Life Sciences Commons^™

Myasthenia Gravis-Like Syndrome Induced By Expression Of Interferon Gamma In The Neuromuscular Junction., Danling Gu, Lise Wogensen, Nigel A. Calcutt, Chunyao Xia, Simin Zhu, John P. Merlie, Howard S. Fox, Jon Lindstrom, Henry C. Powell, Nora Sarvetnick

Journal Articles: Regenerative Medicine

Abnormal humoral responses toward motor end plate constituents in muscle induce myasthenia gravis (MG). To study the etiology of this disease, and whether it could be induced by host defense molecules, we examined the consequences of interferon (IFN) gamma production within the neuromuscular junction of transgenic mice. The transgenic mice exhibited gradually increasing muscular weakness, flaccid paralysis, and functional disruption of the neuromuscular junction that was reversed after administration of an inhibitor of acetylcholinesterase, features which are strikingly similar to human MG. Furthermore, histological examination revealed infiltration of mononuclear cells and autoantibody deposition at motor end plates. Immunoprecipitation analysis indicated …

Sensitization To Self (Virus) Antigen By In Situ Expression Of Murine Interferon-Gamma., Myung-Shik Lee, Matthias Von Herrath, Hans Reiser, Michael B.A. Oldstone, Nora Sarvetnick

Journal Articles: Regenerative Medicine

Autoimmune disease results from inflammatory destruction of tissues by aberrant self-reactive lymphocytes. We studied the autoimmune potential of T lymphocytes immunologically ignorant of viral antigens acting as self antigens and whether the host defense molecule IFN-gamma could stimulate these cells to cytotoxic competency. For this purpose, we produced double transgenic mice expressing pancreatic IFN-gamma as well as lymphocytic choriomeningitis virus (LCMV) nucleoprotein (NP) or glycoprotein (GP) antigen. 100% of the NP+/IFN-gamma+ mice became diabetic before 2 mo of age, while none of the NP single transgenic littermates and only 10% of IFN-gamma single transgenic littermates did. Strikingly, NP+/IFN-gamma+ mice spontaneously …

G95-1244 Ventilation Fans: Efficiency And Maintenance, David P. Shelton, Gerald R. Bodman

University of Nebraska-Lincoln Extension: Historical Materials

This publication describes efficiency and design factors to consider when selecting a ventilation fan. Basic maintenance procedures needed for efficient operation are also discussed.

An energy efficient ventilation system requires good design and good equipment. Selecting efficient ventilation fans and keeping them in good working condition will keep operating costs down.

The Animal Research Controversy: Protest, Process & Public Policy, Andrew N. Rowan, Franklin M. Loew, Joan C. Weer

Experimentation Collection

The controversy today regarding the use of animals in research appears on the surface to be a strongly polarized struggle between the scientific community and the animal protection movement. However, there is a wide range of opinions and philosophies on both sides. Mistrust between the factions has blossomed while communication has withered. Through the 1960s, 1970s and early 1980s, the animal movement grew in numbers and financial resources, and developed much greater public recognition and political clout. The research community paid relatively little attention to the animal movement for much of this period but, alarmed by several public relations coups …

G95-1243 Ventilation Fans: Types And Sizes, Gerald R. Bodman, David P. Shelton

University of Nebraska-Lincoln Extension: Historical Materials

This publication describes the most common types of ventilation fans and compares their physical and performance characteristics.

Two general types of ventilation fans are commonly used for agricultural applications. Each type has many different blade diameters, materials, blade shapes, speeds, and motor horsepowers available. The desired airflow rate and the resistance to airflow through the ventilation system, or static pressure capabilities, are the two primary considerations when selecting a ventilation fan. Other considerations include maintenance requirements, noise levels, and energy efficiency.

G95-1253 Basic Principles Of Mastitis Control, Jeffrey F. Keown, Paul J. Kononoff

University of Nebraska-Lincoln Extension: Historical Materials

Mastitis in dairy cows is a frustrating, costly, and complex infection. A good, workable, effective preventive control program is presented in this NebGuide.

General Characteristics of Mastitis

Mastitis is an inflammation of the mammary gland usually caused by bacterial infection of udder tissues. Except for rare injuries, infectious bacteria or other pathogens, e.g. yeast, fungi, etc., enter the udder through the teat end. Mastitis in both clinical (obvious abnormality, visible to naked eye) and subclinical (unseen signs of abnormality, invisible to nake eye) stages is a frustrating, costly and complex disease that reduces the quality and quantity of milk.