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Full-Text Articles in Life Sciences
Assessment Of A Metaviromic Dataset Generated From Nearshore Lake Michigan, Siobhan C. Watkins, Neil Kuehnle, C Anthony Ruggeri, Kema Malki, Katherine Bruder, Jinan Elayyan, Kristina Damisch, Naushin Vahora, Paul O'Malley, Brianne Ruggles-Sage, Zachary Romer, Catherine Putonti
Assessment Of A Metaviromic Dataset Generated From Nearshore Lake Michigan, Siobhan C. Watkins, Neil Kuehnle, C Anthony Ruggeri, Kema Malki, Katherine Bruder, Jinan Elayyan, Kristina Damisch, Naushin Vahora, Paul O'Malley, Brianne Ruggles-Sage, Zachary Romer, Catherine Putonti
Catherine Putonti
Bacteriophages are powerful ecosystem engineers. They drive bacterial mortality rates and genetic diversity, and affect microbially mediated biogeochemical processes on a global scale. This has been demonstrated in marine environments; however, phage communities have been less studied in freshwaters, despite representing a potentially more diverse environment. Lake Michigan is one of the largest bodies of freshwater on the planet, yet to date the diversity of its phages has yet to be examined. Here, we present a composite survey of viral ecology in the nearshore waters of Lake Michigan. Sequence analysis was performed using a web server previously used to analyse …
Does Baylisascaris Procyonis Phylogeny Correlate With That Of The Raccoon (Procyon Lotor), Matthew E. Ingle, Stephen G. Dunbar, Jaynee L. Bartsch, Kyle J. Culbertson, Taylor A. Fulton, Katherine R. Guffey, Aubrey J. Juris, Ashlie N. Nolan, Daniel P. Nordquist, Carrie E. Rowlands, Joshua A. Sitler
Does Baylisascaris Procyonis Phylogeny Correlate With That Of The Raccoon (Procyon Lotor), Matthew E. Ingle, Stephen G. Dunbar, Jaynee L. Bartsch, Kyle J. Culbertson, Taylor A. Fulton, Katherine R. Guffey, Aubrey J. Juris, Ashlie N. Nolan, Daniel P. Nordquist, Carrie E. Rowlands, Joshua A. Sitler
Stephen Dunbar, PhD
Baylisacaris procyonis, commonly known as the raccoon roundworm, is a parasite that inhabits the small intestine of the North American raccoon (Procyon lotor). Although humans do not typically become the definitive host, humans can become infected through handling soil containing eggs. B. procyonis can induce serious health complications in cases of human infection, including degenerative retinal and behavioral changes, coma, and even death. High prevalence of B. procyonis in a raccoon population increases the probability of transference to human hosts. In our study, we analyzed the genetic structure of B. procyonis harvested from raccoons of southwestern Ohio, and compared this …
Does Baylisascaris Procyonis Impact Raccoon (Procyon Lotor) Genetics?, Matthew E. Ingle, Stephen G. Dunbar, Jaynee L. Bartsch, Kyle J. Culbertson, Taylor A. Fulton, Katherine R. Guffey, Aubrey J. Juris, Ashlie N. Nolan, Daniel P. Nordquist, Carrie E. Rowlands, Joshua A. Sitler
Does Baylisascaris Procyonis Impact Raccoon (Procyon Lotor) Genetics?, Matthew E. Ingle, Stephen G. Dunbar, Jaynee L. Bartsch, Kyle J. Culbertson, Taylor A. Fulton, Katherine R. Guffey, Aubrey J. Juris, Ashlie N. Nolan, Daniel P. Nordquist, Carrie E. Rowlands, Joshua A. Sitler
Stephen Dunbar, PhD
Raccoons (Procyon lotor) are the final host for raccoon roundworms (Baylisascaris procyonis). Raccoon roundworm is the leading cause of a dangerous neurological disease, known as larva migrans encephalopathy. Phylogenetic trees illustrate co-evolutionary events between species living in a symbiotic relationship with each other. Throughout the coevolution of host and parasite, many aspects of a population affect the way the members interact with one another and with symbiotic species. In order to evaluate the relationship between host and parasite in regards to diet, we isolated DNA from intestinal wall tissue, amplified a portion of exon 2 from MHC II, and sent …
Crispr-Cas9 Nuclear Dynamics And Target Recognition In Living Cells, Hanhui Ma, Li-Chun Tu, Ardalan Naseri, Maximiliaan Huisman, Shaojie Zhang, David Grünwald, Thoru Pederson
Crispr-Cas9 Nuclear Dynamics And Target Recognition In Living Cells, Hanhui Ma, Li-Chun Tu, Ardalan Naseri, Maximiliaan Huisman, Shaojie Zhang, David Grünwald, Thoru Pederson
David Grünwald
The bacterial CRISPR-Cas9 system has been repurposed for genome engineering, transcription modulation, and chromosome imaging in eukaryotic cells. However, the nuclear dynamics of clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) guide RNAs and target interrogation are not well defined in living cells. Here, we deployed a dual-color CRISPR system to directly measure the stability of both Cas9 and guide RNA. We found that Cas9 is essential for guide RNA stability and that the nuclear Cas9-guide RNA complex levels limit the targeting efficiency. Fluorescence recovery after photobleaching measurements revealed that single mismatches in the guide RNA seed sequence …