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Circadian And Circatidal Rhythms Of Protein Abundance In The Intertidal Mussel Mytilus Californianus, Cory Elowe
Circadian And Circatidal Rhythms Of Protein Abundance In The Intertidal Mussel Mytilus Californianus, Cory Elowe
Master's Theses
The intertidal zone is a dynamic environment that fluctuates with the 12.4-h tidal and 24-h light/dark cycle to predictably alter food availability, temperature, air exposure, wave action, oxygen partial pressure, and osmotic conditions. Intertidal sessile bivalves exhibit behavioral or physiological changes to minimize the persistent challenges of fluctuating environmental conditions, such as adjusting gaping behavior and heart rate. At the cellular level, transcriptomic studies on mussels’ baseline circadian and circatidal rhythms have determined that the circadian rhythm is the dominant transcriptional rhythm. However, as proteins reflect the basic molecular phenotype of an organism and their abundance may differ greatly from …
The Proteomic Response Of Gill Tissue In Tidally And Subtidally-Acclimated California Mussels, Mytilus Californianus, To Acute Emersion-Induced Anoxia, Aubrie N. Fowler, Lars Tomanek
The Proteomic Response Of Gill Tissue In Tidally And Subtidally-Acclimated California Mussels, Mytilus Californianus, To Acute Emersion-Induced Anoxia, Aubrie N. Fowler, Lars Tomanek
Master's Theses
Intertidal mussels regularly experience emersion-induced anoxia, in contrast to normoxic conditions experienced during submersion. We therefore hypothesized that acclimation to a tidal rhythm, as opposed to a rhythm of constant submersion, preconditions the proteome of the California mussel, Mytilus californianus, to respond differently to emersion-induced anoxia. Following acclimation, mussels either continued to receive the acclimation conditions (control) or were exposed to 100% nitrogengas (anoxia) during aerial emersion. We collected gill tissue for subsequent analysis of protein abundance with 2D gel electrophoresis and protein identification with tandem mass spectrometry. Relative to subtidally-acclimated mussels, tidally-acclimated mussels showed a greater propensity to …