Life Sciences Commons^™

U.S. Drought Monitor, December 31, 2013, Matthew Rosencrans

United States Agricultural Commodities in Drought Archive

Drought map of U.S. for December 31, 2013 (12/31/13) plus: U.S. crop areas experiencing drought (map), Approximate percentage of crop located in drought, by state (bar graph), Percent of crop area located in drought, past 52 weeks (line graph) for: Corn, Soybeans, Hay, Cattle, Winter wheat.

U.S. Drought Monitor, December 24, 2013, Matthew Rosencrans

United States Agricultural Commodities in Drought Archive

Drought map of U.S. for December 24, 2013 (12/24/13) plus: U.S. crop areas experiencing drought (map), Approximate percentage of crop located in drought, by state (bar graph), Percent of crop area located in drought, past 52 weeks (line graph) for: Hay, Cattle, Winter wheat.

Beef Cattle Body Temperature During Climatic Stress: A Genome-Wide Association Study, Jeremy T. Howard, Stephen D. Kachman, Warren M. Snelling, E. John Pollak, Daniel C. Ciobanu, Larry A. Kuehn, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Cattle are reared in diverse environments and collecting phenotypic body temperature (BT) measurements to characterize BT variation across diverse environments is difficult and expensive. To better understand the genetic basis of BT regulation, a genome-wide association study was conducted utilizing crossbred steers and heifers totaling 239 animals of unknown pedigree and breed fraction. During predicted extreme heat and cold stress events, hourly tympanic and vaginal BT devices were placed in steers and heifers, respect i v e l y. I n d i v i dua l s we r e genotyped with t h e BovineSNP50K_v2 assay and data …

U.S. Drought Monitor, December 17, 2013, Mark D. Svoboda

United States Agricultural Commodities in Drought Archive

Drought map of U.S. for December 17, 2013 (12/17/13) plus: U.S. crop areas experiencing drought (map), Approximate percentage of crop located in drought, by state (bar graph), Percent of crop area located in drought, past 52 weeks (line graph) for: Corn, Soybeans, Hay, Cattle, Winter wheat.

U.S. Drought Monitor, December 10, 2013, Michael J. Brewer

United States Agricultural Commodities in Drought Archive

Drought map of U.S. for December 10, 2013 (12/10/13) plus: U.S. crop areas experiencing drought (map), Approximate percentage of crop located in drought, by state (bar graph), Percent of crop area located in drought, past 52 weeks (line graph) for: Corn, Soybeans, Hay, Cattle, Winter wheat.

Making Chlamydomonas Reinhardtii A Better Model Organism: Tackling The Inefficiency Of Nuclear Transgene Expression And Improving Methods For The Generation And Characterization Of Insertional Mutant Libraries, Thomas M. Plucinak

Department of Biochemistry: Dissertations, Theses, and Student Research

The green algal species Chlamydomonas reinhardtii possesses many beneficial features that have made it a useful model organism for many decades. Many types of experimentation however are difficult to conduct with this organism due to the relative under-development of genetic tools available for use. Tasks such as transgene expression, overexpression of proteins of interest (POIs) or site specific genomic modification that are routine in other more facile microbial model organisms such as Escherichia coli and yeast are difficult to accomplish in C. reinhardtii. The second chapter of this thesis describes the development of a novel nuclear transgene expression system …

Studies On The Regulation Of Fgf21 Gene Expression By (R)-Α-Lipoic Acid: Mechanistic Insight Into The Lipid Lowering Properties Of A Dithiol Dietary Molecule, Xiaohua Yi

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Controlling blood lipids is a major public health challenge of our time. The pleiotropic hormone-like polypeptide fibroblast growth factor 21 (FGF21) was recently recognized as a potent modulator of lipid and glucose metabolism and a promising treatment strategy for obesity related metabolic disorders, including dyslipidemia. A cost effective and practical alternative to the administration of recombinant FGF21 is to stimulate endogenous FGF21 production through diet. Our research identified (R)-a-lipoic acid (LA), a naturally occurring enzyme cofactor and dietary molecule found in green leafy vegetable and red meat, as an inducer of FGF21 expression. LA stimulated FGF21 production, demonstrated by a …

Biotinylation Of The C-Myc Promoter Binding Protein Mbp-1 Decreases C-Myc And Cox-2 Expression In Mammary Carcinoma Mcf-7 Cells, Jie Zhou

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Biotin feeding in a Balb/c mouse model of breast cancer suggest that the tumor load is ~100% greater in biotin-deficient mice compared with biotin-sufficient controls, and that the increase is associated with metastasis rather than new tumors. The oncogenes c-Myc and COX-2 are implicated in cancer metastasis, and their expressions are repressed by binding of MBP-1 to the promoter. Mass spectrometry studies, designed to discover novel biotin-containing proteins, suggest that lysine residues K10 and K12 in the c-Myc promoter binding protein (MBP-1) contains covalently bound biotin (MBP-1bio). We hypothesized that biotinylation of MBP-1 increases the binding of MBP-1 to the …

U.S. Drought Monitor, December 3, 2013, Michael J. Brewer

United States Agricultural Commodities in Drought Archive

Drought map of U.S. for December 3, 2013 (12/3/13) plus: U.S. crop areas experiencing drought (map), Approximate percentage of crop located in drought, by state (bar graph), Percent of crop area located in drought, past 52 weeks (line graph) for: Corn, Soybeans, Hay, Cattle, Winter wheat.

Changes In Poa Annua Populations In Response To Herbicides And Plant Growth Regulators, Jesse Brown

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Poa annua (annual bluegrass; ABG) is an invasive weedy species in turfgrass. Herbicides and plant growth regulators (PGRs) are often used for ABG control, providing limited or inconsistent results. Identifying shifts in ABG populations in response to these treatments would be beneficial for understanding inconsistent control. Our research employed amplified fragment length polymorphic (AFLP) markers with the objective to determine if there are changes in genetic structure of ABG populations after multiple-year season-long control programs in three states. Annual bluegrass was sampled after the second or third year of seven different season-long ABG treatments consisting of herbicide or PGR applications. …

Transcriptional Regulation Of The Porcine Gnrh Receptor Gene By Glucocorticoids, Chanho Lee

Department of Animal Science: Dissertations, Theses, and Student Research

Binding of GnRH to its receptor (GnRHR) stimulates the synthesis and secretion of the gonadotropins, as well as up-regulation of GnRHR. Thus, the interaction between GnRH and GnRHR represents a central point for regulation of reproduction. Glucocorticoids alter reproduction by reducing GnRH responsiveness of gonadotropes within the anterior pituitary gland, potentially via transcriptional regulation of the GnRHR gene. Investigation into this mechanism, however, revealed that the murine GnRHR gene was stimulated by glucocorticoids. To determine the effect of glucocorticoids on porcine GnRHR gene expression, gonadotrope-derived αT3-1 cells were transiently transfected with a vector containing 5118 bp of 5’ flanking sequence …

Predicting Invasion Risk Of Non-Native Plants Using A Modified I-Rank Assessment, Justin B. Williams

School of Natural Resources: Dissertations, Theses, and Student Research

Biological invasions are a global problem responsible for native species declines worldwide. Understanding the invasion risk from non-native species is important in establishing management goals and making decisions for managing native ecosystems. Useful modeling methods for quantifying or predicting invasion risk should consider research needs, data availability, and operate at an appropriate scale. I evaluated risk assessment methods towards answering a specific research question; which plant species pose the greatest risk of becoming invasive or having the greatest negative impact in Nebraska? I selected the I-Rank assessment method, which consists of 20 questions grouped into four risk categories or Subranks: …

A Population Model For Walleye In Nebraska Irrigation Reservoirs, Robert A. Kill

School of Natural Resources: Dissertations, Theses, and Student Research

Understanding how and why fish population size changes between years is a central theme in fisheries ecology. Fishery agencies have limited time and financial resources, thus there is a need for a quantitative way to direct the limited time and financial resources so agencies can manage fisheries more efficiently. I developed a tool for fishery managers that synthesizes common population indices and evaluated the relative importance of those indices given varying uncertainty in age-0 walleye Sander vitreus survival. Under most circumstances, I determined that resources are best utilized in reducing age-0 survival uncertainty when understanding walleye population growth. I applied …

Determining Scaphirhynchus Sturgeon Population Demographics And Dynamics: Implications For Range-Wide Management, Recovery, And Conservation, Martin J. Hamel

School of Natural Resources: Dissertations, Theses, and Student Research

Sturgeons (Acipenseridae) have experienced world-wide declines as a result of anthropogenic effects such as over-harvest, habitat degradation, altered flow regimes, and pollution. Nearly all European and Asian sturgeon species have experienced population declines and have subsequently been classified as either threatened or endangered. North American sturgeons have experienced a similar plight in that all eight native sturgeon species are listed as endangered, threatened, or of special concern. Direct linkages between North American sturgeon declines and anthropogenic effects are difficult to assess due to scale considerations, fluctuating environmental conditions, difficulty in capture, and the interaction of all these effects. To recover, …

Anoxia Tolerance Of Forensically Important Calliphorids, Melissa M. Lein

School of Natural Resources: Dissertations, Theses, and Student Research

Forensically important blow flies, Diptera: Calliphoridae, are among the first organisms to colonize carrion. After eggs hatch, the larvae of most blow fly species feed in an aggregation or “mass.” While in this mass larvae may experience periods of no oxygen (anoxia), little oxygen (hypoxia), or normal oxygen (normoxia), but the tolerance of blow fly larvae to severe hypoxic conditions is not known. I tested the anoxia tolerance of four species of calliphorids (Calliphora vicina, Cochliomyia macellaria, Lucilia sericata, and Phormia regina), using third stage larvae across five temperatures. Experiments were conducted by exposing larvae to pure nitrogen …

Genetic Variability Of Spined Soldier Bugs (Hemiptera: Pentatomidae) Sampled From Distinct Field Sites And Laboratory Colonies In The United States, Fatima Mustafa, Muhammad Ullah, Kathleen M. Kneeland, Thomas A. Coudron, W. Wyatt Hoback, S. R. Skoda, Jaime Molina-Ochoa, John E. Foster

Department of Entomology: Faculty Publications

The spined soldier bug, Podisus maculiventris (Say), is an important biological control agent of agricultural and forest pests; and it preys on eggs and larvae of lepidopteran and coleopteran species. Genetic variability among field samples collected from Michigan, Mississippi, Missouri, Florida and established laboratory colonies was examined using Amplified Fragment Length Polymorphism-Polymerase Chain Reaction (AFLP-PCR). Four AFLP primer pairs generated a total of 340 molecular markers for evaluation. Results from Analysis of Molecular Variance showed that the majority of the genetic variation occurred within populations (individuals from each sample site). Nei’s method indicated reduced genetic diversity in laboratory populations compared …

Population Occurrence And Pathogen Prevalence Of Lone Star (Acari: Ixodidae) Ticks Collected From Southeast Nebraska, Amanda C. Maegli

Department of Entomology: Dissertations, Theses, and Student Research

Lone star tick, Amblyomma americanum (L.), has recently become established in Nebraska; therefore, local biology, ecology, and tick-borne disease risk are not known. Research was conducted to determine monthly questing activity, establishment, and pathogenic microorganisms associated with the lone star tick in Nebraska.

Lone star tick populations were collected from May through August, 2012 in six sites in southeast Nebraska using carbon dioxide (CO₂) traps. A total of 747 adults, 3,076 nymphs, and 1,289 larvae were collected. Total ticks collected and monthly activity were significantly different for each site.

A semi-randomized sample of 251 adult ticks were selected …

Loss Of Vascular Endothelial Growth Factor A (Vegfa) Isoforms In The Testes Of Male Mice Causes Subfertility, Reduces Sperm Numbers, And Alters Expression Of Genes That Regulate Undifferentiated Spermatogonia, Ningxia Lu, Kevin M. De Avila, Debra T. Clopton, William E. Pohlmeier, Vanessa M. Brauer, Renee M. Mcfee, John S. Weber, Napoleone Ferrara, David W. Silversides, Andrea S. Cupp

Department of Animal Science: Faculty Publications

Vascular endothelial growth factor A (VEGFA) isoform treatment has been demonstrated to alter spermatogonial stem cell homeostasis. Therefore, we generated pDmrt1-Cre;Vegfa^−/− (knockout, KO) mice by crossing pDmrt1-Cre mice to floxed Vegfa mice to test whether loss of all VEGFA isoforms in Sertoli and germ cells would impair spermatogenesis. When first mated, KO males took 14 days longer to get control females pregnant (P < .02) and tended to take longer for all subsequent parturition intervals (9 days; P < .07). Heterozygous males sired fewer pups per litter (P < .03) and after the first litter took 10 days longer (P < .05) to impregnate females, suggesting a more progressive loss of fertility. Reproductive organs were collected from 6-month-old male mice. There were fewer sperm per tubule in the corpus epididymides (P < .001) and fewer ZBTB16-stained undifferentiated spermatogonia (P < .003) in the testes of KO males. Testicular mRNA abundance for Bcl2 (P < .02), Bcl2:Bax (P < .02), Neurog3 (P < .007), and Ret was greater (P = .0005), tended to be greater for Sin3a and tended to be reduced for total Foxo1 (P < .07) in KO males. Immunofluorescence for CD31 and VE-Cadherin showed no differences in testis vasculature; however, CD31-positive staining was evident in undifferentiated spermatogonia only in KO testes. Therefore, loss of VEGFA isoforms in Sertoli and germ cells alters genes necessary for long-term maintenance of undifferentiated spermatogonia, ultimately reducing sperm numbers and resulting in subfertility.

Atf3 Expression In The Corpus Luteum: Possible Role In Luteal Regression, Dagan Mao, Xiaoying Hou, Heather Talbott, Robert A. Cushman, Andrea S. Cupp, John S. Davis

Department of Animal Science: Faculty Publications

The present study investigated the induction and possible role of activating transcription factor 3 (ATF3) in the corpus luteum. Postpubertal cattle were treated at midcycle with prostaglandin F2α(PGF) for 0–4 hours. Luteal tissue was processed for immunohistochemistry, in situ hybridization, and isolation of protein and RNA. Ovaries were also collected from midluteal phase and first-trimester pregnant cows. Luteal cells were prepared and sorted by centrifugal elutriation to obtain purified small (SLCs) and large luteal cells (LLCs). Real-time PCR and in situ hybridization showed that ATF3 mRNA increased within 1 hour of PGF treatment in vivo. Western blot and immunohistochemistry demonstrated …

American Society Of Parasitologists Newsletter, V. 35, No. 3, Winter 2013, Scott Lyell Gardner

American Society of Parasitologists: Newsletter

An issue of the American Society of Parasitologists' quarterly newsletter, also called the Journal of Parasitology Newsletter.

Effect Of The Insecticide Lambda-Cyhalothrin On Rice Spider Populations In Southern Brazil, Everton N. L. Rodrigues, Milton De S. Mendonça Jr., Leila L. Fritz, Elvis A. Heinrichs, Lídia Fiuza

Department of Entomology: Faculty Publications

The adverse effect that insecticides have on spider populations is of major concern since these predators are a component in the biological control of rice insect pest populations. This study was designed to determine the effect of the pyrethroid insecticide Lambda-cyhalothrin on spider populations in rice fields. Treatments consisted of plots treated once with the insecticide and untreated plots (control). Spiders were sampled four times per crop year over a two-year period in each of three sites in the state of Rio Grande dol Sul, Brazil. Treatments were replicated four times. Sweep net sampling was conducted to sample the araneofauna. …

Iptakalim Attenuates Self-Administration And Acquired Goal-Tracking Behavior Controlled By Nicotine, S. Charntikov, N. Swalve, Steven T. Pittenger, K. Fink, S. Schepers, G. C. Hadlock, A. E. Fleckenstein, G. Hu, Ming Li, Rick A. Bevins

Department of Psychology: Faculty Publications

Iptakalim is an ATP-sensitive potassium channel opener, as well as an a4b2-containing nicotinic acetylcholine receptor (nAChR) antagonist. Pretreatment with iptakalim diminishes nicotine-induced dopamine (DA) and glutamate release in the nucleus accumbens. This neuropharmacological profile suggests that iptakalim may be useful for treatment of nicotine dependence. Thus, we examined the effects of iptakalim in two preclinical models. First, the impact of iptakalim on the interoceptive stimulus effect of nicotine was evaluated by training rats in a discriminated goal-tracking task that included intermixed nicotine (0.4 mg/kg, SC) and saline sessions. Sucrose was intermittently presented in a responseindependent manner only on nicotine sessions. …

Using Focus Groups To Understand What New Residents Want And Need In A Community, Randolph Cantrell, Cheryl Burkhart-Kriesel, P. Thares, R. Vogt

Rural Futures Institute: Publications

No abstract provided.

Repeated Asenapine Treatment Produces A Sensitization Effect In Two Preclinical Tests Of Antipsychotic Activity, Rongyin Qin, Yingzhu Chen, Ming Li

Department of Psychology: Faculty Publications

Among several commonly used atypical antipsychotic drugs, olanzapine and risperidone cause a sensitization effect in the conditioned avoidance response (CAR) and phencyclidine (PCP)- induced hyperlocomotion paradigms – two well established animal tests of antipsychotic drugs, whereas clozapine causes a tolerance effect. Asenapine is a novel antipsychotic drug recently approved for the treatment of schizophrenia and manic disorders. It shares several receptor binding sites and behavioral features with other atypical antipsychotic drugs. However, it is not clear what type of repeated effect (sensitization or tolerance) asenapine would induce, and whether such an effect is transferrable to other atypicals. In this study, …

First Documented Record Of Hooded Oriole (Icterus Cucullatus) In Nebraska, Joseph Gubanyi

Nebraska Bird Review

During May 2013 a very cooperative Hooded Oriole (Icterus cucullatus) provided lots of excitement for Nebraska birders, becoming the first documented record for the species in Nebraska. The bird was first observed 25 May 2013 by Susie and Bill Daro at their residence in Garrison (Butler County). The Daros have several oriole feeders, and Baltimore and Orchard Orioles are common visitors. On the morning of May 25 they noted an unusual oriole visiting their feeders and, after checking the National Geographic Field Guide to the Birds of North America, concluded it was a Hooded Oriole. They found …

Correction [December 2013]

Nebraska Bird Review

The first Nebraska record of an Acorn Woodpecker was from Holt Co., 19–22 May 1996, not Cherry Co., as was reported on page 112 of the September 2013 issue of The Nebraska Bird Review.

Index To Volume 81

Nebraska Bird Review

Akers, D. J. 164
Albano, D. J. 164
Alberts, Byron 103, 113
Allen: Edward 4, 52, 103, 136; Jan 136
Arctos 168
Arnold, K. A. 31
Aubushon, Kathy 34
Austin, O. L. 92
Avocet, American 59, 94, 95, 109, 142, 171

. . .

Yantachka, Jen 52
Yellowlegs: Greater 10, 59, 94, 96, 109, 142, 171; Lesser 60, 94, 96, 110, 142, 171
Yellowthroat, Common 71, 97, 117, 156, 173
Young, Matt 53
Zimmerman: Jerald 34; Roy 53
Ziolkowski, D., Jr. 80
Zonotrichia leucophrys: leucophrys 158; oriantha 158

Bridgeport Fall Field Days, Janis Paseka

Nebraska Bird Review

The 2013 NOU Fall Field Days took place at the Prairie Winds Community Center in Bridgeport on Sept. 20–22. The meeting, organized by Kathy DeLara, drew a total of 50 attendees. On Friday evening Luke Hamilton led a group owling to the Bridgeport SRA and to Courthouse Rock. Field trip destinations on Saturday and on Sunday morning included Bridgeport SRA, Fleisbach WMA (aka Facus Springs), Crescent Lake NWR, Hackberry Road in Banner Co., the I-80 Exit I area in Kimball Co, Oliver Reservoir, Wildcat Hills SRA and Wind Springs Ranch. Trips were led by Kathy DeLara, Ann Duey, Cheryl Hamilton, …

Hayden, Tristram, And A Pigeon From “Nebraska”, Rick Wright

Nebraska Bird Review

A passenger pigeon skin in the World Museum in Liverpool, England, was collected during an expedition to Nebraska and Dakota led by Gouverneur K. Warren between 1855 and 1857 and later cataloged by geologist and naturalist Ferdinand V. Hayden. For a time it was in the collection of Henry Baker Tristram, a famous naturalist and a founder of the British Ornithologists’ Union. Passenger pigeons were once “quite abundant” along the Missouri River.

Subscription And Organization Information [December 2013]

Nebraska Bird Review

The Nebraska Bird Review is published quarterly by the Nebraska Ornithologists' Union, Inc., as its official journal, and is sent to members not in arrears of dues. Annual subscription rates (on a calendar-year basis only): $25 in the United States and $35 in all other countries, payable in advance. Single copies are $7 each, postpaid, in the United States and $9 elsewhere. Send orders for back issues to Anita Breckbill, NOU Librarian, c/o Music Library, WMB 30, University of Nebraska, Lincoln, NE 68588-0101.

Memberships in the NOU (on a calendar-year basis only): Active Household (one or more people) $25; Sustaining …