Kentucky-31’ tall fescue, like it or hate it, covers 5 million acres of Kentucky and if you do not have it on your farm, it is likely that one of your bordering neighbors does! Tall fescue owes its persistence and productivity in large part to a fungal endophyte that infects most plants. This endophyte produces an array of alkaloids that benefit the plant in tolerating heat, drought, and grazing. Unfortunately, the endophyte also produces ergot alkaloids that can cause “fescue toxicosis” in cattle. Cattle inflicted with toxicosis have reduced dry matter intake, elevated body temperature, and often retain their winter …

Weed Control Options For Pastures, Jon Doran

Kentucky Grazing Conference

What is a weed? A pasture or hayfield “weed” is any plant that you deem as being out of place and/or a plant that is decreasing your desired forage production. Weeds may be classified in several ways including broadleaf weeds, woody plants, and undesirable grasses. Besides decreasing forage, weeds can also interfere with forage utilization, grazing distribution, etc. There are several methods available to control weeds and increase production of and access to desirable forages. The three main methods are cultural, mechanical, and chemical. The cultural method is quite simple-a thriving grass stand is an excellent form of weed control …

Our Experiences With Eastern Gamagrass, Tommy R. Yankey

Kentucky Grazing Conference

As livestock producers try to reduce their cost of production, many look at ways to reduce their feed costs. Feed costs have been identified as the largest single cost of livestock production, making up 50 to 70% of the total cost of production. Grazing is the cheapest way to feed cattle on a cost per pound of nutrient basis.

New Developments In Grazing Species And Varieties, S. Ray Smith

Kentucky Grazing Conference

During the last 10 years there have been several new forage species that have emerged as useful in grazing systems and many new varieties have been released of traditional species. Plant breeders continue to make improvement in our traditional pasture species like tall fescue, orchardgrass, and red and white clover. In the following paragraphs, I will focus more on some of the forage species that you may not be as familiar with and highlight the advancements occurring in developing new varieties for grazing. There are many sources of information on this subject. I especially encourage you to read the new …

Why Should I Improve My Grazing Program?, Garry D. Lacefield

Kentucky Grazing Conference

Grazing represents the cheapest way to feed ruminants on a cost per pound of nutrient basis. Stored feed is usually the single largest item in livestock budgets and cost or amount of stored feed is usually the best prediction of potential profitability in most beef cattle operations.

Foreword And Kfgc Award Winners [2009], Garry D. Lacefield, Christi L. Forsythe

Kentucky Grazing Conference

No abstract provided.

Artificial Intelligence – Ii: Network Path Optimization Using Ga Approach, Madiha Sarfraz, Shaleeza Sohail, Younus Javed, Almas Anjum

International Conference on Information and Communication Technologies

In this paper, we present a variation of Genetic Algorithm (GA) for finding the Optimized shortest path of the network. The algorithm finds the optimal path based on the bandwidth and utilization of the network. The main distinguishing element of this work is the use of ldquo2-point over 1-point crossoverrdquo. The population comprises of all chromosomes (feasible and infeasible). Moreover, it is of variable length, so that the algorithm can perform efficiently in all scenarios. Rankbased selection is used for cross-over operation. Therefore, the best chromosomes crossover and give the most suitable offsprings. If the resulting offsprings are least fitted, …

Artificial Intelligence – Ii: Retinal Image Blood Vessel Segmentation, M. Usman Akram, Anam Tariq, Shoab A. Khan

International Conference on Information and Communication Technologies

The appearance and structure of blood vessels in retinal images play an important role in diagnosis of eye diseases. This paper proposes a method for segmentation of blood vessels in color retinal images. We present a method that uses 2-D Gabor wavelet to enhance the vascular pattern. We locate and segment the blood vessels using adaptive thresholding. The technique is tested on publicly available DRIVE database of manually labeled images which has been established to facilitate comparative studies on segmentation of blood vessels in retinal images. The proposed method achieves an area under the receiver operating characteristic curve of 0.963 …

Artificial Intelligence – I: Dna Innovate: Visualizing Dna Sequences, M. Shahzad, Nazish Alia, Sadaf Mahmood

International Conference on Information and Communication Technologies

Exploring DNA sequences is really important in the context of bioinformatics, as it maintains every function of our body - being the building block. Bioinformaticians have unfolded numerous features of this sequence and it is probable that still there are more features to discover within DNA sequence. To get the desired information from DNA sequence quickly, depends largely on its presentation. It is desirable to investigate the sequence in visual or textual form, as needed. In this paper, we put forward a software tool which can be helpful in exploring DNA features and observing it in different representations (simultaneously or …

Stationary Phase Mutagenesis In Bacillus Subtilis: The Interaction Between Transcription And Error-Prone Replication In Conditions Of Stress, Mary Girard, Eduardo Robleto

Undergraduate Research Opportunities Program (UROP)

While under conditions of stress, non-dividing cells may acquire beneficial mutations. This is referred to as stationary phase mutagenesis, or adaptive mutagenesis. Previous research has shown that actively transcribed genes and those under selective pressure are prone to mutations that confer escape from non-dividing conditions. Accordingly, strains lacking transcription factors have shown a drastically lower number of mutations that confer escape while under amino acid starvation than those observed in the wildtype background. Also, error-prone DNA polymerases are known to be active in cells under stress and it has been shown that strains lacking an error-prone DNA polymerase display reduced …

Long Distance Microbial Transport In Air: Global Change Implications, Bradley J. Davey, J. C. Bruckner, Jenny C. Fisher, Duane P. Moser

Undergraduate Research Opportunities Program (UROP)

The first manifestations of global change will most likelv be observed in the Earth's atmosphere. Changing wind patterns, for example, may effect the long distance dispersal of microor-g anisms. The overall objective of this research is to correlate molecular assessments of microbial community structure from cloud water and snow samples, obtained from DRI's Storm Peak Laboratory atop Mt. Werner in Colorado, with atmospheric data and calculated air mass back trajectories. Our activities for summer of 2009 will be a focused proof-of-concept exercise to determine if intact microbial DNA and viable cells can be recovered from cloud water and alpine snow …

Pdest Fg12-Cmv Dsred Vector, Jarod Wolffis, Sheri L. Holmen

Undergraduate Research Opportunities Program (UROP)

Melanoma is the most rapidly increasing malignancy among young people in the United States. If detected early, the disease is easily treated; however, once the disease has metastasized it is largely refractory to conventional therapies and is associated with a high mortality rate. The development of human cancer from a pre-malignant primary tumor to a metastatic lesion that develops at secondary sites is thought to be a multi-step process, requiring many genetic and epigenetic events that provide a growth advantage to cells. It is still unclear which of the many genetic changes in human cancers are required for metastasis. Therefore, …

Attempts To Cultivate Bacteria From Deep Subsurface Aquifers And Mountaintop Plant Communities, Eric D. Hughes, J. C. Bruckner, Duane P. Moser

Undergraduate Research Opportunities Program (UROP)

In the late 1990s, the limits of life were pushed even further when microorganisms were discovered thriving 2.5 km below the surface of the Earth in deep South African gold mines. These very simple communities were dominated by a single species of bacteria from within the phylum, Firmicutes. Desulforudis audaxviator remains unique to a sizeable portion of the South African deep subsurface. At depths below 2.5km, it comprises well over 99% of all organisms present, which presents a unique circumstance in which the environment has provided a natural pure culture. From this naturally occurring pure culture, environmental genomics was applied …

The Role Of Rpoe In Stationary Phase Mutagenesis In Bacillus, Turquoise C. Alexander, Eduardo A. Robleto

Undergraduate Research Opportunities Program (UROP)

Stationary phase mutagenesis is a phenomenon whereby random mutations are generated in non-dividing cells. In order to understand how these mutations arise, we use Bacillus subtilis, a gram positive rod-shaped model organism. It is hypothesize that increased transcription promotes stationary phase mutagenesis in this organism. We therefore examined the role of rpoE, a gene that encodes RNA polymerase ! subunit and proposed to influence efficiency of transcription. To this end, we will first generate a strain bearing a deletion in the rpoE gene. In order to determine if this gene is important for mutagenesis, we will examine the accumulation of …

Point And Nonpoint Source Analysis Of Nutrients, Metals, And Pathogens In The Sediment And Water Column In Las Vegas Wash, Christine Simmons, Kumud Acharya

Undergraduate Research Opportunities Program (UROP)

Formerly an ephemeral watercourse, Las Vegas Wash is now a perennial system due to urban runoff and wastewater treatment plant (WWP) effluent. Las Vegas Wash flows into Lake Mead, where the discharge point is only a few miles upstream of Las Vegas’ main water intake. This small water cycle establishes the necessity to evaluate water quality especially due to non point sources pollution, wherein my research lies. Several points along Las Vegas Wash upstream and downstream of WWP have been chosen to represent different landuse types such as commercial, residential, wastewater treatment plants, etc. At each location, parameters including arsenic, …

The Role Of Recn In Stationary Phase Mutagenesis In Bacillus Subtilis, Lauren E. Johnson, Katherine R. Ona, Eduardo A. Robleto, Ronald E. Yasbin

Undergraduate Research Opportunities Program (UROP)

Here, we examine mutagenic programs that are independent of growth, such aspects of the evolutionary process are novel and have been implicated in the formation of cancers in animal cells and the acquisition of antibiotic resistance in animal pathogens. Adaptive or stationary phase mutagenesis is a genetic program to in increase diversity in cells under conditions of stress whereby cells escape non-dividing conditions. Previous research has shown that recombination functions are required to generate mutations that promote growth in Escherichia coli cells starved for carbon. This project tests the hypothesis that recombination functions are required for the generation of mutations …

Differential Localization Of Icsa And Icsp In The Outer Membrane Of Shigella Flexneri, Robin Herlands, Helen Wing

Undergraduate Research Opportunities Program (UROP)

Our goal is to visualize IcsP on the surface of S. flexneri and determine how its localization changes during growth versus stationary phases. We hypothesize IcsP will be present on the surface of S. flexneri differentially during growth and stationary phases of cell cycle. We hypothesize greater levels of IcsP seen during stationary phase (simultaneous with lower, more unipolar IcsA, due to IcsP mediated cleavage). During growth phase, we expect less IcsP to be present on the surface, and IcsA to be more evenly distributed around the cell surface.

Martian Life Detection With Amino Acid Enantiomers, Ali Jamil, Gaosen Zhang, Henry J. Sun

Undergraduate Research Opportunities Program (UROP)

The Viking mission showed that Martian soil can degrade a heterotrophic medium to carbon dioxide as if live microorganisms were present. The result is considered inconclusive, however, because abiotic oxidants, such as superoxides, may also exist on Mars and would explain the Viking result. One way to resolve this ambiguity is to repeat the Viking experiment with a isomerically pure medium. The consumption of one isomer, either D or L, would indicate biological activity. Indiscriminate destruction of both isomers would indicate abiotic redox processes. This idea was validated for glucose by REU research last summer (Sun et al. 2009). The …

Manipulation Of Foraging Rate In Honey Bees In A Natural Setting, Loann K.L. Larsen, Joyce H. Pang, Andrew Ammons, Michelle M. Elekonich

Undergraduate Research Opportunities Program (UROP)

The accumulation of reactive oxygen species (ROS) and free radicals within tissues creates oxidative stress, causing damage and eventual aging in an organism. Intense activity can increase the level of oxidative stress that occurs within an organism. In honey bees, this activity occurs during foraging flights. To measure levels of oxidative stress resulting from low and high foraging activity, we set up three colonies: a colony with a pollen trap to cause increased foraging, a normal colony (control), and a colony with an artificial waterfall to limit foraging. We counted flights of marked foragers at each colony for 5 to …

The Regulation Of The Icsp Promoter Of Shigella Flexneri By Ryhb, Lia A. Africa, Helen J. Wing

Undergraduate Research Opportunities Program (UROP)

Iron is an essential element in the metabolism of many organisms, including bacteria. In many pathogenic bacteria, the levels of iron present trigger the expression of many virulence genes. In Shigella, a gram-negative bacterium that causes dysentery in humans, the expression of a small regulatory RNA, ryhB, is blocked in the presence of iron. Studies have revealed that ryhB represses virB, a global regulator of virulence genes in Shigella.

The icsP gene is under the direct control of VirB. icsP encodes an outer membrane protease that cleaves a protein necessary for the actin tail assembly of Shigella in vitro. In …

Expression Of An Alternate Splice Form Of Bmi-1 In Multiple Myeloma, Adam Austin, Kristine Veys, Debbie Wong, James Tung

Undergraduate Research Opportunities Program (UROP)

The concept of “tumor stem cells” has garnered much attention in the last few years. Tumor stem cells are believed to exist among a heterogeneous group of cells that constitute a tumor. These tumor stem cells often express genes that are important for stem cell function, cell division, and maintenance of pluripotent state in stem cells. Stem cell or stem cell maintenance genes such as SALL 4 and Bmi-1 are often seen in these cancer cells and contribute to self-renewing divisions and cancer cell survival. In particular, high expression of Bmi-1 (B lymphoma mouse Moloney leukemia virus insertion region), a …

Survival Of Shigella Flexneri In Swimming Pool Water, Monique Gomez, Helen J. Wing

Undergraduate Research Opportunities Program (UROP)

Shigellosis is a disease caused by the pathogenic bacterium Shigella flexneri. The bacterium causes bloody diarrheadiarrhea, fever and abdominal pain. Infections can be and may cause fatal. . The disease may also cause bloody diarrhea and intense intestinal crampings. In the United States, there have been many outbreaks of shigellosis have been traced back to associated with inadequately disinfectedchlorinated public swimming pools and wading pools. These outbreaks of sShigellosis are most commonly seen among small children who play in these pools. . My goal is to determine how well Shigella survives in freshly chlorinated pool water and in pool water …

Germination And Characterization Of Bacillus Anthracis And Bacillus Cereus, Ann Desdemonia Nde-Neh Fowajuh, Ernesto Abel-Santos

Undergraduate Research Opportunities Program (UROP)

Bacillus cereus and Bacillus anthracis are micro organisms found in soil. Normally, only their spores are found in soil. We recently showed that, B. anthracis and B. cereus do not germinate in soil. Thus, how does B. cereus and B. anthracis continue their life cycle if they can not replicate in soil? We hypothesize that B. cereus and B. anthracis spores may germinate in the gut of nematodes. Caenorhabditis elegans was used as our model nematode to investigate this possibility. Subsequently, the goal of our research is to determine the effect of C. elegans on the life cycle of B. …

The Characterization Of Two Diverse Magnetotactic Bacteria: Lems And Mms-1, Ulysses C. Pickard, Paul A. Howse, Dennis A. Bazylinski

Undergraduate Research Opportunities Program (UROP)

Magnetotactic bacteria (MTB) are a diverse group of prokaryotes that biomineralize membrane-bound magnetic crystals known as magnetosomes. The magnetosomes are aligned within the cell and consist of either magnetite (Fe3O4) or greigite (Fe3S4). The biomineralization of magnetosomes consists of several processes including: invagination of the cytoplasmic membrane, iron uptake into the cell and then into the magnetosome membrane vesicle, and crystallization of the mineral phase inside the vesicle. Mam genes control magnetosome biomineralization with most of the genes present in an island called a magnetosome island. Many of the mam genes are conserved between different species of MTB. The genes …

Dna Secondary Structures And Their Contribution To Mutagenesis In B. Subtilis Stationary Phase Cells, Carmen Vallin, Katherine Ona, Chris Ross, Ronald E. Yasbin, Eduardo A. Robleto

Undergraduate Research Opportunities Program (UROP)

It is widely known and accepted that the cause of many mutations in cells are generated during the replication process of actively dividing cells, however more recent research has shown that mutations also arise in non growing conditions, a phenomenon known stationary phase mutagenesis. Much of what is known come from studies in eukaryotic and bacterial models. It is proposed that in nongrowing cells, the process of transcription plays an important role in mutagenesis. I will test the hypothesis that secondary structures formed of DNA generated transcription promote mutagenesis. The sequences transcriptiongenerated structures are speculated to be prone to mutations …