Life Sciences Commons^™

Comparison Of Preparative Techniques For Scanning Electron Microscopy Examination Of Soybean Seed Coats In Sectional View, Daniel M. Baker, Harry C, Minor, Merton F. Brown

Scanning Electron Microscopy

Various scanning electron microscopy preparative techniques have been used by researchers to examine sectional views of dry, mature soybean (Glycine~ CL.) Merr.) seed coats. Such previously employed techniques were utilized In our preliminary investigations of seed coat structure, but often yielded unacceptable preservation. Consequently, eight preparative techniques were evaluated In an effort to define conditions required to obtain consistently high quality preservation of soybean seed coats In sectional view. Of the eight procedures tested with the cultivar Williams 82, razor sections and mechanical fractures of dry seed coats yielded the poorest definition of anatomical features. Samples soaked In water prior …

Long Term Effects Of Radiation And Combined Modalities On Mouse Lung, David P. Penney, Paul Van Houtte, Dietmar W. Siemann, Wayne A. Rosenkrans Jr., Philip Rubin, Robert A. Cooper Jr.

Scanning Electron Microscopy

The lung appears to be the major dose-limiting organ in radiation of the thorax. Early responses (<1 week) involve the type II pneumocyte and increased surfactant biosynthesis and secretion. Later changes, which appear to be related to the surfactant response, lead to classical radiation pneumonitis, which is often fatal. Animals which survive radiation pneumonitis develop progressive fibrosis, a late-appearing response, which reduces compliance and available air space, and is usually fatal. This study centers on the fine structural changes in the lungs of LAF₁ mice, 63 weeks following various radiation exposures (5-13 Gy). Doses which are subthreshold in evoking surfactant and pneumonitic responses precipitate fibrosis and atelectasis by 63 weeks, and involve type II pneumocyte sloughing and degeneration. Of the two major deterrents to lung irradiation (pneumonitis and fibrosis), these results suggest that fibrosis always follows pneumonitis, but pneumonitis is not a necessary preliminary step to fibrosis. Bleomycin elicits several morphological alterations characteristic of radiation, and, when combined with the latter, appears to exacerbate radiation effects.

Characterization Of Small Metallic Clusters By Electron Energy Loss Spectroscopy, M. Acheche, C. Colliex, P. Trebbia

Scanning Electron Microscopy

Small spherical tin and gallium clusters (diameters in the range 2 to 80 nm), prepared with a liquid metal ion source, were analysed in a scanning transmission electron microscope (STEM) by electron energy loss spectroscopy (EELS). Both volume and surface plasmon excitations were investigated and their dependence on the cluster size interpreted by classical and quantum mechanical models. A blue shift of the volume plasmon energy with decreasing radius R of the cluster was clearly detected. The full width at half maximum (FWHM) of the peak, related to the damping of the volume plasmon excitation, showed a dependence in 1/R. …

Research Note: Getting Graphic: Computer Graphics In Biochemistry, Wilmon B. Chipman

Bridgewater Review

No abstract provided.

Front Matter

Henry Ford Hospital Medical Journal

No abstract provided.

Intraocular Lens Implant Image Quality: The Optico-Retinal Interface, David D. Bogorad

Henry Ford Hospital Medical Journal

Improvements in intraocular lens implants during the past ten years have revolutionized cataract surgery. Both anterior and posterior chamber lenses are currently in wide use, but the optical superiority of the posterior chamber location has yet to be demonstrated. To do so, a more detailed analysis is required of visual function than is clinically routine. Also required is appreciation that the retina is an integral part of the eye's optical system because of its directional characteristics, which are known as the Stiles-Crawford effect. The optical quality of the pseudophakic eye is affected by the optics of the implant, its centration, …

Immunocytologic Methods In The Diagnosis Of Orbital Tumors, David M. Reifler, Sudha R. Kini, John S. Kennerdell, Andrew Dekker, Leslie J. Fisher

Henry Ford Hospital Medical Journal

The pathologic diagnosis was supported or confirmed in three out of four cases that had an adequate cytologic specimen. The results demonstrate that adjunctive immunocytologic techniques can be used in combination with fine-needle aspiration for a variety of orbital tumors. The pathologic diagnosis was supported or confirmed in three out of four cases that had an adequate cytologic specimen. The results demonstrate that adjunctive immunocytologic techniques can be used in combination with fine-needle aspiration for a variety of orbital tumors.

Some Unusual Foreign Bodies In The Anterior Segment Of The Eye And The Orbit, Saul Sugar

Henry Ford Hospital Medical Journal

No abstract provided.

Malnutrition And Length Of Stay - A Relationship?, Marvin D. Anderson, Gladys Collins, Gloria Davis, Brack A. Bivins

Henry Ford Hospital Medical Journal

The admission nutrition status of 135 consecutive general medical patients admitted to a single nursing unit was evaluated using a simple questionnaire and available laboratory studies. A statistical analysis of the data obtained indicates that prolonged length of stay may be related to admission nutritional status.

Childhood Lead Poisoning, Raj P. Warrier, Jan Waisanen, Sam R. Kottamasu, Sayeed Sultana, K. Ratnakar Kini, William L. Kestenberg, John Strauthers, Craig Foreback

Henry Ford Hospital Medical Journal

Although lead encephalopathy is rare, lead intoxication continues to be a significant problem in Detroit. Neuropsychological effects may occur even at low levels of lead. Since treatment of lead poisoning consists of a painful series of injections, it is imperative that more attention be given to the prevention of this disease. To confirm the magnitude of the problems, our experience with lead poisoning during a three-year period at Henry Ford Hospital's Pediatric Department is reviewed.

Primary Hyperparathyroidism: Changing Patterns In Presentation And Treatment Decisions In The Eighties, D. Sudhaker Rao

Henry Ford Hospital Medical Journal

No abstract provided.

Dermatomyositis Complicated By Thrombotic Thrombocytopenic Purpura, Maria A. Sawdyk, Jeffrey Jundt

Henry Ford Hospital Medical Journal

A 65-year-old woman who had a documented history of dermatomyositis was hospitalized for evaluation of a syncopal attack. During the course of her stay, she experienced progressive neurologic dysfunction, hemolytic anemia, and thrombocytopenia. A clinical diagnosis of thrombotic thrombocytopenia purpura (TTP) was made, and therapy was initiated with glucocorticoids, plasmapheresis, plasma infusion, and antiplatelet medication. The pathogenesis of TTP has not been cleady elucidated. However, reports in the literature have postulated immune damage of the endothelium with demonstration of IgM and complement deposits as the origin of the condition. Further, there are reported cases of TTP associated with collagen disorders …

Clinical Trials: Ii. Randomization And Sample Size, Barbara Tilley, Anthony Schork

Henry Ford Hospital Medical Journal

This second in a series of articles focuses on clinical trials. Information is provided on determining sample size and on methods of randomization including simple and single- and double-consent randomization, and blocked, stratified, and adaptive procedures to randomize patients to a study group.

Hereditary Sclerocornea, David Barsky, Steven P. Dunn

Henry Ford Hospital Medical Journal

A rare case of total hereditary sclerocornea is presented along with clinical history, histopathology, and a review of the possible pathogenesis and current literature on the subject. It is quite possible that imperfect cleavage of the anterior chamber angle may be the predisposing factor in scleralization of the cornea due to a failure of neural crest cell differentiation. If surgical intervention to correct for the corneal opacification is indicated, corneal transplantation should be performed as soon as possible after birth, similar to the indications for removal of dense congenital cataracts to avoid deprivation amblyopia.

Back Matter

Henry Ford Hospital Medical Journal

No abstract provided.

Glaucoma Filtration Surgery: A Personal Review, Saul Sugar

Henry Ford Hospital Medical Journal

No abstract provided.

Orbital Metastasis With Enophthalmos: A Review Of The Literature, David M. Reifler

Henry Ford Hospital Medical Journal

Orbital metastasis is associated with enophthalmos in up to one third of all cases. After analyzing the clinical and pathologic features In 25 reported cases of enophthalmic orbital metastasis, the condition was found to almost exclusively affect females, usually in the 60 to 70 age group. The right orbit was the most commonly affected. The primary lesion was located in the breast in 19 (76%) cases and in the abdomen in 6 (24%) cases. In one third of the cases, the manifestations of the orbital metastasis were recognized before diagnosing the primary lesion. The most common presenting features were diplopia …

The Pseudo-Leukocyte-Specific/Nuclear Membrane Antinuclear Antibody Pattern: A Puzzle, Thomas K. Burnham

Henry Ford Hospital Medical Journal

We report an antinuclear antibody (ANA) pattern—the pseudo-leukocyte-specific/nuclear membrane (PLS/NM) ANA pattern—that is puzzling because it usually reacts with only a few cells on human spleen imprints but with most epidermal and numerous dermal nuclei of normal human skin sections. The designation "pseudo-leukocyte-specific" was chosen because it is generally seen in only a few nuclei on human spleen imprints, thereby resembling the true leukocyte-specific ANA pattern. A few small round nuclei fluoresce homogeneously, surrounded by a bright wall, suggesting nuclear membrane (NM) fluorescence. The term "nuclear membrane" was added to the name because the NM appearance is even more prominent …

Editorial: Corneal Stability With Rigid Gas Permeable Lens Refitting, Robert J. Crossen

Henry Ford Hospital Medical Journal

No abstract provided.

Evaluation Of Peutz-Jeghers Syndrome In A Family, Kayhan Ozkan

Henry Ford Hospital Medical Journal

Five cases of Peutz-Jeghers syndrome in one family have been studied, and a review of the history and features of the syndrome are presented. This syndrome was detected in four of six siblings and in the father. Three family members underwent surgery after small-bowel obstruction was diagnosed. A gastric polyp was removed from a fourth family member. Rectal bleeding dominated the clinical picture in two patients. Colonic polyps were also found in the three patients who had small-bowel polyps. One of the four affected siblings had mucocutaneous pigmentation, but no evidence of intestinal polyps was detected. The five affected family …

Opportunities For Electron Microscopy In Space Radiation Biology, J. T. Lett

Scanning Electron Microscopy

Densely ionizing, particulate radiations in outer space are likely to cause to mammalian tissues biological damage that is particularly amenable to examination by the techniques of electron microscopy. This situation arises primarily from the fact that once the density of ionization along the particle track exceeds a certain value, small discrete lesions involving many adjacent cells may be caused in organized tissues. Tissue damage produced by ionization densities below the critical value also afford opportunities for electron microscopic evaluation, as is shown by the damage produced in optic and proximate tissues of the New Zealand white rabbit in terrestrial experiments. …

An Organic Reference Specimen: Polyacrylonitrile Film Deposited On Oxidizable Metals By Electro-Polymerization, C. Boiziau, G. Lécayon

Scanning Electron Microscopy

Surface investigations of organic materials require the provision of reference specimens for calibrating experimental equipment.

Because of their specific properties (purity, reproducibility, well-known molecular structure and orderly inter-molecular structure, electronic properties associating conductive and insulating behaviour, quality of the film-to-metal bound), polyacrylonitrile films obtained through electro-polymerization on oxidizable metal cathodes (Fe, Ni, Cu) may be used as a first type of reference sample.

Experimental results, obtained by various surface investigation techniques are presented, and their validity evaluated.

Louisiana Agriculture Magazine, Fall 1985, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

Cathodoluminescence Study Of Defects In Iii-V Substrates And Structures, M. Cocito, P. Franzosi, G. Salviati, F. Taiariol

Scanning Electron Microscopy

Solid state detector cathodoluminescence studies of semiconducting and semi-insulating GaAs and InP crystals, were performed. The origin of the dislocation contrast in GaAs:Si doped substrates, in the carrier concentration range from 10¹⁶ to 6 · 10¹⁸ cm^-3, were discussed. The image contrast was explained on the basis of the emission efficiency versus carrier concentration curve, obtained in the transmission mode. Single dislocations and dislocation arrangements in addition to growth striations, clusters and precipitate-like microdefects were evidenced. The above mentioned microdefects were detected in GaAs: Te, S and Si doped and InP: Sn doped specimens. Commercial InP:Sn …

A High Efficiency Cathodoluminescence System And Its Application To Optical Materials, A. D. Trigg

Scanning Electron Microscopy

A high collection efficiency spectroscopic cathodoluminescence (CL) system based on an in-line ellipsoidal mirror has been constructed for use on a Cambridge S250 scanning electron microscope (SEM). It can be fitted to or removed from the SEM in about 30 minutes and requires no significant modification of the instrument. It can be used to obtain total CL images, monochromatic CL images or CL spectra with an ultimate spectral resolution of 1 nm.

The system has been applied to the study of doped synthetic quartz crystals, optical fibres and optical fibre preforms, and to yttrium aluminium garnet, bismuth silicon oxide and …

Fundamental Aspects Of Energetic Particle/Solid Interactions, Nicholas Winograd

Scanning Electron Microscopy

The interaction of keV particles with solids has been characterized by the measurement of the angle and energy distribution of sputtered secondary ions and neutrals. The results are compared to classical dynamics calculations of the ion impact event. Examples using secondary ions are given for clean Ni{001}, Cu{001} reacted with O₂, Ni{001} and Ni{7 9 11) reacted with CO, and Ag{111} reacted with benzene. The neutral Rh atoms desorbed from Rh{001} are characterized by multiphoton resonance ionizaton of these atoms after they have left the surface.

The Cathodoluminescence Mode Of The Scanning Electron Microscope: A Powerful Microcharacterization Technique, D. B. Holt, F. M. Saba

Scanning Electron Microscopy

By detecting cathodoluminescence (CL) in a scanning electron microscope (SEM), pan and monochromatic micrographs and CL spectral analyses analogous to x-ray mode point analyses can be obtained. Complete microcharacterization requires alternate examination of both micrographs and spectra. New techniques for near infra-red CL and low-temperatures to produce sharp spectra are of increasing importance. CL emission is due to electron transitions between quantum mechanical states so the radiative defects present can be unambiguously identified at liquid helium temperatures. Strongly luminescent impurities can be detected to below one part in 10⁸. This is 10⁴ times as sensitive as the …

Liquid Metal Sources In Ion Microscopy And Secondary Ion Mass Spectrometry, G. L. R. Mair, T. Mulvey

Scanning Electron Microscopy

This paper reports on state-of-the-art developments in liquid metal sources and some of the finer points of their operating characteristics that are especially relevant to the satisfactory functioning of analytical ion scanning microscopes equipped with secondary ion mass spectrometers. Such effects include unwanted emissions from the source and their exclusion by means of filters and mass separators in the ion-optical column. The design of the ion-optical column is also discussed and some applications of this rapidly advancing form of analytical microscopy are described.

Secondary Ion Mass Spectrometry Of Glasses: Aspects Of Quantification, H. Odelius, A. R. E. Lodding, L. O. Werme, D. E. Clark

Scanning Electron Microscopy

SIMS routines have been developed for the analysis of oxide materials, with applications particularly in element profiling of corrosion layers on glasses after weathering or leaching. The possibilities of quantification and reproducibility have been found critically sensitive to the buildup of charge on the insulating specimens. With control of constant specimen potential, relative sensitivity factors in the positive mass spectrum have been determined for about 20 elements in 10 different alkali-borosilicate glasses. Secondary ion yields were studied as functions of the energy range of ions admitted to the analyzer. At relatively low energies, including the top of the energy distribution, …

A New Approach For Studying Semithin Sections Of Human Pathological Material: Intermicroscopic Correlation Between Light Microscopy And Scanning Electron Microscopy, G. Pasquinelli, C. Scala, G. P. Borsetti, F. Martegani, R. Laschi

Scanning Electron Microscopy

In order to obtain useful and complete information on the study of pathological material, we observed by scanning electron microscopy (SEM) the same semithin sections observed by light microscopy (LM). For this purpose, the specimen must have, at the same time, chromatic and electron dense characteristics.

We thus developed different specimen preparation methods, subjecting the semithin sections to specific polychromatic staining with high atomic number (Z) elements, to monochromatic staining followed by routine contrasting with uranyl acetate and lead citrate, and to specific cytochemical and immunocytochemical procedures.

The specimens were examined in sequence by LM, by SEM equipped with secondary …