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Full-Text Articles in Life Sciences
Structural And Calcification Patterns Of The Neonatal Line In The Enamel Of Human Deciduous Teeth, Tetsuo Kodaka, Tsuneyoshi Sano, Shohei Higashi
Structural And Calcification Patterns Of The Neonatal Line In The Enamel Of Human Deciduous Teeth, Tetsuo Kodaka, Tsuneyoshi Sano, Shohei Higashi
Scanning Microscopy
The neonatal line of the enamel in human deciduous teeth was observed by scanning electron microscopy using secondary and backscattered electron signals. The neonatal line containing irregular structures of enamel prisms with disordered crystal arrangements was basically formed by the abrupt bending of the prisms towards the root. Usually, the prisms gradually bent back again to regain their previous orientation, but the prisms in the inner and the surface layer sometimes ran straight ahead after bending. The prism sheath regions showing hypocalcification contained a relatively large amount of organic material as shown by treatment with chromium sulfate. When etched with …
A Novel Method For Viewing Heavy Metal Stained And Embedded Biological Tissue By Field Emission Scanning Electron Microscopy, R. Geoffrey Richards, Iolo Ap Gwynn
A Novel Method For Viewing Heavy Metal Stained And Embedded Biological Tissue By Field Emission Scanning Electron Microscopy, R. Geoffrey Richards, Iolo Ap Gwynn
Scanning Microscopy
Backscattered electron (BSE) imaging was used to display heavy metal stained biological structures of various embedded specimens. Samples were fixed, stained and embedded in resin blocks as with preparation for the transmission electron microscope (TEM). Blocks were trimmed to center the specimens in a trapezoidal face of up to 5 mm2 and their sides painted with conductive silver paint leaving the face uncovered. Blocks were sputter coated with 6-8 nm of silver, chromium or aluminum, with aluminum providing the best specimen contrast in BSE mode. Samples were examined in a field emission scanning electron microscope operated at a high …