Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Life Sciences
Oncolytic Virus Therapy For The Treatment Of Metastatic Ovarian Cancer, Jessica Tong
Oncolytic Virus Therapy For The Treatment Of Metastatic Ovarian Cancer, Jessica Tong
Electronic Thesis and Dissertation Repository
The management of patients with epithelial ovarian cancer (EOC) faces two major challenges which standard treatments fail to effectively address: 1) Diffuse metastasis as a consequence of late stage diagnosis and 2) intra-tumoral heterogeneity, which fuels tumor evolution and drives the acquisition of chemotherapeutic resistance. In this thesis, we tested new therapeutic strategies using a 3-dimensional in vitro spheroid culture model that mimics key steps of epithelial ovarian cancer metastasis; and another model that mimics both temporal and cellular heterogeneity by establishing multiple cell lines from a single patient over the course of disease progression. Using these models, we investigated …
Exploring The Regulation And Function Of Epithelial-Mesenchymal Plasticity In Ovarian Cancer Spheroids, Samah Rafehi
Exploring The Regulation And Function Of Epithelial-Mesenchymal Plasticity In Ovarian Cancer Spheroids, Samah Rafehi
Electronic Thesis and Dissertation Repository
Epithelial-mesenchymal transition (EMT) serves as a key mechanism driving tumour cell migration, invasion, and metastasis in many carcinomas. Transforming growth factor-beta (TGFβ) signalling is implicated in several steps during cancer pathogenesis and acts as a classical inducer of EMT. Since epithelial ovarian cancer (EOC) cells have the potential to switch between epithelial and mesenchymal states during metastasis, we predicted that modulation of TGFβ signalling would significantly impact EMT and the malignant potential of EOC spheroid cells. Ovarian cancer patient ascites-derived cells naturally underwent an EMT response when aggregating into spheroids, and this was reversed upon spheroid re-attachment to a …