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Articles 1 - 8 of 8
Full-Text Articles in Life Sciences
Development Of Structured Delivery Systems Using Nanolaminated Biopolymer Layers, Young-Hee Cho
Development Of Structured Delivery Systems Using Nanolaminated Biopolymer Layers, Young-Hee Cho
Open Access Dissertations
The objectives of this study were to carry out research to better understand of the formation, stability and properties of multilayer emulsions containing nano-laminated biopolymer coatings, and to utilize this information to develop food-grade delivery systems. The effect of various preparation parameters on the formation and stability of multilayer emulsions was investigated: droplet concentration; mean droplet diameter; droplet charge; biopolymer concentration. β-lactoglobulin (β-Lg) stabilized emulsions (0.5 – 10 wt% oil) containing different pectin concentrations (0 to 0.5 wt%) were prepared at pH 7 (where lipid droplets and pectin molecules were both anionic) and pH 3.5 (where lipid droplets were cationic …
Fabrication Of Protein-Polysaccharide Particulates Through Thermal Treatment Of Associative Complexes, Owen Griffith Jones
Fabrication Of Protein-Polysaccharide Particulates Through Thermal Treatment Of Associative Complexes, Owen Griffith Jones
Open Access Dissertations
Mixed solutions of β-lactoglobulin and anionic polysaccharides, specifically pectin, were formed into associative complexes through pH reduction from neutral conditions. Thermal treatment of these associative complexes was investigated as a function of biopolymer composition, heating conditions, pH, and ionic strength. Thermal treatment of β-lactoglobulin-pectin complexes at pH 4.5 – 5.0 was found to create protein-based particulates of consistent and narrow size distribution (diameter ~ 150 – 400 nm). These particulates were relatively stable to further pH adjustment and to high levels of salt (200 NaCl). Particle characteristics were maintained after re-suspending them in aqueous solutions after they have been either …
Levels, Enterotoxigenicity, Growth And Physical Characterisitcs Of B. Cereus From U.S Retail Rice, Chandrakant R. Ankolekar
Levels, Enterotoxigenicity, Growth And Physical Characterisitcs Of B. Cereus From U.S Retail Rice, Chandrakant R. Ankolekar
Masters Theses 1911 - February 2014
Bacillus cereus is a ubiquitously found foodborne pathogen that is frequently associated with two types of illness: emesis and diarrhea. Two heat labile enterotoxins have been associated with the diarrheal syndrome whereas a heat stable acid stable peptide toxin has been associated with the emetic syndrome. In the U.S, B. cereus is responsible for 1-2% of the total outbreaks from bacteria. Although there are reports of isolation and characterization of this pathogen from various food stuffs all around the world, there are no reports on the levels, toxin producing ability, or growth characteristics from U.S retail rice. Considering that rice …
A Multiplexed Optofluidic Biomolecular Sensor For Low Mass Detection, Julie M. Goddard, S. Mandal, D. Erikson
A Multiplexed Optofluidic Biomolecular Sensor For Low Mass Detection, Julie M. Goddard, S. Mandal, D. Erikson
Julie M. Goddard
Optical techniques have proven to be well suited for in situ biomolecular sensing because they enable high fidelity measurements in aqueous environments, are minimally affected by background solution pH or ionic strength, and facilitate label-free detection. Recently, there has been significant interest in developing new classes of optically resonant biosensors possessing very high quality-factors. This high quality-factor enables them to resolve the presence of very small amounts of bound mass and leads to very low limits of detection. A drawback of these devices is that the majority of the resonant electromagnetic energy is confined within the solid light-guiding structure thus …
Synergistic Actions Of Atorvastatin With Γ-Tocotrienol And Celecoxib Against Human Colon Cancer Ht29 And Hct116 Cells, Hang Xiao, Z. Yang, H. Jin, D. Tsang, P. Koo, C.S. Yang
Synergistic Actions Of Atorvastatin With Γ-Tocotrienol And Celecoxib Against Human Colon Cancer Ht29 And Hct116 Cells, Hang Xiao, Z. Yang, H. Jin, D. Tsang, P. Koo, C.S. Yang
Hang Xiao
The synergistic actions of atorvastatin (ATST) with gamma-tocotrienol (gamma-TT) and celecoxib (CXIB) were studied in human colon cancer cell lines HT29 and HCT116. The synergistic inhibition of cell growth by ATST and gamma-TT was demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and isobologram analysis. delta-TT exhibited a similar inhibitory action when combined with ATST. Mevalonate and geranylgeranyl pyrophosphate eliminated most of the growth inhibitory effect of ATST, but only marginally decreased that of gamma-TT; whereas farnesyl pyrophosphate and squalene exhibited little effect on the inhibitory action of ATST and gamma-TT, indicating protein geranylgeranylation, but not farnesylation are involved in the inhibition …
Optically Resonant Nanophotonic Devices For Label-Free Biomolecular Detection, Julie M. Goddard, S. Mandal, D. Erickson
Optically Resonant Nanophotonic Devices For Label-Free Biomolecular Detection, Julie M. Goddard, S. Mandal, D. Erickson
Julie M. Goddard
Optical devices, such as surface plasmonresonance chips and waveguide-based Mach–Zehnder interferometers, have long been successfully used as label-free biomolecular sensors. Recently, however, there has been increased interest in developing new approaches to biomolecular detection that can improve on the limit of detection, specificity, and multiplexibility of these early devices and address emerging challenges in pathogen detection, disease diagnosis, and drug discovery. As we describe in this chapter, planar optically resonant nanophotonic devices (such as ring resonators, whispering gallery modes, and photonic crystal cavities) are one method that shows promise in significantly advancing the technology. Here we first provide a short …
Bioconjugation Techniques For Microfluidic Biosensors, Julie M. Goddard, D. Erickson
Bioconjugation Techniques For Microfluidic Biosensors, Julie M. Goddard, D. Erickson
Julie M. Goddard
We have evaluated five bioconjugation chemistries for immobilizing DNA onto silicon substrates for microfluidic biosensing applications. Conjugation by organosilanes is compared with linkage by carbonyldiimidazole (CDI) activation of silanol groups and utilization of dendrimers. Chemistries were compared in terms of immobilization and hybridization density, stability under microfluidic flow-induced shear stress, and stability after extended storage in aqueous solutions. Conjugation by dendrimer tether provided the greatest hybridization efficiency; however, conjugation by aminosilane treated with glutaraldehyde yielded the greatest immobilization and hybridization densities, as well as enhanced stability to both shear stress and extended storage in an aqueous environment. Direct linkage by …
A Biosensor Assay For The Detection Of Mycobacterium Avium Subsp. Paratuberculosis In Fecal Samples, V. Kumanan, Sam R. Nugen, A.J. Baeumner, Y.F. Chang
A Biosensor Assay For The Detection Of Mycobacterium Avium Subsp. Paratuberculosis In Fecal Samples, V. Kumanan, Sam R. Nugen, A.J. Baeumner, Y.F. Chang
Sam R. Nugen
A simple, membrane-strip-based lateral-flow (LF) biosensor assay and a high-throughput microtiter plate assay have been combined with a reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of a small number (ten) of viable Mycobacterium (M.) avium subsp. paratuberculosis (MAP) cells in fecal samples. The assays are based on the identification of the RNA of the IS900 element of MAP. For the assay, RNA was extracted from fecal samples spiked with a known quantity of (101 to 106) MAP cells and amplified using RT-PCR and identified by the LF biosensor and the microtiter plate assay. While the LF biosensor assay …