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The Effects Of Cold And Freezing Temperatures On The Blood Brain Barrier And Aquaporin 1, 4, And 9 Expression In Cope's Gray Treefrog (Hyla Chrysoscelis), Dalal Nouruldeen Felemban
The Effects Of Cold And Freezing Temperatures On The Blood Brain Barrier And Aquaporin 1, 4, And 9 Expression In Cope's Gray Treefrog (Hyla Chrysoscelis), Dalal Nouruldeen Felemban
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In sub-freezing temperatures, cerebrospinal fluid (CSF) and blood plasma within the brain of freeze-tolerant gray treefrogs, Hyla chrysoscelis, are likely to freeze. We hypothesized that this would challenge blood-brain barrier (BBB) integrity and volume regulation for cells in the brain. Our first hypothesis, tested as cerebral Evans blue permeability, was not supported; BBB integrity appeared uncompromised by cold. Our second hypothesis, tested as changing expression of aquaporin (AQP) proteins, was partially supported. Whole-brain expression (Western blot) of AQP1 and AQP4 were unchanged by cold conditions, but AQP9 expression increased in thawed animals. AQP1 was found (immunohistochemistry) in ependymal cells, endothelial …