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Articles 1 - 7 of 7
Full-Text Articles in Life Sciences
Performance Of A Dna And Protein Co-Extraction With Trypsin In Comparison To Two Proteinase K Based Dna Extraction Methods, Dinura A. Gunatilake
Performance Of A Dna And Protein Co-Extraction With Trypsin In Comparison To Two Proteinase K Based Dna Extraction Methods, Dinura A. Gunatilake
Student Theses
Effective DNA extraction methods are important for forensic applications. The main goal of this experiment was to determine if a newly developed trypsin based protein/DNA co-extraction method applied to contact traces would yield comparable results to a commercial Proteinase K method (QIAamp DNA Investigator Kit) and a Chelex extraction method with Tween 20 used in forensic laboratories. This was tested on 20 sets of sebaceous fingerprints on glass slides.
The results of this study demonstrate the trypsin co-extraction method yielded the highest amount of DNA. In the first comparison, the mean total DNA yields for the trypsin-co extraction method and …
Further Evaluation Of A Dry Vacuuming Technique For Recovery Of Dna From Handwritten Documents, Christian Hopkins
Further Evaluation Of A Dry Vacuuming Technique For Recovery Of Dna From Handwritten Documents, Christian Hopkins
Student Theses
A previously developed, non-destructive, homemade vacuum method for collecting biological material from handwritten documents had promising DNA results for white copy paper, preserved indented writing, and latent prints. Prior to casework implementation, additional validation experiments are warranted and here the method was tested for different paper substrates. This work describes testing of notebook paper, bank deposit slips, magazine pages, and manila envelopes. The quantity of recovered DNA varied from donor to donor, but the mean quantities showed a trend that can be explained by the different sizes and surface properties of the tested paper types. The rougher paper type, like …
Method Development For Identifying Counterfeit Cigarettes Using Environmental Pollen Analysis, Tiffany Chan
Method Development For Identifying Counterfeit Cigarettes Using Environmental Pollen Analysis, Tiffany Chan
Student Theses
Cigarette counterfeiting negatively impacts government revenue and undermines tobacco control policies. Tracking geographical origins and potential trade routes of counterfeit cigarettes is important for investigation illegal trade in tobacco products. Traditional pollen extraction techniques provide clear residues of pollen trapped on tobacco leaves and used for geographical identification; however, such methods are time-consuming and utilize concentrated chemicals. This study developed a simple, fast and environmentally friendly method for extraction of pollen grains used in differentiating genuine and counterfeit cigarettes. Experimental factors that may affect pollen recovery including number of cigarettes, extraction solution, and extraction temperature, were optimized. The optimum parameters …
Discrimination Of Monozygotic Twins Using Dna Methylation Levels Of One Cpg Site At Chromosome 3, Dino O. Robinson
Discrimination Of Monozygotic Twins Using Dna Methylation Levels Of One Cpg Site At Chromosome 3, Dino O. Robinson
Student Theses
Conventional STR typing, commonly used in forensics for human identification, poses a problem in criminal cases and paternity disputes involving monozygotic (MZ) twins because they share identical DNA sequences. To date, no routine method is available in forensics to differentiate between individuals of MZ pairs. Recently, epigenetic methods measuring differential DNA methylation patterns have been applied to MZ twin differentiation. In this study, we investigated the potential to identify MZ twins using a previously identified DNA methylation site in chromosome 3, cg18562578, in a sample of 129 MZ and 37 dizygotic (DZ) twin pairs. We used bisulfite converted saliva DNA …
Identification And Quantification Of Classic, Prescription, And Synthetic Opioids In Hair By Liquid Chromatography Tandem Mass Spectrometry (Lc/Ms/Ms), Natalia A. Platosz
Identification And Quantification Of Classic, Prescription, And Synthetic Opioids In Hair By Liquid Chromatography Tandem Mass Spectrometry (Lc/Ms/Ms), Natalia A. Platosz
Student Theses
The current use and misuse of synthetic and prescription opioids in the US has reached epidemic status. According to the US Department of Health and Human Services, every day more than 130 people in the US die after overdosing on opioids, and 2.1 million had an opioid use disorder in 2016. Hair is becoming an alternative matrix of increasing interest in forensic toxicology to investigate drug use and abuse patterns due to its long window of detection. The focus of this project was to develop and validate a method that simultaneously detects and quantifies 27 classic, prescription and synthetic opioids …
Analysis Of Cannabinoids In Serum By Gc-Ms/Ms, Christie Cannarozzi
Analysis Of Cannabinoids In Serum By Gc-Ms/Ms, Christie Cannarozzi
Student Theses
Due to recent changes in federal and state legislations, the availability and consumption of cannabis products have increased in the United States. The expanded use of recreational and medicinal cannabis products increases the importance of implementing sensitive and selective instrumental methods in toxicological laboratories, as legal implications may arise in forensic cases, such as driving under the influence of drugs (DUID). The purpose of this study was to perform a cross-validation for the quantitative analysis of cannabinoids (Δ9-tetrahydrocannabinol, cannabidiol, cannabinol, 11-hydroxy-Δ9-tetrahydrocannabinol, and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol) in serum by gas chromatography coupled to triple quadrupole tandem mass spectrometry (GCTQ). This method was fully …
Seeing Health: Examining The Association Between Hispanic Caribbean Restaurant Characteristics And Healthy Menu Images In New York City, Eddie N. Sakowitz
Seeing Health: Examining The Association Between Hispanic Caribbean Restaurant Characteristics And Healthy Menu Images In New York City, Eddie N. Sakowitz
Student Theses
Hispanics experience more diet-related health disparities in comparison to non-Hispanic whites. Community nutrition environments can influence health outcomes, but restaurants are a largely untapped research area. This study examined how Hispanic Caribbean restaurants promote healthy eating through menu design, and which Hispanic Caribbean restaurants characteristics are associated with healthy menu images. We hypothesize that healthy menu images will be associated with more affluent neighborhoods. We examined the nutrition environment in a random sample of 89 Hispanic Caribbean restaurants in New York City. This analysis included a subsample of Hispanic Caribbean restaurants that had menus with images (n=51). Hispanic Caribbean restaurants …