Life Sciences Commons^™

A 100-M-Scale Modeling Study Of A Gale Event On The Lee Side Of A Long Narrow Mountain, Halie Xue, Jian Li, Tingting Qian, Hongping Gu

Plants, Soils, and Climate Faculty Publications

In this study, a gale event that occurred on the lee side of a long narrow mountain was investigated, together with the associated mountain flows, using a realistic-case large-eddy simulation (LES) that is based on the Weather Research and Forecasting Model. The mountain is located on the southeastern Tibetan Plateau, where approximately 58 gales occur annually, mostly in the afternoons during the winter season. Benefitting from realistic topography and high horizontal resolution as fine as 111 m, the LES can replicate features similar to the wind fields observed during the gale period. Investigation of the early morning wind structure over …

Green And Animal Manure Use In Organic Field Crop Systems, Patrick M. Carr, Michel A. Cavigelli, Heather Darby, Kathleen Delate, Jed O. Eberly, Heather K. Fryer, Greta G. Gramig, Joseph R. Heckman, Ellen B. Mallory, Jennifer R. Reeve, Erin M. Silva, David H. Suchoff, Alex L. Woodley

Plants, Soils, and Climate Faculty Publications

Dual‐use cover/green manure (CGM) crops and animal manure are used to supply nitrogen (N) and phosphorus (P) to organically grown field crops. A comprehensive review of previous research was conducted to identify how CGM crops and animal manure have been used to meet N and P needs of organic field crops, and to identify knowledge gaps to direct future research efforts. Results indicate that: (a) CGM crops are used to provide N to subsequent cash crops in rotations; (b) CGM‐supplied N generally can meet field crop needs in warm, humid regions but is insufficient for organic grain crops grown in …

Single-Molecule Real-Time Transcript Sequencing Identified Flowering Regulatory Genes In Crocus Sativus, Xiaodong Qian, Youping Sun, Guifen Zhou, Yumei Yuan, Jing Li, Huilian Huang, Limin Xu, Liqin Li

Plants, Soils, and Climate Faculty Publications

Background: Saffron crocus (Crocus sativus) is a valuable spice with medicinal uses in gynaecopathia and nervous system diseases. Identify flowering regulatory genes plays a vital role in increasing flower numbers, thereby resulting in high saffron yield.

Results: Two full length transcriptome gene sets of flowering and non-flowering saffron crocus were established separately using the single-molecule real-time (SMRT) sequencing method. A total of sixteen SMRT cells generated 22.85 GB data and 75,351 full-length saffron crocus unigenes on the PacBio RS II panel and further obtained 79,028 SSRs, 72,603 lncRNAs and 25,400 alternative splicing (AS) events. Using an Illumina RNA-seq platform, …

Salt Tolerance Of Sego Supremetm Plants, Asmita Paudel, Ji Jhong Chen, Youping Sun, Yuxiang Wang, Richard M. Anderson

Plants, Soils, and Climate Faculty Publications

Sego Supreme^TM is a designated plant breeding and introduction program at the Utah State University Botanical Center and the Center for Water Efficient Landscaping. This plant selection program introduces native and adapted plants to the arid West for aesthetic landscaping and water conservation. The plants are evaluated for characteristics such as color, flowering, ease of propagation, market demand, disease/pest resistance, and drought tolerance. However, salt tolerance has not been considered during the evaluation processes. Four Sego Supreme^TM plants [Aquilegia barnebyi (oil shale columbine), Clematis fruticosa (Mongolian gold clematis), Epilobium septentrionale (northern willowherb), and Tetraneuris acaulis var. arizonica …

Differences In Drought Tolerance Among Gisela® Cherry Rootstocks Determined Using Automated Weighing Lysimeters, Lance V. Stott, Brent Black, Bruce Bugbee

Plants, Soils, and Climate Faculty Publications

The Gisela® series of dwarfing rootstock are widely used because they enable high-density production, but they may be sensitive to drought. Drought tolerance may be associated with root-zone distribution and depth or with physiological adaptation to low water potential. Here we describe a novel technique for determining physiological tolerance to drought when root distribution is held constant. In two matching studies, we continuously measured transpiration of two groups of eight trees using a 16-container automated weighing lysimeter system in a greenhouse. With this system, Gisela® 3, 5, and 12 (G.3, G.5, and G.12) rootstocks were subjected to multiple, controlled drought …

Growth, Gas Exchange, And Mineral Nutrients Of Ornamental Grasses Irrigated With Saline Water, Yuxiang Wang, Youping Sun, Genhua Niu, Chaoyi Deng, Yi Wang, Jorge Gardea-Torresdey

Plants, Soils, and Climate Faculty Publications

Ornamental grasses are commonly used in urban landscapes in Utah and the Intermountain West of the United States. The relative salt tolerance of Eragrostis spectabilis (Pursh) Steud. (purple love grass), Miscanthus sinensis Andersson ‘Gracillimus’ (maiden grass), Panicum virgatum L. ‘Northwind’ (switchgrass), and Schizachyrium scoparium (Michx.) Nash (little bluestem) were evaluated in a greenhouse. Plants were irrigated with a nutrient solution at an electrical conductivity (EC) of 1.2 dS·m^–1 (control), or saline solution at an EC of 5.0 or 10.0 dS·m^–1. At harvest (65 days after the initiation of treatment), P. virgatum and S. scoparium exhibited no foliar …

Quantitative Trait Loci (Qtl) For Forage Traits In Intermediate Wheatgrass When Grown As Spaced-Plants Versus Monoculture And Polyculture Swards, John S. Mortenson, Blair L. Waldron, Steven R. Larson, Kevin B. Jensen, Lee R. Dehann, Michael D. Peel, Paul G. Johnson, J. Earl Creech

Plants, Soils, and Climate Faculty Publications

It has been hypothesized that the genetic control of forage traits, especially biomass, for grass plants growing as spaced-plants versus swards is different. Likewise, the genetic control of compatibility in grass–legume polyculture mixtures is assumed to be different than for forage production in a grass monoculture. However, these hypotheses are largely unvalidated, especially at the DNA level. This study used an intermediate wheatgrass mapping population to examine the effect of three competition environments (spaced-plants, polyculture, and monoculture) on classical quantitative genetic parameters and quantitative trait loci (QTL) identification for biomass, morphology, and forage nutritive value. Moderate to high heritable variation …

Controls And Adaptive Management Of Nitrification In Agricultural Soils, Jeanette M. Norton, Yang Ouyang

Plants, Soils, and Climate Faculty Publications

Agriculture is responsible for over half of the input of reactive nitrogen (N) to terrestrial systems; however improving N availability remains the primary management technique to increase crop yields in most regions. In the majority of agricultural soils, ammonium is rapidly converted to nitrate by nitrification, which increases the mobility of N through the soil matrix, strongly influencing N retention in the system. Decreasing nitrification through management is desirable to decrease N losses and increase N fertilizer use efficiency. We review the controlling factors on the rate and extent of nitrification in agricultural soils from temperate regions including substrate supply, …

Comparing The Salt Tolerance Of Three Landscape Plants Using A Near-Continuous Gradient Dosing System, Ji Jhong Chen, Yuxiang Wang, Asmita Paudel, Youping Sun

Plants, Soils, and Climate Faculty Publications

Screening salinity-tolerant plants is usually time intensive and only applicable to a limited number of salinity levels. A near-continuous gradient dosing (NCGD) system allows researchers to evaluate a large number of plants for salinity tolerance with multiple treatments, more flexibility, and reduced efforts of irrigation. Rose of sharon (Hibiscus syriacus), ninebark (Physocarpus opulifolius), and japanese spirea (Spiraea japonica) were irrigated using an NCGD system with eight electrical conductivity (EC) levels ranging from 0.9 to 6.5 dS·m^–1. At 11 weeks after irrigation was initiated, there were no significant differences among EC levels in …

A Drift-Free Decadal Climate Prediction System For The Community Earth System Model, Yoshimitsu Chikamoto, Axel Timmermann, Matthew J. Widlansky, Shaoqing Zhang, Magdalena A. Balmaseda

Plants, Soils, and Climate Faculty Publications

Performance of a newly developed decadal climate prediction system is examined using the low-resolution Community Earth System Model (CESM). To identify key sources of predictability and determine the role of upper and deeper ocean data assimilation, we first conduct a series of perfect model experiments. These experiments reveal the importance of upper ocean temperature and salinity assimilation in reducing sea surface temperature biases. However, to reduce biases in the sea surface height, data assimilation below 300 m in the ocean is necessary, in particular for high-latitude regions. The perfect model experiments clearly emphasize the key role of combined three-dimensional ocean …

Phototrophic N2 And Co2 Fixation Using A Rhodopseudomonas Palustris-H2 Mediated Electrochemical System With Infrared Photons, Mathangi Soundararajan, Rhesa Ledbetter, Paul Kusuma, Shuyang Zhen, Paul Ludden, Bruce Bugbee, Scott A. Ensign, Lance C. Seefeldt

Plants, Soils, and Climate Faculty Publications

A promising approach for the synthesis of high value reduced compounds is to couple bacteria to the cathode of an electrochemical cell, with delivery of electrons from the electrode driving reductive biosynthesis in the bacteria. Such systems have been used to reduce CO2 to acetate and other C-based compounds. Here, we report an electrosynthetic system that couples a diazotrophic, photoautotrophic bacterium, Rhodopseudomonas palustris TIE-1, to the cathode of an electrochemical cell through the mediator H2 that allows reductive capture of both CO2 and N2 with all of the energy coming from the electrode and infrared (IR) photons. R. palustris TIE-1 …

Consecutive Extreme Flooding And Heat Wave In Japan: Are They Becoming A Norm?, Shih-Yu Simon Wang, Hyungjun Kim, Dim Coumou, Jin-Ho Yoon, Lin Zhao, Robert R. Gillies

Plants, Soils, and Climate Faculty Publications

In July 2018, Japan experienced two contrasting, yet consecutive, extreme events: a devastating flood in early July followed by unprecedented heat waves a week later. Death tolls from these two extreme events combined exceeded 300, accompanying tremendous economic losses (BBC: July 24, 2018; AP: July 30, 2018). Meteorological analysis on these 2018 events quickly emerged (JMA-TCC, 2018; Kotsuki et al., 2019; Tsuguti et al., 2019), highlighting several compound factors: a strengthened subtropical anticyclone, a deepened synoptic trough, and Typhoon Prapiroon that collectively enhanced the Baiu rainband (the Japanese summer monsoon), fostering heavy precipitation. The comprehensive study of these events, conducted …

Trends And Controls On Water-Use Efficiency Of An Old-Growth Coniferous Forest In The Pacific Northwest, Yueyang Jiang, Christopher J. Still, Bharat Rastogi, Gerald F. M. Page, Sonia Wharton, Frederick C. Meinzer, Steve Voelker, John B. Kim

Plants, Soils, and Climate Faculty Publications

At the ecosystem scale, water-use efficiency (WUE) is defined broadly as the ratio of carbon assimilated to water evaporated by an ecosystem. WUE is an important aspect of carbon and water cycling and has been used to assess forest ecosystem responses to climate change and rising atmospheric CO₂ concentrations. This study investigates the influence of meteorological and radiation variables on forest WUE by analyzing an 18 year (1998–2015) half-hourly time series of carbon and water fluxes measured with the eddy covariance technique in an old-growth conifer forest in the Pacific Northwest, USA. Three different metrics of WUE exhibit an …

Fall Tillage Reduced Nutrient Loads From Liquid Manure Application During The Freezing Season, Melanie N. Stock, Francisco J. Arriaga, Peter A. Vadas, Laura Ward Good, Michael D. Casler, Krishnapuram G. Karthikeyan, Zachariah P. Zopp

Plants, Soils, and Climate Faculty Publications

Reducing agricultural runoff is important year round, particularly on landscapes that receive wintertime applications of manure. No-tillage systems are typically associated with reduced runoff loads during the growing season, but surface roughness from fall tillage may aid infiltration on frozen soils by providing surface depressional storage. The timing of winter manure applications may also affect runoff, depending on snow and soil frost conditions. Therefore, the objective of this study was to evaluate runoff and nutrient loads during the freezing season from combinations of tillage and manure application timings. Six management treatments were tested in south-central Wisconsin during the winters of …

Interactive Effects Of Water And Fertilizer On Yield, Soil Water And Nitrate Dynamics Of Young Apple Tree In Semiarid Region Of Northwest China, Hanmi Zhou, Xiaoli Niu, Hui Yan, Na Zhao, Fucang Zhang, Lifeng Wu, Dongxue Yin, Roger Kjelgren

Plants, Soils, and Climate Faculty Publications

Exploring the interactive effect of water and fertilizer on yield, soil water and nitrate dynamics of young apple tree is of great importance to improve the management of irrigation and fertilization in the apple-growing region of semiarid northwest China. A two-year pot experiment was conducted in a mobile rainproof shelter of the water-saving irrigation experimental station in Northwest A&F University, and the investigation evaluated the response of soil water and fertilizer migration, crop water productivity (CWP), irrigation water use efficiency (IWUE), partial factor productivity (PFP) of young apple tree to different water and fertilizer regimes (four levels of soil water: …

Data Mining Climate Variability As An Indicator Of U.S. Natural Gas, Jacob Stuivenvolt-Allen, Simon S.-Y. Wang

Plants, Soils, and Climate Faculty Publications

Anomalously cold winters with extreme storms strain natural gas (NG) markets due to heightened demand for heating and electricity generation. While extended weather forecasting has become an indicator for NG management, seasonal (2–3 month) prediction could mitigate the impact of extreme winters on the NG market for consumers and industry. Interrelated climate patterns of ocean and atmospheric circulation anomalies exhibit characteristics useful for developing effective seasonal outlooks of NG storage and consumption due to their influence on the persistence and intensity of extreme winter weather in North America. This study explores the connection between the Pacific-North American climate systems and …

Dynamics Of Measured And Simulated Dissolved Phosphorus In Runoff From Winter-Applied Dairy Manure, Peter A. Vadas, Melanie N. Stock, Francisco J. Arriaga, Laura Ward Good, Krishnapuram G. Karthikeyan, Zachariah P. Zopp

Plants, Soils, and Climate Faculty Publications

Agricultural P loss from fields is an issue due to water quality degradation. Better information is needed on the P loss in runoff from dairy manure applied in winter and the ability to reliably simulate P loss by computer models. We monitored P in runoff during two winters from chisel-tilled and no-till field plots that had liquid dairy manure applied in December or January. Runoff total P was dominated by nondissolved forms when soils were bare and unfrozen. Runoff from snow-covered, frozen soils had much less sediment and sediment-related P, and much more dissolved P. Transport of manure solids was …

Apospory And Diplospory In Diploid Boechera (Brassicaceae) May Facilitate Speciation By Recombination-Driven Apomixis-To-Sex Reversals, John G. Carman, Mayelyn Mateo De Arias, Lei Gao, Xinghua Zhao, Becky M. Kowallis, David A. Sherwood, Manoj K. Srivastava, Krishna K. Dwivedi, Bo J. Price, Landon Watts, Michael D. Windham

Plants, Soils, and Climate Faculty Publications

Apomixis (asexual seed formation) in angiosperms occurs either sporophytically, through adventitious embryony, or gametophytically, where an unreduced female gametophyte (embryo sac) forms and produces an unreduced egg that develops into an embryo parthenogenetically. Multiple types of gametophytic apomixis occur, and these are differentiated based on where and when the unreduced gametophyte forms, a process referred to as apomeiosis. Apomeiotic gametophytes form directly from ameiotic megasporocytes, as in Antennaria-type diplospory, from unreduced spores derived from 1st division meiotic restitutions, as in Taraxacum-type diplospory, or from cells of the ovule wall, as in Hieracium-type apospory. Multiple types of apomeiosis occasionally occur in …

Increased Variability Of Thailand's Chao Phraya River Peak Season Flow And Its Association With Enso Variability: Evidence From Tree Ring Δ18O, Chenxi Xu, Brendan M. Buckley, Parichart Promchote, S.-Y. Simon Wang, Nathsuda Pumijumnong, Wenling An, Masaki Sano, Takeshi Nakatsuka, Zhengtang Guo

Plants, Soils, and Climate Faculty Publications

We present a statistically robust reconstruction of Thailand's Chao Phraya River peak season streamflow (CPRPF) that spans the 202 years from 1804 to 2005 CE. Our reconstruction is based on tree ring δ¹⁸O series derived from three Pinus merkusii sites from Laos and Thailand. The regional δ¹⁸O index accounts for 57% of the observed variance of CPRPF. Spatial correlation and 21‐year running correlation analyses reveal that CPRPF is greatly influenced by regional precipitation variations associated with the El Niño–Southern Oscillation (ENSO). Periods of enhanced and reduced ENSO activity are associated with strong and weak ENSO‐streamflow correlation, …

Metrics For Understanding Large-Scale Controls Of Multivariate Temperature And Precipitation Variability, John P. O'Brien, Travis A. O'Brien, Christina M. Patricola, S.-Y. Simon Wang

Plants, Soils, and Climate Faculty Publications

Two or more spatio-temporally co-located meteorological/climatological extremes (co-occurring extremes) place far greater stress on human and ecological systems than any single extreme could. This was observed during the California drought of 2011–2015 where multiple years of negative precipitation anomalies occurred simultaneously with positive temperature anomalies resulting in California’s worst drought on observational record. The large-scale drivers which modulate the occurrence of extremes in two or more variables remains largely unexplored. Using California wintertime (November–April) temperature and precipitation as a case study, we apply a novel, nonparametric conditional probability distribution method that allows for evaluation of complex, multivariate, and nonlinear relationships …

Relative Salt Tolerance Of Seven Japanese Spirea Cultivars, Yuxiang Wang, Liqin Li, Youping Sun, Xin Dai

Plants, Soils, and Climate Faculty Publications

Spirea (Spiraea sp.) plants are commonly used in landscapes in Utah and the intermountain western United States. The relative salt tolerance of seven japanese spirea (Spiraea japonica) cultivars (Galen, Minspi, NCSX1, NCSX2, SMNSJMFP, Tracy, and Yan) were evaluated in a greenhouse. Plants were irrigated with a nutrient solution with an electrical conductivity (EC) of 1.2 dSmL1 (control) or saline solutions with an EC of 3.0 or 6.0 dSmL1 once per week for 8 weeks. At 8 weeks after the initiation of treatment, all japanese spirea cultivars irrigated with saline solution with an EC of 3.0 dSmL1 still exhibited good or …

Expression Of The High-Affinity K+ Transporter 1 (Pphkt1) Gene From Almond Rootstock ‘Nemaguard’ Improved Salt Tolerance Of Transgenic Arabidopsis, Amita Kaundal, Devinder Sandhu, Marco Duenas, Jorge F. S. Ferreira

Plants, Soils, and Climate Faculty Publications

Soil salinity affects plant growth and development, which directly impact yield. Plants deploy many mechanisms to cope with, or mitigate, salt stress. One of such mechanism is to control movement of ions from root to shoot by regulating the loading of Na+ in the transpiration stream. The high-affinity K+ transporter 1 (HKT1) is known to play a role in the removal of Na+ from the xylem and bring it back to the root. As almond is a salt-sensitive crop, the rootstock plays an important role in successful almond cultivation in salt-affected regions. We currently lack knowledge on the molecular mechanisms …

Expression Of The High-Affinity K+ Transporter 1 (Pphkt1) Gene From Almond Rootstock ‘Nemaguard’ Improved Salt Tolerance Of Transgenic Arabidopsis, Amita Kaundal, Devinder Sandhu, Marco Duenas, Jorge F. S. Ferreira

Plants, Soils, and Climate Faculty Publications

Soil salinity affects plant growth and development, which directly impact yield. Plants deploy many mechanisms to cope with, or mitigate, salt stress. One of such mechanism is to control movement of ions from root to shoot by regulating the loading of Na+ in the transpiration stream. The high-affinity K+ transporter 1 (HKT1) is known to play a role in the removal of Na+from the xylem and bring it back to the root. As almond is a salt-sensitive crop, the rootstock plays an important role in successful almond cultivation in salt-affected regions. We currently lack knowledge on the molecular mechanisms involved …

Ground, Proximal, And Satellite Remote Sensing Of Soil Moisture, Ebrahim Babaeian, Morteza Sadeghi, Scott B. Jones, Carsten Montzka, Harry Vereecken, Markus Tuller

Plants, Soils, and Climate Faculty Publications

Soil moisture (SM) is a key hydrologic state variable that is of significant importance for numerous Earth and environmental science applications that directly impact the global environment and human society. Potential applications include, but are not limited to, forecasting of weather and climate variability; prediction and monitoring of drought conditions; management and allocation of water resources; agricultural plant production and alleviation of famine; prevention of natural disasters such as wild fires, landslides, floods, and dust storms; or monitoring of ecosystem response to climate change. Because of the importance and wide‐ranging applicability of highly variable spatial and temporal SM information that …

Pantropical Climate Interactions, Wenju Cai, Lixin Wu, Matthieu Lengaigne, Tim Li, Shayne Mcgregor, Jong-Seong Kug, Jin-Yi Yu, Malte F. Stuecker, Agus Santoso, Xichen Li, Yoo-Geun Ham, Yoshimitsu Chikamoto, Benjamin Ng, Michael J. Mcphaden, Yan Du, Et Al.

Plants, Soils, and Climate Faculty Publications

The El Niño–Southern Oscillation (ENSO), which originates in the Pacific, is the strongest and most well-known mode of tropical climate variability. Its reach is global, and it can force climate variations of the tropical Atlantic and Indian Oceans by perturbing the global atmospheric circulation. Less appreciated is how the tropical Atlantic and Indian Oceans affect the Pacific. Especially noteworthy is the multidecadal Atlantic warming that began in the late 1990s, because recent research suggests that it has influenced Indo-Pacific climate, the character of the ENSO cycle, and the hiatus in global surface warming. Discovery of these pantropical interactions provides a …

Radiometric Method For Determining Canopy Stomatal Conductance In Controlled Environments, Oscar Monje, Bruce Bugbee

Plants, Soils, and Climate Faculty Publications

Canopy stomatal conductance is a key physiological factor controlling transpiration from plant canopies, but it is extremely difficult to determine in field environments. The objective of this study was to develop a radiometric method for calculating canopy stomatal conductance for two plant species—wheat and soybean from direct measurements of bulk surface conductance to water vapor and the canopy aerodynamic conductance in controlled-environment chambers. The chamber provides constant net radiation, temperature, humidity, and ventilation rate to the plant canopy. In this method, stepwise changes in chamber CO₂ alter canopy temperature, latent heat, and sensible heat fluxes simultaneously. Sensible heat and …

Genetic Loci Associated With Salt Tolerance In Advanced Breeding Populations Of Tetraploid Alfalfa Using Genome-Wide Association Studies, Xiang-Ping Liu, Charles Hawkins, Michael D. Peel, Long-Xi Yu

Plants, Soils, and Climate Faculty Publications

Many agricultural lands in the western United States consist of soil with high concentrations of salt, which is detrimental to alfalfa (Medicago sativa L.) growth and production, especially in the region where water resource is limited. Developing alfalfa varieties with salt tolerance is imperative for sustainable production under increasing soil salinity. In the present study, we used advanced alfalfa breeding populations and evaluated five traits related to salt tolerance including biomass dry weight (DW) and fresh weight (FW), plant height (PH), leaf relative water content (RWC), and stomatal conductance (SC) under control and salt stress. Stress susceptibility index (SSI) …

Intensification Of The Decadal Activity In Equatorial Rossby Waves And Linkage To Changing Tropical Circulation, Wan-Ling Tseng, Shih-Yu (Simon) Wang, Huang-Hsiung Hsu, Jonathan D.D. Meyer

Plants, Soils, and Climate Faculty Publications

Equatorial Rossby waves (ERWs) are manifest as westward-propagating, planetary-scale waves that feature a symmetric pair of pressure and zonal wind fields about the equator. ERWs can modulate tropical convective activity, especially in South Asia and the Maritime Continents, and represent an important mode of intraseasonal variability additional to the Madden-Julian Oscillation. Changes in the frequency and intensity of ERWs during the recent decades were investigated based on observations of tropospheric winds and tropical convection. Spectral analyses indicated that ERWs appear to have intensified especially in the upper troposphere; this is associated with increased convective activity located off the equator. The …

Detecting Soil Macrofauna Using Ground-Penetrating Radar, Melanie N. Stock, David J. Hart, Nicholas J. Balster

Plants, Soils, and Climate Faculty Publications

Fossorial amphibians spend up to ten months belowground, but research into this critical habitat has been impeded by a lack of noninvasive detection methods. Ground-penetrating radar (GPR), however, offers a promising tool because amphibians have theoretically strong electromagnetic (EM) contrasts relative to the soil matrix, and thus potentially high detectability. The objectives of this study were to (1) evaluate GPR by (2) experimentally-inducing three soil thermal regimes that promote stratification in the burrowing depths of 15 Eastern American Toads (Anaxyrus americanus americanus) during the winter of 2011–2012 in Madison, WI, USA. We calculated reflectability and established the unique …