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Department of Agronomy and Horticulture: Faculty Publications

2017

Agrobacterium tumefaciens

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Full-Text Articles in Life Sciences

Molecular And Phenotypic Characterization Of Transgenic Wheat And Sorghum Events Expressing The Barley Alanine Aminotransferase, Pamela A. Peña, Truyen Quach, Shirley Sato, Zhengxiang Ge, Natalya Nersesian, Ismail M. Dweikat, Madhavan Soundararajan, Tom Clemente Jan 2017

Molecular And Phenotypic Characterization Of Transgenic Wheat And Sorghum Events Expressing The Barley Alanine Aminotransferase, Pamela A. Peña, Truyen Quach, Shirley Sato, Zhengxiang Ge, Natalya Nersesian, Ismail M. Dweikat, Madhavan Soundararajan, Tom Clemente

Department of Agronomy and Horticulture: Faculty Publications

Main conclusion — The expression of a barley alanine aminotransferase gene impacts agronomic outcomes in a C3 crop, wheat.

The use of nitrogen-based fertilizers has become one of the major agronomic inputs in crop production systems. Strategies to enhance nitrogen assimilation and flux in planta are being pursued through the introduction of novel genetic alleles. Here an Agrobacterium-mediated approach was employed to introduce the alanine aminotransferase from barley (Hordeum vulgare), HvAlaAT, into wheat (Triticum aestivum) and sorghum (Sorghum bicolor), regulated by either constitutive or root preferred promoter elements. Plants harboring the transgenic …