Life Sciences Commons^™

The Roles Of Elevated Bcl-2 In Ovarian Cancer, Nicole Shree Anderson

USF Tampa Graduate Theses and Dissertations

Ovarian cancer (OC) is the second most common gynecologic cancer; however it is responsible for the most gynecologic cancer-related deaths. Apoptosis evasion is an important mechanism in OC tumorigenesis, and the prototypic anti-apoptotic protein, B-cell lymphoma 2 (Bcl-2), is often overexpressed in OC tumors. Gaining a better understanding of the mechanism(s) behind Bcl-2 overexpression and potential extra-anti-apoptotic functions of Bcl-2 could elucidate the importance of elevated Bcl-2 in OC. In the current study, I show through immunohistochemical analysis of normal, benign, and OC tissue sections, that both epithelial and stromal Bcl-2 expression decreases with OC progression. However, the number of …

Deglacial Abrupt Climate Change In The Atlantic Warm Pool: A Gulf Of Mexico Perspective, Carlie Williams, Benjamin P. Flower, David W. Hastings, Thomas P. Guilderson, Kelly A. Quinn, Ethan A. Goddard

Marine Science Faculty Publications

During the last deglaciation, Greenland ice core and North Atlantic sediment records exhibit multiple abrupt climate events including the Younger Dryas cold episode (12.9-11.7 ka). However, evidence for the presence of the Younger Dryas in the Gulf of Mexico (GOM) and the relationship between GOM sea surface temperature (SST) and high-latitude climate change is less clear. We present new Mg/Ca-SST records from two varieties of the planktonic foraminifer Globigerinoides ruber (white and pink) to assess northern GOM SST history from approximately 18.4-10.8 ka. Thirty-five accelerator mass spectrometry (AMS) C-14 dates from Orca Basin core MD02-2550 provide excellent age control and …

A Half-Century Of Changes In China's Lakes: Global Warming Or Human Influence?, Ronghua Ma, Hongtao Duan, Chuanmin Hu, Xuezhi Feng, Ainong Li, Weimin Ju, Jiahu Jiang, Guishan Yang

Marine Science Faculty Publications

Lake size is sensitive to both climate change and human activities, and therefore serves as an excellent indicator to assess environmental changes. Using a large volume of various datasets, we provide a first complete picture of changes in China's lakes between 1960s–1980s and 2005–2006. Dramatic changes are found in both lake number and lake size; of these, 243 lakes vanished mainly in the northern provinces (and autonomous regions) and also in some southern provinces while 60 new lakes appeared mainly on the Tibetan Plateau and neighboring provinces. Limited evidence suggested that these geographically unbalanced changes might be associated primarily with …

Ocean Mass From Grace And Glacial Isostatic Adjustment, D. Chambers, John Wahr, Mark E. Tamisea, R Steven Nerem

Marine Science Faculty Publications

We examine geoid rates and ocean mass corrections from two published global glacial isostatic adjustment (GIA) models, both of which have been used in previous studies to estimate ocean mass trends from Gravity Recovery and Climate Experiment (GRACE) satellite gravity data. These two models are different implementations of the same ice loading history and use similar mantle viscosity profiles. The model results are compared with each other and with geoid rates determined from GRACE during August 2002 to November 2009. When averaged over the global ocean, the two models have rates that differ by nearly 1 mm yr⁻¹ of …

Evaluation Of Immunogene Therapy Using A Plasmid Encoding Il-15 Delivered By Electroporation In A 3d Tumor Model And A Mouse Melanoma Model, Bernadette Marrero

USF Tampa Graduate Theses and Dissertations

Melanoma is an aggressive disease with few effective treatment options. Non-toxic, anti-tumor therapies and prophylactic approaches are currently being investigated to identify treatment options that will control and remove late-stage melanoma.

The overall goal of this project was to establish an effective delivery method for a plasmid encoding human interleukin (phIL-15) into mouse melanoma cells (B16.F10) using the gene transfer technique electroporation (EP)¹. The EP delivery phIL-15 was optimized using an in vitro 3D tumor model. The purpose was to translate these IL-15 delivery conditions into an in vivo mouse melanoma model to study IL-15 signal transduction and …

Characterization Of The Role Of Nicotine And Delta 9-Thc In Modulation Of Neuroinflammation, Jared C. Ehrhart

USF Tampa Graduate Theses and Dissertations

Neuroinflammation is a major driving force in the progression of neurodegenerative disorders. Nicotinic acetylcholine receptors, as well as cannabinoid CB2 receptors, have been shown to have strong anti-inflammatory properties when activated. These effects are shown, in vivo, to be a result of stimulation of α7 nAChRs and CB2 cannabinoid receptors. Microglia cells, an immune cell in the brain, are shown to express both of these receptor subtypes. The studies detailed herein, investigated the ability of two compounds, nicotine and Δ9-THC, in modulation of inflammatory processes. Stimulation of these receptors on microglia using nicotine and Δ9-THC blocked the activation of these …

Molecular Mechanism Of Agc Kinases In Human Malignant, Shaokun Shu

USF Tampa Graduate Theses and Dissertations

The maintenance of normal cell function and tissue homeostasis is dependent on the precise regulation of multiple signaling pathways that control cellular decisions to either proliferate, differentiate, arrest cell growth, or initiate programmed cell death (apoptosis). Cancer arises when clones of mutated cells escape this balance and proliferate inappropriately without compensatory apoptosis. Deregulated cell growth occurs as a result of perturbed signal transduction that modulates or alters cellular behavior or function to keep the critical balance between the rate of cell-cycle progression (cell division) and cell growth (cell mass) on one hand, and programmed cell death (apoptosis, autophagy) on the …

The Human In 3d: Advanced Morphometric Analysis Of High-Resolution Anatomically Accurate Computed Models, Summer J. Decker

USF Tampa Graduate Theses and Dissertations

Computed virtual models of anatomical structures are proving to be of increasing value in clinical medicine, education and research. With a variety of fields focused on craniofacial and pelvic anatomy there is a need for accurate anatomical models. Recent technological advancements in computer and medical imaging technologies have provided the tools necessary to develop three-dimensional (3D) functional models of human anatomy for use in medicine (surgical planning and education), forensics and engineering (computer-aided design (CAD) and finite element analysis).

Traditionally caliper methodologies are used in the quantitative analysis of human anatomy. In order for experts in anatomy and morphometrics to …

The Biogeochemistry Of Submerged Coastal Karst Features In West Central Florida, Keith Michael Garman

USF Tampa Graduate Theses and Dissertations

West Central Florida is a complex karst environment with numerous sinkholes, springs, and submerged cave systems. Many of these karst features are anchialine, located within the subterranean estuary where freshwater and saltwater mix. Water quality data and/or fauna data were obtained from twenty-one karst features and their associated cave systems. The anchialine karst environment of the study area has a wide range of habitats with measured salinities ranging from freshwater at <0.2 ppt to sulfidic, hypersaline water at 38.5 ppt and measured pH readings ranging from 6.39 in water impacted by sulfur oxidizing bacteria to 10.3 in an isolated room of a cave. Stygobitic crustaceans were identified in conduits extending beneath the Gulf of Mexico supporting the hypotheses that freshwater crustaceans could survive higher sea levels in freshwater conduits beneath saltwater. The fauna associated with the anchialine cave systems included Sabellidae and Polychaeta worms, hydroids, cnidarians and hydrobiid snails. Jewfish Sink, like other anaerobic marine basins that were submarine springs, has four zones: oxic zone, transition zone, upper anoxic zone and anoxic bottom water. The upper zones have seasonal water quality variations from winter cooling and sinking of surface water and changes in the microbial communities. Activity of sulfate reducing bacteria is carbon limited in the anoxic zones, where sulfate reduction is the major metabolic process, and primary production is phosphate limited in the oxic zones. Organic input from the Gulf of Mexico drives the bacterial anaerobic ecosystem, resulting in a “sulfide pump”, in which sulfide percolates upward removing oxygen from the overlying sediment.

Time Series Of Bio-Optical Properties In A Subtropical Gyre: Implications For The Evaluation Of Interannual Trends Of Biogeochemical Properties, Zhongping Lee, Shaoling Shang, Chuanmin Hu, Marlon Lewis, Robert Arnone, Yonghong Li, Bertrand Lubac

Marine Science Faculty Publications

With a validated Quasi‐Analytical Algorithm, an 11 year (1998–2008) monthly time series of the primary optical properties of waters in the center of the South Pacific gyre was developed from Sea‐viewing Wide Field‐of‐view Sensor (SeaWiFS) and Moderate Resolution Imaging Spectroradiometer (MODIS). Also derived are chlorophyll a (Chl a) concentrations with the operational empirical algorithms for SeaWiFS and MODIS. The optical properties include the absorption coefficient (at 443 nm) of phytoplankton (a_ph) and that of the combination of detritus and gelbstoff (a_dg). From these time series, we further derived their annual background (summer low) …

Evolution Of The Hammerhead Cephalofoil: Shape Change, Space Utilization, And Feeding Biomechanics In Hammerhead Sharks (Sphyrnidae), Kyle Reid Mara

USF Tampa Graduate Theses and Dissertations

The relationship between form and function is often used to elucidate the biological role of a structure. Hammerhead sharks offer a unique opportunity to study form and function through phylogeny. Because sphyrnid sharks display a range of cranial morphologies this group can be used to address questions about the evolution of cranial design and investigate the effects of changes in head morphology on feeding structures and bite force. Geometric morphometrics, volumetric analyses, morphological dissections, and phylogenetic analyses of the cephalofoil were used to gain insight into changes in cranial design through evolutionary history. External morphometrics and internal volumetric analyses indicated …

Discrimination Of Human And Non-Human Sources Of Pollution In Gulf Of Mexico Waters By Microbial Source Tracking Methods And The Investigation Of The Influence Of Environmental Factors On Escherichia Coli Survival, Asja Korajkic

USF Tampa Graduate Theses and Dissertations

Water quality worldwide is assessed by enumeration of fecal indicator bacteria (FIB) (fecal coliforms, Escherichia coli, and enterococci) intended to act as surrogates for human enteric pathogens. In environmental waters, this predictive relationship is confounded by many possible sources of FIB with varying implications for human health. Many physico-chemical and biological factors influence the fate of enteric pathogens and FIB in aquatic habitats, but are poorly understood, thus limiting our understanding of the usefulness of FIB as fecal pollution indicators.

These studies explored the field application of a “toolbox” approach to microbial source tracking (MST) intended to discriminate between human …

A Global Evaluation Of Ocean Bottom Pressure From Grace, Omct, And Steric-Corrected Altimetry, D. Chambers, Josh K. Willis

Marine Science Faculty Publications

Ocean bottom pressure (OBP) from the Gravity Recovery and Climate Experiment (GRACE) and the Ocean Model for Circulation and Tides (OMCT) are compared globally with OBP computed from altimetry corrected for steric variations from Argo floats from January 2005 to December 2007. Two methods of smoothing the GRACE data are examined. The first uses a standard Gaussian smoother with a radius of 300 km. The second method projects those smoothed maps onto empirical orthogonal functions derived from OMCT in a least squares estimation in order to produce maps that better agree with the physical processes embodied by the model. These …

Chk2 Is Negatively Regulated By Protein Phosphatase 2a, Alyson Freeman

USF Tampa Graduate Theses and Dissertations

Checkpoint kinase 2 (CHK2) is an effector kinase of the DNA damage response pathway and although its mechanism of activation has been well studied, the attenuation of its activity following DNA damage has not been explored. Here, we identify the B'α subunit of protein phosphatase 2A (PP2A), a major protein serine/threonine phosphatase of the cell, as a CHK2 binding partner and show that their interaction is modulated by DNA damage. B'α binds to the SQ/TQ cluster domain of CHK2, which is a target of ATM phosphorylation. CHK2 is able to bind to many B' subunits as well as the PP2A …

On The Recurrent Ulva Prolifera Blooms In The Yellow Sea And East China Sea, Chuanmin Hu, Daqiu Li, Changsheng Chen, Jianzhong Ge, Frank E. Muller-Karger, Junpeng Liu, Feng Yu, Ming-Xia He

Marine Science Faculty Publications

A massive bloom of the green macroalgae Ulva prolifera (previously known as Enteromorpha prolifera) occurred in June 2008 in the Yellow Sea (YS), resulting in perhaps the largest “green tide” event in history. Using a novel index (Floating Algae Index) and multiresolution remote sensing data from MODIS and Landsat, we show that U. prolifera patches appeared nearly every year between April and July 2000–2009 in the YS and/or East China Sea (ECS), which all originated from the nearshore Subei Bank. A finite volume numerical circulation model, driven by realistic forcing and boundary conditions, confirmed this finding. Analysis of meteorological/environmental …

Neuroprotection With Anesthetics In Two Models Of Cerebral Ischemia, Rafael Eduardo Chaparro Buitrago

USF Tampa Graduate Theses and Dissertations

Neuroprotection with anesthetics has been studied for many decades; important advances in this field have modified the way Anesthesiologists treat patients in the operating room. Animal models have played an important role in the study of ischemia in the operating room. Recent studies have demonstrated that the effect of anesthetics seems to be different in different animal models. We decided to evaluate anesthetics in a well-known model of cerebral ischemia and also in hypotensive models designed by us. We used a model of cerebral ischemia (MCAO) to test anesthetics neuroprotective effect in a two-week period. Then, we used a model …

Gey 4360 Mental Health Assessment Of Older Adults, Lisa Brown

Service-Learning Syllabi

No abstract provided.

Moderate Resolution Imaging Spectroradiometer (Modis) Observations Of Cyanobacteria Blooms In Taihu Lake, China, Chuanmin Hu, Zhongping Lee, Ronghua Ma, Kun Yu, Daqiu Li, Shaoling Shang

Marine Science Faculty Publications

A novel approach was used with data from the Moderate Resolution Imaging Spectroradiometer (MODIS) to characterize the intense blooms of cyanobacteria (primarily Microcystis aeruginosa) in Taihu Lake, China's third largest freshwater lake. The approach involves first deriving a floating algae index (FAI) based on the medium‐resolution (250 and 500 m) MODIS reflectance data at 645, 859, and 1240 nm after correction of the ozone/gaseous absorption and Rayleigh scattering effects and then objectively determining the FAI threshold value (−0.004) to separate the bloom and nonbloom waters. By definition, the term “bloom” or “floating algae” refers to bloom where cyanobacteria form …

Hydrological And Oceanic Effects On Polar Motion From Grace And Models, Shaunggen Jin, Don Chambers, Byron D. Tapley

Marine Science Faculty Publications

Terrestrial water storage (TWS) and ocean bottom pressure (OBP) are major contributors to the observed polar motion excitations, second only to atmospheric mass movement. However, quantitative assessment of the hydrological and oceanic effects on polar motion remains unclear because of the lack of global observations. In this paper, hydrological and oceanic mass excitations to polar motion are investigated using monthly TWS and OBP derived from the Gravity Recovery and Climate Experiment (GRACE) for January 2003 until December 2008. The results from this analysis are compared with hydrological model excitations from the European Center for Medium-Range Weather Forecasts (ECMWF) and oceanic …

Whole Genome Wide Expression Profiles Of Vitis Amurensis Grape Responding To Downy Mildew By Using Solexa Sequencing Technology, Jiao Wu, Yali Zhang, Huiqin Zhang, Hong Huang, Kevin M. Folta, Jiang Lu

School of Information Faculty Publications

Background: Downy mildew (DM), caused by pathogen Plasmopara viticola (PV) is the single most damaging disease of grapes (Vitis L.) worldwide. However, the mechanisms of the disease development in grapes are poorly understood. A method for estimating gene expression levels using Solexa sequencing of Type I restriction-endonuclease-generated cDNA fragments was used for deep sequencing the transcriptomes resulting from PV infected leaves of Vitis amurensis Rupr. cv. Zuoshan-1. Our goal is to identify genes that are involved in resistance to grape DM disease.

Results: Approximately 8.5 million (M) 21-nt cDNA tags were sequenced in the cDNA library derived from PV pathogen-infected …

Animal Models Of Alzheimer's Disease, Gemma Casadesus, Gary Arendash, Frank Laferla, Mike Mcdonald

Molecular Biosciences Faculty Publications

No abstract provided.

Comparing Models Of Evolution For Ordered And Disordered Proteins, Celeste J. Brown, Audra K. Johnson, Gary W. Daughdrill

Molecular Biosciences Faculty Publications

Most models of protein evolution are based upon proteins that form relatively rigid 3D structures. A significant fraction of proteins, the so-called disordered proteins, do not form rigid 3D structures and sample a broad conformational ensemble. Disordered proteins do not typically maintain long-range interactions, so the constraints on their evolution should be different than ordered proteins. To test this hypothesis, we developed and compared models of evolution for disordered and ordered proteins. Substitution matrices were constructed using the sequences of putative homologs for sets of experimentally characterized disordered and ordered proteins. Separate matrices, at three levels of sequence similarity ( …

Direct Observations Of Basin-Wide Acidification Of The North Pacific Ocean, Robert H. Byrne, Sabine Mecking, Richard A. Feely, Xuewu Liu

Marine Science Faculty Publications

Global ocean acidification is a prominent, inexorable change associated with rising levels of atmospheric CO₂. Here we present the first basin-wide direct observations of recently declining pH, along with estimates of anthropogenic and non-anthropogenic contributions to that signal. Along 152°W in the North Pacific Ocean (22–56°N), pH changes between 1991 and 2006 were essentially zero below about 800 m depth. However, in the upper 500 m, significant pH changes, as large as −0.06, were observed. Anthropogenic and non-anthropogenic contributions over the upper 800 m are estimated to be of similar magnitude. In the surface mixed layer (depths to …

West Florida Shelf: A Natural Laboratory For The Study Of Ocean Acidification, Pamela Hallock, Lisa L. Robbins, Rebekka Larson, Tanya Beck, Patrick Schwing, Michael Martínez-Colón, Brad Gooch

Marine Science Faculty Publications

Declining oceanic pH and carbonate-ion concentrations are well-known consequences of increased atmospheric and surface-ocean partial pressure of carbon dioxide (pCO₂). The possible subject of shifts in seawater carbonate chemistry on biocalcification and survival rates of marine organisms provides questions amenable to both experimental and field study (Kleypas and Langdon, 2006). To date, limited quantitative data exist with which to formalize and test hypotheses regarding such impacts, particularly in continental-shelf settings. The continental shelves of Florida provide an ideal natural laboratory in which to test latitudinal (and temperature and depth) shifts in habitat ranges of calcifying organisms. Both the …

An Intercomparison Of Gps Ro Retrievals With Colocated Analysis And In Situ Observations Within Tropical Cyclones, Henry R. Winterbottom, Qingnong Xiao

Marine Science Faculty Publications

Observations from four Global Position System (GPS) Radio Occultation (RO) missions: Global Positioning System/Meteorology, CHAallenging Minisatellite Payload, Satellite de Aplicaciones Cientificas-C, and Constellation Observing System for Meteorology, Ionosphere and Climate and Taiwan's FORMOsa SATellite Mission #3 (COSMIC/FORMOSAT-3) are collected within a 600 km radius and ±180 minute temporal window of all observed tropical cyclones (TCs) from 1995 to 2006 that were recorded in the global hurricane best-track reanalysis data set (Jarvinen et al. (1984); Davis et al. (1984)). A composite analysis of tropical cyclone radial mean temperature and water vapor profiles is carried out using the GPS RO retrievals which …

Transposases Are The Most Abundant, Most Ubiquitous Genes In Nature, Ramy Aziz, Mya Breitbart, Robert Edwards

Marine Science Faculty Publications

Genes, like organisms, struggle for existence, and the most successful genes persist and widely disseminate in nature. The unbiased determination of the most successful genes requires access to sequence data from a wide range of phylogenetic taxa and ecosystems, which has finally become achievable thanks to the deluge of genomic and metagenomic sequences. Here, we analyzed 10 million protein-encoding genes and gene tags in sequenced bacterial, archaeal, eukaryotic and viral genomes and metagenomes, and our analysis demonstrates that genes encoding transposases are the most prevalent genes in nature. The finding that these genes, classically considered as selfish genes, outnumber essential …

Poles Apart: The “Bipolar” Pteropod Species Limacina Helicina Is Genetically Distinct Between The Arctic And Antarctic Oceans, Brian Hunt, Jan Strugnell, Nina Bednarsek, Katrin Linse, R. John Nelson, Evgeny Pakhomov, Brad Seibel, Dirk Steinke, Laura Würzberg

Marine Science Faculty Publications

The shelled pteropod (sea butterfly) Limacina helicina is currently recognised as a species complex comprising two sub-species and at least five “forma”. However, at the species level it is considered to be bipolar, occurring in both the Arctic and Antarctic oceans. Due to its aragonite shell and polar distribution L. helicina is particularly vulnerable to ocean acidification. As a key indicator of the acidification process, and a major component of polar ecosystems, L. helicina has become a focus for acidification research. New observations that taxonomic groups may respond quite differently to acidification prompted us to reassess the taxonomic status of …

Identifying Thresholds For Ecosystem-Based Management, Jameal F. Samhouri, Phillip S. Levin, Cameron H. Ainsworth

Marine Science Faculty Publications

Background: One of the greatest obstacles to moving ecosystem-based management (EBM) from concept to practice is the lack of a systematic approach to defining ecosystem-level decision criteria, or reference points that trigger management action.

Methodology/Principal Findings: To assist resource managers and policymakers in developing EBM decision criteria, we introduce a quantitative, transferable method for identifying utility thresholds. A utility threshold is the level of human-induced pressure (e.g., pollution) at which small changes produce substantial improvements toward the EBM goal of protecting an ecosystem's structural (e.g., diversity) and functional (e.g., resilience) attributes. The analytical approach is based on the detection of …

The 13C Suess Effect In Scleractinian Corals Mirror Changes In The Anthropogenic Co2 Inventory Of The Surface Oceans, Peter K. Swart, Lisa Greer, Brad E. Rosenheim, Chris S. Moses, Amanda J. Waite, A. Winter, Richard E. Dodge, Kevin Helmle

Marine Science Faculty Publications

[1] New δ¹³C data are presented from 10 coral skeletons collected from Florida and elsewhere in the Caribbean (Dominica, Dominican Republic, Puerto Rico, and Belize). These corals range from 96 to 200 years in age and were collected between 1976 and 2002. The change in the δ¹³C of the skeletons from these corals between 1900 and 1990 has been compared with 27 other published coral records from the Atlantic, Pacific, and Indian Oceans. The new data presented here make possible, for the first time, a global comparison of rates of change in the δ¹³C …

Flow And Retreat Of The Late Quaternary Pine Island-Thwaites Palaeo-Ice Stream, West Antarctica, Alastair G. C. Graham, Robert D. Larter, Karsten Gohl, Julian A. Dowdeswell, Claus-Dieter Hillenbrand, James A. Smith, Jeffrey Evans, Gerhard Kuhn, Tara Deen

Marine Science Faculty Publications

[1] Multibeam swath bathymetry and sub-bottom profiler data are used to establish constraints on the flow and retreat history of a major palaeo-ice stream that carried the combined discharge from the parts of the West Antarctic Ice Sheet now occupied by the Pine Island and Thwaites glacier basins. Sets of highly elongated bedforms show that, at the last glacial maximum, the route of the Pine Island-Thwaites palaeo-ice stream arced north-northeast following a prominent cross-shelf trough. In this area, the grounding line advanced to within ∼68 km of, and probably reached, the shelf edge. Minimum ice thickness is estimated at 715 …