Life Sciences Commons^™

Genetic Relationship Between Longevity And Objectively Or Subjectively Assessed Performance Traits In Sheep Using Linear Censored Models, W. Mekkaway, Rainer Roehe, Ronald M. Lewis, M. H. Davies, L. Bünger, G. Simm, W. Haresign

Department of Animal Science: Faculty Publications

Genetic parameters of longevity in crossbred mule ewes, and genetic relationships among longevity, growth, body composition, and subjectively assessed traits on mule lambs and ewes have been estimated using Bayesian linear censored models. Additionally, the genetic associations between longevity and culling reasons were examined. Data comprised 1,797 observations of mule ewes for longevity, culling reasons, growth, body composition, mouth scores, and type traits. Longevity was defined as the time (in years) from 2 yr of age (the age at first lambing of most ewes) to culling or death. Censored data (i.e., observations for which only the lower bound of the …

Predictors Of Response And Genetic Resistance/Susceptibility In Pigs To Infection With Porcine Reproductive And Respiratory Syndrome Virus, Donald G. Levis, Rodger K. Johnson,, Thomas A. Rathje, Fernando A. Osorio

Department of Animal Science: Faculty Publications

The primary objective of this study was to determine differences in growth rate and expression of specific immune function genes and levels of cytokines between pigs that are more resistant and more susceptible to PRRSV infection. The data generated in this replication were combined with data from a previous replication. At 34 ± 5 days of age (8.2 ± 1.8 kg body weight), 220 weaned pigs free of PRRSV were transported from their farm of origin to the wean-to-finish barn at the Haskell Agricultural Laboratory. The pigs were randomly allotted to one of 16 pens (2.4 m x 4.3 m) …

Multiple Genes On Chromosome 7 Regulate Dopaminergic Amacrine Cell Number In The Mouse Retina, Irene E. Whitney, Mary A. Day, Daniel C. Ciobanu, Robert W. Williams, Benjamin E. Reese

Department of Animal Science: Faculty Publications

Purpose—The size of neuronal populations is modulated by gene variants that influence cell production and survival, in turn influencing neuronal connectivity, function, and disease risk. The size of the dopaminergic amacrine (DA) cell population is a highly heritable trait exhibiting six-fold variation among inbred strains of mice, and is used here to identify genes that modulate the number of DA cells.

Methods—The entire population was counted in retinal wholemounts from 37 genetically defined lines of mice, including six standard inbred strains, 25 recombinant inbred strains (AXB/BXA), reciprocal F1 hybrids, a chromosome (Chr) 7 consomic line, and three additional …

Nutrient And Fiber Type Relationships In Barrosã Muscles, P. Costa, L. C. Roseiro, V. Alves, Chris R. Calkins, R. J. B. Bessa, C. Santos

Department of Animal Science: Faculty Publications

Fiber type and nutrient compositions from Biceps femoris (Bf), Longissimus dorsi (Ld) and Supraspinatus (Ss) muscles of 10 Barrosã calves were determined and the respective relationships investigated by principal component analysis (PCA). The Ss muscle had higher proportion of type IIA fibers than Bf (P < 0.001) and Ld (P < 0.01), higher percentage of oxidative fibers than Ld muscle (P < 0.01) and lower proportion of IIB fibers than Bf (P P < 0.01). The latter muscle had higher intramuscular total lipids (ITL) (P P c9t11-CLA (P < 0.001), saturated fatty acids (SFA) (P < trans fatty acids (TFA) (P P P < n-6 PUFA (P < n-3 PUFA (P < 0.01), polyunsaturated/saturated fatty ac¬ids ratio (P/S) (P < 0.001) and hypocholesterolaemic/hypercholesterolaemic fatty acid ratio (h/H) (P P < and phospholipids (PL) (P < 0.05) contents were found in Ss muscle. Cholesterol amount was unaffected by anatomical location and ranged from 0.50 to 0.54 mg/g. Barrosã muscles were not well dis¬tinguishable by PCA since, based on fiber and nutritional compositions, they did not clearly present different pat¬terns. Barrosã-PDO veal is a low fat meat providing 367–558 mg of n-6 PUFA and 151-172 mg of n-3 PUFA of which 67–78 mg are EPA + DHA (based on 150 g consumption). The n-6 …

Evaluation Of Antral Follicle Count And Ovarian Morphology In Crossbred Beef Cows: Investigation Of Influence Of Stage Of The Estrous Cycle, Age, And Birth Weight, R. A. Cushman, M. F. Allan, Larry A. Kuehn, W. M. Snelling, Andrea S. Cupp, H. C. Freetly

Department of Animal Science: Faculty Publications

Depletion of the ovarian reserve is associated with reproductive senescence in mammalian females, and there is a positive relationship between the size of the ovarian reserve and the number of antral follicles on the surface of the ovary. Therefore, we conducted a series of experiments to investigate the influence of stage of the estrous cycle, age, and birth weight on antral follicle counts (AFC) in beef cows and heifers. Pairs of ovaries were collected from crossbred beef cows at slaughter (n = 72) or at necropsy (n = 333; 0 to 11 yr of age); all visible antral follicles were …

Performance And Digestibility Characteristics Of Finishing Diets Containing Distillers Grains, Composites Of Corn Processing Coproducts, Or Supplemental Corn Oil, K. J. Vander Pol, Matt K. Luebbe, G. I. Crawford, Galen E. Erickson, Terry J. Klopfenstein

Department of Animal Science: Faculty Publications

Three experiments evaluated the lipids in distillers grains plus solubles compared with corn or other sources of lipid in finishing diets. Experiment 1 utilized 60 individually fed yearling heifers (349 ± 34 kg) fed treatments consisting of 0, 20, or 40% (DM basis) wet distillers grains plus solubles (WDGS), or 0, 2.5, or 5.0% (DM basis) corn oil in a finishing diet based on high-moisture corn (HMC) and dry-rolled corn (DRC). Cattle fed 20 and 40% WDGS had greater (P < 0.10) G:F than cattle fed 0% WDGS. Cattle fed the 5.0% corn oil had lower overall performance than cattle fed the other diets. Results from Exp. 1 indicated that adding fat from WDGS improves performance, whereas supplementing 5.0% corn oil depressed G:F, suggesting that the fat within WDGS is different than corn oil. Experiment 2 used 234 yearling steers (352 ± 16 kg) fed 1 of 5 treatments consisting of 20 or 40% (DM basis) dry distillers grains plus solubles (DDGS), 1.3% or 2.6% (DM basis) tallow, or HMC. All diets contained 20% (DM basis) wet corn gluten feed (WCGF) as a method of controlling acidosis. No differences between treatments for any performance parameters were observed in Exp. 2. The DDGS may be similar to tallow and HMC in finishing diets containing 20% WCGF. Experiment 3 used 5 Holstein steers equipped with ruminal and duodenal cannulas in a 5x5 Latin square design. Treatments were a 40% WDGS diet, 2 composites, one consisting of corn bran and corn gluten meal (COMP), and one consisting of corn bran, corn gluten meal, and corn oil (COMP + OIL), and 2 DRC-based diets supplemented with corn oil (CON + OIL) or not (CON). Cattle fed the WDGS diet had numerically lower rumen pH compared with cattle fed other treatments. Cattle fed WDGS had greater (P < 0.10) molar proportions of propionate, lower (P < 0.10) acetate:propionate ratios, greater (P < 0.10) total tract fat digestion, and a greater (P < 0.10) proportion of unsaturated fatty acids reaching the duodenum than cattle fed other treatments. Therefore, the higher energy value of WDGS compared with corn may be due to more propionate production, higher fat digestibility, and more unsaturated fatty acids reaching the duodenum.

Cell Biology Symposium: The Role Of Microrna In Cell Function, Andrea S. Cupp, J. Matthews, E. Huff-Lonergan, D. M. Spurlock, D. Mclean

Department of Animal Science: Faculty Publications

A new symposium was introduced at the Centennial Meeting 23 of the American Society of Animal Science (ASAS) meeting during the joint annual meeting of ASAS and the American Dairy Science Association in Indianapolis, Indiana, on July 8, 2008. The purpose of the Cell Biology Symposium is to bring scientists with cutting edge basic research on a particular theme to the ASAS meeting. In the future we hope to expand this symposium by including a poster session for ASAS participants and invited speakers to interact. This year the theme of the Cell Biology Symposium was “The Role of MicroRNA in …

Effects Of Chemical Composition Variation On The Dynamics Of Ruminal Fermentation And Biological Value Of Corn Milling (Co)Products, L. O. Tedeschi, Paul J. Kononoff, K. Karges, M. L. Gibson

Department of Animal Science: Faculty Publications

The objectives of this study were to evaluate the dynamics of gas production of several corn (co)products, to develop equations to predict the rate of ruminal fiber digestion, to estimate total digestible nutrients (TDN) and net energy for lactation (NE_L), and to assess the stochasticity of chemical composition and nutritive value variability. Four corn milling (co)products were evaluated in this study: high protein dried distillers grains (HP-DDG), corn bran (BRAN) and dehydrated germ (GERM), and a dried distillers grains plus soluble produced with a low-heat drying process (BPX). Alfalfa hay was used as an internal standard feed in …

Influence Of Corn Hybrid And Processing Method On Nutrient Digestibility, Finishing Performance, And Carcass Characteristics, F. W. Harrelson, Matt K. Luebbe, N. F. Meyer, Galen E. Erickson, Terry J. Klopfenstein, David S. Jackson, W. A. Fithian

Department of Animal Science: Faculty Publications

A finishing trial and a digestion trial were conducted to evaluate the effects of corn hybrid and processing method on nutrient digestibility, finishing performance, and carcass characteristics. A 2 × 5 factorial arrangement of treatments was used for both trials. Factors included 2 processing methods, dry-rolled (DRC) or high-moisture (HMC), and 5 commercially available corn hybrids. The finishing trial (Exp. 1) utilized 475 yearling steers (379 ± 15 kg initial BW), stratified by BW into 2 blocks then assigned randomly to 1 of 60 pens (8 steers/pen). Treatments were assigned randomly to pens with 6 pens/treatment. Diets consisted of 67.5% …

Effect Of Essential Oils, Tylosin, And Monensin On Finishing Steer Performance, Carcass Characteristics, Liver Abscesses, Ruminal Fermentation, And Digestibility, N. F. Meyer, Galen E. Erickson, Terry J. Klopfenstein, Matthew A. Greenquist, Matt K. Luebbe, P. Williams, M. A. Engstrom

Department of Animal Science: Faculty Publications

A feedlot (Exp. 1) experiment was conducted to evaluate the effects of an essential oil mixture (EOM), experimental essential oil mixture (EXP), tylosin, and monensin (MON) on performance, carcass characteristics, and liver abscesses. A metabolism experiment (Exp. 2) was conducted to evaluate the effects of EOM, EXP, and MON on ruminal fermentation and digestibility in finishing steers. In Exp. 1, 468 yearling steers (398 ± 34 kg initial BW) were used in 50 pens (10 pens/treatment) and received their respective dietary treatments for 115 d. Five dietary treatments were compared in Exp. 1: 1) control, no additives (CON); 2) EOM, …

The Effect Of Feeding High-Protein Distillers Dried Grains On Milk Production Of Holstein Cows, K. J. Hubbard, Paul J. Kononoff, A. M. Gehman, J. M. Kelzer, K. Karges, M. L. Gibson

Department of Animal Science: Faculty Publications

The objectives of this study were to evaluate the effects of feeding high-protein distillers dried grains (HPDDG) on rumen degradability, dry matter intake, milk production, and milk composition. Sixteen lactating Holstein cows (12 multiparous and 4 primiparous) averaging 80 ± 14 d in milk were randomly assigned to 1 of 2 dietary treatments in a 2 × 2 crossover design. A portion of forage and all soy-based protein in the control diet were replaced by HPDDG (20% dry matter). Milk production and dry matter intake were recorded daily and averaged for d 19 to 21 of each 21-d period. Milk …

Winter Grazing System And Supplementation During Late Gestation Influence Performance Of Beef Cows And Steer Progeny, Daniel Larson, J. L. Martin, Don C. Adams, Richard N. Funston

Department of Animal Science: Faculty Publications

A 2 × 2 factorial study evaluated effects of cow wintering system and last trimester CP supplementation on performance of beef cows and steer progeny over a 3-yr period. Pregnant composite cows (Red Angus × Simmental) grazed winter range (WR; n = 4/yr) or corn residue (CR; n = 4/yr) during winter and within grazing treatment received 0.45 kg/d (DM) 28% CP cubes (PS; n = 4/yr) or no supplement (NS; n = 4/yr). Offspring steer calves entered the feedlot 14 d postweaning and were slaughtered 222 d later. Precalving BW was greater (P = 0.02) for PS than NS …

Multi-Tissue Coexpression Networks Reveal Unexpected Subnetworks Associated With Disease, Radu Dobrin, Jun Zhu, Cliona Molony, Carmen Argman, Mark L. Parish, Sonia Carlson, Mark F. Allan, Daniel Pomp, Eric E. Schadt

Department of Animal Science: Faculty Publications

Background: Obesity is a particularly complex disease that at least partially involves genetic and environmental perturbations to gene-networks connecting the hypothalamus and several metabolic tissues, resulting in an energy imbalance at the systems level.

Results: To provide an inter-tissue view of obesity with respect to molecular states that are associated with physiological states, we developed a framework for constructing tissue-to-tissue coexpression networks between genes in the hypothalamus, liver or adipose tissue. These networks have a scale-free architecture and are strikingly independent of gene-gene coexpression networks that are constructed from more standard analyses of single tissues. This is the first systematic …

Effects Of Ractopamine (Optaflexx) Fed In Combination With Melengestrol Acetate On Feedlot Heifer Performance, W. A. Griffin, G. E. Erickson, B. D. Dicke, T. J. Klopfenstein, R. J. Cooper, D. J. Jordon, R. S. Swingle, W. M. Moseley, G. E. Sides, D. J. Weigel

Department of Animal Science: Faculty Publications

Two commercial feedlot experiments were conducted to determine the effects of feeding melengestrol acetate (MGA) or MGA plus ractopamine (MGA+OPT) on the performance and carcass characteristics of finishing heifers. In Nebraska (Exp. 1), 1,807 heifers (337.3 ± 20.0 kg) and in Texas (Exp. 2), 1,964 heifers (331.5 ± 6.1 kg) were fed 0.4 mg of MGA daily. For heifers fed MGA+OPT, 200 mg of ractopamine was fed daily the last 29 (Exp. 2) or 36 d (Exp. 1). Live and carcass-adjusted performance data were collected. On a carcass-adjusted basis, G:F for the entire feeding period was improved (P < 0.01) by 1.7 and 3.7% in Exp. 1 and 2, respectively, for heifers fed MGA+OPT compared with MGA. For the last 29 to 36 d, G:F was increased (P < 0.02) by 8.1% (Exp. 1) or 27.2% (Exp. 2) on a carcass-adjusted basis for heifers fed MGA+OPT compared with MGA. Fat thickness, USDA YG, marbling score, LM area, and percentage USDA Choice were not different (P > 0.47) between …

Evaluation Of Revalor-Ih And Revalor-Is As Initial Implants Compared With Traditional Initial Implants For Finishing Heifers And Steers, J. D. Folmer, T. B. Farran, G. E. Erickson, T. J. Klopfenstein, C. D. Reinhardt, B. D. Dicke, J. S. Drouillard, M. N. Streeter, J. T. Vasconcelos

Department of Animal Science: Faculty Publications

Two commercial feedlot experiments were conducted to compare performance and carcass characteristics of feedlot heifers and steers using 2 implant strategies. In Exp. 1, heifers (n = 1,124; initial BW = 279 ± 5 kg) were administered either Revalor-IH [Intervet/ Schering Plough Animal Health, DeSoto, KS; 8 mg of estradiol (E2) and 80 mg of trenbolone acetate (TA)] or Synovex-H (Fort Dodge Animal Health, Overland Park, KS; 20 mg of estradiol benzoate and 200 mg of testosterone propionate) at initial processing, with both treatment groups receiving Revalor-200 (20 mg E2 and 200 mg TA) as the common terminal implant 81 …

Influence Of Corn Hybrid Traits And Processing Method On Nutrient Digestibility, Matt K. Luebbe, G. E. Erickson, T. J. Klopfenstein, W. A. Fithian

Department of Animal Science: Faculty Publications

Two experiments were conducted to determine the effects of corn hybrid and processing method on the site and extent of nutrient digestibility and ruminal fermentation. Treatments consisted of 2 processing methods, dry-rolled corn (DRC) and high-moisture corn (HMC), and 3 hybrids, H-8562 (1), 33P67 (2), and H-9230 (3), in a 2 × 3 factorial arrangement. In Exp. 1, six ruminally cannulated crossbred steers (436 ± 8 kg) were used in a 6 × 6 Latin square design. Total tract DM, OM, and starch digestibility (STD) were greater (P < 0.10) for HMC compared with DRC. A hybrid × processing method interaction (P < 0.10) was observed for propionate concentration and the acetate-to-propionate ratio. In Exp. 2, two ruminally and duodenally fistulated steers were used to determine the site and extent of nutrient digestion using the mobile bag technique at 2 ruminal incubation times. Ruminal STD was not different (P = 0.14) among hybrids but was greater (P < 0.01) for HMC compared with DRC. Postruminal and total tract STD were greater (P < 0.01) for hybrids 1 and 3 than for hybrid 2, and were greater (P < 0.01) for HMC than DRC. In both experiments the geometric mean diameter and geometric SD were influenced (P < 0.05) by both hybrid and processing method. More intense processing methods or selection of hybrids with softer kernels will result in greater digestibility and ruminal propionate concentrations. Digestibility of corn samples can be characterized using the mobile bag technique but particle size can influence results.

The Relationship Between Acid Detergent Insoluble Nitrogen And Nitrogen Digestibility In Lactating Dairy Cattle, K. J. Machacek, P. J. Kononoff

Department of Animal Science: Faculty Publications

Five trials (19 treatments) conducted at the University of Nebraska-Lincoln (UNL) on lactating dairy cattle were analyzed to determine how the concentration of ADIN in the ration affects total tract N digestibility. Additionally, 6 published studies (13 treatments) were included to expand the data set. Results from the UNL trials showed that as the concentration of ADIN in the ration increased, the digestibility of ADIN also increased. However, the relationship was poor (r² = 0.29). To account for random effects among trials, a meta-analysis was conducted. In the UNL trials, as the ration concentration of ADIN increased, total tract …

Comparison Of Feeding Wet Distillers Grains In A Bunk Or On The Ground To Cattle Grazing Native Sandhills Winter Range, Jacqueline Musgrave, Leslie Aaron Stalker, Matthew C. Stockton, T. J. Klopfenstein

Department of Animal Science: Faculty Publications

Two-experiments determined the effects of feeding wet distillers grains with solubles (WDGS) either on the ground or in a bunk to cattle grazing native Sandhills winter range. In Experiment 1 (Exp. 1), 120 multiparous March-calving cows (536 ± 53.5 kg BW) were stratified by age and assigned to one of four treatments: WDGS fed on the ground, either three or six d/wk; or WDGS fed in a bunk either three or six d/wk. In Experiment 2 (Exp. 2), 63 March-born steer calves (201.2 ± 27.5 kg BW) were stratified by weight and assigned to one of two feeding treatments: WDGS …

Supplementing Wet Distillers Grains Mixed With Low Quality Forage To Grazing Cow Calf Pairs, Brandon L. Nuttelman, William Griffin, T. J. Klopfenstein, Walter H. Schacht, Leslie Aaron Stalker, Jacqueline Musgrave, Jerry D. Volesky

Department of Animal Science: Faculty Publications

Two consecutive summer grazing studies were conducted to quantify the effect of supplementing cows with wet distillers grains (WDGS) mixed with low quality forage on 1) grazed forage intake and 2) cow and calf performance. During exp. 1, twenty-four three year old lactating cows rotationally grazed for 56 d and were assigned to one of three treatments: 1) the recommended stocking rate of 1.48 AUM/ha with no supplementation (CON1), 2) double the recommended stocking rate (2.96 AUM/ha) and supplemented 6.64 kg/hd daily of 45% grass hay and 55% WDGS (DM) to replace 50% of estimated total intake (SUP), and 3) …

Large-Scale Association Study For Structural Soundness And Leg Locomotion Traits In The Pig, Bin Fan, Suneel K. Onteru, Benny E. Mote, Timo Serenius, Kenneth J. Stalder, Max F. Rothschild

Department of Animal Science: Faculty Publications

Background: Identification and culling of replacement gilts with poor skeletal conformation and feet and leg (FL) unsoundness is an approach used to reduce sow culling and mortality rates in breeding stock. Few candidate genes related to soundness traits have been identified in the pig.

Methods: In this study, 2066 commercial females were scored for 17 traits describing body conformation and FL structure, and were used for association analyses. Genotyping of 121 SNPs derived from 95 genes was implemented using Sequenom's MassARRAY system.

Results: Based on the association results from single trait and principal components using mixed linear …

Association And Haplotype Analyses Of Positional Candidate Genes In Five Genomic Regions Linked To Scrotal Hernia In Commercial Pig Lines, Zhi-Qiang Du, Xia Zhao, Natascha Vukasinovic, Fernanda Rodriguez, Archie C. Clutter, Max F. Rothschild

Department of Animal Science: Faculty Publications

Scrotal hernia in pigs is a complex trait likely affected by genetic and environmental factors. A large-scale association analysis of positional and functional candidate genes was conducted in four previously identified genomic regions linked to hernia susceptibility on Sus scrofa chromosomes 2 and 12, as well as the fifth region around 67 cM on chromosome 2, respectively. In total, 151 out of 416 SNPs discovered were genotyped successfully. Using a family-based analysis we found that four regions surrounding ELF5, KIF18A, COL23A1 on chromosome 2, and NPTX1 on chromosome 12, respectively, may contain the genetic variants important for the …

Age And Diet Affect Gene Expression Profile In Canine Skeletal Muscle, Ingmar S. Middelbos, Brittany M. Vester, Lisa K. Karr-Lilienthal, Lawrence B. Schook, Kelly S. Swanson

Department of Animal Science: Faculty Publications

We evaluated gene transcription in canine skeletal muscle (biceps femoris) using microarray analysis to identify effects of age and diet on gene expression. Twelve female beagles were used (six 1-year olds and six 12-year olds) and they were fed one of two experimental diets for 12 months. One diet contained primarily plant-based protein sources (PPB), whereas the second diet contained primarily animal-based protein sources (APB). Affymetrix GeneChip Canine Genome Arrays were used to hybridize extracted RNA. Age had the greatest effect on gene transcription (262 differentially expressed genes), whereas the effect of diet was relatively small (22 differentially expressed genes). …

Characterization Of 24 Microsatellite Loci In Delta Smelt, Hypomesus Transpacificus, And Their Cross-Species Amplification In Two Other Smelt Species Of The Osmeridae Family, Kathleen M. Fisch, Jessica Lynn Petersen, Melinda R. Baerwald, John K. Pedroia, Bernie May

Department of Animal Science: Faculty Publications

We characterized 24 polymorphic tetranucleotide microsatellite loci for delta smelt (Hypomesus transpacificus) endemic to the San Francisco Bay Estuary, California, USA. Screening of samples (n = 30) yielded two to 26 alleles per locus with observed levels of heterozygosity ranging from 0.17 to 1.0. Only one locus deviated from Hardy–Weinberg equilibrium, suggesting these individuals originate from a single panmictic population. Linkage disequilibrium was found in two pairs of loci after excluding the locus out of Hardy–Weinberg equilibrium. Twenty-two primer pairs cross-amplified in wakasagi smelt (Hypomesus nipponensis), and 15 primer pairs cross-amplified in longfin smelt ( …

Effects Of Social Interactions On Empirical Responses To Selection For Average Daily Gain Of Boars, C. Y. Chen, Rodger K. Johnson, S. Newman, Stephen D. Kachman, L. Dale Van Vleck

Department of Animal Science: Faculty Publications

Effects of social interactions on responses to selection for ADG were examined with records of 9,720 boars from dam lines (1 and 2) and sire lines (3 and 4) provided by Pig Improvement Company. Each line was analyzed separately. Pens contained 15 boars. Gains (ADG) were measured from about 71 to 161 d of age and weight from 31 to 120 kg. Models included fixed effects of contemporary groups and initial test age as a covariate, and random direct genetic (d), social genetic (c), social environmental (ce), and litter (lt) effects. Estimates of direct heritability with Model 1 (the full …

Additional Files For “Multi-Tissue Coexpression Networks Reveal Unexpected Subnetworks Associated With Disease”, Radu Dobrin, Jun Zhu, Cliona Molony, Carmen Argman, Mark L. Parrish, Sonia Carlson, Mark F. Allan, Daniel Pomp, Eric E. Schadt

Department of Animal Science: Faculty Publications

Figure S1 shows the connectivity distribution P(k) for GGC networks. Figure S2 shows FDR curves for each tissue in the analysis. Figure S3 shows modules in single tissue GGC networks as detected by the algorithm. Each cluster is marked by the yellow rectangles. Figure S4 shows single tissue module enrichment. Each panel has the following structure: top, P-values from FET for cis-eQTL blue bars over- and red bars under-enriched; middle, percentage overlap between module and genes with cis-eQTL; bottom, percentage overlap between each module and genes on each chromosome. The scale is between green and black where green represents 0% …

Effects Of Feeding Three Types Of Corn-Milling Coproducts On Milk Production And Ruminal Fermentation Of Lactating Holstein Cattle, J. M. Kelzer, Paul J. Kononoff, A. M. Gehman, L. O. Tedeschi, K. Karges, M. L. Gibson

Department of Animal Science: Faculty Publications

Two experiments were conducted to determine the effects of feeding 3 corn-milling coproducts on intake, milk production, ruminal fermentation, and digestibility of lactating Holstein cows. In experiment 1, three corn-milling coproducts were fed at 15% of the diet dry matter (DM) to 28 Holstein cows averaging (±SD) 625 ± 81 kg of body weight and 116 ± 33 d in milk to determine effects on DM intake and milk production. In experiment 2, the same rations were fed to 4 ruminally fistulated, multiparous Holstein cows averaging 677 ± 41 kg of body weight and 144 ± 5 d in milk …

Evaluation Of Antral Follicle Count And Ovarian Morphology In Crossbred Beef Cows: Investigation Of Influence Of Stage Of The Estrous Cycle, Age, And Birth Weight, R. A. Cushman, M. F. Allan, L. A. Kuehn, W. M. Snelling, Andrea S. Cupp, H. C. Freetly

Department of Animal Science: Faculty Publications

Depletion of the ovarian reserve is associated with reproductive senescence in mammalian females, and there is a positive relationship between the size of the ovarian reserve and the number of antral follicles on the surface of the ovary. Therefore, we conducted a series of experiments to investigate the influence of stage of the estrous cycle, age, and birth weight on antral follicle counts (AFC) in beef cows and heifers. Pairs of ovaries were collected from crossbred beef cows at slaughter (n = 72) or at necropsy (n = 333; 0 to 11 yr of age); all visible antral follicles were …

Effects Of Sodium Chloride And Fat Supplementation On Finishing Steers Exposed To Hot And Cold Conditions, J. B. Gaughan, Terry L. Mader

Department of Animal Science: Faculty Publications

Three studies were conducted to evaluate the effects of supplemental fat and salt (sodium chloride) on DMI, daily water intake (DWI), body temperature, and respiration rate (RR) in Bos Taurus beef cattle. In Exp. 1 and 2, whole soybeans (SB) were used as the supplemental fat source. In Exp. 3, palm kernel meal and tallow were used. Experiment 1 (winter) and Exp. 2 (summer) were undertaken in an outside feedlot. Experiment 3 was conducted in a climate-controlled facility (mean ambient temperature = 29.9°C). In Exp. 1, three diets, 1) control; 2) salt (control + 1% sodium chloride); and 3) salt-SB …

Board-Invited Review: Porcine Mucosal Immunity Of The Gastrointestinal Tract, Thomas E. Burkey, K. A. Skjolaas, J. E. Minton

Department of Animal Science: Faculty Publications

The gastrointestinal tract (GIT) constitutes one of the largest immunological organs of the body. The GIT must permit absorption of nutrients while also maintaining the ability to respond appropriately to a diverse milieu of dietary and microbial antigenic components. Because of the diverse population of antigenic components within the GIT, a sophisticated mucosal immune system has evolved that relies on collaboration between the innate and adaptive arms of immunity. The collaborative, mucosal immune effort offers protection from harmful pathogens while also being tolerant of dietary antigens and normal microbial flora. Knowledge with respect to porcine mucosal immunity is important as …

Effect Of Sorting And Optaflexx Supplementation On Feedlot Performance And Profitability Of Long Yearling Steers, W. A. Griffin, T. J. Klopfenstein, G. E. Erickson, D. M. Feuz, K. J. Vander Pol, Matthew A. Greenquist

Department of Animal Science: Faculty Publications

A 2-yr study was conducted using 200 long yearling steers/yr (436 ± 30 kg) to determine the effect on performance and economics of sorting by BW at feedlot entry and feeding 200 mg/steer of Optaflexx (OPT) daily for the last 28 d. At feedlot entry, steers were allotted into 1 of 4 treatments in a 2 × 2 factorial arrangement: sorted or unsorted with or without OPT. Sorted steers were placed into 1 of 3 groups—1) heavy steers (32%; 468 kg), 2) medium steers (44%; 432 kg), or 3) light steers (24%; 399 kg)—and were fed for 97, 118, or …