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- Allergens (1)
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Articles 1 - 11 of 11
Full-Text Articles in Life Sciences
Functional Analysis Of The Fructooligosaccharide Utilization Operon In Lactobacillus Paracasei 1195, Yong Jun Goh, Jong-Hwa Lee, Robert W. Hutkins
Functional Analysis Of The Fructooligosaccharide Utilization Operon In Lactobacillus Paracasei 1195, Yong Jun Goh, Jong-Hwa Lee, Robert W. Hutkins
Department of Food Science and Technology: Faculty Publications
The fosABCDXE operon encodes components of a putative fructose/mannose phosphoenolpyruvate-dependent phosphotransferase system (PTS) and a β- fructosidase precursor (FosE) that are involved in the fructooligosaccharide (FOS) utilization pathway of Lactobacillus paracasei 1195. The presence of an N-terminal signal peptide sequence and a LPQAG cell wall anchor motif at the C-terminal region of the deduced FosE precursor amino acid sequence predicted that the enzyme is cell wall-associated, indicating that FOS may be hydrolyzed extracellularly. In this study, cell fractionation experiments demonstrated that the FOS hydrolysis activity was contained exclusively in the cell wall extract of L. paracasei previously grown on FOS. …
Crop Rotation And Soil Amendment Alters Sorghum Grain Quality, Nanga Mady Kaye, Stephen C. Mason, David S. Jackson, Tom D. Galusha
Crop Rotation And Soil Amendment Alters Sorghum Grain Quality, Nanga Mady Kaye, Stephen C. Mason, David S. Jackson, Tom D. Galusha
Department of Food Science and Technology: Faculty Publications
Soybean [Glycine max (L.) Merr.] rotation enhances grain sorghum [Sorghum bicolor (L.) Moench] yield, but infl uence on grain quality has not been measured. The objective was to determine the effect of cropping sequence (CS) and soil amendment (SA) on grain yield and quality. Sorghum grain yield and quality, soil NO3–N and water were measured in a rotation study in 2003 and 2004 on a Sharpsburg silty clay loam (fine, smectitic, mesic Typic Argiudoll). Cropping sequences were continuous sorghum, and sorghum rotated with non-nodulating and nodulating soybean. Soil amendments consisted of no amendment, manure (17–26 Mg …
Gene Family Encoding The Major Toxins Of Lethal Amanita Mushrooms, Heather E. Hallen-Adams, Hong Luo, John S. Scott-Craig, Jonathan D. Walton
Gene Family Encoding The Major Toxins Of Lethal Amanita Mushrooms, Heather E. Hallen-Adams, Hong Luo, John S. Scott-Craig, Jonathan D. Walton
Department of Food Science and Technology: Faculty Publications
Amatoxins, the lethal constituents of poisonous mushrooms in the genus Amanita, are bicyclic octapeptides. Two genes in A. bisporigera,AMA1 and PHA1, directly encode ∝-amanitin, an amatoxin, and the related bicyclic heptapeptide phallacidin, a phallotoxin, indicating that these compounds are synthesized on ribosomes and not by nonribosomal peptide synthetases. ∝-Amanitin and phallacidin are synthesized as proproteins of 35 and 34 amino acids, respectively, from which they are predicted to be cleaved by a prolyl oligopeptidase. AMA1 and PHA1 are present in other toxic species of Amanita section Phalloidae but are absent from nontoxic species in other sections. The …
Extraction And Characterization Of Starch From Alkaline Cooked Corn Masa, Wajira Srinanda Ratnayake, Andrew B. Wassinger, David S. Jackson
Extraction And Characterization Of Starch From Alkaline Cooked Corn Masa, Wajira Srinanda Ratnayake, Andrew B. Wassinger, David S. Jackson
Department of Food Science and Technology: Faculty Publications
Starch granules undergo structural and morphological changes during food processing unit operations as they interact with other food ingredients. This study was conducted to isolate and characterize starch granules from corn masa. A proteolytic enzyme, thermolysin, was effective in separating and isolating starch granules from endosperm proteins present in masa. The efficiency of starch extraction using thermolysin was 74% (w/w), and subsequent analyses showed that the isolated granules were free of contaminants. Starch samples were characterized using light microscopy, SEM, DSC, and XRD. Starch granules isolated from masa had undergone internal structural changes and some granules (≈40%) lost birefringence during …
Corroborative Study On Maize Quality, Dry-Milling And Wet-Milling Properties Of Selected Maize Hybrids, Kyung-Min Lee, Timothy J. Herrman, Lloyd Rooney, David S. Jackson, Jane Lingenfelser, Kent D. Rausch, John Mckinney, Chris Iiams, Linda Byrum, Charles R. Hurburgh Jr., Lawrence A. Johnson, Steven R. Fox
Corroborative Study On Maize Quality, Dry-Milling And Wet-Milling Properties Of Selected Maize Hybrids, Kyung-Min Lee, Timothy J. Herrman, Lloyd Rooney, David S. Jackson, Jane Lingenfelser, Kent D. Rausch, John Mckinney, Chris Iiams, Linda Byrum, Charles R. Hurburgh Jr., Lawrence A. Johnson, Steven R. Fox
Department of Food Science and Technology: Faculty Publications
A corroborative study was conducted on the maize quality properties of test weight, pycnometer density, tangential abrasive dehulling device (TADD), time-to-grind on the Stenvert hardness tester (SHT), 100-kernel weight, kernel size distribution, and proximate composition as well as maize dry- and wet-millability by six participating laboratories. Suggested operating procedures were given to compare their measurements and provide the variance structure within and between laboratories and hybrids. Partial correlation coefficient among maize quality properties varied among laboratories. The repeatability and reproducibility precision values were acceptably low for the physical quality tests, except for TADD and SHT time-to-grind measurements. The yields of …
Serum Testing Of Genetically Modified Soybeans With Special Emphasis On Potential Allergenicity Of The Heterologous Protein Cp4 Epsps, Michael Hoff, Dae-Yeul Son, Michaela Gubesch, Kangmo Ahn, Sang-Il Lee, Stefan Vieths, Richard E. Goodman, Barbara K. Ballmer-Weber, Gary A. Bannon
Serum Testing Of Genetically Modified Soybeans With Special Emphasis On Potential Allergenicity Of The Heterologous Protein Cp4 Epsps, Michael Hoff, Dae-Yeul Son, Michaela Gubesch, Kangmo Ahn, Sang-Il Lee, Stefan Vieths, Richard E. Goodman, Barbara K. Ballmer-Weber, Gary A. Bannon
Department of Food Science and Technology: Faculty Publications
Roundup Ready soy contains the CP4-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) protein. Serum IgE from two distinct populations of soy-allergic patients were recruited to determine their IgE-binding specificity. One population consisted of 10 adult patients from Europe, whose primary diagnosis was soy food allergy with some also having mite allergy. In addition, 6 primarily mite-allergic, 6 food-allergic (celery, carrot, milk, shrimp, walnut, and apple), and 5 non-allergic patients were tested. Another population consisted of 13 children from Korea, whose primary diagnosis was atopic dermatitis and secondarily soy and egg sensitization. In addition, 11 non-allergic patients were tested. Each patient population was extensively …
Genomic Deletion Marking An Emerging Subclone Of Francisella Tularensis Subsp. Holarctica In France And The Iberian Peninsula, M. P. Dempsey, M. Dobson, C. Zhang, M. Zhang, C. Lion, C. B. Gutiérrez-Martín, P. C. Iwen, P. D. Fey, M. E. Olson, D. Niemeyer, S. Francesconi, R. Crawford, M. Stanley, J. Rhodes, D. M. Wagner, A. J. Vogler, D. Birdsell, P. Keim, A. Johansson, S. H. Hinrichs, A. K. Benson
Genomic Deletion Marking An Emerging Subclone Of Francisella Tularensis Subsp. Holarctica In France And The Iberian Peninsula, M. P. Dempsey, M. Dobson, C. Zhang, M. Zhang, C. Lion, C. B. Gutiérrez-Martín, P. C. Iwen, P. D. Fey, M. E. Olson, D. Niemeyer, S. Francesconi, R. Crawford, M. Stanley, J. Rhodes, D. M. Wagner, A. J. Vogler, D. Birdsell, P. Keim, A. Johansson, S. H. Hinrichs, A. K. Benson
Department of Food Science and Technology: Faculty Publications
Francisella tularensis subsp. holarctica is widely disseminated in North America and the boreal and temperate regions of the Eurasian continent. Comparative genomic analyses identified a 1.59-kb genomic deletion specific to F. tularensis subsp. holarctica isolates from Spain and France. Phylogenetic analysis of strains carrying this deletion by multiple-locus variable-number tandem repeat analysis showed that the strains comprise a highly related set of genotypes, implying that these strains were recently introduced or recently emerged by clonal expansion in France and the Iberian Peninsula.
Lupine Allergy: Not Simply Cross-Reactivity With Peanut Or Soy, Kim A. B. M. Peeters, Julie A. Nordlee, André H. Penninks, Lingyun Chen, Richard E. Goodman, Carla A. F. M. Bruijnzeel-Koomen, Sue L. Hefle, Steve L. Taylor, André C. Knulst
Lupine Allergy: Not Simply Cross-Reactivity With Peanut Or Soy, Kim A. B. M. Peeters, Julie A. Nordlee, André H. Penninks, Lingyun Chen, Richard E. Goodman, Carla A. F. M. Bruijnzeel-Koomen, Sue L. Hefle, Steve L. Taylor, André C. Knulst
Department of Food Science and Technology: Faculty Publications
Background: Reports of lupine allergy are increasing as its use in food products increases. Lupine allergy might be the consequence of cross-reactivity after sensitization to peanut or other legumes or de novo sensitization. Lupine allergens have not been completely characterized. Objectives: We sought to identify allergens associated with lupine allergy, evaluate potential cross-reactivity with peanut, and determine eliciting doses (EDs) for lupine allergy by using double-blind, placebo-controlled food challenges. Methods: Six patients with a history of allergic reactions to lupine flour were evaluated by using skin prick tests, CAP tests, and double-blind, placebo-controlled food challenges. Three of these patients were …
Classification Of Dry-Milled Maize Grit Yield Groups Using Quadratic Discriminant Analysis And Decision Tree Algorithm, Kyung-Min Lee, Timothy J. Herrman, Scott R. Bean, David S. Jackson, Jane Lingenfelser
Classification Of Dry-Milled Maize Grit Yield Groups Using Quadratic Discriminant Analysis And Decision Tree Algorithm, Kyung-Min Lee, Timothy J. Herrman, Scott R. Bean, David S. Jackson, Jane Lingenfelser
Department of Food Science and Technology: Faculty Publications
A genetically and environmentally diverse collection of maize (Zea maize L.) samples was evaluated for physical properties and grit yield to help develop a standard set of criteria to identify grain best suited for drymilling. Application of principal component analysis (PCA) reduced a set of approximately 500 samples collected from six states to 154 maize hybrids. Selected maize hybrids were placed into seven groups according to their dry-milled grit yields. Regression analysis explained only 50% of the variability in dry-milling grit yield. Patterns of differences in the physical properties for the seven grit yield groups implied that the seven …
Identification Of Escherichia Coli O157:H7 Genomic Regions Conserved In Strains With A Genotype Associated With Human Infection, Marina Steele, Kim Ziebell, Yongxiang Zhang, Andrew K. Benson, Paulina Konczy, Roger Johnson, Victor Gannon
Identification Of Escherichia Coli O157:H7 Genomic Regions Conserved In Strains With A Genotype Associated With Human Infection, Marina Steele, Kim Ziebell, Yongxiang Zhang, Andrew K. Benson, Paulina Konczy, Roger Johnson, Victor Gannon
Department of Food Science and Technology: Faculty Publications
Beta-glucuronidase-negative, sorbitol-nonfermenting isolates of Shiga toxin-producing Escherichia coli O157 comprise part of a clone complex of related enterohemorrhagic E. coli isolates. High-resolution genotyping shows that the O157 populations have diverged into two different lineages that appear to have different ecologies. To identify genomic regions unique to the most common human-associated genotype, suppression subtractive hybridization was used to identify DNA sequences present in two clinical strains representing the human lineage I O157:H7 strains but absent from two bovine-derived lineage II strains. PCR assays were then used to test for the presence of these regions in 10 lineage I strains and 20 …
Genome Evolution In Major Escherichia Coli O157:H7 Lineages, Yongxiang Zhang, Chad Laing, Marina Steele, Kim Ziebell, Roger Johnson, Andrew K. Benson, Eduardo Taboada, Victor Gannon
Genome Evolution In Major Escherichia Coli O157:H7 Lineages, Yongxiang Zhang, Chad Laing, Marina Steele, Kim Ziebell, Roger Johnson, Andrew K. Benson, Eduardo Taboada, Victor Gannon
Department of Food Science and Technology: Faculty Publications
Background: Genetic analysis of Escherichia coli O157:H7 strains has shown divergence into two distinct lineages, lineages I and II, that appear to have distinct ecological characteristics, with lineage I strains more commonly associated with human disease. In this study, microarray-based comparative genomic hybridization (CGH) was used to identify genomic differences among 31 E. coli O157:H7 strains that belong to various phage types (PTs) and different lineage-specific polymorphism assay (LSPA) types.
Results: A total of 4,084 out of 6,057 ORFs were detected in all E. coli O157:H7 strains and 1,751 were variably present or absent. Based on this data, E. coli …