Life Sciences Commons^™

Β-Cyclodextrin-Enzalutamide Self-Assembly Complexes For Prostate Cancer Therapy, Meghana Kolli, Rahul Tiwari, Subhash C. Chauhan, Yonghong Zhang, Neeraj Chauhan, Alba C. M, Murali Yallapu

Research Symposium

Background:Castrate circumstances brought on by therapy give rise to castrationresistant prostate cancer (CRPC), which thereafter develops androgen receptor (AR) resistance. AR inhibitors are frequently used to obstruct AR translocation in cells that overexpress AR and slow the progression of cancer by reducing the number of AR receptors that are accessible. In comparison to Bicalutamide, a first-generation AR inhibitor, the hydrophobic drug Enzalutamide has demonstrated higher effectiveness. We investigate Enzalutamide as a viable option for the formation of self-assembly complexes because AR downregulation is still the major treatment for CRPC.

Methods:Enz-loaded self-assembly complexes (enz-β-CD) formulation was developed using solvent …

Update On The Role Of Muc13 In Pancreatic Cancer: A Promising Early Detection Biomarker, Anupam Dhasmana, Swati Dhasmana, Sheema Khan, Meena Jaggi, Murali M. Yallapu, Subhash C. Chauhan

Research Symposium

Background: With the rise in pancreatic cancer (PanCa) prevalence and mortality rate, by 2030 it will secure second position among leading causes of cancer-related deaths. Due to poor prognosis of PanCa only 11% of PanCa patients have a 5-year survival rate, resulting in an equal mortality rate and incidence rate. 85% of PanCa are Pancreatic ductal adenocarcinoma (PDAC). The main clinical challenge with PanCa is poor treatment outcomes due the late diagnosis. Currently, there are traditional biomarkers panels available for diagnosis, however, these biomarkers do not have optimal sensitivity and specificity for PanCa. Considering this alarming unmet clinic need, our …

Ultrabithorax, Abdominal-A And Abdominal-B Form Heterodimers And Homodimers In Living Cells By Bifc, Rubén Montalvo Méndez, Gustavo Jiménez-Mejía, Diana Reséndez-Pérez

Research Symposium

Background: Hox genes encode transcriptional factors that regulate the expression of specific target genes along the anterior-posterior axis determining the segment identity during embryonic development. These master genes are expressed in the same order in which are located in the third chromosome of Drosophila melanogaster. As it was previously reported the interaction of neighboring Hox proteins as Scr-Antp and Antp-Ubx and one of the mechanisms for Hox protein regulation is protein-protein interaction, we determined the heterodimer formation of Ubx with AbdA and AbdB as well as the homodimer formation of these proteins in living cells which in turn could …

Trip13’S Crucial Role In Pancreatic Cancer Progression, Swati Dhasmana, Anupam Dhasmana, Stella Rios, Iris A. Enriquez-Perez, Sheema Khan, Farrukh Afaq, Upender Manne, Murali M. Yallapu, Subhash Chauhan

Research Symposium

Background: Pancreatic cancer, characterized by its high mortality rate, stands as one of the most aggressive cancer forms. The projected surge in pancreatic cancer-related deaths, making it the second leading cause in the United States by 2030, underscores the urgency for effective early screening tools. This study employs data mining methods to scrutinize bioinformatic data surrounding TRIP13. Examining differential expression across various cancers, correlating TRIP13 expression with pancreatic cancer stages, exploring associations with common cancer genes, and analyzing overall survival rates constitute the core investigations. Integrated with molecular biology techniques, the study further quantifies TRIP13 expression in progressive pancreatic cancer …

Targeting Mycobacterial Efflux System For Combating Anti-Microbial Resistance, Arathi Radhakrishnan, Raj Kishor Kapardar, Rajpal Srivastav

Research Symposium

Background: The drug resistance in the microbes is a serious concern in medicine. Along with intrinsic factors, extrinsic factors like unprescribed usage of drugs are the contributing factors. The drug tolerance has led to the emergence of superbugs. Mycobacterial species utilize an array of multidrug efflux mechanisms linked to intrinsic and acquired antibiotic resistance. Understanding molecular mechanisms regulating efflux could reveal new therapeutic targets and strategies. Our study is aimed to target regulators of efflux Mycobacterial transporter.

Methods: Using the reference mycobacterial strain, antibiotic sensitivity was first profiled by minimal inhibitory concentration assays across a panel of antimicrobials, followed by …

Stress Hormone And Heart Rate Responses To Various Exercise Training Methods, Tomas Gomez Jr., Rebekah Schlatter, Megan Zamora, Marco Arriago, Sue Anne Chew, Murat Karabulut

Research Symposium

Background: Blood Flow Resistance (BFR) training has garnered attention for its ability to induce positive physiological adaptations with low-load resistance exercise. The present study aimed to examine the responses of catabolic hormones and heart rates (HR) to various BFR training protocols. This investigation seeks to provide insights into the stress levels induced by different protocols and identification behind the most effective protocol for optimal positive exercise-related adaptations.

Methods: Study population involved 10 healthy adult males (height: 175.0±5.0 cm, weight: 96.67±26.6 kg, age: 21.3±2.67 yr.) in a five-session investigation. Informed consent paperwork, pre-testing and anthropometric measurements served as session 1; sessions …

Proteomic Analysis Of Stress Associated Factor Overexpression In Hepatocellular Carcinoma, Mohammad Shabir Hussain, Sophia Leslie, Amayrani Sanchez, Samantha Lopez, Kyle Doxtater, Manish Kumar Tripathi

Research Symposium

Background: Hepatocellular carcinoma (HCC) constitutes a substantial portion, accounting for 85% to 90% of liver cancers worldwide. Notably, within the Hispanic population, liver cancer mortality rates are notably higher, particularly evident in regions like the South Texas Rio Grande Valley (RGV), where nearly 90% of the populace is Latino/Hispanic. This region grapples with poverty affecting nearly 30% of its residents, coupled with elevated rates of obesity, diabetes, and low-income households, thereby fostering a prevalent environment of stress. Stress can profoundly impact cancer outcomes by compromising immune functionality and triggering inflammatory responses, potentially impairing surveillance against oncogenic triggers. The activation of …

Piperlongumine Nanoformulation Attenuates Pancreatic Tumor Desmoplasia And Alter Tumor Immune Responses, Vivek Kumar Kashyap, Neeraj Chauhan, Mohammed Sikander, Eswara N. H. K. Ghali, Bilal Hafeez, Murali M. Yallapu, Meena Jaggi, Subhash C. Chauhan

Research Symposium

Pancreatic cancer (PanCa) is characterized by lack of early diagnosis, poor response to available therapeutic modalities and chemoresistance. Gemcitabine (GEM) is currently considered the most effective therapy for PanCa; however, it shows only a marginal survival benefit of 6 months. This poor drug response has been attributed to desmoplasia, causes suboptimal drug delivery, alters tumor microenvironment (TME), which includes tumor surrounding blood vessels, fibroblasts, immune cells, extracellular matrix, and other signaling molecules and induces chemo-resistance in tumors. To overcome these existing issues associated with chemotherapy, identification and development of novel therapeutic modalities are a pressing need. Piperlongumine (PL) is a …

Ongoing Study Of The Association Of Apoe Gene Polymorphisms With Cognitive Impairment In The Rio Grande Valley Hispanic Population, Jaime Miguel L. Saveron, Daniela Gamez, Chun Xu

Research Symposium

Background: Alzheimer's disease (AD), a prevalent neurodegenerative disorder, exhibits a significant genetic component. In addition, mild cognitive impairment (MCI) is a key risk factor for AD. While certain demographic factors and genetic variants associated with AD and cognitive impairment have been identified in non-Hispanic populations, limited research exists on this subject within the Hispanic population, particularly in the Rio Grande Valley. This study aims to investigate the relationship between the APOE gene, specifically its ε4 allele, and cognitive impairment in the Hispanic population of the Rio Grande Valley.

Methods: A total of 269 Hispanic subjects, aged 60 and above, with …

La1-Xsrxcoo3 Perovskite Nanomaterial: Synthesis, Characterization, And Its Biomedical Application, Adhira Tippur, Anyet Shohag, Luke Franco, Ahmed Touhami, Swati Mohan, Mohammed Uddin

Research Symposium

Early cancer detection is paramount for effective treatment and potential cures. This research explores the application of perovskite materials, specifically Sr2+-doped Lanthanum Cobaltite (La1-xSrxCoO3) nanomaterials, in cancer detection, with a focus on rats as an experimental model. The ferroelectric nature of these materials, synthesized through a combination of sol-gel and molten-salt processes, was examined at varying Sr2+ doping levels (1-20 wt%). Rigorous characterization, employing X-ray diffraction and scanning electron microscopy, confirmed the uniform morphology of nano cubes, laying the foundation for subsequent investigations. The magnetic properties of the perovskite nanoparticles were probed, suggesting their potential as a diagnostic tool for …

Investigating Interrater-Reliability In Assessing Social Behavior Of Monodelphis Domestica, Bianca A. Camacho, Johanna Perez, Jatziry L. Escobedo, John L. Vandeberg, Mario Gil

Research Symposium

Background: Reliable, consistent, and objective data is a goal all studies aim to achieve, but many struggle to obtain when subjective biases between researchers can occur. Inter-rater reliability (IRR) is a statistical measure used to quantify the degree of agreement between researchers qualitatively scoring the same phenomenon. The primary goal of this study is to enhance the methodology used to achieve optimal IRR. Using an established ethogram, our team of researchers scored the social behavior of the adult gray short-tailed opossum (Monodelphis domestica) to propose an effective method for achieving high IRR that can contribute to future …

Honey Targets Ribosome Biogenesis Process In Human Pancreatic Cancer Cells To Inhibit Their Growth And Metastatic Phenotypes, Aun A. Bangash, Muhammad Bangash, Haider Ahsan, Shiza Khan, Mudassier Ahmad, Dae Joon Kim, Sahir Alvi, Bilal B. B. Hafeez

Research Symposium

Background: Pancreatic cancer (PanCa) is the fourth deadliest cancer worldwide and is expected to become the second deadliest cancer by 2030. In the USA, the National Cancer Institute put forth a grim prediction stating that there will be 64,050 new cases in 2023 alone and about 50,000 of these patients will die. Existing therapeutic regimens against PanCa are not that effective and show unacceptable toxicities. Therefore, developing highly effective new agents with less toxicity is urgently required, which could be used as a monotherapy or as an adjuvant to treat PanCa patients. Honey is known for its tremendous health benefits …

Exploring Neuroplasticity Changes In Neurotoxin-Induced Parkinson’S Disease: A Preliminary Analysis Using Transcranial Magnetic Stimulation, Tomas Gomez Jr., Daniel Salinas, Kelsey Potter-Baker, Nawaz Hack, Ramu Vadukapuram

Research Symposium

Background: Parkinson’s disease (PD) is a neurodegenerative condition that affects movement, cognition, gait, and significantly impacts one's quality of life. Studies have suggested that neurotoxin pre-exposure is related to PD pathology and progressive motor/non-motor deficits, though it remains unclear how neurotoxin exposure affects neuroplasticity. The present study aimed to examine neurotoxin–induced PD-associated neuroplasticity changes in relationship to mental acuity and PD motor functionalities.

Methods: 7 voluntary participants experiencing early-stage PD symptoms with self-reported neurotoxin pre-exposure were enrolled in the longitudinal, repeated-measures clinical study; 2 sex-matched, age-matched, and occupation-matched healthy subjects were recruited for controlled comparative analysis (n=9). UTRGV’s Institute …

Examining Cetp Gene Associated With Ad-Related Diseases Of The Hispanic Population In The Rio Grande Valley., Erika Guajardo, Luis Aguillon, Daniela Ollervides-Charles, Kesheng Wang, Gladys E. Maestre, J. Garza, Chun Xu

Research Symposium

Background: There are currently about 6 million people in the United States that suffer from Alzheimer’s Disease (AD) and Alzheimer’s Disease related dementia (ADRD). It is a progressive disease beginning with mild memory loss and possibly leading to loss of the ability to carry on a conversation and respond to the environment. Over time, these conditions can cause many different health issues that decrease the quality of life. In addition, Hispanic people are twice as likely to develop AD or AD related dementia than non-Hispanic White people. In our study, we are investigating a known gene, CETP, that directly corresponds …

Diosgenin Prevents Breast Cancer Metastasis Via The Inhibition Of Epithelial-Mesenchymal Transition., Santosh K. Singh, Rajesh Singh

Research Symposium

Background: Globally, breast cancer (BrCa) is the primary cause of cancer-related morbidity and death in women. Despite significant changes in healthcare activities like screening and early detection over the past few decades, African Americans (AA) continue to experience cancer health disparities. Many studies have been done on BrCa treatments, but AA patients have had less success than other racial or ethnic groups. Therefore, novel strategies are required to improve survival rates, lower BrCa mortality, and ultimately enhance the health of racial/ethnic minorities. Current treatment regimens, such as chemotherapeutic agents, are showing less effectiveness since they are linked to drug resistance, …

Evaluation Of Ergosterol And Its Metabolites As Lxr Agonists And Their Anticancer Potential In Colon Cancer, Yogain Taank, Navneet Agnihotri

Research Symposium

Purpose: Aberrant cholesterol homeostasis is a well-recognized hallmark of cancer and implicated in metastasis and chemotherapeutic resistance, the two major causes of cancer associated mortality. Liver X receptors (LXRs) are the key transcription factors that induce cholesterol efflux via enhancing the expression of ABCA1 and ABCG1.

Methods: Molecular docking and dynamic simulation studies were done to assess the binding affinity and stability of the receptor ligand complexes. Activation of LXRs was evaluated using the luciferase reporter assay. qRT-PCR and western blotting was done to analyse the mRNA and protein expression of cholesterol homeostasis genes. Flow cytometric analysis was carried out …

Alteration Of The Interferon Alpha Signaling Pathway Significantly Affects Alzheimer's Disease Pathology In App/Ps1 Mouse Model., Ranjit Kumar Das, Nirakar Sahoo, Chun Xu, Hansapani Rodrigo, Michael Paul Murphy, Upal Roy, Deepa Roy

Research Symposium

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder characterized by cognitive impairment, memory loss, and disturbances in behavior. The exact cause of the disease is unknown but there is evidence of molecular pathways alteration within the brain. Many molecular mechanisms have been studied for the progression of AD. Nonetheless, the contribution of the signal transducer and activator of transcription 1 (STAT1) and interferon-induced protein with tetratricopeptide repeats 3 (IFIT3) genes have not been well established in AD pathology. This study aimed to investigate the contribution of STAT1 and IFIT3 in the BALB/C and APP/PS1 mouse models. We analyzed the STAT1 …

A Potential Role Of Urinary P75ecd As A Biomarker For Amyotrophic Lateral Sclerosis In An American Cohort, Swati Dhasmana, Anupam Dhasmana, Sheema Khan, Acharan S. Narula, Murali Yallapu, Subhash Chauhan

Research Symposium

Background: Neurological disorders present a unique complexity compared to other diseases, involving multiple risk factors, causes, treatments, and outcomes. These disorders often exhibit various molecular and morphological changes indicative of disruptions in cellular plasticity and resilience. The pathogenesis of many neurological disorders remains unclear, necessitating ongoing investigations. Amyotrophic lateral sclerosis (ALS) exemplifies an idiopathic and fatal neurodegenerative disease marked by the degeneration of upper and lower motor neurons. The average life expectancy post-diagnosis is a mere 36 months, primarily attributed to respiratory muscle denervation.The persistent challenges in ALS clinical trials and the absence of effective therapeutic options have intensified interest …

Healthy Media - Enhancing Healthy Living Education In Prek Students With Edtech Media, Emilio Puente Barrios

Research Symposium

Background: Young children today learn in diverse environments, including home, school, and through digital media platforms such as apps, videos, games, and songs. With the increase in screen time due to Covid-19 restrictions, digital media has become a valuable resource for remote learning, offering fun and educational interactions. However, the impact of incorporating educational technology or EdTech digital media applications into health education curricula on the performance of PK 4 students in understanding and adopting healthy living concepts remains understudied. By exploring the effectiveness of design elements and strategic approaches this research aims to evaluate newer technology-driven media in enhancing …

Effective Management And Implementation Of A Coordinated Health Program - The Steps Snap-Ed Experience, Moises Cisneros, Brenda Rodriguez-Guerra, Joseph Reyes, Emilio Puente Barrios

Research Symposium

Purpose: Coordinated health programs have been proposed for more than four decades to improve the health of children in school settings. Although a challenging task, it has the potential to touch many lives, especially those from impoverished communities (Darlington, Violon, & Jourdan, 2018). The South Texas Early Prevention Studies (STEPS) aim to positively impact the community of the Rio Grande Valley, a population known to have a high rate of obesity, by implementing a comprehensive and proactive approach with various components within a preschool students’ life. The project's goal has been to plant a seed at an early age for …

Tox3 Rs3803662 Polymorphism Is Associated With Breast Cancer Protection In Northeastern Mexican Woman, Orlando D. Solis-Coronado, Hazyadee F. Rodríguez-Gutiérrez, Monica P. Villarreal-Vela, Ricardo M. Cerda-Flores, Juan F. González-Guerrero, Oscar Vidal-Gutiérrez, Diana C. Pérez-Ibave, Maria Lourdes Garza-Rodríguez

Research Symposium

Introduction: Low penetrance genes are involved in breast cancer (BC) and confer risk for the development of this neoplasia. Different single nucleotide polymorphisms (SNPs) associated with BC have been identified, such as rs3803662 (TOX3), which is related to estrogen receptors in European and African-American women. The contribution of this variant in the Mexican population is unknown. The objective of this study was to evaluate, through a case-control design, the association of the SNP rs3803662 (TOX3), with the risk of BC in women from northeastern Mexico.

Methods: We included 434 cases and 228 controls. Genotyping was carried out using RFLPs. The …

Understanding An Inflammatory Pathway In Diabetic Retinopathy, Reanna R. Rodriguez

Research Symposium

Diabetic Retinopathy (DR) is the leading cause of blindness in the U.S. However, not much is known of its molecular pathway and how it attributes to increases in inflammatory response in the eye. One avenue we will investigate is the transforming growth factor beta (TGFB) signaling pathway and its effect of vascular endothelial growth factor (VEGF) secretion and cell viability. VEGF is the hallmark that exacerbates DR progression in prolonged diabetes. Some major concern that have arisen are the underlying effects of oxidants and antioxidants in elevating VEGF secretion in diabetes. In attempt to learn more, we evaluated how an …

Trimeric Complex Interactions Of Antp-Tbp With Tfiieb And Exd Are Involved In The Genetic Control Of Drosophila Melanogaster, Gustavo Jiménez Mejía, Ruben De Jesus Montalvo Mendez, Claudia Dalila Altamirano Torres, Diana Reséndez Pérez

Research Symposium

Background: Homeoproteins are transcriptional factors (TFs) that shape animal body axes during development. These TFs are highly conserved and represent one of the most fascinating groups of regulatory molecules. Reports shown the multiplicity of interactions in hox proteins, as complexes trimeric involved to transcriptional activity. The study of trimeric complexes in Hox interactome will allow the better understanding of Hox genetic regulation during embryonic development.

Methodology: Using a new combination BiFCFRET approach performed in HEK293, the quantification was performed by FRETTY of ImageJ. Fly crosses were incubated at 25ºC on standard yeast-agar-cornmeal medium. Embryo cuticle preparations were carried out according …

Therapeutic Efficacy Of Ormeloxifene Against Hepatocellular Carcinoma, Mohammed Sikander, M. Vela, Shabnam Malik, A. Rodriguez, A. Lopez, Daniel Zubieta, Fathi T. Halaweish, Subhash C. Chauhan

Research Symposium

Background: Hepatocellular carcinoma (HCC) is the most common primary liver cancer and leading cause of cancer related deaths worldwide. Severe toxicity and drug resistance to available chemotherapeutic agents display ineffective clinical response. Therefore, drug repurposing is gaining attention owing to their known biological activities and excellent safety profiles. Ormeloxifene (ORM), non-steroidal, selective estrogen receptor modulator (SERM), and exhibit diverse pharmacological activities. The aim of this study is to assess the therapeutic activity of ORM and to investigate the underlying molecular mechanism against hepatocellular carcinoma.

Objective: To investigate the therapeutic activity of ormeloxifene in human hepatocellular carcinoma cells. Methodology: MTT and …

The Intensity Of Physical Activity Improves Cognitive Performance Among Aging Americans, Imtiaz Masfique Dowllah, Juan Carlos Lopez-Alvarenga, Gladys E. Maestre, Ulku S. Karabulut, Murat Karabulut

Research Symposium

Background: Currently there is no pharmacological cure for Alzheimer’s disease and related dementias, physical activity (PA) has emerged as a promising approach. The optimal intensity of PA to improve cognitive health remains unknown. Therefore, this study aimed to evaluate associations between different durations and intensities of PA on performance across cognitive domains (executive function, processing speed, and memory) among aging Americans.

Methods: 2377 adults aged ≥ 60 years from the cross-sectional National Health and Nutrition Examination Survey 2011-2014, were included. Linear regression in hierarchical blocks and the size of effect (η2) were analyzed with R software.

Results: The mean age …

The Impact Of Biological Sex On Motor Function And Responses To Novel Environments In The Gray Short-Tailed Opossum (Monodelphis Domestica), Esperanza I. Alaniz, Ismael Perez, Sasawan Heingraj, Katelynn Renteria, Cristian M. Botello, Joseph C. Cantu, John L. Vandeberg, Mario Gil

Research Symposium

Background: Understanding the different stress reactions in different environments can help us understand stress factors. Studying animal behavior is important for translational research for mental health improvement.

Previous literature has shown that stress is a risk factor for higher cancer incidence and poorer cancer survival. (Klejbor & Turlejski., 2012), as well as mental health outcomes. Understanding of how stress is related to cancer can help improve therapeutic outcomes as preventive measures (Glaser et al., 1987).

Methods: Using the Rota Rod apparatus, 12 animals (3 males, 9 females) were tested at 36 rpm for a maximum of 400 seconds (Madroñal et …

Tc-Ptp Overexpression Attenuates Skin Cancer Formation During Environmental Skin Carcinogenesis, Zahidur Rahmann, Carson Bogatto, Serena Olivarez, Dae Joon Kim

Research Symposium

Background: T-cell protein tyrosine phosphatase (TC-PTP), encoded by Ptpn2, has been shown to function as a tumor suppressor during skin carcinogenesis.

Methods: we generated a novel epidermal specific TC-PTP-overexpressing (K5HA.Ptpn2) mouse model to show that TC-PTP contributes to the attenuation of chemically induced skin carcinogenesis through the synergistic regulation of STAT1, STAT3, STAT5, and PI3K/AKT signaling.

Results: We found overexpression of TC-PTP increased epidermal sensitivity to DMBA-induced apoptosis and it decreased TPA-mediated hyperproliferation, coinciding with reduced epidermal thickness. Inhibition of STAT1, STAT3, STAT5 or AKT reversed the effects of TC-PTP overexpression on epidermal survival and proliferation. Mice overexpressing TC-PTP in …

Targeting Cellular Signaling Pathways In Cancer By Lactobacilli, Varish Ahmad, Aftab Ahmad, Quazi Muhammad Sajid Jamal

Research Symposium

Purpose: Presenting lactobacilli therapy for cancer treatment targeting cancer signaling Lactobacilli as Probiotic lactic acid bacteria (LAB) are a group of fermentative gram-positive and gram-negative bacteria that produces a large number of intracellular and extracellular metabolites used in the food manufacture industry as well as complementary and alternative medicines against many diseases including cancer.

Description: Some LAB has been found to have inhibitory activity against colon liver cancer, cancer, colorectal cancer, breast cancer, and lung cancer in vivo or in vitro. These fermentative bacteria induced the autophagy cell death either by GRP78 and Beclin-1 or by induction of Bak …

Stem Cell Technology For Age Related Macular Degeneration Intervention, Laura Valdez

Research Symposium

Retinal pigmented epithelium (RPE) cells are located between the choroid and photoreceptors within the eye and are essential to provide nutrients from blood to rods and cones, as well retinoids of the visual cycle. Vision loss and various ocular diseases are attributable to the degeneration or dysfunction of the RPE cells, leading to blindness.One of the major ocular problem from RPE dysfunction is macular degeneration. Age-related macular degeneration (AMD) can be frequently diagnosed in patients over the age of 60. In the early stages of AMD, some symptoms may not be noticeable but will lead to vision loss in both …

Standardization Of A Technique For Obtaining Dna From Footprints, Gibrán Galindo-Martinez, Karla Villarreal-Sotelo, Cynthia Marisol Vargas-Orozco, Ernesto Leal-Sotelo, Ignacio Hernandez-Rodriguez, José Francisco Flores-Gómez, Esperanza Milagros Garcia-Oropesa

Research Symposium

Currently our country has high numbers of missing persons, Tamaulipas being one of the states with the highest rate of disappearances. The identification of people has become more important thanks to the development of molecular techniques. However, the limitations are very high, because it is necessary to compare the genetic pattern of the disappeared with the parents. Therefore, the objective of this research is to standardize a genomic DNA extraction technique from contact surfaces for its subsequent implementation in the identification of disappeared, allowing the comparison of the genetic pattern with the disappeared itself. For this, genomic DNA extraction was …