Life Sciences Commons^™

Ethylene Production As An Indicator Of Stress Conditions In Hydroponically-Grown Strawberries, Justin Donald Hogan

Theses, Dissertations and Capstones

With the worldwide phaseout of methyl bromide, the use of hydroponic systems has increased as an economic alternative for the growth of many horticulturally-important crops, including strawberries. In this study, the effect of hydroponics on strawberry plant physiology was examined by first measuring ethylene levels, a plant hormone known to increase due to stressful conditions, and plant growth and yield. Using a gas chromatograph, ethylene was measured from plants which showed that light and temperature have minimal effects, but placement of plants could have an effect on plant growth and yield. Next, the mechanism of ethylene production was examined by …

Ecology, Reproduction And Morphometrics Of The Common Ribbonsnake (Thamnophis Sauritus) And Eastern Gartersnake (Thamnophis Sirtalis) In West Virginia, Noah Mccoard

Theses, Dissertations and Capstones

Two species of gartersnakes, Thamnophis, are found in West Virginia. Thamnophis sauritus , a semi-arboreal and semi-aquatic species, is listed as very rare and imperiled (S2) by the West Virginia Division of Natural Resources (WVDNR) while the other Thamnophis sirtalis sirtalis, a terrestrial species, is secure (S5). During the summer of 2007, I traveled to 53 counties in West Virginia searching for habitat and these two species.Several Thamnophis sirtalis were found in a variety of habitats throughout the state, but only three Thamnophis sauritus were found. Reproductive aspects were compared between the two species by holding gravid females in captivity …

Characterization Of Unusual Gymnamoebae Isolated From The Marine Environment, Margaret Wacera Mbugua

Theses, Dissertations and Capstones

Amoebae are important heterotrophic protists (protozoa) and this study focuses on three unusual forms from the marine environment. Amoebae are ubiquitous and play an important role as consumers in microbial communities. A common coastal ctenophore (Mnemiopsis sp.) is known to harbor an undescribed naked amoeba on the comb plate surface. The nature of the symbiotic association is unknown although electron microscopy suggests these micrograzers are degrading comb plates (Moss et al., 2001). A second amoeba isolate from mangrove waters is new to science by virtue of its distinctive trophic form that does not conform to any described species. …

Effects Of Aging And Gender On Regulators Of Muscle Adaptation In F344/Bn Rat Model, Satyanarayana Paturi

Theses, Dissertations and Capstones

Sarcopenia is the loss of muscle mass and strength that occurs with aging. Here we examine the effects of aging and gender on the regulation of molecules believed to regulate muscle growth and adaptation in the F344/BN rat. In male animals, soleus and EDL muscle/body weight ratio declined continuously with aging while muscle atrophy in female animals plateaued at 26-months and remained constant thereafter. Aging increased the phosphorylation of protein kinase-B (Akt) and the mammalian target of rapamycin (mTOR) in the female but not male soleus muscle. This finding was associated with the attenuation of muscle atrophy observed in female …

West Virginia Streamside Salamander Guilds And Environmental Variables With An Emphasis On Pseudotriton Ruber Ruber, Kathryn Rebecca Pawlik

Theses, Dissertations and Capstones

Amphibian distributions are greatly influenced by environmental variables, due in part to semi-permeable skin which makes amphibians susceptible to both desiccation and toxin absorption. This study was conducted to determine which streamside salamander species were sympatric and how environmental variables may have influenced habitat choices. One hundred sixty streams were surveyed throughout 55 counties in West Virginia during the summer of 2007. At each site, a 10 m ² quadrat was established around a central aquatic habitat. While surveying, I looked under natural cover objects, in vegetation, and through leaf litter. I recorded species, cover object type, and location on …

Terrestrial Habitat Use Of Marbled Salamanders Ambystoma Opacum: A Site Specific Approach, Frank Piccininni

Theses, Dissertations and Capstones

Habitat destruction, fragmentation and degradation have contributed heavily to the decline of amphibian populations such as Marbled Salamanders (Ambystoma opacum). Often, ambystomatid water resources are conserved without consideration for the equally important terrestrial habitat. This is partly due to a lack of information regarding the relationship between ecological succession, plant community composition, microhabitat and salamander abundance. Three sampling transects consisting of drift fence arrays, vegetation assessments and microhabitat surveys were extended 100 m into the terrestrial habitat surrounding a seasonal wetland at Beech Fork State Park in Wayne County, West Virginia. Principal components analysis was used to identify …

A Continued Study Of The Use Of Created Ponds For Amphibian Breeding In Fragmented Forested Areas, Amy Elizabeth Schneider

Theses, Dissertations and Capstones

Amphibian populations are declining worldwide due to factors such as habitat degradation, fragmentation and destruction. I conducted a study to explore the use of created ponds in a forested habitat by breeding amphibians, specifically Rana sylvatica and Ambystoma maculatum. The objectives were to examine the movement of these animals after leaving the ponds, the survival and movement of juveniles, how both respond to fragmentation, and how similar the created ponds were to natural ones. Nine ponds were constructed in December 2003 in the MeadWestvaco Wildlife Ecosystem Research Forest (MWERF) in Randolph County, West Virginia. All trapped amphibians were measured and …

The Natural History And Distribution Of The Mountain Earthsnake (Virginia Valeriae Pulchra) In West Virginia, Daniel Ware

Theses, Dissertations and Capstones

The Mountain Earthsnake, Virginia valeriae pulchra, has received little attention in the literature to date. It is imperiled in West Virginia with only 6 to 20 populations known throughout the Allegheny Mountain and Ridge and Valley Physiographic provinces. Eighty snakes were collected during the 2006 and 2007 summers. Typical habitat is open fields with short grass, flat to moderate slopes that have scattered fine sandstone rocks near a source of water and forest edge. Fine sandstone rocks were the primary cover objects used. Snakes were sexually dimorphic with males having longer tails expressed as a percent of total body lengths …

Comparing Digit Morphology Of An Arboreal Salamander With Potential Competitors, Eric Hugh Diefenbacher

Theses, Dissertations and Capstones

Green Salamanders (Aneides aeneus) are the only salamanders in West Virginia to exhibit an arboreal lifestyle. The focus of this study was to determine the cellular anatomy of the distal digit structures and how these structures may influence climbing ability. Comparative histology, comparative morphometrics, and comparative osteology of Green Salamanders, Cumberland Plateau Salamanders (Plethodon kentucki), and Slimy Salamanders (Plethodon glutinosus), were also used to determine if Cumberland Plateau Salamanders had the potential to compete with Green Salamanders for arboreal habitats. Histologically, Cumberland Plateau Salamanders had cell layer thickening similar to that of Green Salamanders. Morphometrically, Green Salamanders and Cumberland Plateau …

Habitat Comparison Of Pseudacris F. Feriarum And Pseudacris C. Crucifer With Emphasis On Associated Plant Communities And Distribution Of Clemmys Guttata And Pseudacris F. Feriarum In West Virginia, Scott Joseph Albaugh

Theses, Dissertations and Capstones

Upland Chorus Frogs (Pseudacris f. feriarum) are a rare species in West Virginia. They occur in aquatic habitats across parts of the eastern and southern U.S. Pseudacris f. feriarum collection records in West Virginia suggest that their distribution is limited to the eastern panhandle and Ridge and Valley region where they are very rare and imperiled. Distribution information is needed for future management strategies. To help conserve this rare species, key life history habits were identified through the investigation of plant communities that are associated with them. Data on Pseudacris f. feriarum distribution in West Virginia was determined by conducting …

Factors That Influence The Distribution Of Antibiotic Resistant Bacteria In The Mud River, Wv, Timothy Shaun Dotson

Theses, Dissertations and Capstones

Background: Prior studies of the Ohio and Mud Rivers have shown that fecal contamination alone does not explain the distribution of antibiotic resistant bacteria (ARB) in surface waters. The objectives of this study were to determine effects on the distribution of ARB in the Mud.

Methods: Water samples and physical parameters were collected twice per season in spring through fall at 12 sites on the Mud River. Aliquots were plated on R2A agar and R2A agar plus individual antibiotics for the enumeration of total cultivable bacteria, and cultivable bacteria resistant to ciprofloxacin, virginiamycin, or tetracycline respectively. The IDEXX Quanti-Tray/2000™ method …

A Taxonomic Study Of The Genus Acris And The Status Of Acris Crepitans Blanchardi (Harper), Blanchard's Cricket Frog, In Southern Ohio And Western West Virginia, Amy M. Hamilton

Theses, Dissertations and Capstones

Distributions of Acris crepitans and Acris gryllus cover most of the eastern United States with some overlap, and the subspecies Acris crepitans blanchardi and Acris crepitans crepitans also occur sympathric in parts of their ranges. My study analyzed 15 morphological characteristics of A. c. blanchardi, A. c. crepitans, and A. g. gryllus using Canonical Discriminate Analysis. Results support current taxonomy, showing the most separation between A. crepitans and A. gryllus and some overlap between A. c. blanchardi and A. c. crepitans. Sexual dimorphism was seen only in A. c. blanchardi and A. c. crepitans with females being larger …

A Study On The Population Size And Natural History Of The Eastern Six‐Lined Racerunner, Aspidoscelis Sexlineata, In West Virginia, With Notes On Other Lizard Species, Emmy Johnson

Theses, Dissertations and Capstones

Species will expand ranges through natural or human‐alterations. Such an occurrence has happened in West Virginia with Eastern Six‐lined Racerunner, Aspidsocelis sexlineata, a species that has been found to thrive in areas of high disturbance from humans. While found abundantly in other states, they are thought to occur only in the eastern panhandle of West Virginia (Morgan County) where they are believed to have come from Maryland via a railroad bridge. Without the bridge, the Potomac River forms a natural barrier between these two states. The objectives of my study were to determine the population size and natural history …