Life Sciences Commons^™

Ppar Agonists Down-Regulate The Expression Of Atp10c Mrna During Adipogenesis, A Peretich, Maria Cekanova Ms, Rndr, Phd, S Hurst, Sj Baek, Madhu Dahr

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

No abstract provided.

Morphogenesis Signaling Components Influence Cell Cycle Regulation By Cyclin Dependent Kinase, Brian Td Tobe, Ana A. Kitazono, Jacqueline S. Garcia, Renee A. Garber, Brooke J. Bevis, John S. Choy, Daniel Chasman, Stephen J. Kron

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

Background

The yeast cell cycle is largely controlled by the cyclin-dependent kinase (CDK) Cdc28. Recent evidence suggests that both CDK complex stability as well as function during mitosis is determined by precise regulation of Swe1, a CDK inhibitory kinase and cyclin binding partner. A model of mitotic progression has been provided by study of filamentous yeast. When facing nutrient-limited conditions, Ras2-mediated PKA and MAPK signaling cascades induce a switch from round to filamentous morphology resulting in delayed mitotic progression.

Results

To delineate how the dimorphic switch contributes to cell cycle regulation, temperature sensitive cdc28 mutants exhibiting constitutive filamentation were subjected …

The Fast Technique: A Simplified Agrobacterium-Based Transformation Method For Transient Gene Expression Analysis In Seedlings Of Arabidopsis And Other Plant Species, Jian-Feng Li, Eunsook Park, Albrecht G. Von Arnim, Andreas Nebenführ

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

Background

Plant genome sequencing has resulted in the identification of a large number of uncharacterized genes. To investigate these unknown gene functions, several transient transformation systems have been developed as quick and convenient alternatives to the lengthy transgenic assay. These transient assays include biolistic bombardment, protoplast transfection and Agrobacterium-mediated transient transformation, each having advantages and disadvantages depending on the research purposes.

Results

We present a novel transient assay based on cocultivation of young Arabidopsis (Arabidopsis thaliana) seedlings with Agrobacterium tumefaciens in the presence of a surfactant which does not require any dedicated equipment and can be carried …

The Fast Technique: A Simplified Agrobacterium-Based Transformation Method For Transient Gene Expression Analysis In Seedlings Of Arabidopsis And Other Plant Species, Jian-Feng Li, Eunsook Park, Albrecht G. Von Arnim, Andreas Nebenführ

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

Background

Plant genome sequencing has resulted in the identification of a large number of uncharacterized genes. To investigate these unknown gene functions, several transient transformation systems have been developed as quick and convenient alternatives to the lengthy transgenic assay. These transient assays include biolistic bombardment, protoplast transfection and Agrobacterium-mediated transient transformation, each having advantages and disadvantages depending on the research purposes.

Results

We present a novel transient assay based on cocultivation of young Arabidopsis (Arabidopsis thaliana) seedlings with Agrobacterium tumefaciens in the presence of a surfactant which does not require any dedicated equipment and can be carried …