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Full-Text Articles in Life Sciences
Microscale Patchiness Of Bacterioplankton Assemblage Richness In Seawater, Richard A. Long, Farooq Azam
Microscale Patchiness Of Bacterioplankton Assemblage Richness In Seawater, Richard A. Long, Farooq Azam
Faculty Publications
We sought to test the hypothesis that bacterial species richness and composition vary at the millimeter scale in the marine pelagic environment, in response to the heterogeneous distribution of organic matter. To test this hypothesis, it was necessary to design and test a protocol to sample, lyse and analyze (by polymerase chain reaction/denaturing gradient gel electrophoresis [PCR-DGGE]) bacterioplankton species richness in a single microliter of seawater. Significant variation in bacterial species richness was found amongst 1 μl samples. Greater species richness was seen when seawater was enriched with diatom detritus. Examination of species richness at 2 mm intervals over a …
Variations In Bacterial Community Structure During A Dinoflagellate Bloom Analyzed By Dgge And 16s Rdna Sequencing, Laura B. Fandino, Lasse Riemann, Grieg F. Steward, Richard A. Long, Farooq Azam
Variations In Bacterial Community Structure During A Dinoflagellate Bloom Analyzed By Dgge And 16s Rdna Sequencing, Laura B. Fandino, Lasse Riemann, Grieg F. Steward, Richard A. Long, Farooq Azam
Faculty Publications
The relationship between bacterial 16S rRNA gene composition and carbon metabolism was analyzed during an intense dinoflagellate bloom off the Southern California coast during the spring of 1997. Bacterial numbers and rate processes, chlorophyll a, and the dissolved and particulate organic matter pools were measured during the bloom to provide a framework within which to assess bacterial community composition. Free bacteria were numerically dominant, generally comprising >90% of the total, and were responsible for >70% of bacterial production. Attached bacteria had higher cell-specific growth rates than free bacteria (range = 0.5 to 15.1 and 0.7 to 2.5 d-1 …