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Isolation Of A 3-Methylindole-Producing Bacterium From A Swine Waste Lagoon, Michelle L. Fusting
Isolation Of A 3-Methylindole-Producing Bacterium From A Swine Waste Lagoon, Michelle L. Fusting
Mahurin Honors College Capstone Experience/Thesis Projects
With an increase in animal production closer to populated areas, odorants from animal waste are a rapidly rising concern. 3-Methylindole (3-MI) is an odorant produced from the biodegradation of L-tryptophan by bacteria in animal waste. Currently, no 3-MI producing bacterium has been isolated from swine waste, and isolation of such an organism would provide insight into production and regulation of 3-MI. Therefore, an experiment was designed to isolate a 3-MI producer based on previous work which indicates the presence of Fe(III) increases 3-MI levels. An Enterococcus sp. was isolated from WKU's primary swine waste lagoon in rich, anaerobic medium plus …