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Insights Into The Role Of Connexin26 And Connexin30 In Skin Health, Syndromic Disease, And Cutaneous Wound Healing, Eric R. Press
Insights Into The Role Of Connexin26 And Connexin30 In Skin Health, Syndromic Disease, And Cutaneous Wound Healing, Eric R. Press
Electronic Thesis and Dissertation Repository
Connexin26 (Cx26) and Cx30 facilitate gap junctional intercellular communication (GJIC) in the epidermis and are linked to several syndromic skin diseases. We investigated 5 disease- linked Cx26 mutants and demonstrated that the severity and extent of disease can be predicted from the gain- or loss-of function properties of each mutant, as well as the ability to induce cell death. We also used transgenic mice expressing S17F Cx26 (linked to keratitis-ichthyosis- deafness syndrome) or A88V Cx30 (linked to Clouston Syndrome) to investigate the pathophysiology these skin diseases. We demonstrated that S17F Cx26, but not A88V Cx30, promotes palmoplantar keratoderma by disrupting …
Identification Of A Novel Interaction Between Integrin-Linked Kinase And Beta-Arrestin 1, Hannah E. Murphy-Marshman
Identification Of A Novel Interaction Between Integrin-Linked Kinase And Beta-Arrestin 1, Hannah E. Murphy-Marshman
Electronic Thesis and Dissertation Repository
Integrin-linked kinase (ILK) is a ubiquitous scaffold protein essential for the development of front-rear polarity and directional migration of epidermal keratinocytes. β-arrestin 1 is another adaptor protein which has recently emerged as a key factor in modulating proliferation and migration of various cell types. Previous studies have demonstrated an association between β-arrestin 2 and ILK in cerebellar granule precursor cells. I have now identified a novel interaction between ILK and β-arrestin 1 in primary keratinocytes that occurs directly and without post-translational modifications. The N-terminal 67 residues of ILK and multiple regions in β-arrestin 1 are important for this interaction. This …
The Differential Effects Of Connexin26 And Connexin43 In Modulating Epidermal Health In Response To Uv Injury, Katanya C. Alaga
The Differential Effects Of Connexin26 And Connexin43 In Modulating Epidermal Health In Response To Uv Injury, Katanya C. Alaga
Electronic Thesis and Dissertation Repository
Using two disease-linked connexin mutant mice that possess significantly reduced connexin function (Cx43I130T/+ and Cx26K14-S17F/+) this investigation is the first to address the roles of Cx26 and Cx43 in modulating epidermal health in response to UV radiation. Viable Cx26K14-S17F/+ mice were successfully created in-house to express the mutant Cx26 S17F protein in keratinocytes and possess scaly skin, rapidly develop epidermal desquamation, and die soon after UV exposure. Conversely, Cx43I130T/+ mice did not possess any skin abnormalities before or after UV exposure. We also identified that primary murine melanocytes are not homocellularly coupled and do not …