Life Sciences Commons^™

Investigating The Role Of Bs21 In Vancomycin-Resistant Staphylococcus Aureus, Daniel Floyd

Senior Honors Projects

Staphylococcus aureus is a Gram-positive pathogenic bacterium commonly known for being the causative organism of staph infections in clinical settings. In previous literature, it has been observed that mutations in certain genes in S. aureus can result in resistance to commonly used antibiotics. Specifically, mutations in the gene rpsU, encoding the ribosomal protein bS21, have been present in strains of S. aureus resistant to the antibiotic vancomycin. The aim of my project is to validate these findings by creating a strain of S. aureus without bS21 and performing an antibiotic assay to determine the level of resistance to vancomycin …

Multicopy Suppression Of Ribosomal Protein Deletion Mutants, Jacqualine Cerbone

Senior Honors Projects

To suppress the ribosome assembly defective phenotype of a ribosomal protein uS17- deficient mutant, various ribosomal proteins were used to act as multicopy suppressors. Ribosomal protein uS17 is a highly conserved component of the 30S (small) ribosomal subunit and is important for 30S subunit assembly. Mutants with a deletion of rpsQ (the gene encoding uS17) are viable but have a severe 30S subunit assembly defect and a temperature-sensitive (ts) phenotype. While fully assembled 30S subunits do form, incomplete ("20S") particles lacking several proteins accumulate. We hypothesized that increasing the intracellular concentration of one or more of the proteins missing from …

Ballasting System For An Autonomous Underwater Vehicle, Noura Rayes, Bahram Nassersharif, Zach Champney, Kyle Alessandro, Jason Mirandou

Senior Honors Projects

NOURA RAYES (Mechanical Engineering); Ballasting System for an Autonomous Underwater Vehicle Sponsor: Bahram Nassersharif (Mechanical Engineering)

Over the last year, our Mechanical Engineering Capstone team, Nautilus, has been working to design a ballasting system for various Autonomous Underwater Vehicles (AUVs) sponsored by Raytheon Technologies. Common AUV systems are used for a wide range of applications from environmental monitoring, oceanic exploration, to data collection. AUVs also have many technical advantages that make underwater tasks more efficient, cost-effective, and generally safer. Raytheon Technology, specifically, is looking to improve their existing ballasting mechanism for their AUVs to use for military defense. For this …

Describing The Effect Of Thin Layer Sediment Placement On Coastal Microbial Communities Using Foraminifera Diversity, Elizabeth B. Billings

Senior Honors Projects

Salt marshes are rich ecosystems that play an important role in aquatic food systems and prevent erosion while also providing essential habitats for wildlife. Climate change has resulted in rising sea levels and erosion of these coastal wetlands. Thin Layer Sediment Placement (TLP) is a restoration effort being implemented to mitigate the effects of climate change on coastal ecosystems. The TLP process raises elevation of the marsh and provides substrates for vegetative growth by spraying dredged sediment over existing marsh. The impacts of TLP on microbial populations is not widely understood, therefore metrics are required to study the effects of …

Generation Of A More Immunogenic Fh Vlp Platform To Support Covid-19 Vaccine Development, Jewel E. Voyer, Xinyuan Chen Phd.

Senior Honors Projects

The SARS-CoV-2 based pandemic has seen almost a million deaths in just the United States according to the CDC. This number is due to the increase of variants of COVID-19, therefore calling a demand in vaccine production. Vaccine adjuvants and carriers have been studied to increase the immunogenicity and safety of a variety of immunizations. Flagellin fused Hepatitis B core (FH) virus like particles (VLP) has been studied to have beneficial effects of TLR5, IL6 levels, and lack of body temperature rise in murine study. The FH platform also provides an improvement from its predecessors FljB alone which is a …

Assessment Of The University Of Rhode Island's Dining Halls And Student Perceptions Of On-Campus Dining, Maria Cherry

Senior Honors Projects

University dining halls are understudied food environments that affect student diet quality. This study (1) assessed dining hall offerings at the University of Rhode Island (URI) using the Partnership for a Healthier America’s Healthier Campus Initiative (PAHA) criteria for healthier campus food environments and (2) evaluated student perceptions of the dining hall through a campus-wide survey (n=165). We evaluated breakfast, lunch, and dinner using spring cycle menus (16 weeks in 2019 and 4 in 2021) from two full-service dining halls totaling 29 meals in 2019, and 84 meals in 2021 to determine adherence with PAHA criteria for availability of: 1) …

A Pathogen Outside Its Host: F. Tularensis Survival In Water, Aisling Macaraeg

Senior Honors Projects

Francisella tularensis is a Gram-negative pathogenic bacterium and is the causative agent for tularemia. While our understanding of F. tularensis survival in the environment is limited, it is clear they can persist in freshwater aquatic environments for long periods of time. Currently, there is a gap in knowledge with the understanding of the genes involved with the long-term survival of F. tularensis in an aquatic environment. In the laboratory setting, it has been found that lower temperatures seem to allow longer survival. The aim of this project is to identify laboratory conditions that allow F. tularensis to survive longterm in …

Ciliate Endosymbiont Imaging Methodology, Aidan Boving, Roxanne Beinart

Senior Honors Projects

Ciliates, a phylum of diverse microbial eukaryotes, are found in a wide range of environments, including anoxia. Anaerobic ciliates host intracellular methanogenic archaea – a unique type of symbiosis. Despite their importance, anaerobic ciliates and their symbiotic relationships remain understudied. Included in this is the ability to image and quantify the intracellular methanogenic symbionts in a precise, timely and replicable procedure. This project aimed to improve the current fluorescence microscopy methodology used for endosymbiont quantification. The proposed method is intended for use with the Opera Phenix, a high throughput spinning disk confocal microscope which can count large amounts of individual …

Temporal And Spatial Comparison Of Pseudo-Nitzschia Species Composition And Domoic Acid In Narragansett Bay, Rhode Island And The Northeast U.S. Shelf, Isabella Church, Bethany D. Jenkins

Senior Honors Projects

Diatoms are unicellular algae that make up a significant portion of phytoplankton biomass at the base of marine food webs. Narragansett Bay (NB), RI is home to several species of the diatom genus Pseudo-nitzschia (P-n), some of which are known to cause harmful algal blooms through the production of the potent neurotoxin domoic acid (DA). This toxin can lead to Amnesic Shellfish Poisoning in humans through the consumption of affected shellfish. Although the presence of P-n has been well established in NB for over 50 years, DA levels have only recently become a problem, with shellfish harvest closures …

Integration Of Medicinal Fungi Into The Heber W. Youngken Jr. Medicinal Garden, Anthony Makashov

Senior Honors Projects

The Heber W. Youngken Jr. Medicinal Garden is a one-of-a-kind resource that houses close to 300 medicinal plants and is filled to the brim with educational opportunities. For the College of Pharmacy, this garden has been the centerpiece of many classes taught here at the University of Rhode Island and has roots as a physical teaching space. While the garden contains a plethora of plant species, medicinal fungi have yet to have been included in the garden, despite their relevance as both sources of pharmaceutical drug discovery and as natural products with inherent physiological benefits themselves. The purpose of my …

The Effect Of Cortisol On Abdominal Fat, Zach Guerrieo

Senior Honors Projects

ZACHARY GUERRIEO (Biological Sciences)

The effect of cortisol on abdominal fat accumulation

Sponsor: Brietta Oaks (Nutrition)

Cortisol is the stress hormone of the body, it increases sugars in the bloodstream, enhances the brain's use of glucose, and increases the availability of substances to repair tissues. A healthy person has a daily slope of cortisol where it is high when they wake up and then decreases throughout the day. Cortisol, however, can be high throughout the day for people who live stressful or unhealthy lives. There have been conversations that high cortisol can cause fat gain specifically in the abdominal area. …

Political Ecology For Environmental And Life Sciences Maf-Saf 547, Joanna Burkhardt

Library Impact Statements

No abstract provided.

Wildlife Tracking Nrs 432, Michael Cerbo

Collection Development Reports and Documents

No abstract provided.

Wildlife Tracking Nrs 432x, Michael Cerbo

Collection Development Reports and Documents

No abstract provided.

Lee Isaac Chung, Minari (2020): Having An Amerikorean Life, Nagehan Uzuner

Markets, Globalization & Development Review

Minari by Lee Isaac Chung is a drama which chronicles the life of a Korean family who moves to the USA during 1980s in pursuit for a better life. The acculturation process is experienced differently by family members. Children are mostly bored with their new life in the rural area of Arkansas while their mother, Monica, is terrified of living in a mobile home which is made of a truck trailer in the middle of nowhere. Meanwhile, the grandmother joins the family from Korea to take care of the kids with a more positive approach dealing with their struggles. The …

Laboratory Research Skills, Joanna Burkhardt

Library Impact Statements

No abstract provided.

Laboratory Research Skills (Permanent), Joanna Burkhardt

Library Impact Statements

No abstract provided.

Program Bs In Nutrition, Julia Lovett

Library Impact Statements

No abstract provided.

Nutritional Data And Corresponding Performance Impacts Of Uri Student-Athletes, Abigael Fenbert

Senior Honors Projects

This research project investigates the diets of various sport athletes at the University of Rhode Island. The question that this project aims to answer is if our athletes are meeting recommended daily values nutritionally, and if not, why? Research continues to grow regarding how performance implications are tied to varying nutritional disparities for athletes. Various studies show malnourishment leads to performance decline, increased injury risk, and improper recovery. This is influenced by factors such as deficits in caloric intake, macronutrient and micronutrient availability, and dehydration. For college athletes, a lack of food availability and nutritional knowledge leaves them especially predisposed …

Swimmers’ Upper Trapezius Activation During Shoulder Abduction Compared To Non-Overhead Athletes, Kendra Graham

Senior Honors Projects

Overhead sports such as baseball, tennis, volleyball, softball and swimming are known to cause rotator cuff imbalances and injuries. These injuries are typically caused by the repeated use of “throwing motion,” where the humerus is externally rotated and shoulder abducted and elevated. Previous research has shown that the function of the upper trapezius muscle in overhead athletes is likely to have improper activation (timing, magnitude) during various shoulder motions and/or activities of daily living. Swimmers are especially susceptible to this improper activation due to the use of their upper limbs in a repeated throwing motion. The added resistance of the …

Analyzing The Impacts Of Loss Of The Fanca Protein On Chromatin State, Mileena Nguyen

Senior Honors Projects

No abstract provided.

Particle Tracking Reveals Pelagic Red Crabs As Indicators Of Climate-Driven Range Expansion In The California Current, Elizabeth Saraf

Senior Honors Projects

Since their first recorded occurrence in 1859, Pelagic Red Crabs (PRC; Pleuroncodes planipes) have experienced increased frequency of episodic mass stranding events in the Eastern Pacific Ocean. PRC’s are transported over 1,000 km northward of their central domain from the coast of central Baja California, Mexico. They have been reported by the thousands on the beaches of central and southern California. Their presence influences the surrounding ecosystem and commercial fisheries because they are nutritional prey items and large predators change their diet to incorporate PRC’s when they are available. Seasonal coastal currents and the California Undercurrent transport warmer waters …

Sharing Raw Data From Bacterial Genomes For Collaborative Research On The Amped Server At Uri, Deborah Adenekan

Senior Honors Projects

No abstract provided.

Research Grant Management Bes 510, Michael Cerbo

Library Impact Statements

No abstract provided.

Zoo And Aquarium Animal Welfare, Michael Cerbo

Library Impact Statements

No abstract provided.

The Association Between Type-2 Diabetes Pathophysiology & Exercise Adherence, Sydney Raymond

Senior Honors Projects

According to the American Diabetes Association, 1 in 3 Americans will be diagnosed with diabetes. While some of these individuals will be prescribed medications as part of their treatment, most will also be advised to begin an exercise program to assist with blood glucose control. Additionally, while regular exercise is associated with lower HbA1C and decreased insulin/medication dependence, it is estimated that only about half of those diagnosed with diabetes will adhere to their exercise plans. Social, psychological, and physiological factors all play roles in affecting ones ability to adhere to an exercise regiment, and individuals with Type 2 Diabetes …

The Journey To Reach Herd Immunity: The United State's Efforts Towards Controlling Sars-Cov-2, Natalina Mastroianni

Senior Honors Projects

COVID-19 is a respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which emerged in December of 2019 and has been the leading global health concern since. SARS-CoV-2 is a positive sense single-stranded RNA virus coated with viral spike proteins that has led to over 500,000 deaths in the United States and is approaching 3,000,000 deaths worldwide. As COVID-19 persists globally in such high numbers, the virus has been able to undergo mutations, leading to three prominent variants: the United Kingdom variant (B.1.1.7), the South Africa variant (B.1.351), and the Brazil variant (P.1). These variants are more transmissible, …

The Journey To Reach Herd Immunity: The United State's Efforts Towards Controlling Sars-Cov-2, Natalina Mastroianni

Senior Honors Projects

COVID-19 is a respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which emerged in December of 2019 and has been the leading global health concern since. SARS-CoV-2 is a positive sense single-stranded RNA virus coated with viral spike proteins that has led to over 500,000 deaths in the United States and is approaching 3,000,000 deaths worldwide. As COVID-19 persists globally in such high numbers, the virus has been able to undergo mutations, leading to three prominent variants: the United Kingdom variant (B.1.1.7), the South Africa variant (B.1.351), and the Brazil variant (P.1). These variants are more transmissible, …

Rhetorical Field Methods For Science Communication Bes 521, Michael Cerbo

Library Impact Statements

No abstract provided.

Parisitology Bio 331, Michael Cerbo

Library Impact Statements

No abstract provided.