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2023

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Full-Text Articles in Life Sciences

Consequences Of Spatial Structure In Soil–Geomorphic Data On The Results Of Machine Learning Models, Daehyun Kim, Insang Song, Lorrayne Miralha, Daniel R. Hirmas, Ryan W. Mcewan, Tom G. Mueller, Pavel Samonil Dec 2023

Consequences Of Spatial Structure In Soil–Geomorphic Data On The Results Of Machine Learning Models, Daehyun Kim, Insang Song, Lorrayne Miralha, Daniel R. Hirmas, Ryan W. Mcewan, Tom G. Mueller, Pavel Samonil

Biology Faculty Publications

In this paper, we examined the degree to which inherent spatial structure in soil properties influences the outcomes of machine learning (ML) approaches to predicting soil spatial variability. We compared the performances of four ML algorithms (support vector machine, artificial neural network, random forest, and random forest for spatial data) against two non-ML algorithms (ordinary least squares regression and spatial filtering regression). None of the ML algorithms produced residuals that had lower mean values or were less autocorrelated over space compared with the non-ML approaches. We recommend the use of random forest when a soil variable of interest is weakly …


Fungal Empyema Thoracis, A Rare But An Emerging Entity: A Retrospective Case Series From Pakistan, Nousheen Iqbal, Akbar Shoukat Ali, Aqusa Zahid, Kauser Jabeen, Muhammad Irfan Dec 2023

Fungal Empyema Thoracis, A Rare But An Emerging Entity: A Retrospective Case Series From Pakistan, Nousheen Iqbal, Akbar Shoukat Ali, Aqusa Zahid, Kauser Jabeen, Muhammad Irfan

Section of Pulmonary & Critical Care

Background and objective: Fungal empyema is a rare entity which is associated with high mortality. It is mostly seen in immune-compromised hosts. However, there is limited data available on fungal empyema from developing countries regarding risk factors, treatment, and outcome. This study was conducted to determine the risk factors, clinical features, treatment, and outcome of fungal empyema.
Methods: A retrospective observational study was performed on proven fungal empyema cases, admitted at Aga Khan University Hospital, Karachi, Pakistan during January 2018 to May 2021. We excluded all those patients with polymicrobial bacterial and fungal empyema or with negative pleural fluid cultures. …


Anti-Apostatic Feeding Behavior In Two Species Of Assassin Bug, The White-Spotted Assassin Bug Platymeris Biguttatus And Red-Spotted Assassin Bug Platymeris Rhadamanthus (Hemiptera: Reduviidae), Julia H. Potocnjak, Christopher Vitek Dec 2023

Anti-Apostatic Feeding Behavior In Two Species Of Assassin Bug, The White-Spotted Assassin Bug Platymeris Biguttatus And Red-Spotted Assassin Bug Platymeris Rhadamanthus (Hemiptera: Reduviidae), Julia H. Potocnjak, Christopher Vitek

School of Integrative Biological and Chemical Sciences Faculty Publications and Presentations

Predator-prey relationships are a driving force when examining community ecology. One question in predator-prey interactions is how a predator may respond to novel prey phenotypes, a form of frequency dependent prey selection. A predator may selectively target or avoid the novel prey, a behavior referred to as anti-apostatic or apostatic selection respectively. We examined apostatic prey selection by observing feeding behavior in two species of assassin bugs (Hemiptera: Reduviidae), the white-spotted assassin bug (Platymeris biguttatus) and the red-spotted assassin bug (Platymeris rhadamanthus) which were fed unmodified and modified (novel) phenotypes of Turkistan cockroaches, Blatta lateralis (Blattodea: …


Melittological Notes From Guatemala (Hymenoptera: Anthophila), 1. New Country Records For Centris Fabricius (Apidae) And Zikanapis Moure (Colletidae), Silas Bossert, José Monzón-Sierra, Richard S. Zack, Ana Cristina García, Elizabeth A. Murray Dec 2023

Melittological Notes From Guatemala (Hymenoptera: Anthophila), 1. New Country Records For Centris Fabricius (Apidae) And Zikanapis Moure (Colletidae), Silas Bossert, José Monzón-Sierra, Richard S. Zack, Ana Cristina García, Elizabeth A. Murray

Insecta Mundi

Abstract

Three species of bees (Hymenoptera: Apidae, Colletidae) are newly recorded for Guatemala: Centris obscurior Michener, Centris vidua Mocsáry, and Zikanapis inbio (Michener, Engel and Ayala). We discuss aspects of their biology and circumstances of the collecting events and provide information on their presently known distribution.

Resumen

Se registran por primera vez tres especies de abejas (HymenopteraN: Apidae, Colletidae) para Guatemala: Centris obscurior Michener, Centris vidua Mocsáry, y Zikanapis inbio (Michener, Engel y Ayala). Discutimos aspectos de su biología, circunstancias bajo las cuáles fueron colectadas y se provee información en la distribución conocida actualmente.


A Revision Of Dyscolotaxia Horn, 1890 (Coleoptera: Eucnemidae: Macraulacinae: Macraulacini), With The Description Of Amazotaxia New Genus From South America, Robert L. Otto Dec 2023

A Revision Of Dyscolotaxia Horn, 1890 (Coleoptera: Eucnemidae: Macraulacinae: Macraulacini), With The Description Of Amazotaxia New Genus From South America, Robert L. Otto

Insecta Mundi

Abstract

The Neotropical eucnemid genus, Dyscolotaxia Horn, is revised following the examination of specimens from five collections, examination of the published original description of Plesiofornax tetratoma Chassain, and from images provided by Fernanda Salazar-Buenaño (QCAZI). Dyscolotaxia championi Horn is redescribed with included descriptions of the male genitalia and a female specimen. Three new species of false click beetles (Coleoptera: Eucnemidae) are described from the Neotropical region. These new species are: Dyscolotaxia chiriquiensis (Panama), Dyscolotaxia hispaniolensis (Dominican Republic), and Dyscolotaxia picea (Ecuador). Amazotaxia new genus is described from South America for Plesiofornax tetratoma Chassain, creating Amazotaxia tetratoma (Chassain) new combination. Morphology …


Genital Tract Infections, The Vaginal Microbiome And Gestational Age At Birth Among Pregnant Women In South Africa: A Cohort Study Protocol, Ranjana M.S. Gigi, Mandisa M. Mdingi, Hyunsul Jung, Shantelle Claassen-Weitz, Lukas Bütikofer, Jeffrey D. Klausner, Christina A. Muzny, Christopher M. Taylor, Janneke H.H.M. Van De Wijgert, Remco P.H. Peters, Nicola Low Dec 2023

Genital Tract Infections, The Vaginal Microbiome And Gestational Age At Birth Among Pregnant Women In South Africa: A Cohort Study Protocol, Ranjana M.S. Gigi, Mandisa M. Mdingi, Hyunsul Jung, Shantelle Claassen-Weitz, Lukas Bütikofer, Jeffrey D. Klausner, Christina A. Muzny, Christopher M. Taylor, Janneke H.H.M. Van De Wijgert, Remco P.H. Peters, Nicola Low

School of Medicine Faculty Publications

Introduction Preterm birth complications are the most common cause of death in children under 5 years. The presence of multiple microorganisms and genital tract inflammation could be the common mechanism driving early onset of labour. South Africa has high levels of preterm birth, genital tract infections and HIV infection among pregnant women. We plan to investigate associations between the presence of multiple lower genital tract microorganisms in pregnancy and gestational age at birth. Methods and analysis This cohort study enrols around 600 pregnant women at one public healthcare facility in East London, South Africa. Eligible women are ≥18 years and …


Structural Basis For Dna Proofreading, Gina Buchel, Ashok Nayak, Karl Herbine, Azadeh Sarfallah, Viktoriia Sokolova, Angelica Zamudio-Ochoa, Dmitry Temiakov Dec 2023

Structural Basis For Dna Proofreading, Gina Buchel, Ashok Nayak, Karl Herbine, Azadeh Sarfallah, Viktoriia Sokolova, Angelica Zamudio-Ochoa, Dmitry Temiakov

Department of Biochemistry and Molecular Biology Faculty Papers

DNA polymerase (DNAP) can correct errors in DNA during replication by proofreading, a process critical for cell viability. However, the mechanism by which an erroneously incorporated base translocates from the polymerase to the exonuclease site and the corrected DNA terminus returns has remained elusive. Here, we present an ensemble of nine high-resolution structures representing human mitochondrial DNA polymerase Gamma, Polγ, captured during consecutive proofreading steps. The structures reveal key events, including mismatched base recognition, its dissociation from the polymerase site, forward translocation of DNAP, alterations in DNA trajectory, repositioning and refolding of elements for primer separation, DNAP backtracking, and displacement …


Effects Of Mars Global Simulant (Mgs-1) On Growth And Physiology Of Sweet Potato: A Space Model Plant, Karthik Chinnannan, Prapooja Somagattu, Hyndavi Yammanuru, Padma Nimmakayala, Manohar Chakrabarti, Umesh K. Reddy Dec 2023

Effects Of Mars Global Simulant (Mgs-1) On Growth And Physiology Of Sweet Potato: A Space Model Plant, Karthik Chinnannan, Prapooja Somagattu, Hyndavi Yammanuru, Padma Nimmakayala, Manohar Chakrabarti, Umesh K. Reddy

School of Integrative Biological and Chemical Sciences Faculty Publications and Presentations

Growing food autonomously on Mars is challenging due to the Martian soil's low nutrient content and high salinity. Understanding how plants adapt and evaluating their nutritional attributes are pivotal for sustained Mars missions. This research delves into the regeneration, stress tolerance, and dietary metrics of sweet potato (Ipomoea batatas) across different Mars Global Simulant (MGS-1) concentrations (0, 25, 50, and 75%). In our greenhouse experiment, 75% MGS-1 concentration significantly inhibited sweet potato growth, storage root biomass, and chlorophyll content. This concentration also elevated the plant tissues' H2O2, proline, and ascorbic acid levels. Higher MGS-1 exposures (50 and 75%) …


Variables Affecting The Extraction Of Antioxidants In Cold And Hot Brew Coffee: A Review, Brian Yust, Frank Wilkinson, Niny Rao Dec 2023

Variables Affecting The Extraction Of Antioxidants In Cold And Hot Brew Coffee: A Review, Brian Yust, Frank Wilkinson, Niny Rao

College of Life Sciences Faculty Papers

Coffee beans are a readily available, abundant source of antioxidants used worldwide. With the increasing interest in and consumption of coffee beverages globally, research into the production, preparation, and chemical profile of coffee has also increased in recent years. A wide range of variables such as roasting temperature, coffee grind size, brewing temperature, and brewing duration can have a significant impact on the extractable antioxidant content of coffee products. While there is no single standard method for measuring all of the antioxidants found in coffee, multiple methods which introduce the coffee product to a target molecule or reagent can be …


Desmoglein-2 As A Cancer Modulator: Friend Or Foe?, Kay Myo Min, Charlie Ffrench, Barbara Mcclure, Michael Ortiz, Emma Dorward, Michael Samuel, Lisa Ebert, Mỹ Mahoney, Claudine Bonder Dec 2023

Desmoglein-2 As A Cancer Modulator: Friend Or Foe?, Kay Myo Min, Charlie Ffrench, Barbara Mcclure, Michael Ortiz, Emma Dorward, Michael Samuel, Lisa Ebert, Mỹ Mahoney, Claudine Bonder

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

Desmoglein-2 (DSG2) is a calcium-binding single pass transmembrane glycoprotein and a member of the large cadherin family. Until recently, DSG2 was thought to only function as a cell adhesion protein embedded within desmosome junctions designed to enable cells to better tolerate mechanical stress. However, additional roles for DSG2 outside of desmosomes are continuing to emerge, particularly in cancer. Herein, we review the current literature on DSG2 in cancer and detail its impact on biological functions such as cell adhesion, proliferation, migration, invasion, intracellular signaling, extracellular vesicle release and vasculogenic mimicry. An increased understanding of the diverse repertoire of the biological …


Lead In Paint-Contaminated Residential Soils With Varying Physicochemical Properties From Three Large Us Cities: Assessment Of Geochemical Forms And Recommendations On Amendments For Immobilization, Hadeer Saleh, Dibyendu Sarkar, Washington Braida, Steve Larson, Rupali Datta Dec 2023

Lead In Paint-Contaminated Residential Soils With Varying Physicochemical Properties From Three Large Us Cities: Assessment Of Geochemical Forms And Recommendations On Amendments For Immobilization, Hadeer Saleh, Dibyendu Sarkar, Washington Braida, Steve Larson, Rupali Datta

Michigan Tech Publications, Part 2

Lead in yard soils has been recognized as the principal source of excess lead absorption among young children. The hazard imposed by soil lead is dependent on the geochemical forms of lead in soils. Soil properties such as pH, soil organic matter, clay, and carbonate content influence the geochemical forms of lead in soil. This study was conducted to investigate the correlation between soil properties and the geochemical speciation of lead in lead paint-contaminated residential soils from three major US cities. A comprehensive field survey was conducted, involving the collection of soils from ten houses in each of the cities: …


A New Addition To The Trademark Litigator's Tool Kit: A Neuroscientific Index Of Mark Similarity, Mark Bartholomew, Zhihao Zhang, Ming Hsu, Andrew S. Kayser, Femke Van Horen Dec 2023

A New Addition To The Trademark Litigator's Tool Kit: A Neuroscientific Index Of Mark Similarity, Mark Bartholomew, Zhihao Zhang, Ming Hsu, Andrew S. Kayser, Femke Van Horen

Journal Articles

With trademark law always striving to keep abreast of new developments in science and technology, the authors of this article propose an innovative, neuroscience-based approach to answering the time-honored question of whether likelihood of consumer confusion exists in a particular dispute.


Metalloproteomic Investigation Of Hg-Binding Proteins In Renal Tissue Of Rats Exposed To Mercury Chloride, Emerson Carlos De Almeida, Victor Diego Faria, Felipe Dalmazzo Cirinêu, Maria G.A. Santiago, Beatriz Miotto, José C.S. Vieira, Camila Pereira Braga, Jiri Adamec, Ana A.H. Fernandes, Marília A.R. Buzalaf, Pedro De Magalhães Padilha Dec 2023

Metalloproteomic Investigation Of Hg-Binding Proteins In Renal Tissue Of Rats Exposed To Mercury Chloride, Emerson Carlos De Almeida, Victor Diego Faria, Felipe Dalmazzo Cirinêu, Maria G.A. Santiago, Beatriz Miotto, José C.S. Vieira, Camila Pereira Braga, Jiri Adamec, Ana A.H. Fernandes, Marília A.R. Buzalaf, Pedro De Magalhães Padilha

School of Medicine Faculty Publications

Results obtained from rat studies indicate that, even at low concentrations, mercurial species cause harmful effects on the kidneys, by inducing the nephrotic oxidative stress response. In the present work, Hg-associated proteins were identified as possible mercury-exposure biomarkers in rat kidneys exposed to low mercury chloride concentrations for 30 days (Hg-30) and 60 days (Hg-60), using metalloproteomic strategies. The renal proteomic profile was fractioned by two-dimensional electrophoresis and the mercury determinations in kidney samples, protein pellets and protein spots were performed using graphite furnace atomic absorption spectrometry. The characterization of Hg-associated protein spots and the analysis of differentially expressed proteins …


27-Hydroxycholesterol And Dna Damage Repair: Implication In Prostate Cancer, Gloria Cecilia Galvan, Nadine Friedrich, Sanjay Das, James Daniels, Sara Pollan, Shweta Dambal, Ryusuke Suzuki, Sergio Sanders, Sungyong You, Hisashi Tanaka, Yeon-Joo Lee, Wei Yuan, Johann De Bono, Irina Vasilevskaya, Karen Knudsen, Michael Freeman, Stephen Freedland Dec 2023

27-Hydroxycholesterol And Dna Damage Repair: Implication In Prostate Cancer, Gloria Cecilia Galvan, Nadine Friedrich, Sanjay Das, James Daniels, Sara Pollan, Shweta Dambal, Ryusuke Suzuki, Sergio Sanders, Sungyong You, Hisashi Tanaka, Yeon-Joo Lee, Wei Yuan, Johann De Bono, Irina Vasilevskaya, Karen Knudsen, Michael Freeman, Stephen Freedland

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

INTRODUCTION: We previously reported that cholesterol homeostasis in prostate cancer (PC) is regulated by 27-hydroxycholesterol (27HC) and that CYP27A1, the enzyme that converts cholesterol to 27HC, is frequently lost in PCs. We observed that restoring the CYP27A1/27HC axis inhibited PC growth. In this study, we investigated the mechanism of 27HC-mediated anti-PC effects.

METHODS: We employed in vitro models and human transcriptomics data to investigate 27HC mechanism of action in PC. LNCaP (AR+) and DU145 (AR-) cells were treated with 27HC or vehicle. Transcriptome profiling was performed using the Affymetrix GeneChip™ microarray system. Differential expression was determined, and gene set enrichment …


Long Oligodeoxynucleotides: Chemical Synthesis, Isolation Via Catching-By-Polymerization, Verification Via Sequencing, And Gene Expression Demonstration, Yipeng Yin, Reed Arneson, Alexander Apostle, Adikari M.D.N. Eriyagama, Komal Chillar, Emily Burke, Martina Jahfetson, Yinan Yuan, Shiyue Fang Dec 2023

Long Oligodeoxynucleotides: Chemical Synthesis, Isolation Via Catching-By-Polymerization, Verification Via Sequencing, And Gene Expression Demonstration, Yipeng Yin, Reed Arneson, Alexander Apostle, Adikari M.D.N. Eriyagama, Komal Chillar, Emily Burke, Martina Jahfetson, Yinan Yuan, Shiyue Fang

Michigan Tech Publications, Part 2

Long oligodeoxynucleotides (ODNs) are segments of DNAs having over one hundred nucleotides (nt). They are typically assembled using enzymatic methods such as PCR and ligation from shorter 20 to 60 nt ODNs produced by automated de novo chemical synthesis. While these methods have made many projects in areas such as synthetic biology and protein engineering possible, they have various drawbacks. For example, they cannot produce genes and genomes with long repeats and have difficulty to produce sequences containing stable secondary structures. Here, we report a direct de novo chemical synthesis of 400 nt ODNs, and their isolation from the complex …


You Can Lead A Horse To Water: Mapping Seasonal Water Resources To Predict Wild Horse Movements On Utah Rangelands, David C. Stoner, Eric Thacker, Linden Greenhalgh, Mark Nelson Dec 2023

You Can Lead A Horse To Water: Mapping Seasonal Water Resources To Predict Wild Horse Movements On Utah Rangelands, David C. Stoner, Eric Thacker, Linden Greenhalgh, Mark Nelson

All Current Publications

All wild horse herd management areas in Utah overlap BLM grazing allotments. Although horses and cattle have similar dietary habits, both species rely heavily on predictable water sources during dry periods. The concentration of wildlife and livestock in mesic areas during droughts can become problems for farmers and livestock producers. We aimed to map the annual distribution of temporary surface water across Utah that cattle, horses, and wildlife could use. Herein we analyzed an 18-year record of satellite imagery to create a statewide map of seasonal surface-water availability for agricultural and wildlife management purposes.


Reference Evapotranspiration Estimation Using Genetic Algorithm-Optimized Machine Learning Models And Standardized Penman–Monteith Equation In A Highly Advective Environment, Shafik Kiraga, R. Troy Peters, Behnaz Molaei, Steven R. Evett, Gary Marek Dec 2023

Reference Evapotranspiration Estimation Using Genetic Algorithm-Optimized Machine Learning Models And Standardized Penman–Monteith Equation In A Highly Advective Environment, Shafik Kiraga, R. Troy Peters, Behnaz Molaei, Steven R. Evett, Gary Marek

Agricultural and Environmental Sciences Faculty Research

Accurate estimation of reference evapotranspiration (ETr) is important for irrigation planning, water resource management, and preserving agricultural and forest habitats. The widely used Penman–Monteith equation (ASCE-PM) estimates ETr across various timescales using ground weather station data. However, discrepancies persist between estimated ETr and measured ETr obtained from weighing lysimeters (ETr-lys), particularly in advective environments. This study assessed different machine learning (ML) models in comparison to ASCE-PM for ETr estimation in highly advective conditions. Various variable combinations, representing both radiation and aerodynamic components, were organized for evaluation. Eleven datasets (DT) were created for the daily timescale, while seven were established for …


One-Participant 401(K): Saving For Retirement And Reducing Taxes, Jessica Groskopf, Cory Walters, Doug Nelson Dec 2023

One-Participant 401(K): Saving For Retirement And Reducing Taxes, Jessica Groskopf, Cory Walters, Doug Nelson

Center for Agricultural Profitability

The adage that farmers and ranchers are often asset-rich and cash poor does indeed appear to be true! According to the United States Department of Agriculture (USDA), just forty percent of farmers contribute to a retirement plan. And because many farmers and ranchers invest profits in their business rather than take a high salary, they show little income on their tax returns. This means they will have paid less into Social Security and their benefits will be lower when they retire. This can create cash flow challenges, especially for an older generation of farm and ranch families wanting to bring …


One-Participant 401(K) As A Tool For Farmers And Ranchers, Jessica Groskopf, Cory Walters, Doug Nelson Dec 2023

One-Participant 401(K) As A Tool For Farmers And Ranchers, Jessica Groskopf, Cory Walters, Doug Nelson

Center for Agricultural Profitability

According to USDA, only 40% of farm households participate in some type of retirement account. For self-employed farmers and ranchers without full-time employees, the opportunity to invest in a One-Participant 401(k) plan is a way to (1) save money for retirement, (2) reduce taxable income, and (3) provide the potential option to borrow from the plan. This series of articles will review these features.

What is a One-Participant 401(k)?

The One-Participant 401(k)is a qualified retirement plan designed specifically for business owners with no full-time employees other than the business owners and their spouses. Visit with your financial planner and tax …


The Nurse's Lived Experience Of Transferring Nutrition Knowledge To Patients: A Descriptive Phenomenology, Kendrah Lynne Cunningham Dec 2023

The Nurse's Lived Experience Of Transferring Nutrition Knowledge To Patients: A Descriptive Phenomenology, Kendrah Lynne Cunningham

Doctoral Dissertations and Projects

This descriptive phenomenological qualitative study aimed to gain insight into the nurse’s lived experience of transferring nutrition knowledge to patients. Nurses play an important role in assisting the patient to become more proficient in health and nutrition literacy to make proper choices related to their nutrition. It is necessary to evaluate the lived experience of the nurse in transferring nutrition knowledge to patients. There is a gap in the literature on nurses’ experience in providing nutrition knowledge to their patients. This descriptive phenomenological qualitative study examines the lived experience of nurses in transferring nutrition education to their patients. This was …


Synthesis And In‑Vitro Anti‑Proliferative With Antimicrobial Activity Of New Coumarin Containing Heterocycles Hybrids, Elbadawy A. Kamoun, Elbadawy A. Kamoun Dec 2023

Synthesis And In‑Vitro Anti‑Proliferative With Antimicrobial Activity Of New Coumarin Containing Heterocycles Hybrids, Elbadawy A. Kamoun, Elbadawy A. Kamoun

Nanotechnology Research Centre

series of new coumarin-N-heterocyclic hybrids, coumarin-quinolines 7a–e, coumarin-acridines 10b,c and coumarin-neocryptolepines 13b,c were synthesized and evaluated for their anticancer and antimicrobial activities. The structures of all synthesized hybrids were confirmed by FT-IR, 1HNMR, 13C-NMR, and MS spectrometry. The anti-proliferative activity of hybrids 7a–e, 10c and 13c were bio-evaluated using MTT-assay against colon (CaCo-2), lung (A549), breast (MDA-MB-231), and hepatocellular carcinoma (HepG-2) human cancer cell lines using doxorubicin as a reference drug. The results demonstrated that, all hybrids displayed moderate to good anti-proliferative activity against the cell lines. The most active hybrids were 7a–d and 10c against CaCo-2 cancer cell line …


The Effects Of Sex Hormones On The Size Of Intestinal Lipoproteins, Andromeda M. Nauli, Ann Phan, Patrick Tso, Surya M. Nauli Dec 2023

The Effects Of Sex Hormones On The Size Of Intestinal Lipoproteins, Andromeda M. Nauli, Ann Phan, Patrick Tso, Surya M. Nauli

Pharmacy Faculty Articles and Research

Larger intestinal lipoproteins are more likely to be retained longer in the intestinal wall, allowing more time for their fat to be hydrolyzed and subsequently taken up by the abdominal viscera. Since men generally accumulate more abdominal visceral fat than women, we sought to determine if males produce larger intestinal lipoproteins compared to females. Using the conscious lymph fistula mouse model, we discovered that the male mice indeed produced larger intestinal lipoproteins than the female mice when they were intraduodenally infused with lipid emulsion. We then employed our differentiated Caco-2 cell model with semipermeable membrane system to determine the effects …


Needle Biopsy Accelerates Pro-Metastatic Changes And Systemic Dissemination In Breast Cancer: Implications For Mortality By Surgery Delay, Hiroyasu Kameyama, Priya Dondapati, Reese Simmons, Macall Leslie, John Langenheim, Yunguang Sun, Misung Yi, Aubrey Rottschaefer, Rashmi Pathak, Shreya Nuguri, Kar-Ming Fung, Shirng-Wern Tsaih, Inna Chervoneva, Hallgeir Rui, Takemi Tanaka Dec 2023

Needle Biopsy Accelerates Pro-Metastatic Changes And Systemic Dissemination In Breast Cancer: Implications For Mortality By Surgery Delay, Hiroyasu Kameyama, Priya Dondapati, Reese Simmons, Macall Leslie, John Langenheim, Yunguang Sun, Misung Yi, Aubrey Rottschaefer, Rashmi Pathak, Shreya Nuguri, Kar-Ming Fung, Shirng-Wern Tsaih, Inna Chervoneva, Hallgeir Rui, Takemi Tanaka

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

ncreased breast cancer (BC) mortality risk posed by delayed surgical resection of tumor after diagnosis is a growing concern, yet the underlying mechanisms remain unknown. Our cohort analyses of early-stage BC patients reveal the emergence of a significantly rising mortality risk when the biopsy-to-surgery interval was extended beyond 53 days. Additionally, histology of post-biopsy tumors shows prolonged retention of a metastasis-permissive wound stroma dominated by M2-like macrophages capable of promoting cancer cell epithelial-to-mesenchymal transition and angiogenesis. We show that needle biopsy promotes systemic dissemination of cancer cells through a mechanism of sustained activation of the COX-2/PGE2/EP2 feedforward loop, …


Colored Sweet Bell And Tapered Pepper Cultivar Evaluation For High Tunnel Production In West-Central Indiana, 2023, Petrus Langenhoven, Dennis Gustavo Toc Mo Dec 2023

Colored Sweet Bell And Tapered Pepper Cultivar Evaluation For High Tunnel Production In West-Central Indiana, 2023, Petrus Langenhoven, Dennis Gustavo Toc Mo

Midwest Vegetable Trial Reports

Colored sweet bell-shaped and tapered pepper is a summer crop that is grown by many small and medium-sized farming operations in Indiana. Growers can choose to grow peppers out in the field or plant them under a protective structure. Sweet peppers, in particular, benefit from the unique growing environment created by a high tunnel. Planting of peppers can start at least 2-4 weeks earlier in the spring, and production can continue into the fall until the first hard freeze. Pepper variety performance data for Indiana is not readily available. We are working hard to change that. To date, we have …


Physically And Chemically‑Crosslinked L‑Arginine‑Loaded Polyvinyl Alcohol‑ Hyaluronic Acid‑ Cellulose Nanocrystals Hydrogel Membranes For Wound Healing: Influence Of Crosslinking Methods On Biological Performance Of Membranes In‑Vitro, Elbadawy A. Kamoun, Elbadawy A. Kamoun Dec 2023

Physically And Chemically‑Crosslinked L‑Arginine‑Loaded Polyvinyl Alcohol‑ Hyaluronic Acid‑ Cellulose Nanocrystals Hydrogel Membranes For Wound Healing: Influence Of Crosslinking Methods On Biological Performance Of Membranes In‑Vitro, Elbadawy A. Kamoun, Elbadawy A. Kamoun

Nanotechnology Research Centre

Two types of polyvinyl alcohol-blended hyaluronic acid and cellulose nanocrystals (PVA/HA/CNCs) hydrogels membranes were developed as delivery agents for L-arginine using chemical/and/physical crosslinking methods for exploring biological performance of the obtained membranes. Chemically and physically/crosslinked membranes were fabricated by solution-casting and freeze-thawing techniques, respectively. The characterization of the crosslinked PVA/HA/CNCs/Larginine membranes were conducted by SEM, FTIR, TGA, swelling ratio, and in—vitro L-arginine release. Furthermore, applying two types of crosslinked membranes in vitro were investigated in terms of cytotoxicity, hemocompatibility, and cell adhesion affinity of HFB-4 cell line. Chemical and physical-crosslinked membranes were verified through the esterification and hydrogen bonds interactions, …


Treatment Response Of Gingival Squamous-Cell Carcinoma To Palliative Intent Immunotherapy, Natalia Trehan, Angelina Debbas, Mykaihla Sternick, Jennifer Johnson, James Gates Dec 2023

Treatment Response Of Gingival Squamous-Cell Carcinoma To Palliative Intent Immunotherapy, Natalia Trehan, Angelina Debbas, Mykaihla Sternick, Jennifer Johnson, James Gates

Department of Medical Oncology Faculty Papers

The use of PD-1 immune checkpoint inhibitor medications has become a common practice in the treatment of recurrent and metastatic head and neck squamous-cell carcinomas. Success in this setting has led to the investigation of their efficacy in locally advanced cases as a part of first-line therapy. In this report, we detail the treatment response to palliative intent immunotherapy of three geriatric patients with mandibular gingival squamous-cell carcinoma who decided against surgical intervention. Patient #1 was treated with pembrolizumab, a PD-1 inhibitor, and displayed complete clinical and radiologic response of the gingival mass after three months of treatment, which is …


Nucleus Accumbens Core Single Cell Ensembles Bidirectionally Respond To Experienced Versus Observed Aversive Events, Oyku Dinckol, Noah Harris Wenger, Jennifer E Zachry, Munir Gunes Kutlu Dec 2023

Nucleus Accumbens Core Single Cell Ensembles Bidirectionally Respond To Experienced Versus Observed Aversive Events, Oyku Dinckol, Noah Harris Wenger, Jennifer E Zachry, Munir Gunes Kutlu

Rowan-Virtua School of Osteopathic Medicine Faculty Scholarship

Fear learning is a critical feature of survival skills among mammals. In rodents, fear learning manifests itself through direct experience of the aversive event or social transmission of aversive stimuli such as observing and acting on conspecifics' distress. The neuronal network underlying the social transmission of information largely overlaps with the brain regions that mediate behavioral responses to aversive and rewarding stimuli. In this study, we recorded single cell activity patterns of nucleus accumbens (NAc) core neurons using in vivo optical imaging of calcium transients via miniature scopes. This cutting-edge imaging methodology not only allows us to record activity patterns …


Understanding Cut Flower Consumers, Makaylie Langford, Kynda R. Curtis, Melanie Stock Dec 2023

Understanding Cut Flower Consumers, Makaylie Langford, Kynda R. Curtis, Melanie Stock

All Current Publications

This fact sheet provides information on cut flower consumers, such as floral preferences and consumer demographics and psychographics. It discusses possible ways to enhance perceived value and interest in cut flowers.


Protocol For An Agent-Based Model Of Recombination In Bacteria Playing A Public Goods Game, Isaiah Paolo A. Lee, Omar T. Eldakar, J. Peter Gogarten, Cheryl P. Andam Dec 2023

Protocol For An Agent-Based Model Of Recombination In Bacteria Playing A Public Goods Game, Isaiah Paolo A. Lee, Omar T. Eldakar, J. Peter Gogarten, Cheryl P. Andam

Biology Faculty Articles

Agent-based models are composed of individual agents coded for traits, such as cooperation and cheating, that interact in a virtual world based on defined rules. Here, we describe the use of an agent-based model of homologous recombination in bacteria playing a public goods game. We describe steps for software installation, setting model parameters, running and testing models, and visualization and statistical analysis. This protocol is useful in analyses of horizontal gene transfer, bacterial sociobiology, and game theory.

For complete details on the use and execution of this protocol, please refer to Lee et al.1


Mitochondrial Metabolism In Blood More Reliably Predicts Whole-Animal Energy Needs Compared To Other Tissues, Stefania Casagrande, Maciej Dzialo, Lisa Trost, Kasja Malkoc, Edyta T. Sadowska, Michaela Hau, Barbara J. Pierce, Scott R. Mcwilliams, Ulf Bauchinger Dec 2023

Mitochondrial Metabolism In Blood More Reliably Predicts Whole-Animal Energy Needs Compared To Other Tissues, Stefania Casagrande, Maciej Dzialo, Lisa Trost, Kasja Malkoc, Edyta T. Sadowska, Michaela Hau, Barbara J. Pierce, Scott R. Mcwilliams, Ulf Bauchinger

Biology Faculty Publications

Understanding energy metabolism in free-ranging animals is crucial for ecological studies. In birds, red blood cells (RBCs) offer a minimally invasive method to estimate metabolic rate (MR). In this study with European starlings Sturnus vulgaris, we examined how RBC oxygen consumption relates to oxygen use in key tissues (brain, liver, heart, and pectoral muscle) and versus the whole-organism measured at basal levels. The pectoral muscle accounted for 34-42% of organismal MR, while the heart and liver, despite their high mass-specific metabolic rate, each contributed 2.5-3.0% to organismal MR. Despite its low contribution to organismal MR (0.03-0.04%), RBC MR best predicted …