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Effects Of Oocyte Source On The Developmental Competence Of In Vitro Matured Goat Oocytes Fertilized By The Intracytoplasmic Sperm Injection Technique, Abu Nasar Md. Aminoor Rahman, Ramli Bin Abdullah, Wan Embong Wan-Khadijah
Effects Of Oocyte Source On The Developmental Competence Of In Vitro Matured Goat Oocytes Fertilized By The Intracytoplasmic Sperm Injection Technique, Abu Nasar Md. Aminoor Rahman, Ramli Bin Abdullah, Wan Embong Wan-Khadijah
Turkish Journal of Veterinary & Animal Sciences
This study evaluated the effects of oocyte source on in vitro maturation (IVM) and embryo developmental competence in goats by the intracytoplasmic sperm injection (ICSI) technique. Oocytes were obtained either by laparoscopic ovum pick-up (LOPU) or by slicing of abattoir ovaries. The donor does used for LOPU were superstimulated and their estrus was synchronized. Recovered oocytes from both sources were cultured in microdrops of IVM medium in the presence of CO2 (5%) at 38.5 °C in a CO2 incubator for 27 h. The ICSI was performed with immobilized sperm on the lid of a culture dish (35 mm) under an …