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2009

Electronic Theses and Dissertations

Oceanography

Mya arenaria Maine

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Full-Text Articles in Life Sciences

Investigating Saxitoxin Resistance In Softshell Clams (Mya Arenaria): Patterns Of Inheritance And Improvements On Methodology For Tracking And Identification, Scott A. Hamilton Dec 2009

Investigating Saxitoxin Resistance In Softshell Clams (Mya Arenaria): Patterns Of Inheritance And Improvements On Methodology For Tracking And Identification, Scott A. Hamilton

Electronic Theses and Dissertations

Attempts to characterize and study the population dynamics of the softshell clam Mya arenaria in relation to a mutation which confers resistance to paralytic shellfish toxins are complicated by a lack of non-lethal genotyping techniques, reliable tagging methods and an understanding of the inheritance patterns of the marker. Presented here, is a straightforward and non-lethal technique for clam genotyping, a new method for the long term tagging of clams, and the offspring genotype frequencies from a number of pair matings between clams of known genotype. Hemolymph extracted from M. arenaria was used directly in a polymerase chain reaction (PCR) to …