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Consequences Of Recruitmentdecisions And Heterogeneity On Age-Specific Breeding Success In A Long-Lived Seabird, L. M. Aubry, David N. Koons, J. Y. Monnat, E. Cam
Consequences Of Recruitmentdecisions And Heterogeneity On Age-Specific Breeding Success In A Long-Lived Seabird, L. M. Aubry, David N. Koons, J. Y. Monnat, E. Cam
David N. Koons
An individual’s age at first reproduction and investment in successive reproductive attempts are involved in mechanisms that can impede somatic repair, resulting in a decline in reproductive abilities with age (reproductive senescence). We used long-term data from the Black-legged Kittiwake, a long-lived seabird, to address the relationship between recruitment age, age-specific breeding success (BS), and reproductive senescence, while accounting for breeding experience and temporal variation in BS. We first detected late-life improvement in BS across all recruitment groups, which we recognized as ‘‘within-generation selection’’ or the selective disappearance of ‘‘frail’’ phenotypes. When such heterogeneity was accurately accounted for, we showed …
Quaternary Dynamics And Plasticity Underlie Small Heat Shock Protein Chaperone Function, Florian Stengel, Andrew J. Baldwin, Alexander J. Painter, Nomalie Jaya, Eman Basha, Lewis E. Kay, Elizabeth Vierling, Carol V. Robinson, Justin L. P. Benesch
Quaternary Dynamics And Plasticity Underlie Small Heat Shock Protein Chaperone Function, Florian Stengel, Andrew J. Baldwin, Alexander J. Painter, Nomalie Jaya, Eman Basha, Lewis E. Kay, Elizabeth Vierling, Carol V. Robinson, Justin L. P. Benesch
Elizabeth Vierling
Small Heat Shock Proteins (sHSPs) are a diverse family of molecular chaperones that prevent protein aggregation by binding clients destabilized during cellular stress. Here we probe the architecture and dynamics of complexes formed between an oligomeric sHSP and client by employing unique mass spectrometry strategies. We observe over 300 different stoichiometries of interaction, demonstrating that an ensemble of structures underlies the protection these chaperones confer to unfolding clients. This astonishing heterogeneity not only makes the system quite distinct in behavior to ATP-dependent chaperones, but also renders it intractable by conventional structural biology approaches. We find that thermally regulated quaternary dynamics …