Life Sciences Commons^™

Lower Body Gait Variability As A Distinguishing Feature In Humans, Tyler M. Wiles, Seung Kyeom Kim, Nick Stergiou, Aaron D. Likens

UNO Student Research and Creative Activity Fair

Most humans have a fingerprint that is unique and persists throughout life. The same may be true for gait. Each person is unique physiologically and biomechanically, and has experienced a unique progression of life including injuries, habits, hobbies, or lifestyles that all contribute to navigating the world. Those individualized experiences, whether intended or not, are potentially on display in gait. We hypothesized that there are gait characteristics intrinsic and unique to everyone, so that everyone has a unique “gaitprint”, similar to humans possessing unique fingerprints. To test our hypothesis, we recruited thirty healthy young adults between the ages 19-35 as …

Walking With A Load On Wheels: Potential Effects Of Bidirectional Springs On Walking Economy, Hiva Razavi, Philippe Malcolm

UNO Student Research and Creative Activity Fair

Walking with a Load on Wheels: Potential Effects of Bidirectional Springs on Walking Economy

Have you ever noticed how your arms alternatively flex and extend when pushing a heavy shopping cart? Accelerating and decelerating heavy masses, such as a shopping cart, can require much effort. During walking, we alternatively accelerate and decelerate our Center of Mass (COM) in every gait cycle, which can require even more effort if we accelerate and decelerate a load while walking. Theoretically, this effort can be reduced by timing the acceleration and deceleration of the load's mass opposite to the individual's COM. Previous studies have …

Materials And Methods Developed For The Recording And Analysis Of Behavior In The Common Marmoset (Callithrix Jacchus)., Christian Wintle

UNO Student Research and Creative Activity Fair

Materials and methods developed for the recording and analysis of behavior in the common marmoset (Callithrix jacchus).

Christian J. Wintle^a, Jordan B. Hernandez^a,b,c, Dobromir Dotov^d, and Jonathan B. Clayton^a,b,e,f,g

^aDepartment of Biology, University of Nebraska at Omaha, Omaha, NE, USA

^bNebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA

^cDepartment of Genetics, Cell Biology, and Anatomy, University of Nebraska Medical Center, NE, USA

^dDepartment of Biomechanics, University of Nebraska at Omaha, Omaha, NE, USA

^eDepartment of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, …

Inter-Joint Variability And Age-Related Changes In Human Walking, Mehrnoush Haghighatnejad, Tyler Wiles, Seung Kyeom Kim, Aaron Likens, Nicholas Stergiou

UNO Student Research and Creative Activity Fair

Inter-Joint Variability and Age-Related Changes in Human Walking

Mehrnoush Haghighatnejad¹*, Tyler M. Wiles¹, Seung Kyeom Kim¹, Nick Stergiou^1,2, Aaron D. Likens¹

¹Department of Biomechanics, University of Nebraska at Omaha

²Department of Physical Education and Sport Science, Aristotle University, Thessaloniki, Greece

*Corresponding author’s email: mhaghighatnejad@unomaha.edu

Presentation Preference: Poster

Humans routinely perform a variety of activities such as walking, running, driving, and typing, all of which require coordination among different body joints. For instance, walking and running involve rhythmic movements of the arms and legs. The involvement of the upper …

Negative Impact Of Aging On Gait Autocorrelation: A Mixed-Effects Analysis Of Stride Interval Dynamics, Theodore Deligiannis, Tyler M. Wiles, Seung Kyeom Kim, Nikolaos Stergiou, Aaron Likens

UNO Student Research and Creative Activity Fair

NEGATIVE IMPACT OF AGING ON GAIT AUTOCORRELATION: A MIXED-EFFECTS ANALYSIS OF STRIDE INTERVAL DYNAMICS

Theodore A. Deligiannis^1*, Tyler M. Wiles¹, Seung Kyeom Kim¹, Nick Stergiou^1,2, Aaron D. Likens¹

¹Department of Biomechanics, University of Nebraska at Omaha

*Corresponding author’s email: tdeligiannis@unomaha.edu

Presentation preference: Podium

Traditional gait metrics, like speed, cadence, coordination, as well as non-linear measures, appear related to Timed Up and Go (TUG). Timed Up and Go is a clinical measurement related to physical, socioeconomic, and psychological parameters, as well as overall health. TUG can be used as a health …

Human Blood Cell Isolation: The Critical First Step In Our Laboratory’S Immunobiology Experimental Protocals, Victor Rivero

UNO Student Research and Creative Activity Fair

HUMAN BLOOD CELL ISOLATION: THE CRITICAL FIRST STEP IN OUR LABORATORY’S IMMUNOBIOLOGY EXPERIMENTAL PROTOCALS

Victor Rivero1 Paul W. Denton1, vrivero@unomaha.edu

1Department of Biology, University of Nebraska at Omaha, Omaha, NE

The Denton Immunobiology Laboratory focuses on enhancing human natural killer (NK) cell killing capabilities, particularly in the context of combating cancer. NK cells are immune cells that have the ability to kill diseased cells via two mechanisms: direct killing, and antibody-dependent cell-mediated cytotoxicity (ADCC). We recently published our novel approach to testing both methods of killing by using NK cells derived from the same human donor. Our testing approach allows …

Accelerometry-Based Analysis Of Postural Sway In Parkinson's Disease Patients With Levodopa-Induced Dyskinesia, Chandler Brock

UNO Student Research and Creative Activity Fair

Parkinson’s disease (PD) is a progressive neurodegenerative disorder, with patient numbers projected to double to 12 million in the next 20 years. Levodopa-induced dyskinesia (LID) is a major problem associated with the long-term use of levodopa for symptomatic treatment of PD. These involuntary movements can become disabling and may interfere with quality of life. Our prior research showed that PD w/ LID were less stable while standing (i.e., increased postural sway) and had a higher incidence of falls. The aim of this study is to determine if postural sway properties are altered by LID via decomposing the sway signal. We …

Walking Easier By Attaching A Spring-Mass To The Body: A Preliminary Simulation, Hiva Razavi, Philippe Malcolm

UNO Student Research and Creative Activity Fair

When carrying or pushing a load, more force is needed to accelerate and decelerate the additional mass, increasing Ground Reaction Force (GRF). However, this can be reduced by synchronizing the object's movement with the individual's Center of Mass (COM) using antiphase acceleration and deceleration. Past studies have shown this can decrease muscle work and metabolic costs. This study aimed to determine optimal spring parameters to minimize horizontal GRF (GRFh) when pushing a cart by connecting the human to the cart with a spring and damper system.

Three walking conditions were simulated in MATLAB: 1) Normal walking; 2) Walking with a …

Attenuated Skeletal Muscle Metabolism Explains Blunted Reactive Hyperemia After Prolonged Sitting, Cody Anderson, Elizabeth Pekas, Michael Allen, Song-Young Park

UNO Student Research and Creative Activity Fair

Introduction: Although reduced post-occlusive reactive hyperemia (PORH) after prolonged sitting (PS) has been reported as impaired microvascular function, no specific mechanism(s) have been elucidated. One potential mechanism, independent of microvascular function, is that an altered muscle metabolic rate (MMR) may change the magnitude of PORH by modifying the oxygen deficit achieved during cuff-induced arterial occlusions. We speculated that if MMR changes during PS, this may invalidate current inferences about microvascular function during PS. Objective: Therefore, the objective of this study was to examine if peripheral leg MMR changes during PS and to ascertain whether the change in the oxygen deficit …

The Effect Of Exoskeleton Footwear On Joint Angular Motion During Walking In Patients With Peripheral Artery Disease, Zahra Salamifar, Farahnaz Fallahtafti, Iraklis I. Pipinos, Jason M. Johanning, Sara Myers

UNO Student Research and Creative Activity Fair

Gait, Lower Extremity, Peripheral Artery Disease, Joint Angle, Exoskeleton Footwear, Exoskeleton, Assistive Device, Walking.

Histological Evidence For The Therapeutic Effect Of Chitosan Nanofibrous Dressing On Acute Skin Wounds In A Rat Model, Mahboubeh Ghanbari, Sayed Ahmadreza Razian, Sara Cartwright, Yury Salkovskiy, Jason Mactaggart, Mark A. Carlson

UNO Student Research and Creative Activity Fair

Introduction: Large-area skin traumas, such as thermal burns, are among the most severe health issues that decrease patients’ quality of life and burden healthcare systems. The CDC estimates that there are 1.1 million burns requiring medical attention each year, with more than 20,000 cases involving at least 25% of the body surface, resulting in 4,500 deaths. In addition, about 10,000 people die from burn-related infections. A promising solution to alleviate this problem is using wound dressings based on biopolymers with inherent wound healing properties and biodegradability. One of these biopolymers is chitosan, which is derived from arthropod shells and exhibits …

The Effects Of Demographics And Risk Factors On The Morphological Characteristics Of Human Femoropopliteal Arteries, Sayed Ahmadreza Razian, Majid Jadidi, Alexey Kamenskiy

UNO Student Research and Creative Activity Fair

Background: Disease of the lower extremity arteries (Peripheral Arterial Disease, PAD) is associated with high morbidity and mortality. During disease development, the arteries adapt by changing their diameter, wall thickness, and residual deformations, but the effects of demographics and risk factors on this process are not clear.

Methods: Superficial femoral arteries from 736 subjects (505 male, 231 female, 12 to 99 years old, average age 51±17.8 years) and the associated demographic and risk factor variables were used to construct machine learning (ML) regression models that predicted morphological characteristics (diameter, wall thickness, and longitudinal opening angle resulting from the …

Effect Of Dual Tasking And Levodopa-Induced Dyskinesia On Postural Sway In People With Parkinson's Disease, Joseph Ayotunde Aderonmu, Carolin Curtze

UNO Student Research and Creative Activity Fair

Parkinson's disease (PD) is a neurodegenerative disorder that results in motor impairments such as gait and balance deficits. Levodopa is one of the most effective drugs in treating the slowness of movement in individuals with PD. However, the long-term use of levodopa in treating PD often causes undesirable involuntary and uncontrollable movements, known as levodopa-induced dyskinesia (LID). LID is a known cause of increased postural sway. Yet, the relative contribution of the body segments often affected by dyskinesia to postural sway is unknown. We aimed to investigate the contribution of different body segments to postural sway in PD for individuals …

Endothelial Cell Nrf2-Ko Attenuates Endothelial Function And Skeletal Muscle Antioxidant Capacity, Elizabeth J. Pekas, Cody P. Anderson, Michael F. Allen, Kollyn B. Jallen, Dong Gun Jin, Andres Benitez-Albiter, Christian D. Sanchez Tato, Lie Gao, Neha Dhyani, Tara L. Rudebush, Irving H. Zucker, Song-Young Park

UNO Student Research and Creative Activity Fair

INTRODUCTION: Endothelial cells line the inner surface of blood vessels and play a major role in modulating blood flow and gas exchange. Endothelial dysfunction is thought to be a contributor to cardiovascular disease development, and it is well-accepted that excessive reactive oxygen species (harmful molecules) likely contribute to endothelial dysfunction. The transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) is considered the master regulator of cellular protection in response to elevated reactive oxygen species. Therefore, Nrf2 may be a potential therapeutic target to protect against endothelial dysfunction. However, the roles of endothelial cell-specific Nrf2 on endothelial function are not …

Impaired Hydrogen Sulfide Protein Expression In Patients With Peripheral Artery Disease, Dong Gun Jin, Kollyn B. Jallen, Liz Pekas, Song-Young Park

UNO Student Research and Creative Activity Fair

INTRODUCTION: Hydrogen sulfide (H₂S) is a gaseous signaling molecule that serves various roles in the vasculature, such as upregulating angiogenesis, vascular smooth muscle relaxation, protecting endothelial function, and regulating redox balance. Despite H₂S’s positive impacts on vascular homeostasis, it is important to note that its actions depend on its concentrations. At high concentrations, H₂S has been reported to increase oxidative stress damage, such as oxidation of cysteine residues and lipid peroxidation. This may indicate that H₂S may act as a ‘double-edged sword’ in the field of vascular physiology. Peripheral artery disease (PAD) …

The Optimal Relationship Between Actuator Stiffness And Actuation Timing For A Passive Ankle Exoskeleton: An Opensim Simulation, Cody Anderson, Hafizur Rahman, Sara Myers

UNO Student Research and Creative Activity Fair

Introduction

The passive ankle exoskeleton developed by Collins et al. (2015) reduced the metabolic cost of walking with an actuation-timing of ~16% of stance [1]; however, other actuation timings have not been extensively investigated. Therefore, the purpose of this study was to determine the optimal relationship between actuator-stiffness and actuation-timing for a passive ankle exoskeleton by using musculoskeletal modeling.

Methods

Kinematics and ground reaction forces were recorded while a healthy-young male walked on overground force-plates, and these data were exported to a musculoskeletal modeling software (OpenSim) for simulation. A passive ankle exoskeleton model was designed and integrated with a default …

Design And Development Of Software With A Graphical User Interface To Display And Convert Multiple Microscopic Histology Images, Sayed Ahmadreza Razian, Majid Jadidi, Alexey Kamenskiy

UNO Student Research and Creative Activity Fair

Histological images are widely used to assess the microscopic anatomy of biological tissues. Recent advancements in image analysis allow the identification of structural features on histological sections that can help advance medical device development, brain and cancer research, drug discovery, vascular mechanobiology, and many other fields. Histological slide scanners create images in SVS and TIFF formats that were designed to archive image blocks and high-resolution textual information. Because these formats were primarily intended for storage, they are often not compatible with conventional image analysis software and require conversion before they can be used in research. We have developed a user-friendly …

Ankle Foot Orthoses Improves Ground Reaction Forces In Patients With Peripheral Artery Disease, Zahra Salamifar, Farah Fallahtafti, Iraklis Pipinos, Jason Johanning, Hafizur Rahman, Mahdi Hassan, Sara Myers

UNO Student Research and Creative Activity Fair

Peripheral artery disease (PAD) is caused by atherosclerotic blockage of the arteries in the legs. Globally, over 236 million people suffer from PAD. Intermittent claudication is the most common PAD symptom that includes muscle pain, cramping, and/or aching induced by physical activities and relieved with rest. PAD impacts gait patterns: specifically, rapid ankle plantar flexion after heel contact, which decreases optimal energy transfer. An ankle-foot orthosis (AFO) is an assistive device that improves gait in patients with neurological conditions. AFOs could help patients with PAD to improve walking kinetics. The purpose of this study was to determine the impact of …

Breaks In Longitudinal Elastic Fibers Of Human Femoropopliteal Arteries, Elham Zamani

UNO Student Research and Creative Activity Fair

Breaks in Longitudinal Elastic Fibers of Human Femoropopliteal Arteries

Elham Zamani1, Majid Jadidi1

1 Department of Biomechanics, University of Nebraska Omaha, Omaha, NE

Introduction: Elastin is a major protein in the body with half-life >50 years. It is thought that elastic fibers are formed before the postnatal period. In the femoropopliteal artery (FPA), the main artery in the leg, longitudinal elastic fibers are present in External Elastic Lamina (EEL). Our team has studied more than 1000 cadaveric human FPA and has noticed that there are big breaks in their longitudinal elastic fibers in some subjects. Our goal in this work …

Efficacy Of The Opensim Simulator As A Tool To Detect Changes In Gait: A Peripheral Artery Disease Model, Cody Anderson, Hafiz Rahman, Irakis Pipinos, Jason Johanning, Sara Myers

UNO Student Research and Creative Activity Fair

INTRODUCTION

Peripheral Artery Disease (PAD) is characterized by atherosclerotic plaques in the leg arteries. A common treatment for PAD is supervised exercise training [1]. However, gait is not fully restored following supervised exercise training (SET). Musculoskeletal simulation may provide insight into how muscle activation changes following SET contributed to persistent gait alterations.

METHODS

Kinematic and kinetic data from overground walking of patients with PAD (n=12), before and after a 6-month exercise intervention, was used as input for musculoskeletal modeling (OpenSim). The subjects in our sample were subdivided based on the location of ischemic muscle pain, as reported on the San …

Development Of A Modular Activity-Specific Upper Limb Prosthesis, Rahul Raj

UNO Student Research and Creative Activity Fair

The use of activity-specific upper-limb prosthesis helps children with upper-limb loss to engage in functional and recreational activities, such as music and sports. The purpose of this study was to develop a modular activity-specific prosthesis and develop a remote-fitting procedure. We evaluated patient satisfaction after using the device for 8 weeks and evaluated anthropometric and range of motion (ROM) measurements of the residual limb after use of the prosthesis. We enrolled 7 children with unilateral trans-radial amputations between 7 and 12 years of age. The modular activity- specific prosthesis was specifically designed for playing musical instruments and sports. A survey …

The Effects Of 3-Weeks Of Aerobic Exercise In Heat On Fitness And Pgc1a In Females, Mark Mcglynn, Robert L. Shute, Brent Ruby, Walter Hailes, Dustin Slivka

UNO Student Research and Creative Activity Fair

The effects of exercise training in the heat are well documented in men. However, the effects of exercise training in the heat in women have not received as much attention. We have previously reported a blunted rise in PGC1α in men after acute aerobic exercise in the heat. Purpose: To determine the impact of three weeks of aerobic exercise training in the heat compared to training in room temperature on thermoregulation, PGC1α mRNA response, and aerobic capacity in women. Methods: Twenty-three untrained college aged females (24±4 yoa, 168±5 cm, and 67.3±11.2 kg) were randomly assigned to 3 weeks of aerobic …

A Systematic Review: Long Range Correlations In Running Gait, Mr. Taylor Wilson

UNO Student Research and Creative Activity Fair

Long range correlations (LRCs) are apparent in human walking and running gait and are indicative of a healthy system. A systematic review (SR) was completed to determine the typical LRC patterns in running. 20 articles were included in the SR. LRCs in running gait tend to be similar to the LRCs in walking gait, with fatigue contributing to a negative effect on the LRCs.

This SR could have implications on training guidelines for coaches and physical therapy methods to increase the LRCs in running gait for healthier functioning. More research is needed to determine how LRCs depend on running surface …

Do Social Networks Impact Perceived Loneliness In Caregivers To Older Adults With Chronic Conditions?, Janelle N. Beadle, Hayley Fouche, Angela Gifford

UNO Student Research and Creative Activity Fair

The abstract is not published at this time to protect intellectual property.

The Effects Of Trichloroethylene On The Germination And Early Development Of Radish, Butternut Squash, Corn, And Soybean, Sarah Jaksich

UNO Student Research and Creative Activity Fair

Trichloroethylene (TCE) is a small molecule used as a metal degreaser and as an extraction solvent for lipids. Historically, TCE has been used to clean missiles and rockets after testing at military sites. However, TCE has decreased in use due to its potential health hazards. TCE is particularly dangerous because it is soluble in water and easily seeps into ground water. As a result, TCE has contaminated groundwater in Mead, Nebraska. The town is near the formal Nebraska Ordnance Plant, a military factory specializing in bomb assembly, ammonium nitrate production, and explosives burning. Soil sampling in Mead indicated TCE levels …

Mitochondrial Targeted Antioxidant Intake Improves Vascular Function And Exercise Tolerance In Peripheral Artery Disease Patients, Liz Pekas, Won-Mok Son, Ronald J. Headid Iii, Tesean Wooden, Song-Young Park

UNO Student Research and Creative Activity Fair

Peripheral artery disease (PAD) is a manifestation of atherosclerosis in the leg arteries, which causes reduced blood flow and leg pain. This may be in part due to excessive mitochondria-produced reactive oxygen species (ROS) and attenuated mitochondrial respiratory function. Mitoquinol mesylate, a mitochondrial-targeted antioxidant, has been shown to scavenge ROS and improve vascular function in older adults and animal models. However, the impacts of mitoquinol on vascular function in PAD patients are unknown. We sought to examine the impacts of mitoquinol intake (80mg) on endothelial function (flow mediated dilation, FMD), resting heart rate (RHR), blood pressure (BP), arterial stiffness (pulse …

Influence Of Cattle Grazing On Milkweed And Other Native Forbs, Karen Klug

UNO Student Research and Creative Activity Fair

One of the most identifiable plants of the Great Plains are milkweed species (Asclepias spp.), which are also the only food source for monarch butterfly caterpillars (Danaus plexippus). Conservation of the milkweed population has taken renewed importance as the population of eastern migratory monarchs has dropped by over 80% from 1996 to 2017, largely because of a loss of over 1.3 billion stems of milkweed. As a result of this monarch population decline, it has been petitioned to protect the monarch butterfly population under the US Endangered Species Act. To increase the population size of the …

How Stair Negotiation Affects Knee Joint Contact Forces In Individuals Post-Total Knee Arthroplasty, Todd Leutzinger

UNO Student Research and Creative Activity Fair

This study investigates how walking up and down stairs affects knee joint contact forces in individuals post-total knee arthroplasty compared to overground level walking and healthy controls.

Developing And Testing Of Low-Cost 3d Printed Prostheses To Restore And Improve Function Of Children With Congenital Or Traumatic Amputations, Rahul Raj, Christopher Copeland, Claudia Cortes Reyes, Kaitlin Fraser, David Salazar

UNO Student Research and Creative Activity Fair

The purpose of this investigation was to provide technical considerations and clinical evidence of the possible benefits and obstacles in the use of upper-limb 3D printed prostheses in paediatric populations. This information is crucial for clinicians interested in exploring the use of 3D printed prostheses for their patients. In the present study, children with congenital digit reductions (n=5); absent digits (one traumatic and four congenital) (n=5) and with upper-limb reductions (n=9). The perceived functionality of upper-limbs before the use of the prosthesis and after 4 months of use. In addition, gross dexterity, anthropometric measurements, active range of motion, and strength …

Determining The Optimal Tension For An Ankle-Foot Assistive Device, Cody Anderson

UNO Student Research and Creative Activity Fair

Introduction

Peripheral artery disease (PAD) is caused by atherosclerosis, a buildup of cholesterol and fatty acid deposits, which blocks blood flow to the arms, legs, and feet. Ankle assistive devices have recently been implemented in various clinical population to improve walking ability. Our group has developed an ankle assistive device to improve walking ability in patients with PAD. This study tested our newly developed assistive device with several tension assistance levels and their effect on metabolic cost.

Methodology

Ten healthy subjects were recruited for this study. A spring was attached to the posterior side of the device parallel to the …