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Characterization Of Volatile Organic Compounds In Bovine Breath Using Solid-Phase Microextraction And Gas Chromatography-Mass Spectrometry, Jacek A. Koziel, Jarett P. Spinhirne, Norbert K. Chirase
Characterization Of Volatile Organic Compounds In Bovine Breath Using Solid-Phase Microextraction And Gas Chromatography-Mass Spectrometry, Jacek A. Koziel, Jarett P. Spinhirne, Norbert K. Chirase
Jacek A. Koziel
A face mask sampling device, DVB/Carboxen/PDMS 50/30 µ m solid phase microextraction (SPME) fibers, and a gas chromatograph with a mass spectrometer detector (GC-MS) were used for sampling and analysis of volatile organic compounds (VOCs) in bovine breath. Breath of 3 morbid steers with respiratory tract infections and 3 healthy steers were sampled 7 times in 19 days for 15 min at each sampling. A total of 21 VOCs were detected, many of them for the first time in cattle breath. Preliminary statistical analyses using Chi-square Test on the frequency of detection of each VOC in each group was performed. …