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Trehalose Uptake Through P2x7 Purinergic Channels Provides Dehydration Protection, Gloria D. Elliott, Jennifer L. Cusick, Xiang-Hong Liu, Michael A. Menze, Trudy Witt, Steven C. Hand, Mehmet Toner

Michael Menze

The tetra-anionic form of ATP (ATP4-) is known to induce monovalent and divalent ion fluxes in cells that express purinergic P2X7 receptors (Steinberg et al., 1987; Sung et al., 1985), and with sustained application of ATP it has been shown that dyes as large as 831 daltons can permeate the cell membrane (Steinberg et al, 1987). The current study explores the kinetics of loading α,α-trehalose (342 daltons) into ATP stimulated J774.A1 cells, which are known to express the purinergic P2X7 receptor (Steinberg et al., 1987). Cells that were incubated at 37 ̊C in a 50 mM phosphate buffer (pH 7.0) …