Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 4 of 4
Full-Text Articles in Life Sciences
Production, Purification, And X-Ray Crystallography Of The Potra Domains Of Pstoc75, Gabriela Jordan Little
Production, Purification, And X-Ray Crystallography Of The Potra Domains Of Pstoc75, Gabriela Jordan Little
EURēCA: Exhibition of Undergraduate Research and Creative Achievement
Plastids arose via endosymbiosis when a cyanobacterium was engulfed by a primitive eukaryote. The cyanobacterium was enslaved by the eukaryote, eventually giving rise to a new organelle, termed the plastid. The transition from a free-living cyanobacterium to a cell-dependent organelle demonstrates how the chloroplast ancestor underwent many changes in its physiology and biological processes. The majority of the DNA belonging to the cyanobacterium was scavenged by the nucleus of the host cell. This led to higher fidelity of genetic duplication, due to the proofreading abilities of the DNA polymerase of the host cell. This left the cell with the problem …
Circadian Rhythmic Localization Of Tpa And Pai-1 In The Scn 2.2 Cell Culture May Provide Evidence For Determining The Mechanism Of Gating Photic Phase Shifts, Grayson T Hunley
Circadian Rhythmic Localization Of Tpa And Pai-1 In The Scn 2.2 Cell Culture May Provide Evidence For Determining The Mechanism Of Gating Photic Phase Shifts, Grayson T Hunley
EURēCA: Exhibition of Undergraduate Research and Creative Achievement
Mammalian circadian rhythms are controlled by a central pacemaker located in the suprachiasmatic nucleus (SCN) of the brain. The SCN exhibits endogenous rhythms in neuronal activity and entrains to external stimuli, particularly light. Interestingly, phase shifts in response to light only occur at night and the mechanisms gating phase shifting are not well characterized. Our lab demonstrated that the extracellular protease, tissue-type plasminogen activator (tPA) and its inhibitor, plasminogen activator inhibitor (PAI-1), help gate phase shifting. Total tPA and PAI-1 expression are rhythmic in mouse SCN. These proteins mediate different functions depending on their exact subcellular localization. Therefore, knowing where …
Real-Time Biomonitoring Of Cytotoxicity In 3d Autobioluminescent Human Tissue Culture Models, Haylie Rachel Lam
Real-Time Biomonitoring Of Cytotoxicity In 3d Autobioluminescent Human Tissue Culture Models, Haylie Rachel Lam
EURēCA: Exhibition of Undergraduate Research and Creative Achievement
Current optimal imaging technologies (e.g., fluorescence and firefly luciferase-based bioluminescence) require an external stimulation be applied prior to signal generation. To overcome this limitation, a ‘humanized’ bacterial luciferase reporter operon (lux) has been developed for expression in eukaryotic organisms, resulting in human cell lines capable of self-producing bioluminescent output without external stimulation. These cells can be programmed to continuously produce an autobioluminescent signal with declining intensity correlating with exposure to toxicants detrimental to cellular health or to auto-initiate bioluminescent production only in response to the detection of specific target agents. To evaluate the bioluminescent response to toxic compounds, constitutive autobioluminescent …
Plasticity In A Changing World: Phenotypic Variability In Boechera Stricta Across Space And Time, Caroline Daws
Plasticity In A Changing World: Phenotypic Variability In Boechera Stricta Across Space And Time, Caroline Daws
EURēCA: Exhibition of Undergraduate Research and Creative Achievement
While some plants are mobile and able to physically distribute to more suitable environments, other plants have limited dispersal ability and must find alternate ways to adjust to changing conditions in situ through adaptation or plasticity. Predicted changes in abiotic pressures, including drought stress and warmer temperatures, may influence the phenotypic expression of traits in plant populations through these mechanisms. In this study, we used a reciprocal transplant approach to investigate the temporal and spatial patterns of plasticity in a native perennial mustard, Boechera stricta, in the Rocky Mountains. Specifically, we explore how a temporal gradient that reflects drought stress …