Life Sciences Commons^™

Anthropogenic Litter In Urban Waterways: An Analysis Of Litter, Urbanization, And The Emerging Role Of Citizen Science In Beargrass Creek, Louisville, Ky., Ella Swigler

Electronic Theses and Dissertations

This thesis addressed the ever growing presence and persistence of anthropogenic litter (AL) in urban waterways. AL has been studied in marine environments, but research gaps exist in riverine ecosystems. Most of the AL that reaches the Earth’s oceans is carried by urban rivers, so understanding the relationship between these waterways and AL is critical in effectively fighting AL accumulation, especially for legacy plastic pollutants. The study explored fifteen sites throughout Beargrass Creek and recorded the quantity and type of AL present throughout the summer and fall of 2023. The National Geographic Marine Debris Tracker App was employed to successfully …

An Investigation Into The Structural Features That Control Factor Xiii Stability And Substrate Specificity., Rameesa Darul Amne Syed Mohammed

Electronic Theses and Dissertations

Factor XIII (FXIII) is a transglutaminase enzyme with multiple physiological roles that is found in plasma and cells of bone marrow origin. The catalytic A subunit (FXIII-A) is made of an N-terminal activation peptide (AP), β-sandwich, catalytic core, and two β-barrel domains. FXIII crosslinks the sidechains of glutamine (Q) and lysine (K) residues across plasmatic and cellular substrates. Because of its involvement in clot stabilization and determining fibrin clot size, FXIII-A is regarded as a target for developing new anticoagulants with minimal bleeding risks. However, the FXIII-A structural features that control its stability and substrate specificity largely remain unknown. Plasma …

Do American Bison Practice Democracy? Group Consensus Decision Making Drives Bison Herd Movements And Cohesion., Matthew Sapien

Electronic Theses and Dissertations

The American bison is an iconic mammal that lives in large social herds and is considered a threatened species. Little is known about how bison make decisions as a group, whether through despotic individuals or group consensus. In this dissertation, I researched the mechanisms bison use to make herd movement decisions in 3 herds in the United States. In chapter one I examined the number of bison that participated in voting events for herd movement decisions and whether the final movement decision matched the direction with the most votes. Bison herd movements did match the direction with the most adult …

Relationships Between Fitness Levels, Physical Activity Participation, Hemoglobin A1c Levels, And Motor Proficiency In Adolescent Patients With Type 1 Diabetes., Caroline E. Frye

Electronic Theses and Dissertations

Physical activity is a critical component of a healthy lifestyle and can lead to improved fitness levels and blood glucose management, as well as decreased HbA1c levels. PURPOSE: The purpose of this study was to determine if there are relationships between cardiorespiratory fitness, muscular strength, muscular endurance, flexibility, body composition, physical activity participation, hemoglobin A1C levels, and motor proficiency in adolescent patients with T1D. METHODS: Pediatric patients with a diagnosis of T1D enrolled in a sports medicine program were invited to participate in this study. Retrospective clinical chart reviews were conducted to determine participant’s sociodemographic and anthropometric measures and most …

Multithreaded Applications On The Heterogeneous Research Computing Environment., Sungbo Jung

Electronic Theses and Dissertations

Bioinformatics is a domain that has experienced rapid research growth in recent years, as evidenced by the increasing number of articles in biomedical databases such as PubMed, which adds over a million publications every year. However, this also poses a challenge for researchers who need to find relevant citations for their work. Therefore, developing efficient indexing and searching methods for text data is crucial for Bioinformatics. One key technique for information retrieval is document inversion, which involves creating an inverted index to enable efficient searching through vast collections of text or documents. This Ph.D. research aims to design the research …

Dendrochronological Analysis Of The Duncan Tavern, Paris, Kentucky, Usa, Delaney Ballard, Maegen Rochner

Undergraduate Research Events

Duncan Tavern is a historical structure located at 323 High Street in Paris, Kentucky in Bourbon County. The structure currently serves as the headquarters for the Kentucky Society of the Daughters of the American Revolution (KSDAR). KSDAR maintains that Duncan Tavern was originally built in the mid-1790s; the land was purchased in 1792 by Joseph Duncan, a civilian armorer in the Revolutionary War. Although Duncan Tavern has been listed on the National Register of Historical Places since 1973, a dendrochronological study of this structure was requested to quantitatively examine the legitimacy of archival and anecdotal claims about the construction history. …

Testing Functional Homology Of The Lga2 And Rga2 Genes Of Sporisorium Reilianum In Ustilago Maydis, Joshua Thomas

College of Arts & Sciences Senior Honors Theses

Ustilago maydis, a basidiomycete fungus, infects corn and displays dimorphism, transitioning between nonpathogenic and pathogenic stages. Its life cycle involves mating of haploids with different alleles, resulting in the formation of galls/tumors filled with teliospores. The a1 and a2 alleles regulate pheromone recognition and mating. The lga2 and rga2 genes in the a2 allele are crucial for uniparental mitochondrial inheritance. Sporisorium reilianum, a related fungus, has homologs of lga2 and rga2 with U. maydis. To test the functional homology of the S. reilianum genes, protoplasts of U. maydis strains deleted for lga2 or lga2 and rga2 were transformed …

Sdf-1Α Mediates Primary Tumor Escape In Glioblastoma Through Activation Of Mesenchymal Transitions., Charles T. Froman-Glover

College of Arts & Sciences Senior Honors Theses

Glioblastoma (GBM), a highly aggressive primary brain tumor originating in glial cells, poses a significant challenge due to its rapid growth and invasive nature within healthy brain tissue.

Current treatments involve surgical resection, chemotherapy, and radiation. These treatments alone are not enough to cure this disease, and a better understanding the mechanics of the tumor micro-environment is imperative to furthering the field of cancer research. This research focuses on understanding the tumor microenvironment's impact, specifically investigating the role of stromal cell-derived factor 1 (SDF-1) mechanics on GBM aggressiveness. SDF-1 is known to facilitate disease progression by facilitating chemotaxis toward the …

The Anti-Diabetic Drug Metformin Disrupts Feeding And Sleeping Behaviors In Drosophila Melanogaster., Lucas Fitzgerald

College of Arts & Sciences Senior Honors Theses

Dimethylbiguanide, also known as metformin, is the single most prescribed oral treatment for non-insulin dependent diabetes mellitus, or type 2 diabetes, in Western countries. The primary mechanism of action that metformin acts through is the activation of AMP kinase, an important regulator of energy homeostasis. While the anti-diabetic effects of metformin are well documented, its effects on feeding and sleeping behaviors are not well characterized. Using the model organism Drosophila melanogaster, the mean daily quantity of food consumed was measured and compared between groups treated with several dosages of metformin. Feeding interactions such as meal frequency and length were …

Assessing The Role Of Stim1 And Stim2 In Microglial Response To Spinal Cord Injury., Sarayu Alli

College of Arts & Sciences Senior Honors Theses

Microglia, immune cells of the central nervous system, constantly survey their environment for damage. Once damage is detected, they are activated into become reactive, resulting in morphological changes, such as larger cell bodies, and functional changes, such as releasing inflammatory mediators. The switch from surveying to reactive occurs because the microglia received signals that sense danger in the environment. The release of Ca2+ from intracellular stores is involved in store-operated calcium entry (SOCE), a signaling pathway. Recent studies have shown that calcium release from the intracellular stores is a factor of microglial activation. SOCE is specific to monitoring low calcium …

Exploring Resilience With Neuroimaging: Moderators Of The Impacts Of Childhood Traumatic Stress On Fear Processing., Karisa June Hunt

Electronic Theses and Dissertations

Childhood trauma is an alarming public health crisis, and the field of trauma research is relatively underdeveloped given population rates of childhood trauma and post-trauma pathology. Even less studied than the impacts of trauma are the impacts of resilience, and the protective factors that foster it. It is widely acknowledged that an investigation of trauma is incomplete without an investigation of its impacts on fear processing. Understanding the neural underpinnings of resilience to childhood trauma during fear conditioning is vital to the development of therapeutic interventions able to moderate its devastating impacts. The present study investigated the complex neurobiological interplay …

Investigation Of Avaren-Fc's Therapeutic Potential Against Ovarian Cancer., Katarina Lee Mayer

Electronic Theses and Dissertations

This thesis describes the investigation of Avaren-Fc (AvFc), a novel lectin-based therapeutic fusion protein consisting of the recombinant Avaren lectin and the Fc region of human IgG1, against ovarian cancer. AvFc possesses selectivity toward tumor associated N-linked high-mannose-type glycans. The present study demonstrates AvFc’s in vitro activity against OVCA cell lines via antibody-dependent cell-mediated cytotoxicity and its ability to extend the survival of mice challenged with murine ID8 OVCA cells after repeated systemic administration. Furthermore, AvFc treatment in this model was found to increase the presence of peritoneal leukocytes, suggesting a shift toward an improved antitumor immune response. Another key …

Inhibition Of Pro-Inflammatory Lipid Mediators By Yersinia Pestis., Amanda Brady

Electronic Theses and Dissertations

Yersinia pestis causes the human disease known as plague. A key manifestation of plague is a delayed inflammatory response. Because this delay in inflammation is required for virulence, I was interested in defining the molecular mechanisms used by Y. pestis to evade immune recognition. Eicosanoids are produced early during infection and necessary to initiate a rapid inflammatory response. Despite the importance of these lipids in mediating inflammation, the role of eicosanoids during plague has not been previously investigated. Using an intranasal mouse model infection, I determined the kinetics of eicosanoid synthesis during pneumonic plague. I further demonstrated that LTB_{4 …}

Assessing Sustainability Within The Brewing Industry: A Literature Review And Benchmarking Analysis., Elijah Thomas Miller

Electronic Theses and Dissertations

The goals of this study were twofold: to further examine sustainability within the brewing industry through a literature analysis across four academic journals, and a benchmarking analysis of a Louisville, Kentucky brewery’s resource consumption and emissions production. Publications related to the brewing industry were examined for the presence of sustainability keywords: “efficiency, sustainability, carbon footprint, climate change and greenhouse gas”. Benchmarking examined the local brewery’s primary production facility to determine their performance compared to other surveyed breweries pursuing sustainability across the country. Literature examination found noticeable discrepancies between the appearance of sustainability topics within article abstracts and their discussion within …

Effects Of Sex, Cadmium, And High-Fat-Diet On Accumulation Of Essential Metals In Mouse Kidneys., Justin Y. Xu, Adam Gaweda, Michelle Barati, Michael Merchant, Jun Cai, Lu Cai, Madhavi Rane

Undergraduate Research Events

Effects of cadmium and obesity on accumulation of essential metals in mouse kidney were evaluated. Starting in utero and continuing until 24 weeks postweaning, male/female mice were exposed to cadmium (0, 0.5, or 5 ppm) and were fed on Normal Diet (ND)/High-Fat Diet (HFD) post weaning. ICP-MS analysis was performed to evaluate cadmium and essential metals absorbed in the kidneys of aforementioned mice. Metals data obtained was analyzed using MANOVA with multiple comparisons (Bonferroni correction with p

Old Vs. New: An Experiment On The Efficacy Of Complexity-Focused Reproductive Biology Lectures For Undergraduate Biology Students, Ive N. Muse, Shraddha Patel, Linda Fuselier

Undergraduate Research Events

Undergraduate reproductive biology courses are traditionally taught in a reductive manner, prioritizing a binary understanding of sex which oversimplifies reproductive behaviors and reproductive system development across diverse taxa. Although this binary approach to lessons on reproduction is intended to benefit students in learning foundational human reproductive concepts, it does not teach the most recent research in reproductive biology, which identifies reproductive variation as the norm. We designed and implemented a lesson on reproductive biology informed by the most current and accurate research and compared it to a traditionally taught biology lecture for first year majors. Students who received the experimental …

Impact Of Sodium Benzoate And A High Fat Diet On Kidney Of Mice., Anna Lipinski

College of Arts & Sciences Senior Honors Theses

Sodium Benzoate (SB) is a commonly utilized food preservative with potential therapeutic uses for neurological conditions such as Alzheimer’s disease and hepatic encephalopathy. Consumption of SB is generally regarded as safe (GRAS), by the Food and Drug Administration, at amounts of 0.1% by weight of food. However, previous reports indicate that SB exposure may induce histological alterations in kidney structures and promote oxidative stress. Thus, with increasing obesity rates and fatty diets, the effects of SB coupled to a high fat diet (HFD) remain to be determined. This study addressed the hypothesis that SB with a HFD will increase extracellular …

Investigating The Mechanisms Through Which Autophagy Controls Lipid Levels In Cyst Stem Cells., Jared Langdon

College of Arts & Sciences Senior Honors Theses

With an increase in the average age of the American population, incidents of age-related diseases and illnesses are rising across healthcare communities. Addressing these pathologies with a focus towards novel treatments warrants further research into their mechanistic origins. Lipid build up within cells has been correlated with increased risk for certain pathologies, namely Alzheimer's disease and type-2 diabetes (Demarco, 2020). In this study I aimed to investigate the origin of the increase in lipids, specifically we consider the effects of lipid build up on the maintenance of stem cell populations.

Innovative Virtual Wellness Interventions At An Academic Medical Center: A Pilot Feasibility Study, Ritika Baweja, Michael Hayes, Aditya Joshi, Raman Baweja

Journal of Wellness

Introduction: There is generally a concerning likelihood of burnout in healthcare workers. Given the impact of COVID-19 on healthcare workers, our institution identified the need for wellness interventions to foster adaptive functioning and mitigate burnout. The purpose of this pilot project was to assess the feasibility of virtual holistic interventions like meditation, art, laughter therapy and dance and their impact on overall well-being of physicians and advanced practice providers (APPs).

Methods: A series of 12 virtual sessions (art therapy, dance therapy, mindfulness-based practices/meditation and laughter therapy) were offered to providers over a 6-month period. Participants completed an online survey reporting …

Characterizing Leaf Endophyte Composition In The Dune Grass Ammophila Breviligulata, Nabaa Majeed, Sarah Hobbs, Sarah Emery, Connor Morozumi, Natalie Christian

Undergraduate Research Events

Microorganisms living within plants play crucial roles in their health and function, yet these diverse communities that make up the plant microbiome remain poorly understood. The dune grass Ammophila breviligulata. is an ecosystem engineer which helps to build and stabilize dune ecosystems effectively acting as a buffer against storms to coastal communities. It is currently unknown whether the Ammophila microbiome plays a role in this plant species’ ability to transform dune ecosystems.. In this study, we assessed fungal diversity of endophytes—microbes living within plant tissues— in Ammophila leaves collected from Leelanau State Park, MI along the eastern shores of Lake …

The Interplay Between Lung Adenocarcinoma, Nutrient Availability, And The Microbiome., Alexis A. Vega

Electronic Theses and Dissertations

Lung cancer is one of the most common forms of cancer diagnosed, and has the highest lethality rate across all other forms of cancer in the U.S. While current therapeutic options include chemo-, immuno-, and radio-therapy, the benefits of caloric or nutrient restriction on cancer cells has also been investigated. Due to the many biological functions associated with methionine, many have proposed a methionine restricted diet would lead to favorable outcomes when combating cancer. Although our cells are incapable of synthesizing methionine, the bacteria found in our microbiome can. Furthermore, recent discoveries suggest an independent microbiome found within tumors that …

Study Of The Effects Of Chronic Cadmium Exposure On The Pathogenesis Of Pulmonary Arterial Hypertension., Dakotah Dominique Cathey

Electronic Theses and Dissertations

We reported pulmonary artery hypertension (PAH) patients had elevated levels of Cd in both blood and urine, therefore, this study tested whether Cd directly induces PAH or facilitates PAH pathogenesis in mouse models. C57/6J mice were initially exposed to drinking water with or without 5ppm Cd for 8 weeks. Then, half the mice in both control and Cd groups were given SU5416 and hypoxia (SuHx) for 4 weeks to induce PAH, resulting in 4 subgroups: Control, Cd, PAH, and Cd+PAH. Diastolic and systolic functions of the left and right ventricles (LV, RV) were examined with echocardiography before and after PAH. …

Biocargos: Capture And Release Gels For Optimized Storage Of Biologics., Chinmay Shashank Potnis

Electronic Theses and Dissertations

No abstract provided.

The Impact Of Environmental Pollutants On Alcohol-Associated Liver Disease Pathogenesis., Tyler Charles Gripshover

Electronic Theses and Dissertations

Steatotic liver disease is brought upon by differing etiologies and its global prevalence is expected to keep increasing over the next several decades. Particularly, alcohol-associated liver disease (ALD) manifests from excessive alcohol consumption where the liver retains lipids and progresses with metabolic dysfunction. Previous studies have demonstrated that environmental pollutants, polychlorinated biphenyls (PCBs) promote metabolic dysfunction associated steatotic liver disease (MASLD), in diet-induced obesity models. However, it is unknown if environmental pollutants may also promote ALD pathogenesis. Thus, this dissertation focuses on characterizing the effects of PCB126 in a rodent ALD model followed by multiple ‘Omics approaches to identify mechanisms …

Using A Toxic Aging Coin To Assess Hexavalent Chromium-Induced Neurotoxicity., Samuel Thomas Vielee

Electronic Theses and Dissertations

We are facing an aging crisis, with 20% of the U.S. population projected to be geriatric (65+) by 2030 and live another 40+ years. Age-related diseases accompany a growing geriatric population, emphasizing the need to understand their etiology. Environmental pollutants compound this crisis by: 1) geriatrics are more susceptible, exacerbating age-related diseases and comorbidities, and 2) they accelerate biological aging, inducing age-related diseases at younger ages. We address this crisis using a ‘toxic aging coin’ approach; heads examines how age impacts toxicity, tails examines how chemicals accelerate aging. This thesis applies the heads side for Cr(VI)-induced neurotoxicity across ages. We …

Breaking Virulent: The Coincidental Evolution Of Virulence Factors In Bacteria., Rhiannon Emmanuelle Cecil

Electronic Theses and Dissertations

Understanding how innocuous organisms can evolve to be pathogenic to humans is of increasing global concern. Further, understanding how existing pathogens may evolved to be more virulent is also vital to our ability to provide healthcare to people afflicted with diseases that promote chronic bacterial infections, such as cystic fibrosis. With the rise of antibiotic resistance in both bacteria and fungi it is paramount that new therapeutics are identified. Understanding what mutations occur that result in increased virulence in microbes can potentially provide new targets for antimicrobial drugs to combat antibiotic resistance. The Coincidental Evolution Hypothesis is a fundamental hypothesis …

Effects Of A Protection Zone In A Reaction-Diffusion Model With Strong Allee Effect., Isaac Johnson

Electronic Theses and Dissertations

A protection zone model represents a patchy environment with positive growth over the protection zone and strong Allee effect growth outside the protection zone. Generally, these models are considered through the corresponding eigenvalue problem, but that has certain limitations. In this thesis, a general protection zone model is considered. This model makes no assumption on the direction of the traveling wave solution over the Strong Allee effect patch. We use phase portrait analysis of this protection zone model to draw conclusions about the existence of equilibrium solutions. We establish the existence of three types of equilibrium solutions and the necessary …

The Emerging Oral Pathogen, Filifactor Alocis, Modulates Antimicrobial Responses Of Primed Human Neutrophils., Ian J. Snider, Aruna Vashishta, Richard J. Lamont, Silvia M. Uriarte

The Cardinal Edge

Almost 50% of the adult population older than 30 years of age suffers from some form of periodontitis, a chronic inflammatory disease of the periodontal tissue caused by microbial subversion of the host immune response. Neutrophils are the most abundant leukocyte present in the oral mucosa. In periodontitis, periodontal pathogens have developed strategies to evade neutrophil antimicrobial responses and promote bacterial growth. Among these oral pathogens is Filifactor alocis which can modulate neutrophils’ antimicrobial responses by preventing phagosome maturation. During inflammation, neutrophils that reach the gingival tissue are primed by cytokines and chemokines. However, the response of primed human neutrophils …

Hyaluronan Modulated Expression Of Mmps 2, 9, And 12 In Macrophages, Jeffrey S. Miller, Caitlin Howard, Steven P. Jones Phd

The Cardinal Edge

Hyaluronan (HA) is a ubiquitously expressed Glycosaminoglycan (GAG) found as a main component of the extracellular matrix (ECM). Matrix metalloproteases (MMPs) are class of enzyme responsible for the degradation of multiple ECM components, including HA. Following a myocardial infarction (MI), ECM remodeling occurs in the infarct tissue and involves an accumulation of HA. Remodeling is facilitated by multiple cell types, including macrophages. During post-MI ECM remodeling, macrophages degrade and ingulf dead cells and ECM components, a process which requires the expression of MMPs. MMPs 2, 9, and 12 are known to be elevated post-MI; MMPs 9 and 12 are known …

The Neural Sequalae Of Subjectively Experiencing Autobiographical Memories From The Remote Past And Recent Present Using Fmri, Ava G. Peruski, Nim Singh, Brendan E. Depue

The Cardinal Edge

Autobiographical memory is central to one's sense of self and continuity from past to present. Despite this, there is little research on the neural correlates underlying individual subjective experience of autobiographical memory and how that is related to brain phenomena (i.e., activity, communication). The purpose of this study was to help minimize this gap. We recruited twenty healthy adult participants, who were asked to generate memory cues (1-3 word descriptions) for locations and objects from their early and recent life. After 24 hours, participants were shown these cues then asked to recall the appropriate memory while in an fMRI scanner. …